PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Identification and Differential Expression Analysis of miRNAs in Lirianthedelavayi Reveals Lacks of miRNA Expression in Plant-Pathogen Interaction Pathway in the Cultivated Population
Version 1
: Received: 24 June 2024 / Approved: 24 June 2024 / Online: 25 June 2024 (10:09:49 CEST)
How to cite:
Zhao, T.; Yang, J.; Yuan, L.; Luo, Y.; Zhang, P.; Xu, T. Identification and Differential Expression Analysis of miRNAs in Lirianthedelavayi Reveals Lacks of miRNA Expression in Plant-Pathogen Interaction Pathway in the Cultivated Population. Preprints2024, 2024061744. https://doi.org/10.20944/preprints202406.1744.v1
Zhao, T.; Yang, J.; Yuan, L.; Luo, Y.; Zhang, P.; Xu, T. Identification and Differential Expression Analysis of miRNAs in Lirianthe delavayi Reveals Lacks of miRNA Expression in Plant-Pathogen Interaction Pathway in the Cultivated Population. Preprints 2024, 2024061744. https://doi.org/10.20944/preprints202406.1744.v1
Zhao, T.; Yang, J.; Yuan, L.; Luo, Y.; Zhang, P.; Xu, T. Identification and Differential Expression Analysis of miRNAs in Lirianthedelavayi Reveals Lacks of miRNA Expression in Plant-Pathogen Interaction Pathway in the Cultivated Population. Preprints2024, 2024061744. https://doi.org/10.20944/preprints202406.1744.v1
APA Style
Zhao, T., Yang, J., Yuan, L., Luo, Y., Zhang, P., & Xu, T. (2024). Identification and Differential Expression Analysis of miRNAs in <em>Lirianthe</em> <em>delavayi</em> Reveals Lacks of miRNA Expression in Plant-Pathogen Interaction Pathway in the Cultivated Population. Preprints. https://doi.org/10.20944/preprints202406.1744.v1
Chicago/Turabian Style
Zhao, T., Pinzheng Zhang and Tao Xu. 2024 "Identification and Differential Expression Analysis of miRNAs in <em>Lirianthe</em> <em>delavayi</em> Reveals Lacks of miRNA Expression in Plant-Pathogen Interaction Pathway in the Cultivated Population" Preprints. https://doi.org/10.20944/preprints202406.1744.v1
Abstract
Lirianthe delavayi has valuable ornamental and medicinal value, is an important economic tree, and has a long history of cultivation. As important regulatory molecules, miRNAs are involved in important physiological processes, such as the plant stress response, organ morphogenesis, hormone secretion, and signaling. In this study, based on miRNA sequencing analysis of wild and cultivated Lirianthe delavayi, we explored the differences in posttranscriptional regulation during the cultivation of Lirianthe delavayi from the aspect of miRNA molecular regulation. The results revealed the following: 1. A total of 365 miRNAs were sequenced and identified, and their first bases had obvious uracil (U) base preferences. Among them, 242 miRNAs were classified into 32 miRNA families; 2. Analysis of miRNA expression differences revealed that 39 miRNAs had significant expression differences between wild and cultivated populations; 3. Bioinformatics target gene prediction and target gene enrichment analysis of significantly differentially expressed miRNAs revealed that the target genes were significantly enriched in plant‒pathogen interactions, sphingolipid metabolism, defense responses, and ADP binding. 4. The plant‒pathogen interaction pathway involves seven differentially expressed miRNAs and 30 miRNA‒target gene regulatory pairs, which are involved mainly in pathogen signature molecule recognition and binding, signal transduction, and the regulation of disease resistance molecules or gene expression; Ath-MIR169g-p3_2ss7AG19GA, ath-MIR169g-p5_2ss7AG19GA, and ath-miR408-3p_L-3 are expressed only in wild population, and it is hypothesized that the domestication process from wild to cultivation species may be accompanied by the loss of disease resistance-related miRNAs. This study provides a reference for exploring the posttranscriptional-level regulatory mechanisms of adaptive changes in Lirianthe delavayi from wild to cultivated.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
