MS-BA400-v3 ENG

ENGLISH
Service manual
Manual version Revision date Changes
3.0 November 2015 Modification of chapters: 3.3, 3.7, 3.10,
8, AI
Inclusion of new chapters AII and AIII
2.0 October 2014 Modification of chapters: 3.9, 5, 7.1, AI
and AIV
Inclusion of new chapter 9
1.0 June 2012 Initial version
Manual code TESE000012-03-ESP
Manufacturer's address BIOSYSTEMS

c/Costa Brava 30,
08030 Barcelona
SPAIN
http://www.biosystems.es
The BA400 is compliant with EU directive 98/79/EC

TABLE OF CONTENTS
Persons for whom this manual is intended...................................................................10
Notices and warnings...................................................................................................10
Software usage licence..................................................................................................15
Foreseen use.................................................................................................................16
1. Introduction................................................................................................ 16
2. General description of the analyser................................................................ 17
2.1. Sample rotor................................................................................................................. 17
2.2. Reagent rotor................................................................................................................ 17
2.3. Reaction rotor............................................................................................................... 18
2.4. Optical system ............................................................................................................. 18
2.5. Wash station................................................................................................................. 18
2.6. Waste, distilled water and washing solution containers........................................................ 19
2.7. Stirring arm.................................................................................................................. 19
2.8. Dispensing arm . .......................................................................................................... 19
2.9. ISE module (optional)................................................................................................... 20
2.10. Operating mode............................................................................................................ 20
3. Mechanical elements..................................................................................... 21
3.1. Sample rotor................................................................................................................. 21
3.2. Reagent rotor................................................................................................................ 23
3.3. New barcode reader support design.................................................................................. 24
3.3.1. Reagent rotor reader adjustment............................................................................. 27
3.3.2. Sample rotor reader adjustment............................................................................... 30
3.4. Reaction rotor............................................................................................................... 32
3.5. Pipetting arms.............................................................................................................. 37
3.6. Stirring arm.................................................................................................................. 38
3.7. Dispensation pump assembly.......................................................................................... 39
3.7.1. Dispensation pump assembly.................................................................................. 39
3.7.2. New dispensation pump assembly design................................................................. 40
3.8. Structure ..................................................................................................................... 44
3.9. Fluid connections.......................................................................................................... 45
3.9.1. Modification of the fluidic connections.................................................................... 47
3.10. Degasser...................................................................................................................... 49
4. Electronic elements....................................................................................... 50
4.1. Sources and input-AC board - CIIM00056....................................................................... 51
4.2. Distribution board - CIIM00047.................................................................................... 52
4.3. Main board (CPU) - CIIM00046.................................................................................... 55
4.4. Arm boards - CIIM00048.............................................................................................. 58
4.5. Tip detection board - CIIM00049................................................................................... 60
4.5.1. Level detection adjustment..................................................................................... 62
4.6. Photometric control board - CIIM00050......................................................................... 63
4.7. Photometric readings board - CIIM00051........................................................................ 66
4.8. Rotor boards - CIIM00052............................................................................................ 67
4.9. Fluid control board – CIIM00053................................................................................... 71
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Service manual
4.10. Syringe control board - CIIM00054................................................................................ 75

4.11. Loading of firmware...................................................................................................... 79
4.12. Electronic element specifications..................................................................................... 80
5. Fluidic elements........................................................................................... 81
6. Service programme....................................................................................... 84
6.1. Identification of the programme parts.............................................................................. 84
6.1.1. List of common icons............................................................................................ 85
6.1.2. Vertical bar icons list............................................................................................. 85
6.1.3. Explanation of the adjustment text box.................................................................... 85
6.1.4. Description of the status icons................................................................................ 86
6.2. Programme initialisation................................................................................................ 87
6.3. Description of the menus............................................................................................... 87
6.4. Setup........................................................................................................................... 88
6.4.1. Analyser............................................................................................................... 88
6.4.2. Communications.................................................................................................. 89
6.4.3. Language............................................................................................................. 90
6.4.4. Barcode............................................................................................................... 90
6.4.5. Users................................................................................................................... 90
6.5. Adjustments/Test.......................................................................................................... 91
6.5.1. Adjust positioning................................................................................................. 91
6.5.1.1. Optical centring adjustment.........................................................................................................91
6.5.1.2. Wash station adjustment..............................................................................................................92
6.5.1.3. Adjusting the arm positioning.......................................................................................................93
6.5.2. Photometry.......................................................................................................... 95
6.5.2.1. Baseline and darkness current.......................................................................................................95
6.5.2.2. Metrology....................................................................................................................................97
6.5.3. Scales, bottles and tanks......................................................................................... 98
6.5.3.1. Scale adjustment for determining level..........................................................................................98
6.5.4. Verification of the dispensation pumps, pumps and valves........................................... 99
6.5.5. Adjusting the thermostatting systems..................................................................... 100
6.5.5.1. Wash station thermostatting adjustment......................................................................................100
6.5.5.2. Adjusting the reaction rotor thermostatting..................................................................................101
6.5.5.3. Adjusting the tip thermostatting..................................................................................................102
6.5.6. Barcode reader adjustment................................................................................... 103
6.5.7. ISE module........................................................................................................ 104
6.5.8. Stress................................................................................................................. 105
6.6. Utilities...................................................................................................................... 106
6.6.1. Demo mode....................................................................................................... 106
6.6.2. Analyser information........................................................................................... 107
6.7. Recording and maintenance.......................................................................................... 108
6.7.1. Historical reports................................................................................................ 108
6.7.2. TAS report......................................................................................................... 108
6.8. Exit........................................................................................................................... 109
7. Maintenance and cleaning........................................................................... 110
7.1. Maintenance activities and frequency..............................................................................110
7.2. Cleaning.....................................................................................................................111
7.2.1. Cleaning the interior compartments.......................................................................111
7.2.2. Checking the fluidic connections...........................................................................111
7.2.3. Cleaning the water and low contamination waste containers......................................111
6
7.2.4. Washing the outside of the tips..............................................................................112
7.2.5. Cleaning the barcode reader window......................................................................112
7.2.6. Wash the heating canal and the canal of the rotor containers......................................112
7.3. Maintenance................................................................................................................112
7.3.1. Checking of the wash station piston pump..............................................................113
7.3.2. Checking of the reagent and sample arms, stirrers and wash station.............................113
7.3.3. Checking the sample and reagent rotors..................................................................113
7.3.4. Inspect the cover and the structure.........................................................................114
7.3.5. ISE module inspection..........................................................................................114
Installation registration sheet....................................................................................................115
Installation registration sheet....................................................................................................116
Preventive maintenance record.................................................................................................117
8. Description of how to dismantle subassemblies and spares............................ 119
8.1. Dismantling of housing.................................................................................................119
8.1.1. Removing the rear cover........................................................................................119
8.1.2. Removing the top cover........................................................................................119
8.1.3. Removing the side housings.................................................................................. 120
8.1.4. Dismantling and assembling the ISE cover............................................................. 121
8.1.5. Remove the top front housing............................................................................... 123
8.1.6. Dismantling the top tray...................................................................................... 123
8.2. Removing the sample and reagent arms and the stirrers..................................................... 124
8.3. Dismantling the reagent rotor....................................................................................... 124
8.4. Dismantling the sample rotor........................................................................................ 125
8.5. Dismantling the reaction rotor...................................................................................... 126
8.6. ISE module installation................................................................................................ 127
8.7. Wash station pump spares............................................................................................ 131
8.7.1. AC16587 - Top chamber and AC16585 - Stop and O-ring....................................... 131
8.7.2. AC16816 - Piston support assembly and pistons..................................................... 132
8.7.3. AC16817 - Clamping device................................................................................ 134
8.7.4. AC16586 - Pump Motor..................................................................................... 134
8.8. Replacing the degasser.................................................................................................. 135
8.8.1. AC17094 - Installing the degasser module.............................................................. 135
8.8.2. Inserting and removing the tubes from the quick connectors..................................... 137
8.8.3. AC17092 - Replacing the degasser membrane......................................................... 137
8.8.4. Inserting and removing the tubes from the quick connectors..................................... 137
8.8.5. AC17220 - Replacing the degasser control board..................................................... 139
8.8.6. Description of how to programme the vacuum switch.............................................. 139
8.9. Replacing the arm....................................................................................................... 141
8.9.1. AC16538 - Arm cover......................................................................................... 141
8.9.2. AC16558 - Tip board.......................................................................................... 141
8.9.3. AC16603 - Sample tip / AC16604 - Reagent tip..................................................... 142
8.9.4. AC16557 - Arm board......................................................................................... 145
8.9.5. AC16599 - Polar belt / AC16600 - Polar motor...................................................... 146
8.9.6. AC16599 - Vertical belt / AC16600 - Vertical motor............................................... 147
8.9.7. AC16601 - Stirrer motor/ AC16602 - Stirrer paddle................................................ 148
8.10. Fluidic assembly replacement........................................................................................ 149
8.10.1. AC16563 - Fluid board / AC16564 - Syringe board................................................. 150
8.10.2. AC16577 - Wash station heater............................................................................. 150
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Service manual
8.10.3. AC16578 - Wash station electro valve.................................................................... 152

8.10.4. AC16579 - Pre-dose manifold.............................................................................. 153
8.10.5. AC16581 - Dispensation membrane pump / AC16582 - Suction pump..................... 153
8.10.6. AC16580 - Inlet electro valve............................................................................... 154
8.10.7. AC16576 - Collector........................................................................................... 155
8.11. Sample and reagent rotor replacement............................................................................ 156
8.11.1. AC16538 - Sample rotor board / AC16562 - Reagent rotor board............................. 156
8.11.2. AC16628 - Barcode reader window and heater........................................................ 157
8.11.3. AC16625 - Barcode reader................................................................................... 157
8.11.4. AC16630 - Reagent rotor cover sensor/ AC16657 - Sample rotor cover sensor............. 158
8.11.5. AC16631 - Temperature sensor............................................................................. 158
8.11.6. AC16626 - Refrigerator fan.................................................................................. 159
8.11.7. AC16627 - Fridge Peltier..................................................................................... 160
8.11.8. AC16632 - Reagent rotor centring device / AC16813 - Sample rotor centring device... 161
8.11.9. AC16623 - Rotor motor / AC17239 - Rotor motor / AC16624 - Belt........................ 162
8.12. Ceramic pump spares................................................................................................... 165
8.12.1. AC16565 - Start-up sensor board / AC16566 - Manifold led board........................... 165
8.12.2. AC17255 - Reagent manifold / AC17254 - Sample manifold.................................... 166
8.12.3. AC16590 - Reagent piston stop / AC16591 - Sample piston stop.............................. 170
8.12.4. AC16593 - Ceramic sample piston /AC16594 - Ceramic reagent piston /AC16592 - Pump
motor + spindle................................................................................................................ 170
8.13. Reaction rotor spares................................................................................................... 172
8.13.1. AC16617 - Rotor centring device.......................................................................... 173
8.13.2. AC17216 - Cover sensor ..................................................................................... 174
8.13.3. AC16620 - Temperature sensor............................................................................. 175
8.13.4. AC17240 - Wash station collision sensor................................................................ 175
8.13.5. AC16597 - Wash station tip spring........................................................................ 176
8.13.6. AC16595 - Wash station suction tip / AC16596 - Suction tip + wash station dryer...... 177
8.13.7. AC16565 - Photobarrier board............................................................................. 178
8.13.8. AC16618 - Wash station elevation motor / AC16619 - Wash station vertical belt......... 179
8.13.9. AC16575 - Reaction rotor support column............................................................ 180
8.13.10. AC16615 - Reaction rotor belt / AC16616 - Reaction rotor motor............................ 181
8.13.11. AC16621 - Rotor thermostatting fan assembly........................................................ 182
8.13.12. AC16622 - Reaction rotor peltier assembly............................................................. 182
8.13.13. AC16559 - Photometric control board - CIIM0050................................................ 183
8.13.14. AC16560 - Photometric board - CIIM0051........................................................... 184
8.13.15. AC16614 - Optical bench assembly without filters / Changing the filters.................... 188
9. Troubleshooting......................................................................................... 190
9.1. Software and firmware installation process...................................................................... 190
9.1.1. Backup copy before executing the version update.................................................... 190
9.1.2. Updating the version........................................................................................... 190
9.1.3. Patch update...................................................................................................... 191
9.1.4. Revert to a previous version.................................................................................. 191
9.1.5. Solving problems related to installation.................................................................. 191
9.1.6. Solution............................................................................................................. 192
9.1.7. Solving of problems related to the computer configuration....................................... 192
9.1.8. Problems in installing the SQL Server.................................................................... 194
9.2. ISE module maintenance.............................................................................................. 196
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9.2.1. Changing the electrodes....................................................................................... 196
9.2.2. Check that the fluid system is operating correctly. . ................................................. 196
9.2.3. Pass a nylon cord through the opening................................................................... 196
9.2.4. Clean the probes and metal contacts of the electrodes and the ISE module with a ...............
cotton bud....................................................................................................................... 197
9.2.5. Wipe the cup to remove all sample traces............................................................... 197
9.2.6. Calibration acceptance margins............................................................................. 197
9.2.7. Noise and drift errors........................................................................................... 197
AI. List of accessories and spares.............................................................................199
AII. Hardware and software modifications...............................................................221
AIII. List of firmware errors.......................................................................................223
AIV. Technical characteristics....................................................................................226
AV. Adjustment margin tables.................................................................................229
AVI. Repackaging instructions..................................................................................231
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Service manual
Persons for whom this manual is intended

