Vardakas, A.; Kechagias, A.; Penov, N.; Giannakas, A.E. Optimization of Enzymatic Assisted Extraction of Bioactive Compounds from Olea europaea Leaves. Biomass2024, 4, 647-657.
Vardakas, A.; Kechagias, A.; Penov, N.; Giannakas, A.E. Optimization of Enzymatic Assisted Extraction of Bioactive Compounds from Olea europaea Leaves. Biomass 2024, 4, 647-657.
Vardakas, A.; Kechagias, A.; Penov, N.; Giannakas, A.E. Optimization of Enzymatic Assisted Extraction of Bioactive Compounds from Olea europaea Leaves. Biomass2024, 4, 647-657.
Vardakas, A.; Kechagias, A.; Penov, N.; Giannakas, A.E. Optimization of Enzymatic Assisted Extraction of Bioactive Compounds from Olea europaea Leaves. Biomass 2024, 4, 647-657.
Abstract
Nowadays circular economy trend drives researchers in the recovery of various bioactive compounds from agri-food by-products. Enzyme-assisted extraction (EAE) is shown as an innovative green technology for the effective extraction of various phytochemicals from agri-food section by-products, therefore this study aimed to evaluate the application of EAE as green technology to obtain extracts from olive leaves (Olea europaea) for potential industrial production. EAE was conducted under various enzyme dose combinations, and incubation time (120 min). Obtained extracts were characterized in terms of total polyphenols (TP) and Total Antioxidant Activity (AA). Firstly, the enzyme synergistic effect in enzymatic extraction of polyphenols was evaluated. TP optimal extraction conditions (468.19 mg GAE/L) were achieved after EAE (50-50%) and for AA (69.85 AA%). According to the above results, a second experimental part occurred for the incubation time (min.) and enzyme dose (mL) of optimal extraction conditions of olive leaves. Results indicated EAE as an excellent choice for green extraction of polyphenols from the olive leaves.
Keywords
plant by-product, antioxidants, green technology, enzyme preparation, polyphenols
Subject
Environmental and Earth Sciences, Sustainable Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
