Shabani, Z.; Schuerger, J.; Zhu, X.; Tang, C.; Ma, L.; Yadav, A.; Liang, R.; Press, K.; Weinsheimer, S.; Schmidt, A.; Wang, C.; Sekhar, A.; Nelson, J.; Kim, H.; Su, H. Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells2024, 13, 92.
Shabani, Z.; Schuerger, J.; Zhu, X.; Tang, C.; Ma, L.; Yadav, A.; Liang, R.; Press, K.; Weinsheimer, S.; Schmidt, A.; Wang, C.; Sekhar, A.; Nelson, J.; Kim, H.; Su, H. Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells 2024, 13, 92.
Shabani, Z.; Schuerger, J.; Zhu, X.; Tang, C.; Ma, L.; Yadav, A.; Liang, R.; Press, K.; Weinsheimer, S.; Schmidt, A.; Wang, C.; Sekhar, A.; Nelson, J.; Kim, H.; Su, H. Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells2024, 13, 92.
Shabani, Z.; Schuerger, J.; Zhu, X.; Tang, C.; Ma, L.; Yadav, A.; Liang, R.; Press, K.; Weinsheimer, S.; Schmidt, A.; Wang, C.; Sekhar, A.; Nelson, J.; Kim, H.; Su, H. Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse. Cells 2024, 13, 92.
Abstract
Background: Increase of collagen I (COL I)/COL III ratio enhances vessel wall stiffness and renders vessels less resistant to blood flow and pressure changes. We hypothesized that COL I/COL III ratio is increased in the brain arteriovenous malformations (bAVMs), which is associated with bAVM hemorrhage. Method: Surgically resected human bAVM samples and mouse bAVMs induced in three transgenic mouse lines with activin receptor-like kinase 1 or endoglin deletion in the endothelial cells or in the brain focally. Colony-stimulating factor receptor inhibitor was used to transiently deplete microglia. COL I and COL III levels, hemorrhage, and gene expression in bAVMs were analyzed. Results: The COL I and COL III levels and the COL I/COL III ratio were higher on human and mouse bAVMs than controls, which was associated with bAVM hemorrhage. Col I and Col III mRNAs were also increased in mouse bAVMs. The degree of microhemorrhage in mouse bAVMs was positively correlated with Col I/Col III ratios. Transient depletion of microglia reduced Col I/Col III ratio and microhemorrhage in mouse bAVMs. Conclusion: COL I/COL III ratio are increased in bAVM vessels, which is associated with bAVM hemorrhage. Depletion of microglia reduces Col I/Col III ratio and hemorrhage in mice bAVMs.
Keywords
Brain arteriovenous malformation; Collagen I; Collagen III; Microhemorrhage; Depletion of microglia; Gluycoprotein nonmetastatic B
Subject
Biology and Life Sciences, Other
Copyright:
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