Jung, S.-B.; Choi, G.; Kim, H.-J.; Moon, K.-S.; Lee, G.; Na, K.-H.; Kwon, Y.M.; Moon, J.; Shin, M.Y.; Yu, J.-Y.; Baek, Y.-B.; Park, J.-G.; Park, S.-I. A Noble Extract of Pseudomonas sp. M20A4R8 Efficiently Controlling the Influenza Virus-Induced Cell Death. Microorganisms2024, 12, 677.
Jung, S.-B.; Choi, G.; Kim, H.-J.; Moon, K.-S.; Lee, G.; Na, K.-H.; Kwon, Y.M.; Moon, J.; Shin, M.Y.; Yu, J.-Y.; Baek, Y.-B.; Park, J.-G.; Park, S.-I. A Noble Extract of Pseudomonas sp. M20A4R8 Efficiently Controlling the Influenza Virus-Induced Cell Death. Microorganisms 2024, 12, 677.
Jung, S.-B.; Choi, G.; Kim, H.-J.; Moon, K.-S.; Lee, G.; Na, K.-H.; Kwon, Y.M.; Moon, J.; Shin, M.Y.; Yu, J.-Y.; Baek, Y.-B.; Park, J.-G.; Park, S.-I. A Noble Extract of Pseudomonas sp. M20A4R8 Efficiently Controlling the Influenza Virus-Induced Cell Death. Microorganisms2024, 12, 677.
Jung, S.-B.; Choi, G.; Kim, H.-J.; Moon, K.-S.; Lee, G.; Na, K.-H.; Kwon, Y.M.; Moon, J.; Shin, M.Y.; Yu, J.-Y.; Baek, Y.-B.; Park, J.-G.; Park, S.-I. A Noble Extract of Pseudomonas sp. M20A4R8 Efficiently Controlling the Influenza Virus-Induced Cell Death. Microorganisms 2024, 12, 677.
Abstract
Epidemic diseases that arise from infectious RNA viruses, particularly influenza viruses, pose a constant threat to the global economy and public health. Viral evolution has undermined the efficacy of acquired immunity from vaccines and the antiviral effects of FDA-approved drugs. As such, there is an urgent need to develop new antiviral lead agents. Natural compounds, owing to their historical validation of application and safety, have become a promising solution. In this light, a novel marine bacterium, Pseudomonas sp. M20A4R8, has been found to exhibit significant antiviral activity [half maximal inhibitory concentration (IC50) = 1.3 µg/mL, selectivity index (SI) = 919.4] against influenza virus A/Puerto Rico/8/34, surpassing the activity of chloroquine. The antiviral response by M20A4R8 extract was induced during post-entry stages of the influenza virus, indicating suitability for post-application after the establishment of viral infection. Furthermore, post-treatment with M20A4R8 extract protected the host from virus-induced apoptosis, suggesting its potential use in acute respiratory disease complexes resulting from immune effectors' overstimulation and autophagy-mediated self-apoptosis. The extract demonstrated an outstanding therapeutic index against influenza virus A/Wisconsin/15/2009 (IC50 = 8.1 µg/mL, SI = 146.2) and B/Florida/78/2015 Victoria lineage (IC50 = 3.5 µg/mL, SI = 343.8), indicating a broad anti-influenza virus activity with guaranteed safety and effectiveness. This study provides a new perspective on mechanisms for preventing a broad spectrum of viral infections through antiviral agents from novel and natural origins. Future studies on a single or combined compound from the extract hold promise, encouraging its use in preclinical challenge tests with various influenza virus strains.
Keywords
Pseudomonas; influenza virus; broad-spectrum therapeutics; marine bacterium; cell death
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.