Shen, W.; Pan, L.; Fu, Y.; Suo, Y.; Zhang, Y.; Liu, H.; Su, X.; Zhao, J. Comparative Study on the Effectiveness of Three Inoculation Methods for Valsa sordida in Populus alba var. pyramidalis. Biology2024, 13, 251.
Shen, W.; Pan, L.; Fu, Y.; Suo, Y.; Zhang, Y.; Liu, H.; Su, X.; Zhao, J. Comparative Study on the Effectiveness of Three Inoculation Methods for Valsa sordida in Populus alba var. pyramidalis. Biology 2024, 13, 251.
Shen, W.; Pan, L.; Fu, Y.; Suo, Y.; Zhang, Y.; Liu, H.; Su, X.; Zhao, J. Comparative Study on the Effectiveness of Three Inoculation Methods for Valsa sordida in Populus alba var. pyramidalis. Biology2024, 13, 251.
Shen, W.; Pan, L.; Fu, Y.; Suo, Y.; Zhang, Y.; Liu, H.; Su, X.; Zhao, J. Comparative Study on the Effectiveness of Three Inoculation Methods for Valsa sordida in Populus alba var. pyramidalis. Biology 2024, 13, 251.
Abstract
A key step in the study of tree pathology is the identification of appropriate method for inoculating pathogens of diseases in branch and trunk. Pathogens of diseases in branch and trunk are commonly inoculated through punching, burning, and toothpick inoculation. However, there is a lack of comparative analysis of the inoculation outcomes of these three methods. In this work, Six‐year‐old P. alba var. pyramidalis were inoculated with V. sordida, using punching, burning, and toothpick techniques, respectively, to investigate the differences in the effectiveness of these inoculation methods. Results reveal that the incidence rate was 93.55% in the toothpick inoculation group, significantly higher than 80.65% in the burning inoculation group (p = 0.007), while punching inoculation exhibited significant pathological responses in the early stages, with spontaneous healing in the later stage. Addi-tionally, toothpick inoculation was more efficient in inducing Valsa canker when inoculating the pathogen at the bottom of the tree, with lower intra‐ and inter‐row spacing (stand density) providing better outcomes than higher intra‐ and inter‐row spacing. The results of this study demonstrate that toothpick inoculation is an optimal option for studying the artificial inoculation of V. sordida in six‐year‐old P. alba var. pyramidalis, providing technical support for research on poplar diseases and offering a theoretical basis for the inoculation of other diseases in branch and trunk.
Keywords
punching inoculation; burning inoculation; toothpick inoculation; inoculation position
Subject
Biology and Life Sciences, Forestry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.