Glazieva, V.S.; Alexandrushkina, N.A.; Nimiritsky, P.P.; Kulebyakina, M.A.; Eremichev, R.Y.; Makarevich, P.I. Extracellular Matrix Deposition Defines the Duration of Cell Sheet Assembly from Human Adipose-Derived MSC. Int. J. Mol. Sci.2023, 24, 17050.
Glazieva, V.S.; Alexandrushkina, N.A.; Nimiritsky, P.P.; Kulebyakina, M.A.; Eremichev, R.Y.; Makarevich, P.I. Extracellular Matrix Deposition Defines the Duration of Cell Sheet Assembly from Human Adipose-Derived MSC. Int. J. Mol. Sci. 2023, 24, 17050.
Glazieva, V.S.; Alexandrushkina, N.A.; Nimiritsky, P.P.; Kulebyakina, M.A.; Eremichev, R.Y.; Makarevich, P.I. Extracellular Matrix Deposition Defines the Duration of Cell Sheet Assembly from Human Adipose-Derived MSC. Int. J. Mol. Sci.2023, 24, 17050.
Glazieva, V.S.; Alexandrushkina, N.A.; Nimiritsky, P.P.; Kulebyakina, M.A.; Eremichev, R.Y.; Makarevich, P.I. Extracellular Matrix Deposition Defines the Duration of Cell Sheet Assembly from Human Adipose-Derived MSC. Int. J. Mol. Sci. 2023, 24, 17050.
Abstract
Cell sheet (CS) engineering using human MSC is of significant interest for regenerative medicine and this rapidly growing field translates to clinical use in a number of indications. Nevertheless, little is known of factors that define the timing of CS assembly from primary cultures which is important for planning treatments in both – autologous and allogeneic modes of use. We used a comparative in vitro approach with primary donors’ (n=14) adipose-derived MSC and evaluated the impact of healthy subject’s characteristics (age and sex), MSC culture features (population doubling time and lag-phase) and extracellular matrix (ECM) composition along with factors related to connective tissue formations (-SMA and FAP-) on CS assembly duration.
Biology and Life Sciences, Cell and Developmental Biology
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