This manual is intended for professionals belonging to the technical service who perform preventive maintenance
tasks on and repair the BA400 analyser. These professionals will have received special training enabling them to
perform the above-described tasks.
This manual describes the mechanical and electronic characteristics and service software to assist technicians in
performing maintenance and repair work. It also describes the steps for disassembling and changing the different
elements that comprise the analyser.
Notices and warnings

Explanation of the safety symbols located on the analyser or in this manual.
Symbol Description
The symbol warns of operating risks that could cause personal injury.
WARNING
The symbol warns of a potential biological hazard.
BIOHAZARD
The symbols warns of potential damage to the system or unreliable results.
CAUTION
The symbol warns that the information requires your attention.
NOTE
Risk of electric shock.
The symbol warns of a potential risk due to laser radiation emission.
10
Explanations of the symbols used on the analyser labels and in the manual
Symbol Description
This product is compliant with EC directive 98/79 on medical devices for In
Vitro Diagnostics.
Medical device for In Vitro Diagnostics.
Please consult the directions for use.
Serial number
Expiry date
Batch code
Catalogue number
Temperature limit
Manufacturer
Irritant
Safety precautions
Symbol Description
Preventing electric shock
To prevent the risk of electrocution. Do not remove any of the analyser
housing elements. No user intervention makes it necessary to access the parts
inside the equipment. If necessary, contact the technical assistance service.
Preventing biological risks in handling the samples
Inappropriate handling of samples, controls and calibrators could cause
biological infection. Do not touch the samples, mixtures or waste with your
hands. Wear gloves and protective clothing when necessary.
BIOHAZARD In the event that the samples come into contact with the skin, wash
immediately with abundant water and seek medical advice. It is advisable to
follow good laboratory practices.
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Service manual
Symbol Description
Prevention in handling reagents
Handle reagents and washing solutions with care, they contain substances that
could be corrosive.
In the event that the reagents or washing solutions come into contact with the
WARNING skin, wash immediately with abundant water and in the event of a reaction,
seek medical advice.
Consult the reagent or washing solution adaptation sheet and follow the
safety instructions.
It is advisable to follow good laboratory practices.
Preventing biological risks in handling liquid waste
Handle the high contamination waste container with care. Wear gloves and
protective clothing when handling the container.
Dispose of the waste in accordance with national or local legislation for
BIOHAZARD disposing of dangerous biological waste, and consult the reagent manufacturer
or distributor for more details.
Preventing biological risks in handling solid waste
Take care in handling parts of the analyser that are converted to waste such as
the reactor rotor, sample tubes and reagent bottles.
Wear gloves and protective clothing when handling such waste.
BIOHAZARD Dispose of the waste in accordance with national or local legislation for
disposing of dangerous biological waste, and consult the reagent manufacturer
or distributor for more details.
Prevention of electro magnetic interferences
The analyser complies with the emission and immunity requirements set out
in standard UNE-EN 61326-2-6:2006. This equipment has been designed
and tested for class B of standard UNE-EN 55022:2000. In a domestic
NOTE environment, it may cause radio interference, in which case it may be
necessary to take measures to mitigate the interference.
Do not use the analyser near strong electro magnetic radiation sources (such
as centrifuge appliances, radio transmitters, mobile telephones), as they could
interfere with its correct operation.
Preventing laser light emission risks
The analyser has two barcode readers that emit laser light. The readers only
function when the analyser is in execution mode and its rotor covers are in
place. In the event of a failure or during adjustment by technical maintenance
staff, the light beam could be activated without the cover in place; in such
cases, do not look directly at the laser beam.
Prevention at the end of the useful life of the analyser
At the end of the useful life of the analyser, the product must be disposed of
in accordance with the environmental legislation in force in each country.
If that country is a EU member state, the terms of the WEEE directive
on electrical and electronic appliances will apply. In other words, when
the appliance's useful life has ended, it is converted into waste and must
be separated from household waste for correct recycling. For this purpose,
contact the distributor for the product to be properly recycled.
Abbreviations and units shown in the manual
12
Abbreviation Definition
∅ Diameter
EC European Community
EMC Electromagnetic compatibility
CRTL Control key on the computer keyboard
EN European norm
F Fast (fuse type)
FUS Fuse
ISE Ion-selective electrode
IVD In Vitro Diagnostics
LED Light-emitting diode
LIS Laboratory information system
WEEE Waste Electrical and Electronic Equipment
REF Reference solution for the ISE unit
TAS Technical assistance service
SD Standard deviation
ES Electrical safety
UV Ultraviolet
Units Definition
" Inch
˚C Degrees centigrade
A Ampere / Absorbance
GB Gigabyte
h Hour
Hz Hertz
kg Kilogram
L Liter
MB Megabyte
m Meter
min Minute
mL Milliliter
mm Millimeter
mmol Millimole
mV Millivolt
nm Nanometer
Nm Newton Meter
prep Preparation
s Second
VA Volt-ampere
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Service manual
Units Definition
V Volt
W Watt
μL Microliter
μm Micrometer
14
Software usage licence
BIOSYSTEMS, the exclusive owner of all the rights over this computer application, grants one non-transferable,
non-exclusive licence governing the use of the computer application to the user, who accepts it solely and exclusively
for executing this computer application in a single central processing unit (CPU) of a computer.
This licence does not permit the execution, use, access, reproduction, transformation, translation, lease, sale, distri-
bution, commercial exploitation or provision to third parties in any way, and above all in a computer network or
through remote access technologies, of all or part of the content included in this DVD.
BIOSYSTEMS will in no case be liable or assume payment of any compensation whatsoever:
with respect to any infringement of the intellectual and/or industrial property rights of others caused by copying
images, audio and/or text as part of the content of this DVD;
due to the exhaustiveness or accuracy of the data incorporated through incorrect use of the computer application
in the DVD;
for damages, losses or indirect, special, incidental or consequential damages to people or property due to the incor-
rect use or putting into practice of any of the methods, theories, products, instructions, ideas or recommendations
included in this DVD or which make reference to its content.
The computer application in this DVD is delivered with no guarantee of the results obtained following incorrect use
or adaptation for a specific purpose. The user fully assumes all risks with regard to the results obtained by incorrect
use of the computer application.
None of the contents of this usage licence grants the user any intellectual or industrial property rights or rights
over the confidential information of BIOSYSTEMS and/or of persons with rights over the content of this DVD.
The licence granted and constituted pursuant to these terms and conditions will be interpreted in accordance with
and governed by Spanish law, with the jurisdiction being the courts of the city of Barcelona, Spain. The user waives
any other applicable legislation and/or jurisdiction that is competent, if any.
The user of this licence knows and agrees that the user licence grants no rights over the use of computer programmes
and/or applications belonging to others that may be used or which may be necessary for using or operating this
computer application, for which the user will obtain the respective legitimisation of use.
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Service manual
Foreseen use
The BA400 analyser is used to determine analyte concentrations by in vitro biochemical, turbidimetric and electrolyte
measurements of human samples of serum, urine, plasma, cerebrospinal fluid or total blood.
The BA400 analyser has been optimised to operate with the BioSystems biochemistry, turbidimetric and electrolyte
reagent lines. Reagents not included in the BA400 analyser validation performed in BioSystems SA require a com-
plete and detailed validation by the user or the laboratory.
Users are strongly advised to validate the overall functioning of the analyser and of the reagents in the laboratory,
taking into consideration the preanalytical phase and all other relevant aspects.
The analyser is exclusively for professional use, i.e., for users who have the appropriate training and expertise to use
it. In addition to how to install the instrument, users are instructed on how to use the analyser and the software
that goes with it.
The environmental conditions for the functioning of the analyser are normal clinical analysis laboratory conditions.
These conditions are set out in the specifications chapter.
1. Introduction
The BA400 analyser is an automated, random access analyser that takes readings at 400 prep/h. It has been specially
designed to perform clinical biochemical and turbidimetric analyses and take electrolyte readings. Control of the
instrument is performed on-line in real time from a dedicated external PC.
The analyser has 5 arms: 2 arms for handling reagents, 1 arm for handling samples and 2 arms for stirring the reagent
and sample mixture.
There are two rotors, with the reagents being positioned in one and the samples in the other.
The reagent rotor is refrigerated and both rotors have barcode readers.
The reagent and sample are mixed in the third rotor or reaction rotor. It is also where the photometric readings
are taken while the reaction develops. The reaction rotor is thermostatted and has a wash station for emptying the
completed reactions and for washing and drying the cuvettes for the next preparation round. It therefore processes
a continuous flow of preparations.
To ensure safety, the analyser has the following elements: vertical arm collision detector, sample tip clot detector
and detectors in all the covers.
16
2. General description of the analyser
In mechanical terms, the analyser is divided up into subassemblies. Each subassembly has its own electronic board
for controlling the individual subassembly elements.
All the subassemblies are electrically connected by the CAN bus cable. The CAN bus cable contains the power wires
and the wires that transmit information between the boards.
A list of the subassemblies is given below:
• Sample rotor
• Reagent rotor
• Reaction rotor
• Pipetting arm
• Stirring arm
• Wash station
• Dispensing pumps
• Fluidic system
• Washing solution and high contamination waste bottles
• Electrical and communication connections
• ISE module (optional)
2.1. Sample rotor

The sample rotor is comprised of a drum for positioning the samples, a cover and a barcode reader.
The drum has a circular structure with 3 concentric rings in which the tubes or sample wells are placed.
Each of the 3 rings has 45 positions for tubes with diameters of between 12 and 16 mm and heights of up to 100 mm
The barcode reader can only read the primary tubes with codes located in the two outer rings.
The accessories box has some adapters for insertion in each of the positions in the event that they must contain
sample wells instead of primary tubes.
The positioning of the serum in the rings is not really relevant but the following positioning is recommended:
Calibration serums, controls and special solutions (such as washing and dilution solutions) should be placed in the
innermost ring, in sample wells. Samples should be placed in the two outer rings in primary tubes so that they can
be read by the barcode reader.
2.2. Reagent rotor

The reagent rotor is comprised of a drum for positioning the samples, a cover and a barcode reader. The whole as-
sembly is refrigerated.
The drum has a circular structure with 2 concentric rings with positions for inserting the reagent bottles.
Each ring has 44 positions.
The barcode reader can read the codes of the bottles positioned in both rings.
There are 2 types of bottle: some have a capacity of 60 mL and others have a capacity of 20 mL.
The 20 mL bottles can only be placed in the outer ring.
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Service manual
Both 60 mL and 20 mL bottles can be placed in the inner ring.

