Version 1
: Received: 18 October 2023 / Approved: 18 October 2023 / Online: 19 October 2023 (12:06:07 CEST)
Version 2
: Received: 17 November 2023 / Approved: 20 November 2023 / Online: 21 November 2023 (07:10:14 CET)
How to cite:
BAMIDELE, F.; Okonji, R.; Chinenye, I.; Okanlawon, K. Unlocking the Biodegradation Potential: Comparing Cellulase Activity from Unconventional Sources - Rhinoceros Beetle Larvae and Giant African Snails. Preprints2023, 2023101255. https://doi.org/10.20944/preprints202310.1255.v2
BAMIDELE, F.; Okonji, R.; Chinenye, I.; Okanlawon, K. Unlocking the Biodegradation Potential: Comparing Cellulase Activity from Unconventional Sources - Rhinoceros Beetle Larvae and Giant African Snails. Preprints 2023, 2023101255. https://doi.org/10.20944/preprints202310.1255.v2
BAMIDELE, F.; Okonji, R.; Chinenye, I.; Okanlawon, K. Unlocking the Biodegradation Potential: Comparing Cellulase Activity from Unconventional Sources - Rhinoceros Beetle Larvae and Giant African Snails. Preprints2023, 2023101255. https://doi.org/10.20944/preprints202310.1255.v2
APA Style
BAMIDELE, F., Okonji, R., Chinenye, I., & Okanlawon, K. (2023). Unlocking the Biodegradation Potential: Comparing Cellulase Activity from Unconventional Sources - Rhinoceros Beetle Larvae and Giant African Snails. Preprints. https://doi.org/10.20944/preprints202310.1255.v2
Chicago/Turabian Style
BAMIDELE, F., Isaac Chinenye and Kehinde Okanlawon. 2023 "Unlocking the Biodegradation Potential: Comparing Cellulase Activity from Unconventional Sources - Rhinoceros Beetle Larvae and Giant African Snails" Preprints. https://doi.org/10.20944/preprints202310.1255.v2
Abstract
The global predicament of solid waste generation looms as a mounting crisis, profoundly impacting the world, particularly in developing nations. Each day, the world's population contributes millions of tonnes of municipal solid waste, with projections pointing toward further escalation in the coming years. This surge in waste, predominantly composed of cellulose-rich materials, correlates directly with heightened pollution, a threat that transcends borders and imperils humanity as a whole.To confront this ever-growing issue, cellulase, a potent hydrolytic enzyme capable of digesting cellulose, a chief component of solid waste, is explored as a potential solution. In this study, we harness cellulase enzymes derived from the digestive tracts of both Archachatina marginata and Oryctes rhinoceros larvae, employing them as biodegradation agents for cellulose-based waste materials.The cellulase enzymes are methodically isolated and partially purified via ammonium sulfate precipitation at 65% saturation. Their efficacy in breaking down a variety of waste categories, encompassing kitchen waste, leaves, paper, wood, and industrial waste, is meticulously assessed and benchmarked against carboxymethyl cellulose (CMC), a well-established laboratory substrate. The study also ascertains the specific activity of these enzymes.Spectrophotometric analyses of the cellulase enzymes' actions on the diverse waste materials unveil intriguing results. Archachatina marginata and Oryctes rhinoceros larvae exhibit distinct performance metrics, registering percentage ranges of (11.04% - 28.31%) and (33.69% - 189.11%) on wood waste, (76.52% - 196%) and (108.56% - 567.01%) on paper waste, (78.57% - 148.14%) and (163.33% - 436.76%) on leaf waste, (57.16% - 179.95%) and (63.38% - 457.05%) on kitchen waste, and (59.95% - 77.51%) and (117.26% - 155.23%) on industrial waste, respectively. This study underscores the substantial biodegradation potential inherent in cellulase enzymes from both Oryctes rhinoceros larvae and Archachatina marginata when applied to diverse waste materials. While the rates and impacts of waste degradation vary between these sources, their promising contributions to waste management come into focus, offering sustainable and innovative approaches to tackle the pressing global challenge of solid waste proliferation.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
21 November 2023
Commenter:
Isaac Ugwu
Commenter's Conflict of Interests:
Author
Comment: updates on the authors, as I inadvertently omitted some of them. I apologize for this oversight. Additionally, minor adjustments have been made to document spacing for improved readability.
Commenter: Isaac Ugwu
Commenter's Conflict of Interests: Author