Version 1
: Received: 7 May 2024 / Approved: 9 May 2024 / Online: 13 May 2024 (04:21:57 CEST)
How to cite:
Rio, P. D.; DiMarco, S.; Madan, P. MicroRNAomic Analysis of Spent Media From Slow and Fast-Growing Bovine Embryos Reveal Distinct Differences. Preprints2024, 2024050596. https://doi.org/10.20944/preprints202405.0596.v1
Rio, P. D.; DiMarco, S.; Madan, P. MicroRNAomic Analysis of Spent Media From Slow and Fast-Growing Bovine Embryos Reveal Distinct Differences. Preprints 2024, 2024050596. https://doi.org/10.20944/preprints202405.0596.v1
Rio, P. D.; DiMarco, S.; Madan, P. MicroRNAomic Analysis of Spent Media From Slow and Fast-Growing Bovine Embryos Reveal Distinct Differences. Preprints2024, 2024050596. https://doi.org/10.20944/preprints202405.0596.v1
APA Style
Rio, P. D., DiMarco, S., & Madan, P. (2024). MicroRNAomic Analysis of Spent Media From Slow and Fast-Growing Bovine Embryos Reveal Distinct Differences. Preprints. https://doi.org/10.20944/preprints202405.0596.v1
Chicago/Turabian Style
Rio, P. D., Sierra DiMarco and Pavneesh Madan. 2024 "MicroRNAomic Analysis of Spent Media From Slow and Fast-Growing Bovine Embryos Reveal Distinct Differences" Preprints. https://doi.org/10.20944/preprints202405.0596.v1
Abstract
In bovine embryos, microRNA (miRNA) expression has been profiled at each stage of early development in-vitro. miRNAomic analysis of spent media has the potential to reveal characteristics of embryo health, however, applications are limited without categorizing miRNA profiles by embryo quality. Time-lapse imaging has shown the timing of embryo development in-vitro may be indicative of their developmental potential. The aim of the study was to profile miRNAs in the spent media of slow and fast-growing bovine embryos throughout the pre-implantation period. Bovine cumulus-oocyte-complexes were aspirated from ovaries, fertilized, and cultured to blastocyst stage of development. At 2-cell, 8-cell, and blastocyst stage, each microdrop of 30 presumptive-zygotes were classified as slow or fast-growing based on the percentage of embryos that had reached the desired morphological stage. Following hybridization on a GeneChip miRNA 4.0 array, comparative analysis was conducted between spent media of slow and fast-growing embryos. In total, 34 differentially expressed miRNAs were identified between the comparison groups, with 14 of the miRNAs detected in the 2-cell samples, 7 miRNAs detected in the 8-cell samples, and 12 miRNAs detected in the blastocyst samples. The results demonstrate distinct miRNAs populations can be identified between slow and fast-growing embryos, highlighting novel biomarkers of developmental potential at each stage of pre-implantation development.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
