Version 1
: Received: 19 February 2024 / Approved: 6 March 2024 / Online: 6 March 2024 (11:04:56 CET)
How to cite:
Shin, E.; Yun, J.-S.; Lee, Y.-R.; Cha, H.-R.; Kim, S.-M.; Shin, S.-J.; Lee, S.-W.; Chung, G. T.; Kim, D.; Yoo, J. S.; Kim, J.-S.; Jeong, H.-S. Efficacy and Immunogenicity of rKVAC85B in a BCG Prime-Boost Regimen Against H37Rv and HN878 Tuberculosis Strains. Preprints2024, 2024030351. https://doi.org/10.20944/preprints202403.0351.v1
Shin, E.; Yun, J.-S.; Lee, Y.-R.; Cha, H.-R.; Kim, S.-M.; Shin, S.-J.; Lee, S.-W.; Chung, G. T.; Kim, D.; Yoo, J. S.; Kim, J.-S.; Jeong, H.-S. Efficacy and Immunogenicity of rKVAC85B in a BCG Prime-Boost Regimen Against H37Rv and HN878 Tuberculosis Strains. Preprints 2024, 2024030351. https://doi.org/10.20944/preprints202403.0351.v1
Shin, E.; Yun, J.-S.; Lee, Y.-R.; Cha, H.-R.; Kim, S.-M.; Shin, S.-J.; Lee, S.-W.; Chung, G. T.; Kim, D.; Yoo, J. S.; Kim, J.-S.; Jeong, H.-S. Efficacy and Immunogenicity of rKVAC85B in a BCG Prime-Boost Regimen Against H37Rv and HN878 Tuberculosis Strains. Preprints2024, 2024030351. https://doi.org/10.20944/preprints202403.0351.v1
APA Style
Shin, E., Yun, J. S., Lee, Y. R., Cha, H. R., Kim, S. M., Shin, S. J., Lee, S. W., Chung, G. T., Kim, D., Yoo, J. S., Kim, J. S., & Jeong, H. S. (2024). Efficacy and Immunogenicity of rKVAC85B in a BCG Prime-Boost Regimen Against H37Rv and HN878 Tuberculosis Strains. Preprints. https://doi.org/10.20944/preprints202403.0351.v1
Chicago/Turabian Style
Shin, E., Jong-Seok Kim and Hye-Sook Jeong. 2024 "Efficacy and Immunogenicity of rKVAC85B in a BCG Prime-Boost Regimen Against H37Rv and HN878 Tuberculosis Strains" Preprints. https://doi.org/10.20944/preprints202403.0351.v1
Abstract
Mycobacterium tuberculosis was responsible for 1.3 million deaths worldwide in 2022. Bacillus Calmette-Guérin (BCG) is the only licensed vaccine against tuberculosis (TB); however, it has limited protective efficacy in adults. In this study, we constructed a recombinant vaccinia virus expressing Ag85B from M. tuberculosis using a novel attenuated vaccinia virus (KVAC103). We then analyzed the immunogenicity of prime-boost inoculation strategies using recombinant KVAC103 expressing Ag85B (rKVAC85B) compared to BCG. In both the rKVAC85B prime-boost and the BCG prime-rKVAC85B boost inoculation regimens, rKVAC85B induced the generation of specific IgG and the secretion of IFN-γ by immune cells. In vitro analysis of Mycobacterium growth inhibition revealed a comparable immune-mediated pattern of outcomes. Furthermore, bacterial loads in the lungs were significantly lower in mice inoculated with the BCG prime-rKVAC85B boost, than in the BCG-only group following a rechallenge infection with both H37Rv and HN878 strains of M. tuberculosis. These findings collectively suggest that KVAC103, incorporated into a viral vector, is a promising candidate for a novel TB vaccine platform that is effective against multiple TB strains, including H37Rv and HN878, and that rKVAC85B effectively stimulates immune responses against TB infection.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.