The rotor is refrigerated, and the mean temperature inside it is below 8 ºC. The fridge has an independent power
supply system with its own switch so that when the analyser is switched off, the fridge can continue to operate.
2.3. Reaction rotor

The reaction rotor has 120 positions and a cover. The whole assembly is refrigerated.
The rotor is a single part made of PMMA and has 120 positions. The sample and reagent mixture reactions are
performed on it. Different optical readings are taken during the reaction. The PMMA material filters UV radiation.
The rotor is maintained at a stable temperature of 37 ºC by a Peltier-based thermostatting system.
The reaction volumes range is from 180 μL to 600 μL.
Each arm dispenses in a different position from the rotor. The dispensing positions are:
• Cycle 1: R1 dispensation
• Cycle 31: S (sample) dispensation
• Cycle 32: R1+S stirring
• Cycle 33: Initiation of photometric readings
• Cycle 66: R2 dispensation and stirring of reagent 2
• Cycle 100: End of the reading processes
• Cycles 101 –111: Elimination of the completed reaction and cleaning of the sample well in the wash station.
2.4. Optical system

The optical system is located in the reaction rotor, below the wash station.
It is formed by a set of leds, filters, beam splitters, the reaction rotor and two photodiodes.
There is one principal reading diode and a reference photodiode that enables the correction of perturbations gener-
ated in the light source.
The analyser has 8 wavelengths: 340, 405, 505, 535, 560, 600, 635, 670.
The measuring range is from -0.2 Abs to 3.5 Abs.
The measuring resolution is 0.0001 Abs.
The system automatically performs a blank on the cuvette before dispensing the reagent. This blank absorbance
value is used to correct the absorbance values measured in the cuvette.
If this value exceeds a preestablished limit, the cuvette is discarded.
2.5. Wash station

The wash station consists of an assembly with 7 tips located above the reaction rotor.
Each tip has a specific function and corresponds to a different execution cycle:
• Cycle 1: Suction of the high contamination waste and dispensing of the washing solution
• Cycle 2: Suctioning and dispensing of the washing solution
• Cycle 3: Washing solution rest phase
18
• Cycle 4: Suctioning of the washing solution and dispensing of distilled water
• Cycles 5 and 6: Suctioning and dispensing of distilled water
• Cycle 7: Distilled water rest phase
• Cycle 8: Optical scanning of cuvette
• Cycle 9: Suctioning of distilled water
• Cycle 10: Drying of cuvette
The distilled water for rinsing and the washing solution are thermostatted so that they do not interfere with the
rotor temperature.
When the last rinse is made, an optical reading is taken of the rotor sample well, in order to verify the state of the
well. If it is scratched or in poor condition, the well is discarded and not used for performing reactions.
In the event of there being a large number of wells in poor condition, the programme warns that the reaction rotor
needs to be replaced.
2.6. Waste, distilled water and washing solution containers

The analyser has 4 containers for storing waste, distilled water and washing solution. These containers are located
inside it.
The high contamination waste and washing solutions containers can be accessed from the front.
The capacity of both containers is 5 L. This capacity allows for 8 hours of continuous autonomous operation.
Detection of whether the bottle is full or empty is done by weighing.
The low contamination waste and distilled water containers are located inside the analyser, at the rear, and cannot
be accessed by the user. These containers have a buoy system that informs the analyser when they are full or empty.
They are automatically filled and emptied from the exterior.
The external distilled water inlet may come from a pressurised distilled water inlet or an external tank with a larger
capacity.
The low contamination waste leaves the appliance through a drainage pipe that goes directly to a tank or sump.
2.7. Stirring arm

The analyser has two arms for stirring the reaction. These arms have a small blade that rotates inside the reaction
cuvettes, to ensure the proper mixing of the reagent and sample.
One of the stirring arms is inserted into the cuvette after dispensing the sample and the other stirring arm is inserted
after the second reagent is dispensed in reactions with two reagents.
After mixing, the arm is washed in the wash station provided for that purpose.
2.8. Dispensing arm

The analyser has 3 arms for dispensing samples and reagents.
One arm is for dispensing serum and urine samples and the other two are for dispensing the reagents. One is used
to dispense reagent 1 and the other to dispense reagent 2, only in bireactive reactions.
Each arm has a wash station for washing the interior and exterior of the tip.
The volumes dispensed by each arm are the following:
• Sample arm: from 2 μL to 40 μL
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Service manual
• Reagent arm R1: from 90 μL to 450 μL

• Reagent arm R2: from 10 μL to 300 μL
Each arm has a tip with an automatic level detection system. The tip descends until it reaches the reagent or sample,
depending on the case, and then suctions the programmed volume. This prevents the tip from penetrating too deeply
in the fluid and makes it easier to wash the tip.
Each arm has a vertical collision detection system to prevent the tips from colliding with each other and ensure
they are not damaged.
The sample dispensing arm has a clot detector. This system warns the user if the tip becomes blocked when suction-
ing the sample. The blockage may be due to traces of fibrin or clotting present in the serum sample.
2.9. ISE module (optional)

The ISE ion module is optional, and is used to determine Na+, K+, Cl- and Li+ ions in serum and urine samples.
The ion module is fully autonomous and functions in parallel, together with the biochemical determinations.
When ISE ion testing of patients is programmed in the list, the sample-dispensing arm is responsible for positioning
the sample in the ion module.
The module has a fully sealed kit that contains the calibration standards and collects the waste. This kit is an acces-
sory and its compartment is accessible from the front part of the analyser.
2.10. Operating mode

The analyser performs the tests patient by patient and permits the continuous input of samples. The analyser is
controlled by a dedicated PC that is in permanent communication with the instrument. The application programme
installed in the computer keeps the user constantly informed about the state of the analyser and the progress of the
tests. As the results are obtained, the computer immediately displays them to the user.
When starting a Worksession, the analyser proposes the performing of the blanks, calibration standards and controls
programmed for the measuring procedures it must execute. The user can decide whether or not to execute the blanks
and calibration standards. If they are not executed, the analyser will use the latest data available. The controls can also
be activated or not activated. During a worksession, while the analyser is operating, the user can enter new routine
or urgent samples for analysis. Every time a new sample is added, the analyser automatically proposes potential new
blanks, calibration standards or controls that must be executed. It is advisable to restart the session each working day.
The analyser determines the concentrations of the analytes based on optical absorbance measurements. To measure
the concentration of an analyte in a sample, the analyser pipettes a specific volume of reagent, thermostats it in the
same tip and dispenses it into the reaction rotor.
After 5 minutes the analyser pipettes a specific sample volume and dispenses it into the same well in which the
reagent was dispensed.
During the next cycle the stirring mixes the reagent to ensure correct homogenisation and the chemical reaction
is initiated. In the bireactive modes, the reaction is initiated when the analyser dispenses a second reagent into the
same reaction well and it is mixed by a second stirrer.
The reactions may be biochemical or turbidimetric reactions. In both cases, the reaction or chain of reactions
produced generate substances that attenuate certain light wavelengths, either by absorption or by dispersion. By
comparing the luminous intensity of a certain wavelength that penetrates a well when a reaction occurs and when
there is no reaction, it is possible to determine the respective analyte concentration. This comparison is quantified
with the physical magnitude absorbance. In some cases the concentration is a direct function of the absorbance and
in others, it is a function of the change of the absorbance over time, depending on the analysis mode.
20
3. Mechanical elements
In mechanical terms, the analyser has a tubular structure to which the different subassemblies and housing elements
are attached.
The analyser has the following subassemblies:
• Sample rotor
• Reagent rotor
• Reaction rotor
• Pipetting arms
• Stirring arms
• Wash station
• Dosing system
• Fluidic system
• ISE module (optional)
3.1. Sample rotor

The tubes with the patient serum samples and the wells with the calibration standards and controls are placed in the
sample rotor. The rotor (3) has 3 rings. Tubes with larger diameters (16 mm) are placed directly in the holes. Tubes
with smaller diameters or sample wells require the use of adapters (2) to secure them in place.
6
4
Figure 3.1 Sample rotor assembly
The rotor (3) has a single position once it has been inserted into its compartment (4). For this reason it has a cen-
tring device (6) to guide it. Once inserted, the rotor cannot be removed as it is blocked by a ball-shaped anchoring
device (7). To release the rotor from the base it is necessary to press the button (5).
The rotor vessel (9) is secured to the rotor assembly support (10). The rotor (3) is connected to the rotor centring
device, which in turn is connected to the pulley by a shaft (14). The belt (13) transmits the motor movement (11)
to the whole assembly.
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Service manual
The start-up sensor (12) ensures the initial position of the sample rotor by means of a tab on the pulley.
The vessel exterior is protected by a cover (1). A Hall effect sensor (8) indicates that the cover is on the analyser.
References of figures 3.1, 3.2 and 3.3:
1 – Sample rotor cover 13 – Transmission belt
2 – Adapters for tube and sample well 14 – Pulley
3 – Sample rotor 15 – Barcode reader protective window
4 – Rotor assembly 16 – Barcode reader
5 – Rotor anchoring button 17 – CIIM00052 electronic board
6 – Rotor positioning device 18 – Set screw for angular orientation of barcode reader
7 – Rotor ball-shaped anchorages 19 – Set screw for adjusting the barcode reader height
8 – Cover sensor 20 – Set screw for barcode reader proximity adjustment
9 – Rotor vessel
10 – Rotor assembly support
11 – Rotor circular movement motor
12 – Rotor start-up sensor
15
8 16
9
17
10
11
12
13
14
Figure 3.2 Detail of sample rotor assembly
The barcode reader (16) is secured by a support to the subassembly structure. It is positioned at the correct height
for the light to correctly illuminate the tubes placed in the rotor.
There is a protective window to isolate the reader from the exterior.
The barcode reader position can be adjusted using mechanical means.
))
See chapter 6 for applying the service programme and reader adjustment and see Figure 3.3.
• To adjust the angular orientation, loosen the screws (18) and move the reader with your hand until you obtain
a correct reading. Tighten the screws (18).
• To adjust the reader height, loosen the screws (19) and move the reader upward or downward with your hand.
Once the readings are correct, tighten the screws (19).
• To adjust the reader proximity, loosen the screws (20) and move the reader forward or backward with your
hand. Once the readings are correct, tighten the screws (20).
22
18
19
20
Figure 3.3 Barcode reader adjustment
3.2. Reagent rotor

The reagent bottles are placed in the reaction rotor. There are two types of bottle, one containing 60 mL and one
containing 20 mL. The rotor (3) has 2 rings. Only 20 mL bottles can be placed in the outer ring, and both 20 mL
and 60 mL bottles can be placed in the inner ring. The assembly has a barcode reader that reads the codes on the
bottles placed in both rings. The whole assembly is refrigerated and has a separate power supply system to maintain
the refrigeration when the appliance is turned off.
5
4
6
Figure 3.4 Sample rotor assembly
The rotor transmission system and the barcode reader support and adjustment are exactly the same as those of the
sample rotor.
))
See chapter 3.1, description of the transmission system and barcode reader support.
The reagent rotor compartment is refrigerated. To maintain the temperature, the vessel is protected by an insulator
(8). The cooling system is performed by 4 peltiers (10) that cool the vessel through the copper splitters (9). The heat
produced by the peltiers is evacuated through the radiators (11) and fans (12).
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Service manual
References of figures 3.4 and 3.5:

1 – Reaction rotor cover 7 – Rotor ball-shaped anchorages
2 – 60 mL and 20 mL reagent bottles 8 – Insulator
3 – Reagent rotor 9 – Peltier splitter
4 – Rotor assembly 10 – Peltier
5 – Rotor anchoring button 11 – Radiator
6 – Rotor positioning device 12 – Fan
9
10
11
12
Figure 3.5 Reagent rotor cut-off
3.3. New barcode reader support design

A second barcode reader reference has been validated for the reagent rotor and the sample rotor. For this purpose,
it was necessary to modify the reader support parts.
)) See chapter AII to see the serial numbers from which the above was implemented.
These modifications were applied from serial number:
The two barcode reader references are:
• Microscan MS3
• Opticon NLB1000
The different positions of the support plates are given below, depending on the type of reader and the rotor.
Part (figures 3.4 and

Reader type Rotor model Assembly position
3.5)
Part Nº 1 Position 13 (figure 3.8)
Positions 9 and 10 (figure 3.6)
Reagents and Part Nº 2
Microscan Marked MIC
Samples
Add this part to hold the reader in place. Single
Part Nº 6
position (figure 3.4)
Opticon Reagents Position 12 (figure 3.7)
Part Nº 2
Marked M
24
Part (figures 3.4 and
Reader type Rotor model Assembly position
3.5)
Opticon Samples Position 11 (figure 3.7)
Part Nº 2
Marked R
1 2 3 4 5 6 7
Figure 3.6 Exploded view of Microscan reader support
25
Service manual
1 2 3 4 5 8
Figure 3.7 Exploded view of Opticon reader support
9 Microscan Reagents and samples

10 Microscan Reagents and samples
Figure 3.8 Microscan reader assembly. Reagents and samples rotor
26
Opticon Reagents 11
12 Opticon Samples
Figure 3.9 Opticon reader assembly
13 Microscan Reagents and samples. Opticon Reagents
14 Opticon Samples
Figure 3.10 Support plate assembly
References of Figure 3.6, Figure 3.7, Figure 3.8, Figure 3.9 and Figure 3.10:
1 – Unified L support of the reader assembly 10 – Position of Microscan reader reagents and samples
2 – Selection support 11 – Position of Opticon reader reagents and samples
3 – Set screw insulator washer 12 – Position of Opticon reader samples
4 – Insulator separator 13 – Position of Microscan reader support and of Opticon
5 – Part for holding the reader in place reader reagent rotor
6 – Extra part for holding the Microscan reader in place 14 – Position of Opticon reader sample rotor support
7 – Microscan reader
8 – Opticon reader
9 – Position of Microscan reader reagents and samples
3.3.1. Reagent rotor reader adjustment

