Sancha Dominguez, L.; Cotos Suárez, A.; Sánchez Ledesma, M.; Muñoz Bellido, J.L. Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis. Diagnostics 2024, 14, 931, doi:10.3390/diagnostics14090931.
Sancha Dominguez, L.; Cotos Suárez, A.; Sánchez Ledesma, M.; Muñoz Bellido, J.L. Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis. Diagnostics 2024, 14, 931, doi:10.3390/diagnostics14090931.
Sancha Dominguez, L.; Cotos Suárez, A.; Sánchez Ledesma, M.; Muñoz Bellido, J.L. Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis. Diagnostics 2024, 14, 931, doi:10.3390/diagnostics14090931.
Sancha Dominguez, L.; Cotos Suárez, A.; Sánchez Ledesma, M.; Muñoz Bellido, J.L. Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis. Diagnostics 2024, 14, 931, doi:10.3390/diagnostics14090931.
Abstract
A Infectious diseases account for about 3 million deaths per year. The advent of molecular techniques has led to an enormous improvement in their diagnosis, both in terms of sensitivity and specificity and in terms of the speed with which a clinically useful result can be obtained. Digital PCR, or 3rd generation PCR, is based on a series of technical modifications that result in more sensitive techniques, more resistant to the action of inhibitors and capable of direct quantification, without the need for standard curves.This review presents the main applications that have been developed for the diagnosis of viral, bacterial and parasitic infections, and the potential prospects for the clinical use of this technology.
Keywords
dPCR; digital PCR; infection; bacteria; virus; parasites
Subject
Medicine and Pharmacology, Epidemiology and Infectious Diseases
Copyright:
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