Iqbal, J.; Ashraf, M. A.; Cai, Q.; Riaz, A.; Li, Y.; Shahid, I.; Kong, Y.; Grewal, A. G.; Kiran, S.; Rehman, S.; Habib, I.; Yu, N. Simple Sequence Repeats-Based DNA Fingerprinting and Varietal Identification of Mango Cultivars. Preprints2023, 2023091723. https://doi.org/10.20944/preprints202309.1723.v1
APA Style
Iqbal, J., Ashraf, M. A., Cai, Q., Riaz, A., Li, Y., Shahid, I., Kong, Y., Grewal, A. G., Kiran, S., Rehman, S., Habib, I., & Yu, N. (2023). Simple Sequence Repeats-Based DNA Fingerprinting and Varietal Identification of Mango Cultivars. Preprints. https://doi.org/10.20944/preprints202309.1723.v1
Chicago/Turabian Style
Iqbal, J., Imran Habib and Naitong Yu. 2023 "Simple Sequence Repeats-Based DNA Fingerprinting and Varietal Identification of Mango Cultivars" Preprints. https://doi.org/10.20944/preprints202309.1723.v1
Abstract
Assessment of the genetic distinctiveness of a cultivar through morphological descriptors is an important tool for both the registration and the protection. New mango genotypes have been improved using valuable diverse germplasm resources to ensure food security. DNA fingerprinting based simple sequence repeats (SSR)-markers have been the most broadly used, effective and accurate in evaluation of genetic characterization of a cultivar. Molecular breeding is an effective source of genetic gain after improvement of fruit trees using marker assisted genomic selection. Mango (Mangifera indica L.) is an allotetraploid (2n = 4X= 40) drupe fruit and has high nutritional value belongs to genus Mangifera and family Anacardiaceae. Mango cultivars are used with worldwide acceptance to pharmacological, ethnomedical, and phytochemical industries. This study investigated the molecular evaluation of a new mango cultivar ‘Azeem Chaunsa’ using a set of the most effective 50 hyper-variable polymorphic SSR markers. Highly specific DNA fingerprints were identified in the genome of this mango cultivar, ‘Azeem Chaunsa’ compared with three standard cultivars such as Sindhri, Samar Bahisht (S.B) Chaunsa and Sufaid Chaunsa. Our results showed that SSR markers could efficiently assess genetic diversity in mango. An agglomerative hierarchical clustering method was used to construct dendrogram based on the ‘Unweighted Pair- Group Method with Arithmetic Mean’ (UPGMA). The genetic similarity coefficients were recorded between the mangos cultivars ranged from 0.49 to 0.67. Cultivar identification (CID) evaluates association among standard cultivars and Azeem Chaunsa and further concludes significant variations. CID results concluded that cultivar ‘Azeem Chaunsa’ varied significantly from the check cultivar, Sindhri (46.2%), S.B Chaunsa (45%) and Sufaid Chaunsa (46.7%). The results obtained in this study will orient cultivar identification strategies for a successful future mango breeding programmes in the context of climate change.
Keywords
Mango tree; SSR markers; mango varieties; genetic diversity; cultivar identification diagram, loci, polymorphism; genetic resources in Pakistan; breeding; cultivar development
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
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