Start up the analyser with the service programme and select the barcode reader adjustment menu.
1. Adjustments /test / Barcode
2. Select the type of rotor: Reagents
3. Install the reader support angle bracket in the position (1).
4. Place the reader in the mid position of the mounting hole in all 3 adjustments (2), (3) and (4). See Figure 3.11
and Figure 3.12
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Service manual
5. With the service programme, put the analyser in the adjustment mode to switch on the reader laser beam.
Adjust the laser beam so it is as parallel as possible to the rotor shaft using the screws (2) that hold the bracket
in place. Using a set square or tool, check that the beam is parallel. See Figure 3.13.
6. Place a reagent rotor and a bottle in position 1 with a label. Adjust the vertical position angle (4) to ensure the laser
beam covers the whole label and the start of the laser is at the top position of the bottle as shown in Figure 3.14.
7. Adjust the laser angle (3) so that the beam has a deviation of 12 to 14 mm from the centre of the shaft, see Fig-
ure 3.15.
8. With the service programme, adjust the central position of bottle number 1 with the controls and then check
all the rotor readings.
1 2
Figure 3.11 Barcode reader distortion adjustment
28
3 4
Figure 3.12 Barcode reader angular and inclination adjustment
Figure 3.13 Checking the barcode reader adjustment
29
Service manual
Figure 3.14 Checking the laser beam positioning
From 12 mm to 14 mm
Figure 3.15 Checking the laser beam positioning
3.3.2. Sample rotor reader adjustment

Start up the analyser with the service programme and select the barcode reader adjustment menu.
1. Adjustments /test / Barcode
2. Select the type of rotor: Samples
3. Install the reader support angle bracket in the position (5).
4. Place the reader in the mid position of the mounting hole in all 3 adjustments (6), (7) and (8). See Figure
3.16, Figure 3.17 and Figure 3.18
5. With the service programme, put the analyser in the adjustment mode to switch on the reader laser beam.
Adjust the laser beam so it is as parallel as possible to the rotor shaft using the screws (6) that hold the bracket
in place. Using a set square or tool, check that the beam is parallel. See Figure 3.19.
30
6. Place a reagent rotor with a sample tube in position 1 with a barcode label. Adjust the vertical position angle
(8) to ensure the laser beam covers the whole label and the start of the laser is at the top position of the tube
as shown in Figure 3.19.
7. Adjust the laser angle (7) so that the beam is centred with the shaft, see Figure 3.19.
8. With the service programme, adjust position 1 central with a tube using the controls and then check all the
rotor readings.
5 6
Figure 3.16
7
Figure 3.17
31
Service manual
Figure 3.18
Figure 3.19
3.4. Reaction rotor

The reaction rotor is the place where the sample is mixed with the reagent. The rotor (2) has 120 PMMA wells.
The rotor is installed in a heating canal (3) thermostatted at 37 ºC. The entire assembly is protected by a cover (1),
to maintain the temperature inside it and prevent light from entering. The system functions continuously and for
this purpose, it has a wash station (4) which empties and washes the rotor wells in 10 cycles. During each cycle, the
analyser reads 68 wells with the optical system (5) that is built into the assembly.
32
1
3
4
Figure 3.20 Reaction rotor assembly
7
2
10
11
12
13
14
Figure 3.21 Reaction rotor cut-off
33
Service manual
11
12
15
16
17
18
13
14
19
Figure 3.22 Reaction rotor transmission assembly
References of Figure 3.20, Figure 3.21, and Figure 3.22:

1 – Reaction rotor cover 11 – Radiator
2 – Reaction rotor 12 – Fan
3 – Heating canal 13 – Reading encoder
4 – Wash station 14 – Transmission wheel
5 – Optical system 15 – Rotor motor operation
6 – Heating canal insulator 16 – Motor pinion
7 – Rotor anchoring bolt 17 – Transmission belt
8 – Rotor centring device 18 – Encoder photosensor
9 – Rotor start-up sensor 19 – Rotor assembly support column
10 – Peltier
The PMMA rotor (2) is centred by means of tabs with a size that is different to that of the rotor centring device (8)
and secured firmly by a bolt (7). The initiation photosensor (9) detects the initial position of the rotor.
The heating canal (4) is insulated (6). The canal is thermostatted at 37 ºC by 4 peltiers (10), with their respective
radiators (11) and fans (12).
The rotor shaft is connected to a pulley (14) which transmits the motor movement (15) through a belt (17) and
a pinion (16). The encoder disk (13) is attached to the pulley. The photosensors (18) relays the movement of the
encoder to the electronic board.
The washing operation has 10 cycles. In each cycle two tips are used. The longest is used for suctioning and the
shortest for dispensing.
• Cycle 1(26): The reaction mixture is suctioned and the washing solution is dispensed. The suctioned waste is
sent directly to the high contamination waste bottle.
• Cycle 2 (27): The washing solution is suctioned and then dispensed again.
• Cycle 3: Wait cycle during which the wells are washed
• Cycle 4 to 6 (28): Rinsing, suctioning of liquid and dispensing of distilled water
• Cycle 7: Wait cycle
• Cycle 8: Optical checking of well
• Cycle 9 (29): Suctioning of distilled water
• Cycle 10 (30): Drying of well.
34
During the last two cycles, in the event of the tips colliding with the rotor, a sensor (21) detects this and stops the
operation.
The whole tip assembly is secured mechanically with a bolt (22).
The wash station has a built-in system for blocking the entry of light through the cover vents, part (25). This part is
always in a lower position, touching the cover. When the wash station is raised and lowered, part (25) is maintained
in its position, making contact with the cover, by means of guide elements (23) and springs (24).
The wash station tip assembly is joined to a shaft (31). This shaft is dragged by a belt (37) through part (34). In
turn, the motor (38) transmits the movement to the belt (37) through the pulley (39).
The transmission pulley is protected by part (40).
The tab (35) and photosensors (36) are used to detect the wash station start-up position.
20
21
22
23
24
25
26
27
28
29
30
Figure 3.23 Wash station tip assembly
31
32
33
34
35
36
37
38
39
40
Figure 3.24 Wash station elevation system
35
Service manual
References of Figure 3.23, and Figure 3.24:

30 – Drying
20 – Wash station housing 31 – Wash station elevation shaft
21 – Tip collision sensor 32 – Wash station shaft anchoring support
22 – Set screw 33 – Counter pulley
23 – Pedal guide shaft 34 – Part anchoring shaft with belt
24 – Spring 35 – Start-up detection tab
25 – Pedal 36 – Start-up detection photosensor
26 – Waste aspiration 37 – Belt
27 – Washing solution aspiration 38 – Wash station motor
28 – Rinsing with water 39 – Transmission pulley
29 – Water aspiration 40 – Transmission pulley protector
41
42
43
44
45
Figure 3.25 Optical bench view
46
47
48
49
50
51
52
53
54
55
56
Figure 3.26 Optical bench view
36
References of Figure 3.25 and Figure 3.26
49 – Beam splitters for each wavelength
41 – Optical bench 50 – Lenses and lens holder
42 – CIIM00051 board 51 – Rotor
43 – Cover sensor 52 – Optical bench support
44 – Temperature sensor 53 – Filters for each wavelength
45 – CIIM000050 board 54 – Filter holder
46 – Optical bench opening screws 55 – Leds for each wavelength
47 – Optical bench cover 56 – Principal and reference photosensors
48 – Optical bench seal
The optical bench is secured directly to board CIIM00051 (42). The different leds (55) for each wavelength and the
main and reference photodiodes (56) are welded to board CIIM00051 (42). The filters (53) for each wavelength are
inserted in the filter holders (54) and screwed to the optical bench support (52). To ensure that the light beam for
each wavelength hits the rotor (51) there are beam splitters (49) and lenses (50). The whole assembly is sealed by a
rubber joint (48) and a cover (47).
3.5. Pipetting arms

The pipetting arms are used to suction and dispense fluids. There are three arms, two for handling reagents and one
for handling samples.
The pipetting arms are comprised of a housing (1) that covers the tip connections (6) and the electronic board (2).
The tip and board assembly, attached to a support, is the mobile part of the arm. The assembly is raised by a guide
tube (9) and the wires and tubes connected to the tip pass through this tube. The raising operation is executed with
a motor (10) that transmits the movement through a pulley (11) to a belt joined to the guide tube (9). The angular
movement is executed with the motor (4) by means of a belt (3) which makes the guide tube rotate (9).
5
6
7
8
10
11
Figure 3.27 Pipetting arm assembly
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Service manual
12
13
14
15
16
17
Figure 3.28 Reagent tip
References of Figure 3.27 and Figure 3.28:

10 – Elevation movement motor
1 – Housing 11 – Elevation movement transmission pulley
2 – CIIM00049 board 12 – Tip thermostatting system cable
3 – Angular movement transmission belt 13 – Tip temperature sensors cable
4 – Angular movement motor 14 – Tip detection system cable
5 – Elevation movement initiation photosensor 15 – Collision detection photodiode
6 – Aspiration and dispensation tip 16 – Tip collision detection system
7 – CIIM00048 board 17 – Angular movement initiation photodiode
8 – Elevation movement transmission belt
9 – Elevation movement guide tube
The only difference between the reagent and sample arms is the tip (6).
Characteristics of the reagent tip and the sample tip:
Reagent tip Sample tip

Inner diameter at the end 0.8 mm 0.4 mm
Maximum volume contained
500 mL 40 mL
inside
Thermostatting Yes No
Temperature sensor Yes No
Fluid detection Yes Yes
The tip control board CIIM00049 (2) is the same for the reagent arm and the sample arm.
3.6. Stirring arm

The elevation and rotation movement assembly part is the same as the pipetting arm assembly.
))
See section 3.5 for a detail of the assembly.
The stirrer is formed by a flat blade(5). The blade is inserted into the continuous current motor shaft (1) and pressed
by a clamping nut (4). The motor (1) is attached to the head piece support (3) by an adapter (2).
38
1
Figure 3.29 Stirrer arm head
References of Figure 3.29:

1 – Continuous current motor
2 – Adapter
3 – Head support
4 – Clamping nut
5 – Stirrer blade
3.7. Dispensation pump assembly
3.7.1. Dispensation pump assembly

The dispensing pump assembly is comprised of three pumps. Each pump is independent and they are connected
to the reagent and sample arm tips.
The pistons of the two pumps for pipetting the reagent have a diameter of 8 mm, while the piston of the sample
pump has a diameter of 3 mm.
The fluidic system of the three pumps with the input electro valves is comprised of a PMMA manifold (1).
In the fluidic chamber of the sample pump the manifold has a pressure sensors that detects whether the sample tip
is blocked (3).
All three pumps are identical, except for the sample pump. Its piston has a smaller diameter. They are operated by
a motor (13) attached to a multi-twisting spindle (12). The spindle raises or lowers the piston support (11) where
the piston is attached. The suctioning chamber is sealed by a stop (10). The tubes are connected to the manifold
by connection fittings (7).
39
Service manual
Figure 3.30 Dispensation pump assembly
9
10
11
12
13
Figure 3.31 Detail of dispensation pumps

8 – 3 mm sample piston
1 – Manifold with electro valves 9 – 8 mm reagent piston
2 – Board with led lamp 10 – Stop
3 – Pressure sensor 11 – Piston support
4 – Sample pump 12 – Pump spindle
5 – Reagent 1 pump 13 – Pump motor
6 – Reagent 2 pump
7 – Output connection fitting
3.7.2. New dispensation pump assembly design

In the new pump assembly, each pump and its manifold have been separated and in the sample pump, the premani-
fold has been integrated with its electro valves.
40
1
2
3
Figure 3.32 New dispensation pump assembly
References of Figure 3.32: 5 – JE1-EV5 premanifold electro valve

1 – R2 reagent pump 6 – R2 internal wash pump
2 – R1 reagent pump 7 – R1 internal wash pump
3 – Sample pump 8 – Internal sample wash pump
4 – JE1-EV4 premanifold electro valve
10 18
19
11
12 20
13
14
15
16
17
Figure 3.33 Ceramic sample and reagent pump
41
Service manual

19 – LED lamp electronic board
9 – Tube connection washing solution inlet 20 – JE1-EV4 switching electro valve
10 – Tube connection sample pump chamber inlet 21 – Manifold cover
11 – LED lamp electronic board 22 – LED lamp electronic board
12 – JE1-EV4 premanifold electro valve 23 – Ceramic piston support
13 – JE1-EV5 premanifold electro valve 24 – Stop
14 – JE1-EV1 switching electro valve 25 – Stop ring
15 – Tube connection distilled water inlet 26 – Starting sensor
16 – Tube connection premanifold outlet
17 – Clot sensor
18 – Input connection of the reagent pump chamber
21
22
23
24
25
26
Figure 3.34 Dismantling of sample pump
The tube connections are as shown in Figure 3.35 and Figure 3.36.
The premanifold emerges from the tube (28) and is connected to a T-shaped interconnection fitting.
3 tubes emerge from the T, each of which is connected to one of the R2 (6), R1 (7) and sample (8) internal wash
pumps.
Tubes (29), (30) and (31) connect the internal wash pumps to the ceramic pumps.
The premanifold has 2 inlets, one at the distilled water input (27), which comes from the degasser outlet. There is
a tube diameter adapter (32).
The other inlet is for the washing solution and is connected to the intake (34), and there is also a tube adapter (33).
42
27
28
29
30
31
32
Figure 3.35 Tube connection
33
34
35
Figure 3.36 Tube connection

