Diagnostic Potential of miR-143-5p, miR-143-3p, miR-551b-5p and miR-574-3p in Chemoresistance of Locally Advanced Gastric Cancer: A Preliminary Study

Marlena Janiczek-Polewska; Tomasz Kolenda; Paulina Poter; Joanna Kozłowska-Masłoń; Inga Jagiełło; Katarzyna Regulska; Julian Malicki; Andrzej Marszałek

doi:10.20944/preprints202406.0579.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 9 June 2024 / Approved: 10 June 2024 / Online: 11 June 2024 (08:28:32 CEST)

Introduction: Gastric cancer (GC) is one of the most frequently diagnosed cancers in the world. Although the incidence is decreasing in developed countries, treatment results are still unsatisfactory. The standard treatment for locally advanced gastric cancer (LAGC) is gastrectomy with perioperative chemotherapy. Aim: Assessment of selected microRNAs (miRNAs) in chemoresistance in archival material from LAGC. Material and method: The research group consisted of archival material from 10 patients with LAGC. Histological material from each patient was used from a biopsy performed during gastroscopy and after surgery preceded by 4 cycles of neoadjuvant chemotherapy (NAC) according to the FLOT or FLO regimen. The expression of selected miRNAs was assessed in the tissue material, such as miRNA-21-3p, miRNA-21-5p, miRNA-106a-5p, miRNA-122-3p, miRNA-122-5p, miRNA-143-3p, miRNA-143-5p, miRNA-203a-3p, miRNA-203-5p, miRNA-551b-3p, miRNA-551b-5p and miRNA-574-3p. miRNA expression was assessed by quantitative chain reaction polymerase reverse transcriptase (qRT-PCR). The response to NAC was assessed by computed tomography of the abdomen, chest and histopathology after gastrectomy. The statistical analyses were performed using GraphPad Prism 9.The significance limit was set at p

microRNAs; locally advanced gastric cancer; pathological staging; clinical staging; neoadjuvant chemotherapy

Biology and Life Sciences, Biology and Biotechnology

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Diagnostic Potential of miR-143-5p, miR-143-3p, miR-551b-5p and miR-574-3p in Chemoresistance of Locally Advanced Gastric Cancer: A Preliminary Study

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