Suzuki, H., Tawara, M., Hirayama, A., Goto, N., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) Using Enzyme-Linked Immunosorbent Assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Suzuki, H., Tawara, M., Hirayama, A., Goto, N., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) Using Enzyme-Linked Immunosorbent Assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Suzuki, H., Tawara, M., Hirayama, A., Goto, N., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) Using Enzyme-Linked Immunosorbent Assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Suzuki, H., Tawara, M., Hirayama, A., Goto, N., Tanaka, T., Kaneko, M. K., & Kato, Y. (2024). Epitope Mapping of an Anti-CD44v4 Monoclonal Antibody (C44Mab-108) Using Enzyme-Linked Immunosorbent Assay. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.
Abstract
CD44 is a type I transmembrane glycoprotein, and possesses various isoforms which are largely classified into CD44 standard and CD44 variant (CD44v) isoforms. Some variant-encoded regions play critical roles in tumor progression. However, the function of CD44 variant 4 (CD44v4)-encoded region has not been fully understood. Using peptide immunization, we developed an anti-CD44v4 mAb, C44Mab-108, which is useful for flow cytometry, western blotting, and immunohistochemistry. In this study, we determined the critical epitope of C44Mab-108 by enzyme-linked immunosorbent assay (ELISA). We used the alanine(or glycine)-substituted peptides of the CD44v4-encoded region(amino acids 271-290 of human CD44v3-10), and found that C44Mab-108 did not recognize the alanine-substituted peptides of D280A and W281A. Furthermore, these peptides could not inhibit the recognition of C44Mab-108 in flow cytometry and immunohistochemistry. The results indicate that the critical binding epitope of C44Mab-108 includes Asp280 and Trp281 of CD44v3-10.
Medicine and Pharmacology, Oncology and Oncogenics
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