43
Service manual
3.8. Structure
Each of the analyser's subassemblies is attached to the structure (1). The sample rotor, reagent rotor, reaction rotor,
sample arm, reagent arm and stirring arm subassemblies are attached at the top. The pump and fluidic subassemblies
are attached at the bottom right-hand side. The electronics and power supplies are located on the top left-hand side.
The ISE module (2) is located in the centre, at the top, and the ISE module reagent kit (6) is located in the bottom
central part. See Figure 3.18.
The washing solution bottles (5) and high contamination waste bottles (4) are located in the bottom central part.
The volume in the bottles is determining by weighing. Below each bottle is a weighing scale (7).
The general fans (12), (13) are fastened directly to the structure. The fan (3) is attached to the front panel.
The wash stations for each tip and stirrer (10) are attached directly to the structure. The evacuation tubes are con-
nected directly to the low contamination waste bottle. The distilled water bottles (14) and low contamination waste
bottles (15) can be accessed from the rear bottom part. Double buoy sensors are used to detect the maximum and
minimum levels of each container.
The main switch is located at the bottom right-hand side (16), and the partial switches at the side (11).
The external fluidic connections are located at the bottom left-hand side (17).
4
5
6
8
9
Figure 3.37 Front view of the structure
44
10
11
12
13
14
15
16
17
Figure 3.38 Rear view of the structure

10 – Wash station for tips
1 – Structure 11 – Lateral switches
2 – ISE module (optional) 12 – General fans
3 – Fridge fans 13 – Electronic compartment fans
4 – High contamination waste bottle 14 – Distilled water bottle
5 – Washing solution bottle 15 – Low contamination waste bottle
6 – ISE module calibration standards kit 16 – Main switch
7 – Bottle weighing scales 17 – External fluid connections
8 – Wheel
9 – Anchoring leg
3.9. Fluid connections

The entire fluidic system is mounted on a board located on the right-hand side of the analyser.
To facilitate the identification and monitoring of the fluidic system, the tube colour selection uses the following
criterion:
• Blue: Distilled water tubes
• Red: Waste tubes
• Green: Washing solution tubes
45
Service manual
4
5
6
7
8
9
10
11
12
13
14
15
24 23 22 21 20 19 18 17 16
Figure 3.39 Fluidic system assembly

13 – SF1-EV2 electro valve
1 – SF1 board - fluidic system control
14 – SF1-B4 pump
2 – JE1 board - syringe control
15 – SF1-B5 pump
3 – Wash station tube heater
16 – Manifold
4 – SF1-B10 pump
17 – SF1-GE1 electro valve
5 – SF1-B3 pump
18 – Wash station pump
6 – SF1-B2 pump
19 – Wash station motor
7 – SF1-B1 pump
20 – JE1-EV4 electro valve
8 – SF1-B9 pump
21 – JE1-EV5 electro valve
9 – SF1-B8 pump
22 – JE1-B1 pump
10 – SF1-B7 pump
23 – JE1-B2 pump
11 – SF1-B6 pump
24 – JE1-B3 pump
12 – SF1-EV1 electro valve
The water can enter the analyser in two ways:

• Through a pressurised water system
• Through an external tank with a large capacity
The user programme will select the water entry method. In the first case, the programme will activate the electro
valve (12) and fill the intermediate distilled water tank.
In the second case the programme will activate the electro valve (13) and the pump (14), to suction water from the
external tank.
46
The internal distilled water tank level is controlled by a buoys system that will tell the programme when the tank
is full or empty.
There is also an internal low contamination waste tank. The tank level is also controlled by a buoy system.
When the system detects that the tank is full, the programme activates the pump (15) and evacuates the water
externally.
Pipetting system
Each of the tips, sample, reagent 1 and reagent 2, is connected directly to its respective ceramic pump in the mani-
fold assembly.
When the pumps are suctioning or dispensing the samples or reagents, electro valves JE1-EV1, JE1-EV2 and JE1-
EV3 will be closed.
During the internal tip washing process, these electro valves will be open and can dispense distilled water, washing
solution or air, depending on the selection made in the pre-manifold.
The pre-manifold selects water, washing solution or air, depending on the state of the electro valves (20) and (21).
Using the pumps (22), (23) and (24) it pumps the liquids selected by the sample, reagent 1 and reagent 2 tips,
respectively.
The outsides of the tips are also washed in the wash stations. Distilled water is used for washing the insides, and it
is pumped to the sample tip by pump (7), to the reagent 1 and stirring 2 tips by pump (6) and to the reagent 2 and
stirrer 1 tip by pump (5).
Wash station
The rotor washing system is comprised of 10 cycles.
)) See chapter 2.5 for a description of each wash station phase.
Dispensing from each tip is executed with a pump that activates 5 pistons. The pump is operated by the motor (19).
Each piston is connected to an electro valve (17) where the 2 first tips dispense washing solution and the other 3,
distilled water.
The tip of the first cycle suctions the high contamination waste through pump (4) and dispenses it directly into the
bottle provided for that purpose.
The tips of cycles 2 and 4 are suctioned by the same pump (11) and the suctioned material is evacuated into the
low contamination waste tank.
The tips of cycles 5 and 6 are suctioned by pump (10) and also emptied into the low contamination waste tank.
The tip of cycle 9 is suctioned by pump (9) and the tip of cycle 10 executes the drying operation through pump (8).
The last two pumps evacuate the suctioned material into the low contamination water tank.
3.9.1. Modification of the fluidic connections

In addition to the modification of the dispensation pumps, the premanifold assembly has also been modified.
47
Service manual
25
26
27
Figure 3.40 Fluidic assembly modification

25 – SF1-B10 pump
26 – Connection of distilled water to degasser
27 – Connection of washing solution to dispensation pumps
The fluidic assembly modifications are the following:

• Modification of the pumps model used to suction the high contamination waste SF1- B10 (25)
• The premanifold assembly is incorporated into the suctioning pump assembly.
48
3.10. Degasser
4 7
Figure 3.41 Degasser
Figure 3.42 Pump outlet connection tube

6 – Degasser output connection tube leading to the
1 – Vacuum control system premanifold
2 – Degasser membrane 7 – Degasser assembly
3 – Non-return valve 8 – T-shaped tube connection at pump outlet
4 – Pump to create the vacuum 9 – Low contamination waste tube
5 – Degasser input connection tube
The water used by the analyser pipetting system passes previously through the degassing circuit to eliminate any
bubbles there may be in the water.
The system is made up of a membrane (2) that suctions air from water. This membrane is positioned to operate in
series with the water circuit (5) and (6).
To ensure the membrane functions correctly, it is operated under vacuum, using a membrane pump (4) and a control
circuit (1) to monitor the vacuum pressure.
The membrane pump outlet (4) passes through a non-return valve (3) and is connected through a T-shaped inter-
connection fitting to the low contamination waste outlet.
49
Service manual
4. Electronic elements
The electronics controlling the analyser are laid out in 14 independent boards. Each board controls an analyser sub-
assembly. The method for transmitting the information between the boards is through a CAN communication bus.
There are 2 more boards which are used to make the CAN bus interconnection.
Some boards perform the same function and are repeated, for instance the boards that control the arms. To enable the
information to be communicated through the bus and reach the correct board, the board must have a single identifier
inside the analyser. For this reason each board has a series of switches so that the board identifier can be selected.
The code assigned to each board is indicated below:
Type of board Description of board Identification code Selector position

Arm boards Sample 1 arm 0000
BM1
Reagent 1 arm 1000

BR1
Reagent 2 arm 0100

BR2
Stirrer 1 arm 0010

AG1
Stirrer 2 arm 1010

AG2
Detection boards Sample 1 detection 0110

DM1
Reagent 1 detection 1110

DR1
Reagent 2 detection 0001

DR2
Rotor boards Sample 1 rotor 0101

RM1
Reagent 1 rotor 0011

RR1
50
Type of board Description of board Identification code Selector position
Fluidic system Fluidic system 1111
board SF1
Syringe board Syringes 0100

JE1
Each of the above boards has a microprocessor. To rapidly identify the microprocessor status, they have a built-in
state indicator led. The information provided by the flashing of the led is the following:
Status Description
Rapid flashing (burst) Board start-up. After a few seconds, the flashing changes to slow.
Rapid flashing Board in monitor mode.
Slow flashing Board has firmware and is operating correctly.
Very rapid flashing. With Firmware update process.
no regular sequence
Not flashing Board not supplied with power or damaged.
The analyser chassis, and all its metallic parts are only connected to the socket ground wire. This is
the electrical safety wire.
The negative reference or GND of the electrical circuit is isolated from the chassis. To measure the
NOTE voltages with a multimeter or an oscilloscope, place the instrument reference at the “test points”
marked GND in each board or the CAN connector reference connection.
4.1. Sources and input-AC board - CIIM00056
Figure 4.1 Silkscreen printing of input-AC board
51
Service manual
5 4 3 2 1
FN 284-10-06
N N
E
E
UNIVERSAL INPUT PLMA00258
94V - 264V
600W EMC
P P
1
CIIM00056
CN1
AC INPUT
FRIDGE PS MAIN PS ISE

C CN2 CN3 CN4
1
FRIDGE PS MAIN PS ISE
FA FA FA
FRIDGE MAIN ISE
SP-320-24 SP-320-24 RS-35-24
24V 13A 24V 13A 24V 1.5A
FA FRIDGE FA SF1 FA ISE

FA MAIN
FA MAIN
Figure 4.2 Power supply input connection diagram.
4.2. Distribution board - CIIM00047

5 4 3 2 1
The distribution board is responsible for making the connection for each of the different boards. The connections
are made by the CAN bus. The bus cable distributes the power for each board and the CAN signals.
Both distribution boards are identical and each connector bears silk printing indicating the subassemblies to which
they are connected.
Each connector has two silk prints, one of which is used for board A and the other for board B. Distribution board
A is the board located in the top position, and distribution board B is located in the bottom position.
52
Figure 4.3 Distribution board silk printing
Connector Function
CN1 A Connection to the CPU board
CN1 B
CN2 A Connection to the JE1 board
CN2 B Connection to the GLF board
CN3 A Connection to the SF1 board
CN3 B Connection to the AG1 board
CN4 A Connection to the DM1 board
CN4 B Connection to the AG2 board
CN5 A Connection to the DR2 board
CN5 B Connection to the BR1 board
CN6 A Connection to the DR1 board
CN6 B Connection to the BM1 board
CN7 A Connection to the RR1 board
CN7 B Connection to the BR2 board
CN8 A Connection to the RM1 board
CN8 B
CN9 Interconnection of distribution
boards, only for the CAN
CN20 Connection to the power source
LEDs Function (on condition)

LD1 Voltage of 24 V
The CAN connectors are supplied with an anchorage system to ensure their connection.
53
Service manual
If it should be necessary to remove a CAN cable, take the following steps (see Figure 4.4):
Figure 4.4 Disconnecting a CAN connector
1. Press the sides of the connector firmly (1). There are tabs on each side for insertion into the female connector.
2. Pull the connector and cable outward (2).
3. Do not pull the cable directly.
4. If you cannot remove the cable, use a pair of flat tip pliers that are wide enough to push the tabs.
All the CAN cables have three labels placed at their ends.
• One label indicates to which board it is connected and the board identifier, and is used to distinguish the cables
by their lengths, given that in terms of their construction, all the cables are identical.
• The other two labels are at both ends of the cable, one is yellow and the other is white. The end with the yellow
marks is the one that must be connected to the distribution board.
Figure 4.5 Itemised description of the CAN connector
Figure 4.5 shows a CAN connector, with the pins (1), connector (2) and extra anchoring elements (3).
54
Steps for mounting a CAN connector:
1. Place each of the pins (1) so that they compress the cable in the connector compartment (2). The pins have a
single position.
2. Insert the pins as far as they will go. The pins must be anchored.
3. Insert through the side of the connector (2) the extra anchoring (3) until it clicks into place.
4.3. Main board (CPU) - CIIM00046

This is the main board in which the tasks are distributed (in terms of the firmware) to each of the subassembly boards.
This board also controls the following devices:
• The computer communications through RS-232 and USB.
• The analyser status lamp.
• The analyser main cover sensor.
• The general fans of the analyser. These fans have two wires.
• Control in sending the commands for controlling the ISE module and receiving the results thereof.
The electronic control system for the RS-232 for communications and for the ISE module is electrically isolated
from the main electronic system. To supply it with power, use a special insulated regulator (U20 and U3).
USB supplied by the computer and insulated from the equipment electronic system.
Figure 4.6 CPU board silk printing
55
Service manual
CIIM00046
3
CN2 PLMA00286
STATUS LED
ORANGE
GREEN
1
CN1
2 CAN BUS LED STATUS BICOLOUR
3 3
2
4 2
5 LED STATUS 1
CPU CON3
1
6
CAN BUS 2
CN8 7
3 RS-232
RS-232 8
4
9
5
1
6
2
CN7 7
3
ISE 8 RS-232 -ISE MODULE
4
9
5
5
4
CN10 3
2 USB
USB 1
CN21 FAN
1
F.FAN1 2 CPU F. FAN1
CN20
1
2
CN21
1
2
PLMA00285 1 6
CN3 5
1 2 4
DETECTOR COVER
COVER SENSOR 2 COVER SENSOR
Figure 4.7 CPU board connections
Connector Function Pin

