Article
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Improved Extraction Method of Soil Nitrite
Version 1
: Received: 27 December 2023 / Approved: 27 December 2023 / Online: 27 December 2023 (14:37:16 CET)
A peer-reviewed article of this Preprint also exists.
Song, Y.; Wu, D.; Dörsch, P.; Yue, L.; Deng, L.; Liao, C.; Sha, Z.; Dong, W.; Yu, Y. Improved Method for Extracting Nitrites in Soil. Agronomy 2024, 14, 331. Song, Y.; Wu, D.; Dörsch, P.; Yue, L.; Deng, L.; Liao, C.; Sha, Z.; Dong, W.; Yu, Y. Improved Method for Extracting Nitrites in Soil. Agronomy 2024, 14, 331.
Abstract
Soil nitrite (NO2‒) is an important reactive intermediate in many nitrogen transformation processes, but it is unstable under acidic conditions. Canonical extraction method of soil NO2‒ with potassium chloride (KCl) solution greatly underestimates its concentration. In order to reflect the concentration more accurately, we optimized the extraction method of soil NO2‒ in this study. Moreover, soil ammonium (NH4+) and nitrate (NO3‒) were also systematically investigated to achieve efficient extraction of soil inorganic nitrogen. The results showed that un-buffered KCl significantly underestimated soil NO2‒ concentration compared to DIW. The highest recovery of NO2‒ was obtained by extracting with DIW at 10 min of oscillation for three soils. Compared with DIW, the concentration of NH4+ and NO3‒ in soil extracted from KCl solution increased significantly. Furthermore, the soil inorganic nitrogen content of extracts stored at 4°C for one day were closer to directly measurements of fresh samples than the other storage methods. Overall, the recommend analysis method for soil NO2‒ was extracted by DIW, oscillated for 10 min, and filtered by a 0.45 µm filter, and soil NH4+ and NO3‒ were extracted with KCl solution and oscillated for 30 min. The extract should be stored at 4°C and analyzed within 24 hours.
Keywords
soil nitrite; deionized water; potassium chloride; oscillation time; storage duration
Subject
Environmental and Earth Sciences, Soil Science
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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