Jiang, L.; Dumlao, M.C.; Donald, W.A.; Steel, C.C.; Schmidtke, L.M. Rapid In-Field Volatile Sampling for Detection of Botrytis cinerea Infection in Wine Grapes. Molecules2023, 28, 5227.
Jiang, L.; Dumlao, M.C.; Donald, W.A.; Steel, C.C.; Schmidtke, L.M. Rapid In-Field Volatile Sampling for Detection of Botrytis cinerea Infection in Wine Grapes. Molecules 2023, 28, 5227.
Jiang, L.; Dumlao, M.C.; Donald, W.A.; Steel, C.C.; Schmidtke, L.M. Rapid In-Field Volatile Sampling for Detection of Botrytis cinerea Infection in Wine Grapes. Molecules2023, 28, 5227.
Jiang, L.; Dumlao, M.C.; Donald, W.A.; Steel, C.C.; Schmidtke, L.M. Rapid In-Field Volatile Sampling for Detection of Botrytis cinerea Infection in Wine Grapes. Molecules 2023, 28, 5227.
Abstract
Fungal infection of grape berries (Vitis vinifera) by Botrytis cinerea frequently coincides with harvest, impacting both the yield and quality of grape and wine products. A rapid and non-destructive method for identifying B. cinerea infection in grapes at an early stage prior to harvest is critical to manage loss. In this study, zeolitic imidazolate framework-8 (ZIF-8) crystal was applied as an absorbent material for volatile extraction from B. cinerea-infected and healthy grapes in a vineyard followed by thermal desorption gas chromatography-mass spectrometry. The performance of ZIF-8 to absorb and trap targeted volatiles was evaluated with a standard solution of compounds and with a whole bunch of grapes enclosed in a glass container to maintain standard sampling conditions. Results from sampling methods were then correlated to B. cinerea infection in grapes as measured and determined by Genus specific antigen quantification. Trace levels of targeted compounds reported as markers of grape B. cinerea infection were successfully detected with in-field sampling. Peak area counts for volatiles 3-octanone, 1-octen-3-one, 3-octanol, and 1-octen-3-ol extracted using ZIF-8 were significantly higher than values achieved using Tenax®-TA from field testing and demonstrated good correlation with B. cinerea infection severities determined by B. cinerea antigen detection.
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