CN2 CAN bus connection Pin 1: 24V
Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN1 Led status lamp (3-coloured) Pin 1: Activation of LED1
Pin 2: GND
Pin 3: Activation of LED2
CN7 ISE module serial channel Pin 2: Transmission
Pin 3: Reception
Pin 5: GND
CN8 Analyser serial channel Pin 2: Transmission
Pin 3: Reception
Pin 5: GND
CN10 USB connection Pin 1: 5 V
Pin 2: USB+
Pin 3: USB-
Pin 4: GND
56
CN19 General fan Pin 1: 24 V
Pin 2: Activation
Pin 2: Activation
Pin 2: Activation
Test point Function

TP1 5V
TP7 GND ISE
TP18 ISE transmission serial channel
TP19 ISE reception serial channel
TP26 GND serial channel
TP27 Transmission serial channel
TP28 Reception serial channel
TP29 USB + signal
TP31 USB - signal
TP33 USB transmission serial channel
TP34 USB reception serial channel
TP38 3.3 V
TP40 24 V
TP41 GND
TP42 CAN_H (bus signal)
TP43 CAN_H
TP44 CAN_L (bus signal)
TP45 CAN_L
TP48 5V
TP49 3.3 V
TP50 GND
TP51 GND
TP52 5V
TP76 5V USB
TP78 5V USB
TP87 Fan activation voltage
TP90 Buzzer activation voltage

LD3 3.3 V voltage
LD5 Insulated 3.3 V voltage
LD6 CPU start-up indication
LD7 CAN start-up indication
57
Service manual
4.4. Arm boards - CIIM00048

These boards are located on each of the five arms.
The board has a micro controller (U1) which controls the drivers of the motors that move the arm vertically (U7)
and horizontally (U8).
It also contains the electronic control system of the start-up sensors for each motor.
CIIM00048 1 6
PLMA00324 5 HORIZONTAL
CN14
2 4 DETECTOR
CN16 3
MOTOR H
MOTOR H 2
1 1
2
3 CAN BUS
4 CON3 1
5 CN10 2
PLMA00323 3
1 HORIZONTAL
2
HOME H 3 HOME H MOTOR
6
4
BR2
BR1
BM1
CN15
AG1 3
AG2 HOME V 2
1
1 6
CON3 PLMA00325 5 VERTICAL
CN10
2 4 DETECTOR
1 HOME V
2
MOTOR V 3
4
1
2
PLMA00326 3
VERTICAL
MOTOR V MOTOR
4
Figure 4.8 Arm board connections
Figure 4.9 Arm board silk printing.
58
Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN7 Vertical motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN10 Horizontal motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN14 Horizontal start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN15 Vertical start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
Test point Function

TP1 Horizontal motor reference voltage
TP2 Horizontal stepper motor
TP3 Vertical motor reference voltage
TP4 Board selection. Direction 1
TP5 5V
TP6 24 V
TP8 3.3 V
TP9 Vertical stepper motor
TP12 24 V (bus voltage)
TP13 GND (bus voltage)

LD2 Board start-up status
LD6 3.3 V voltage
LD7 Vertical arm start-up detection
LD8 Horizontal arm start-up detection
59
Service manual
4.5. Tip detection board - CIIM00049

Board located in the upper part of the sample and reagent arms. These boards control the following elements,
through a micro controller (U10):
• Fluid detection system
• Tip collision sensor
• The heating element and temperature sensor only in the reagent tips.
• Protection against ESD in the tip.
CIIM00049
1
PLMA00330 +tº
PROBE
TEMPERATURE
CN2
TP SENSOR
2
CN1 1
1
TP 2
2 CON2
3 CAN BUS
4
5
1
CN3
PLMA00329
1 PROBE
DR1 HP 2 RESISTANCE
HP
DM1
2
CN4
3
DN 2
1
CON3 PLMA00328
PROBE
DN DETECTOR
Figure 4.10 Tip board connections
60
Figure 4.11 Tip board silk printing

Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN2 Tip temperature sensor Pin 1: 3.3 V
Pin 2: Sensor
CN3 Tip resistance Pin 1: Resistance
Pin 2: Resistance
CN4 Detection of tip Pin 1: Detection signal
Pin 2: Injection signal
Test point Function

TP1 5V
TP6 3.3 V
TP19 Tip collision detection
61
Service manual

LD1 CPU status
LD2 Collision detection
LD3 Sample detection status
4.5.1. Level detection adjustment

Material required to make the adjustment:
• Plastic, flat-tip precision screwdriver. Bourns or similar trimming tool.
A plastic screwdriver must be used, to prevent perturbations in the detection signal while the power meter turns.
Do not use a metal screwdriver as it will affect the signal.
The state of led LD3 indicates the detection frequency adjustment. The process must be made when the analyser is
on stand-by and the tip is correctly primed.
If the led flashes or if off, this indicates that the detection frequency is not adjusted.
As the adjustment frequency is approached, the led will flash more rapidly until it remains on when the target
detection frequency is reached.
The detection frequency for the sample tip is: 2M Hz
The detection frequency for the reagent tip is: 1M Hz
Steps for making the adjustment:
1. Turn on the analyser
2. Remove the cap from the tip you want to adjust
3. Insert the plastic screwdriver into the power meter (P1). Ensure that the tip is correctly primed and not touch-
ing any fluid.
4. Gently turn the power meter in the direction in which the LD3 led flashing frequency increases until it stops
flashing.
5. If the frequency falls, change the direction for turning the power meter.
Potential problems in the tip detection adjustment:
Detection problem Solution

It is impossible to achieve the stable Check that the cable protective mesh is correctly compress on the
flashing of the LD3 adjustment led connector pin. Check that the cable is not pinched.
Check that the tip detection cable is properly attached to the board
with a clamp.
Check that the screws, nuts and parts securing the collision detection
spring are properly tightened and do not move.
Check that the tip is correctly primed.
62
4.6. Photometric control board - CIIM00050
Board located in the reading rotor. It contains a micro controller (U5) which controls the following elements:
• Reaction rotor cover detection (U7)
• Wash station motor (U14)
• Wash station start-up detection (U10)
• Wash station collision detection (U10)
• Photometric board connection
• Reaction rotor motor (U12)
• Reaction rotor start-up detection (U10)
• Reaction rotor encoder detection (13)
• Peltier control in thermostatting the reaction rotor (U18)
• Fan control for cooling the peltiers (U17). They are fans with 3 wires.
• Reaction rotor thermostatting temperature sensor control (U15 and U16)
CIIM00050 1 6
CN1 5
ROTOR
1
PLMA00321 DETECTOR
2 CAN BUS 2 4
3 CN8
4 3
HOME ROTOR
GLF1 5 2
HOME ROTOR 1
CAN BUS
CON3 1
CIIM00051 2
CN6 3
PLMA00316 1
PLMA00307 ROTOR
CN5 ROTOR 2 MOTOR
CN1 3
PHOTOMETRY PHOTOMETRY ROTOR
6
4
CN7
CN3 ENCODER 1
ROTOR 1 2
PLMA00320 2 1 6 1 6 1 6
COVER 1 3 5 5 5
ENCODER
2 ROTOR COVER 4 ROTOR
DETECTOR ROTOR COVER 5 2 4 2 4 2 4
PLMA00319
ENCODER
PLMA00315 CN17 CN4
PELTIER 3
1 2 1 6
WASHING
2 1 5 STATION
WS COLISION PLMA00318
2 4 COLLITION
PLMA00313 CON3 WS COLLISION DETECTOR
CN15
FAN
1
2 FAN CN10
HOME 3
2 1 6 WASHING
WASHING ST. 1 PLMA00308 5
PLMA00312 STATION
CN14 2 4
FAN CON3
HOME
1 HOME WASHING ST.
2 FAN
CN9
1
PLMA00314 WASHING 2
CN16 3
PELTIER STATION 4 1
1 2
2 3
WASHING
PLMA00306 STATION
MOTOR
PLMA00311 WASHING STATION
4
CN13
FAN
1
FAN 2 FAN
1
PLMA00309 CN12 CN11 PLMA00310

FAN +tº TEMPERATURE
1 THERMISTOR 1 SENSOR
FAN 2 FAN 2 THERMISTOR
2
Figure 4.12 Photometric control board connections
63
Service manual
Figure 4.13 Photometric control board silk printing

Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN3 Rotor cover detection Pin 1: 5V
Pin 2: Cover detection
CN4 Wash station collision detection Pin 1: GND
Pin 2: Wash station detection
Pin 3: GND
Pin 4: Wash station collision
CN5 Photometric board connection
CN6 Rotor motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN7 Rotor encoder Pin 1: GND
Pin 2: C channel encoder
Pin 3: A channel encoder
Pin 4: 5 V
Pin 5: B channel encoder
64
CN8 Rotor start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN9 Wash station motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN10 Wash station detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN11 Tip temperature sensor Pin 1: 3.3 V
Pin 2: Sensor
CN12 Fan Pin 1: 24 V
Pin 2: Detection diagnostic
Pin 3: Activation
Pin 3: Activation
Pin 3: Activation
Pin 3: Activation
CN16 Peltier Pin 2: Peltier activation
Pin 3: Peltier activation
CN17 Peltier Pin 2: Peltier activation
Pin 3: Peltier activation
Test point Function

TP1 24 V
TP2 GND
TP5 GND
TP6 GND
TP14 12 V insulated
TP16 3.3 V
TP17 5V
TP18 24 V
TP19 GND
TP27 Rotor motor reference voltage
TP28 Motor driver step voltage
TP40 Wash station motor reference voltage
65
Service manual
Test point Function

TP41 Motor driver step voltage
TP51 Rotor temperature

LD1 3.3 V activation
LD2 CPU start-up
LD3 Wash station rest phase detection
LD4 Rotor start-up detection
LD5 Wash station start-up detection
4.7. Photometric readings board - CIIM00051

This board is located below the optical bench in the reaction rotor assembly. The board has the leds for each wave-
length and the two photosensors directly welded to it.
This board controls the switching on and off of each led. The leds are controlled through a power source (U2, U8,
U9 and T13) and the led on selection is done through a decoder (U11).
The readings are made through two photodiodes (main and reference photodiodes) which are read by a double
integrating ramp converter (U1, U4, U5 and U6).
All the control signals are sent to the photometric control board (CIIM00050) through an I2C channel (U7).
Figure 4.14 Photometric control board silk printing

CN1 Photometric board connection
Test point Function

TP3 LED voltage
TP4 LED voltage
TP6 Decoder 1
66
Test point Function
TP7 Decoder 2
TP8 Decoder 3
TP9 Decoder 4
TP10 Activation of LED 1

LD1 340 nm LED
LD2 560 nm LED
LD3 670 nm LED
LD4 600 nm LED
LD5 535 nm LED
LD6 505 nm LED
LD7 635 nm LED
LD8 405 nm LED
LD9 Free position
LD10 Free position
LD11 Free position
LD12 I2C decoder indicator
4.8. Rotor boards - CIIM00052

The analyser has two CIIM00052 boards which are located in the sample and reagent rotor assemblies. Both boards
have the same PCB but the fridge components have not been mounted on the sample rotor board. Each board has
a different board selection switch code.
Elements that are common to both boards

• Micro controller (U5)
• Cover sensor (U3)
• Control of fans located in the structure (U3 and U6)
67
Service manual
• Rotor motor (U8)

• Rotor start-up detection (U3)
• Barcode reading communication (U3 and U4)
Fridge components mounted on the reagent rotor board

• Separate power supply (U16)
• Micro controller (U13)
• Reading window demisting driver (U10 and U17)
• Thermistor for controlling fridge
• Fridge peltier control (U9 and U11)
• Fridge peltier fans (U9 and U11), controlled by the same drivers as the peltiers
CIIM00052-A : REAGENT CIIM00052 B-PLMA00332 1 6

CN26 5
CIIM00052-B : SAMPLE 1
A-PLMA00336 ROTOR
2 CAN BUS 2 4 DETECTOR
3 CN11
4 3
ROTOR HOME
5
ROTOR 2
CAN BUS HOME 1
CN2 1
FA FRIDGE COOLER B-PLMA00333 2
FRIDGE SUPPLY 1 POWER CN9 3
2 1
A-PLMA00337
SUPPLY 2
ROTOR
ROTOR 3 ROTOR MOTOR
6
4
B-PLMA00334
CN8
COVER 1 2
A-PLMA00335 1
DETECTOR 1 6
2 ROTOR COVER 2
ROTOR COVER 7
3
BARCODE
CN4 8 READER
BARCODE 4
BRANCH2 9
PELTIER CN13 READER 5
1 PELTIERS
2 BRANCH2 RS-232 -BAR CODE READING
FRIDGE FAN4 CN14

