Fernandez-Lopez, L.; Roda, S.; Robles-Martín, A.; Muñoz-Tafalla, R.; Almendral, D.; Ferrer, M.; Guallar, V. Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering. Int. J. Mol. Sci.2023, 24, 13768.
Fernandez-Lopez, L.; Roda, S.; Robles-Martín, A.; Muñoz-Tafalla, R.; Almendral, D.; Ferrer, M.; Guallar, V. Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering. Int. J. Mol. Sci. 2023, 24, 13768.
Fernandez-Lopez, L.; Roda, S.; Robles-Martín, A.; Muñoz-Tafalla, R.; Almendral, D.; Ferrer, M.; Guallar, V. Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering. Int. J. Mol. Sci.2023, 24, 13768.
Fernandez-Lopez, L.; Roda, S.; Robles-Martín, A.; Muñoz-Tafalla, R.; Almendral, D.; Ferrer, M.; Guallar, V. Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering. Int. J. Mol. Sci. 2023, 24, 13768.
Abstract
Lipases have tremendous potential for industrial use, particularly, those mostly active against water-insoluble substrates, such as triglycerides composed of long-chain fatty acids. However, in most cases, mutants often need to be constructed to achieve optimal performance for such substrates. Protein engineering techniques have been reported as strategies for improving lipase characteristics by introducing specific mutations in the cap domain of esterases or in the lid domain of lipases, or through lid domain swapping. Here, we have improved the lipase character of a lipase, retrieved from the Marine Metagenomics MarRef Database and assigned to the Actinoalloteichus genus (WP_075743487.1), through site-directed mutagenesis and by substituting its lid domain (FRGTEITQIKDWLTDA) by the one of Rhizopus delemar (previously Rhizopus oryzae) lipase (FRGTNSFRSAITDIVF). Results demonstrated that the redesigned mutants gain activity against bulkier triglycerides such as glyceryl tridecanoate and tridodecanoate, olive oil, coconut oil, and palm oil. The absence of a residue in the new lid, present in the original lid, appears to be a key aspect to the increase in lipase character, although full activity recovery is only obtained with lid swapping.
Keywords
lipase; lid domain; protein engineering; rational design
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.