Article
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Ethanol Based Extraction of Annatto (Bixa Orellana L.) and Characterisation of the Bixin and Norbixin
Version 1
: Received: 18 December 2023 / Approved: 19 December 2023 / Online: 20 December 2023 (01:37:34 CET)
A peer-reviewed article of this Preprint also exists.
Aluko, E. (2024). Ethanol-Based Extraction of Annatto (Bixa Orellana L.) and Characterization of the Bixin and Norbixin. ACS omega. Aluko, E. (2024). Ethanol-Based Extraction of Annatto (Bixa Orellana L.) and Characterization of the Bixin and Norbixin. ACS omega.
Abstract
Bixa orellana dye was extracted from the seed by method of Soxhlet extraction using ethanol and a percentage yield of 4.3 was obtained. Recrystallisation of the dye was performed using chloroform and ethanol. TLC was used to separate the dye into Bixin and Norbixin with Rf values of 0.56 and 0.42 respectively. The extracted dye was pH sensitive, insoluble in water but soluble in some organic solvents like ethanol and acetic acid . Characterisation by UV-spectroscopy showed maximum absorption for Bixin and Norbixin at 457nm and 453nm respectively. FTIR spectrum of Bixin and Norbixin showed bands confirming similar functional groups for both Bixin and Norbixin except for –CO- stretching and methylene vibration which was absent in Norbixin.
Keywords
Soxhlet; Characterisation; ethanol; pH; Spectroscopy; UV; FTIR; Bixin; Norbixin
Subject
Chemistry and Materials Science, Other
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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