FAN 1
2 P.FAN4
3
1
PLMA00338
CN3
HEATER
FRIDGE FAN3 CN15 DEFOGGER 1 DEFOGGER
2
FAN 1 2
2 P.FAN3
3
CN6
BRANCH1 CN12
PELTIER 1
1 PELTIERS 2
2 BRANCH2
FAN2 CN16 CN7

FRIDGE 1
FAN 2
P.FAN2 1
3 2
1
FRIDGE FAN1 CN19 PLMA00345

FAN 1
+tº TEMPERATURE
2
P.FAN1 CN16 SENSOR
3
THERMISTOR
2
1
THERMISTOR 2
Figure 4.15 Rotor board connections
68
Figure 4.16 Rotor board silk printing

Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN2 Separate fridge power supply Pin 1: 24 V Fridge
Pin 2: GND Fridge
CN3 Demister Pin 1: 24 V
Pin 2: Activation
CN4 Barcode reader Pin 1: Activation/deactivation
Pin 2: Reading channel 1
Pin 3: Transmission 1
Pin 4: GND
Pin 6: Reading channel 2
Pin 9: Reading shot
Pin 10: Transmission 2
Pin 2: GND
69
Service manual

Pin 2: GND
CN8 Cover sensor Pin 1: 5V
Pin 2: Detection signal
CN9 Rotor motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN11 Rotor start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN12 Peltier Pin 1: 24 V
Pin 2: Peltier diagnostic
Pin 4: GND
CN13 Peltier Pin 1: 24 V
Pin 4: GND
CN14 Fridge fan Pin 1: 24 V
Pin 3: GND
Pin 3: GND
CN16 Temperature sensor Pin 1: Sensor
Pin 2: GND
Pin 3: GND
Pin 3: GND
Test point Function

TP1 24 V
TP2 5V
TP5 CAN_H
70
Test point Function
TP11 24 V Fridge
TP12 5 V Fridge
TP13 CAN_L
TP19 Rotor stepper motor

LD1 3.3 V voltage
LD2 3.3 V voltage Fridge
LD3 Barcode reader activation
LD4 Microprocessor start-up
LD5 Cover detection
LD7 Rotor start-up detection
LD9 Fridge peltier activation
LD10 Fridge peltier activation
LD12 Fridge microprocessor start-up
4.9. Fluid control board – CIIM00053

This board is located at the top of the fluid zone.
The board controls the following elements, through a micro controller (U10):
• Stirrer driver (U18)
• Scales signal conditioning (U5 and U6) for the washing solution and high contamination waste
• Internal water and waste bottle buoy conditioning
• Driver for the wash station water heater (U1)
• Temperature sensor for controlling the thermostatting of the wash station water
• Wash station water dispensing driver
• Wash station water dispensing start-up detection
71
Service manual
SF1 1
CIIM00053 1 6
CAN BUS 2 CN3 5
WASHING
3
WPUMP STATION
4
CAN BUS 2 4
5 CN26 DETECTOR
HOME 3
ALTERNATIVE FA SF1 2
1
WPUMP 1
SUPPLY 2
CN4
SF1-B1 1
PUMP 1 2 CN19 2
1 3
2 B1 1
SF1-WPUMP WASHING
2 STATION
SF1-B2 CN20 CN26 3
MOTOR
6
1 2 1 4
PUMP 2 B2
CN21
1 1
1 2
SF1-B3 2 B3 2
SENSOR WATER
PUMP CN6 3
WATER SENSOR
CN9 4
1
2 B4
1 2
SF1-B4 1
SENSOR WASTE
PUMP CN12 2
WASTE SENSOR
1 CN5 3
2 B5 4
1 2
SF1-B5
PUMP 1
CN7 2
HCW
3
HIGH CONCENTRATION WASTE
HCW 4
1 2
SF1-B6
PUMP CN11
1 1
2 B6 CN8 2
WS
3
WS
1 2
SF1-B7 CN10
WS 4
PUMP 1
2 B7
+tº
SF1-B8 CN16 THERMISTOR TEMPERATURE
1 2 1 1 2
PUMP 2 B8 CN18 SENSOR
1
CN15
THERMISTOR 2
1
1 2
SF1-B9 2 B9
PUMP
CN14 CN17 SF1-HEATER 1 2
1 1 HEATER
2 B10 HEATER 2
1 2
SF1-B10
PUMP
1 CN28 PLMA00256 1 2
2 1 THERMAL FUSE
3 THERMAL 2 THERMAL PROT.
4 PROT.
5 CN13
5xEV SF1-GE1 6 CN2 PLMA00302 2 1
7 GE1 1 DC MOTOR
8 ST2 2 ST2
9
10
EV SF1-E1 CN1 PLMA00303 2 1
CN22 1 DC MOTOR
1 ST1 2 ST1
2 E1
EV SF1-E2 CN23 CN24
SF1-EV3
1 1 EV
2 E2 E3 2
Figure 4.17 Fluid control board connections

CN1 Activation stirrer 1 Pin 1: Output 1
Pin 2: Output 2
CN2 Activation stirrer 2 Pin 1: Output 1
Pin 2: Output 2
Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN4 Extra power Pin 1: 24 V
Pin 2: GND
CN5 Waste bottle level detection sensor Pin 1: GND
Pin 2: Bottom sensor
Pin 3: GND
Pin 4: Top sensor
72
CN6 Distilled water bottle level detection sensor Pin 1: GND
Pin 2: Bottom sensor
Pin 3: GND
Pin 4: Top sensor
CN7 High contamination waste bottle scales signal Pin 1: GND
Pin 2: Signal 1
Pin 3: Signal 2
Pin 4: 3.3 V
CN8 Wash station scales signal Pin 1: GND
Pin 2: Signal 1
Pin 3: Signal 2
Pin 4: 3.3 V
CN9 B4 pump Pin 1: 24 V
Pin 2: Activation signal
CN13 GE1 wash station dispensation electro valve Pin 1: 24 V
assembly Pin 2: Activation signal
Pin 3: 24 V
Pin 5: 24 V
Pin 7: 24 V
Pin 9: 24 V
CN17 Wash station water thermostatting Pin 1: Output 1
Pin 2: Output 2
CN18 Wash station thermistor Pin 1: 3.3 V
Pin 2: sensor signal
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Service manual

CN22 E1 electro valve Pin 1: 24 V
CN26 Wash station water dispensation motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN27 Wash station water dispensation start-up Pin 1: Detection
detection Pin 2: GND
Pin 3: 5V
CN28 Wash station water thermostatting thermostat Pin 1: output 1
Pin 2: GND
Figure 4.18 Fluid control board silk printing
Test point Function

TP4 5V
74
Test point Function
TP8 Stirrer DC voltage
TP9 3.3 V
TP11 Motor driver reference voltage
TP12 Stepper motor

LD1 3.3 V voltage
LD2 Heater activation
LD3 Activation of pump 1
LD6 Activation of electro valve 1
LD10 Motor start-up detection
4.10. Syringe control board - CIIM00054

This board is located at the top of the fluid zone.
The board controls the following elements, through a micro controller (U1):
• Sample circuit pressure sensor (clot sensor)
• Manifold led power supply
• Sample dispensation motor driver
• Sample dispensation start-up sensor
• Reagent 1 dispensation motor driver
• Reagent 1 dispensation start-up sensor
• Reagent 2 dispensation motor driver
• Reagent 2 dispensation start-up sensor
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Service manual
CN3
1
CIIM00054
JE1 2 1 6
CAN BUS 3 5
REAGENT 1
4
HOME REAG1 PUMP
5
CAN BUS 2 4
CN19 DETECTOR
3
PS4 CN2 2
CLOT 1 2
SENSOR JE1 1
HOME 1
DETECTOR 2
CLOT REAG1
3 SENSOR
1
2 REAGENT 1
CN6 CN18 3
EV1-JE1 1
REAG1 PUMP
EV 1 2
2 EV1 3 MOTOR
REAG1
6
4
CN7
EV4-JE1
EV 1
2 EV4
CN8
EV3-JE1
EV 1 1 6
2 EV3 5
REAGENT 2
HOME REAG2 PUMP
CN9 2 4
EV2-JE1 CN17 DETECTOR
EV 1 3
2 EV2 2
HOME 1
EV5-JE1 CN12 REAG2
EV 1 1
2 EV5 2
CN16 3
CN13 1
REAG2 REAGENT 2
JE1-GE3-FAN 2 PUMP
EV 1 3
GE3 REAG2 MOTOR
6
2 4
CN14
1
2 GE2
CN15 1 6
SAMPLE
EV6-JE1 5 PUMP
EV 1 HOME SAMPLE
2 EV6 2 4 DETECTOR
CN21
B2-JE1 3
1 2 CN4 2
PUMP HOME 1
1 SAMPLE
2 B2
B1-JE1 1
1 2 CN5 2
PUMP CN20 3
1 1
SAMPLE SAMPLE
2 B1 2 PUMP
SAMPLE 3
MOTOR
6
CN10 4
1
2 GE1
CN11 CN22
1 2
B3-JE1 PLMA00244 1 2
PUMP 1 LED 1 LED MANIFOLD
2 B3 MANIFOLD 2
Figure 4.19 Syringe control board connections

CN2 Pressure sensor Pin 1: 5 V
Pin 2: Sensor
Pin 3: GND
Pin 2: GND
Pin 3: NC
Pin 4: CAN_H
Pin 5: CAN_L
CN4 Activation of pump B2 Pin 1: 24 V
CN6 Activation of electro valve EV1 Pin 1: 24 V
76
CN10 Activation of pump GE1 Pin 1: 24 V
CN13 Activation of electro valve GE3 Pin 1: 24 V
CN14 Activation of electro valve GE2 Pin 1: 24 V
CN16 Reagent 2 dispensation motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN17 Reagent 2 start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN18 Reagent 1 dispensation motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN19 Reagent 1 start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN20 Sample dispensation motor Pin 1: Coil 1
Pin 2: Coil 1
Pin 3: Coil 2
Pin 4: Coil 2
CN21 Sample dispensation start-up detection Pin 1: Detection
Pin 2: GND
Pin 3: 5V
CN22 Manifold led Pin 1: 5 V
Pin 2: GND
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Service manual
Figure 4.20 Syringe control board silk printing
Test point Function

TP1 GND
TP8 24 V
TP9 5V
TP13 3.3 V
TP14 Reagent 2 motor reference voltage
TP15 Reagent 2 stepper motor driver voltage
TP16 Reagent 2 motor reference voltage
TP17 Reagent 2 stepper motor driver voltage
TP18 Sample motor reference voltage
TP19 Sample stepper motor driver voltage
78
LD2 3.3 V voltage
LD15 Reagent 2 motor start-up detection
LD16 Reagent 1 motor start-up detection
LD17 Sample motor start-up detection
4.11. Loading of firmware

The firmware loading process is executed through the service programme.
))
See chapter 8.6.8 Updating firmware.
A single file will be distributed, containing the individual firmware for each board. The process consists of first send-
ing the file to the main board (CPU) which stores it in a flash drive or repository. Once it has been verified that the
file sent is correct, the service programme will ask the user to confirm the start of the CPU board firmware updating
process. Once the CPU board has been updated, it checks the compatibility of the versions of each of the boards
against those in the repository. If any board has a version that is not compatible with the one in the repository, it
updates the firmware of that board.
While the firmware file is transmitted with the service programme, the analyser status led will flash and change colour.
All the analyser adjustments are stored in the CPU board memory. When the analyser is initialised, the necessary
adjustments for each board are sent.
As a precaution, before performing any operation with the analyser it is advisable to make a
backup copy of the adjustments.
)) See chapter 8.6.9 Saving and recovering adjustments.
NOTE
When the technical service has to carry out a repair and change a board, the technician will start the service pro-
gramme for it to accept the firmware updating process and the adjustments for the new board.
The service programme can also be used to restore the initial adjustment values.
If that process is executed, the technician will have to readjust the analyser in full.
))
See chapter 8.6.10 Restoring initial values
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Service manual
4.12. Electronic element specifications
Subassembly Electronic element Reference

Fridge Reagent rotor temperature Ermec thermistor B57861-S302-F40
sensor (fridge) 25 °C 3K Ω
Cover sensor (samples, reagents IMA.REED A041 1D 2H0500
and reaction)
Fridge peltier Thermo TEC1-12706 EPS
Fridge fan SUNON EE80252Ba-000U-G99
Barcode window heater Adhesive heating foil. 24V 6W
1EFEMI710001
Wash station Resistance MAXIWAT. Heater cartridge D8 L100 24V 75 W
heater Temperature sensor Ermec thermistor B57861-S302-F40
25 °C 3K Ω
Safety protector S105H-WTC
Reaction rotor Temperature sensor Ermec thermistor B57861-S302-F40
25 °C 3K Ω
Cover sensor (samples, reagents IMA.REED A041 1D 2H0500
and reaction)
Peltiers Thermo CP1.0-27-05L-EP
Fans Sunon EB60252S1-000U-C99
Stirrers Stirrer motor Mabuchi. FK-130RH-15210
Clot sensor Pressure sensor PAA-7LC 80933.80
0 - 5 bars
Scales Strain gauge HMB IBERICA DF2SR-3 20K
General fan Fan Sunon ME80252V1-000U-A99
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Service manual
5. Fluidic elements
The analyser operates with 4 tanks for processing fluids. These tanks are: the distilled water tank, low contamination
waste tank, washing solution tank and high contamination waste tank.
The distilled water and low contamination waste tanks are located at the bottom rear part of the analyser, and can
only be accessed by the technical service. Full and empty level control is executed through a double-buoy system.
The washing solution and high contamination waste tanks are located at the front bottom part of the analyser. They
can be accessed directly by the user.
• Once the determination has been made, the reagent and sample mixtures are sent to the high contamination
waste tank. The tank has a capacity of 5 L and autonomous operation of at least 20 h.
• The tank that contains washing solution has a capacity of 5 L and autonomous operation of at least 8 h. The
washing solution is used in the wash station to clean the reaction rotor and during the analyser initialisation
and finalisation processes.
• All the waste from the reaction rotor wash station is deposited in the low contamination waste tank, in addition
to the waste from the tip and stirrer wash stations. The tank has a capacity of 5 L and automatically empties
the content outside the appliance through a buoy control process. The system uses the SF1-B5 pump to empty
the tank.
• The internal distilled water tank has a capacity of 5 L. The water is used to prime the pipetting system, wash the
inside and outside of the sample and reagent tips and wash the reaction rotor. The tank has two water inlets that
are selected by the software. One inlet is from an external tank. For this purpose the analyser uses the SF1-EV2
and the SF1-B4 pump. The other inlet is through a water mains connection. When the water mains connection
is selected, in the event that there is pressure, only one SF1-EV1 electro valve is used.
The analyser fluidic diagram is shown below.
81
    
       
      
      
         
  

          
          
                      
            
              
      
       
Figure 5.1 Fluidic diagram

          
                    
          
    
     
        
   
 
82
       
           

       
                         
           
     

    
             
             
         
     
                       
   
         
                            
                            
                     
     
  
        

Service manual
  
  
  
  
     
                           

                      
                 
          

            
       
Figure 5.2 Enlarged view of the fluidic diagram


     
      
83
 
     
Service manual
6. Service programme
The service programme enables the analyser to be adjusted and checked, and is also used to locate failures in the
analyser.
6.1. Identification of the programme parts.

The programme screen has two parts which are common to all the menus. See Figure 6.1.
1 – Menu bar 4 – Adjustment panel

2 – Help panel 5 – Status icons
3 – Lateral bar 6 – Message lines
Figure 6.1 Screen format
Name Description
Menu bar This gives access to the programme menus.
Help panel An incrusted help screen indicates the adjustment process for each type of adjustment
on a step-by-step basis.
Lateral bar Analyser operation icons.
Adjustment panel Panel with different options for adjusting the positioning.
Status icons Icons that permanently indicate the status of certain elements in the analyser.
Message line Status line indicating messages for executing actions. The messages are shown in 2
locations.
84
6.1.1. List of common icons
The following chart shows the meanings of the common icons:
Icon Name Description

Edit Enables values to be edited.
Delete Eliminates an element.
Adjustment Initiates the adjustment process.
Save Saves the adjustment made.
Undo Undoes and recovers the last adjustment.
Close Closes the window.
Accept Accepts the changes and closes the window.
Print Launches a print request.
6.1.2. Vertical bar icons list

The vertical bar icons list shows the icons that perform actions directly on the analyser. The following chart contains
a description of each one.
Icon Name Description

Stop This icon is used to immediately stop an execution in course.
Connect This icon is used for connecting the programme with the analyser.
Shut down This icon is used to stop and shut down the analyser.
6.1.3. Explanation of the adjustment text box

The following text box is the same for the whole service programme.
It is used to position the mechanical elements of the analyser, such as the arms, rotors, etc.
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Service manual
Element Name Description

Range Permitted range Indicates the permitted range of the units displayed.
Steps Units Indicates the magnitude units. They are usually steps.
Initial position Moves the mechanical element to its initial position.
x1 Unit increments Makes the increments one by one.

x10 Increments by Makes the increments by tens.
tens
x100 Increments by Makes the increments by hundreds.
hundreds
Left steps Executes a mechanical movement to the left, one, ten or one hundred steps,
depending on the above selection.
Right steps Executes a mechanical movement to the right, one, ten or one hundred steps,
Upward steps Executes a mechanical movement upward, one, ten or one hundred steps,
Downward steps Executes a mechanical movement downward, one, ten or one hundred steps,
Accept value Executes an absolute movement. A numerical value can be entered directly in
the box and then the movement is executed by pressing this button.
6.1.4. Description of the status icons

Information about the analyser appears constantly at the bottom of the screen. See Figure 6.2.
Figure 6.2 Status icons
The following chart shows the meaning of each element:
Element Description
High concentration bottle Indicates the high concentration waste bottle level
level
Reaction rotor temperature Shows the reaction rotor temperature
Fridge temperature Shows the fridge temperature
Reagent 1 temperature Shows the temperature of the reagent 1 arm
Reagent 2 temperature Shows the temperature of the reagent 2 arm
Wash station heater Shows the wash station heater temperature
temperature
New washing solution bottle Indicates the washing solution bottle level
Main cover Indicates the status of the main cover of the analyser
Reagent cover Indicates the status of the reagent cover
86
Element Description
Sample cover Indicates the status of the sample cover
Reaction cover Indicates the status of the reaction cover
Connected Indicates the status of the analyser connection with the programme
6.2. Programme initialisation

To start up the programme, double click on the icon located on the desktop:
or execute the programme from the following route:

Start/All Programmes/Biosystems/BA400 Service
When the programme is started a welcome screen is displayed and then the screen for identifying the user, as shown
in Figure 6.3.
Figure 6.3 Home screen
Enter the username and password to access the programme.
Field Value
Username SERVICE
Password BA400
6.3. Description of the menus

The following chart shows the content of each programme menu.
Menu Name Description

Setup It gives access to the different programme setup options.
• General
• Language
• Barcode
• Users
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Service manual
Menu Name Description

Adjustments/Test Gives access to the different options for adjusting and checking the different analyser
subassemblies
• Positioning
• Photometry
• Bottle level
• Motors, valves and pumps
• Thermostatting
• Collision detection
• Barcode
• ISE module
• Stress mode
Utilities Gives access to the utilities assembly
• Conditioning
• Demo mode
• Analyser information
• Hardware versions
• Updating of firmware
Recording and Gives access to the report options
maintenance • Historical reports
• TAS report
• Preventive maintenance
Exit Exit options
• With Shutdown
• Without Shutdown
Help Help options
• Manuals
• About
6.4. Setup
6.4.1. Analyser
Enables the following options to be configured:
Selection of the water inlet: Select the water inlet mode for the analyser. The water may enter through two dif-
ferent channels which are mutually exclusive:
• Water tank
• Water mains
Deactivate buzzer: Check this option if you do not want the beeper to sound when an alarm is indicated.
Deactivate other elements: Allows you to deactivate or activate the following elements separately. These values
are stored in the analyser memory and applied when the user programme is executed.
• General cover of the analyser
• Reaction rotor cover
• Sample rotor cover
• Reagent rotor cover
88
• Clot sensor
Figure 6.4 Analyser setup screen
6.4.2. Communications
Enables the configuration of the communication channel between the service programme and the analyser. Select
one of the following options:
Automatic: Select this option and the programme will automatically search for the computer output port for
communicating with the analyser.
Manual: Select this option for the port to be selected manually.
Type of connection: In the manual mode, select the type of connection you want
• RS-232 — you should normally select the COM1 port
• USB — you should normally select the USB1 port
Press the button to ensure that the communications are functioning properly.
Figure 6.5 Communication setup screen
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Service manual
6.4.3. Language
This allows you to change the application language. There are two languages: Spanish and English.
6.4.4. Barcode
This enables the barcode readers to be activated/deactivated and the barcode options to be configured.
Figure 6.6 Barcode reader setup screen
• Deactivation of barcode for reagents: Check this option to deactivate the barcode reader of the reagent rotor.
• Deactivation of barcode for samples: Check this option to deactivate the barcode reader of the sample rotor.
• Type: Select the barcode type used for printing the labels on the sample tubes.
• Total barcode size: Select the total size of the barcode for the sample reader. The reader will only read the
codes of the indicated size, otherwise, it will mark the reading as a code error.
• External ID: If the barcode has more information than the patient identifier, select the start and end of the
patient identifier.
• Sample Type: If the code has the sample type information, select the sample type option, start and end inside
the code and how the laboratory encodes each sample type.
6.4.5. Users
Figure 6.7 shows the screen for creating and maintaining users.
Click on the icon again to obtain access to creation of new users. The boxes for entering the user data are activated.
• User ID: Enter a name for identifying the user in the application.
• Name: Enter the name of the user.
• Surname: Enter the surname of the user.
• Password: Enter a password.
• Confirm password: Enter the same password again to ensure it was entered correctly.
Press save to save the user name or undo to rule out a user or changes.
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Figure 6.7 User creation screen
6.5. Adjustments/Test
6.5.1. Adjust positioning

This menu is used to access the screens for adjusting the positions of the different mechanical elements of the ana-
lyser, such as arms, rotors, etc.
To adjust some elements, a mechanical adjustment must be made as well as an adjustment in the service programme.
6.5.1.1. Optical centring adjustment

This adjustment is used to centre each well in the reading rotor with respect to the optical system light beam. The
firing point used to make the reading is also adjusted with respect to the centring of the rotor.
The screen displays the reading profile of 5 wells. The adjustment box is used to position the vertical lines in the centre
of the wells. To do this, move the rotor step by step until the reading tip is in the centre of the wells.
If a reading is saturated or has insufficient light, move the sliding led current device to increase or decrease the
luminous intensity of the reading led.
1. Press the adjustment start button. The programme will make the optical reading of 5 wells in the rotor.
2. Step by step, move the encoder firing point with the adjustment box until the indicator line (4) is correctly
centred with respect to the well.
3. Save this position.
4. Bear in mind that if this value is changed, all the other mechanical elements will have to be adjusted.
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1 – Encoder steps 3 – Rotor optical reading

2 – Encoder reading 4 – Encoder firing adjustment
Figure 6.8 Wash station adjustment screen
6.5.1.2. Wash station adjustment

This function is used to adjust the height of the wash station. The wash station centring must be manually adjusted
beforehand.
Manual adjustment of the wash station positioning:

The manual adjustment process has two separate movements; vertical and horizontal. Each one is set using different
screws.
Figure 6.9 Wash station adjustment
1. Loosen the screw (1) anchoring the wash station in the vertical direction.
2. Move the wash station head until the tips are not touching the sides of the rotor.
3. Tighten the screw (1).
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4. Loosen the screw (2) anchoring the wash station in the horizontal direction.
5. Move the wash station head until the tips of the station are properly centred with the rotor.
6. Tighten the screws (2).
After making the mechanical adjustment, adjust the height of the wash station with the service programme.
See Figure 6.10.
Figure 6.10 Wash station position adjustment screen
1. Press the adjustment start button.

2. Move the vertical positioning of the wash station using the adjustment box.
3. Step by step, move the wash station to lower the station tips until the tip of the dryer touches the rotor and the
spring is lightly pressed (about 0.5 mm).
4. Save the positioning value.
6.5.1.3. Adjusting the arm positioning

In this screen (Figure 6.11) the position is adjusted in terms of polar direction, height and positioning in the rotor
of the five arms: sample arm, reagent 1 arm, reagent 2 arm and the arms of stirrers 1 and 2.
The screen shows a tab for each type of arm (1).
Figure 6.11 Screen for adjusting the arm positioning
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Each tab contains a table with the positions that can be adjusted for each arm (2).
Depending on each position, the necessary adjustment boxes (4) will be activated for moving the selected element
step by step.
With respect to the 3 possible values to be changed in each position: Vertical, Z and rotor, not all can be memorised
as adjustments. Some can be changed to facilitate the adjustment, but not memorised. The top of the table is marked
in yellow, to indicate which parameters are adjustments and are memorised.
After making and saving the adjustments, an OK tick will appear at the side of the element and then the next ele-
ment is automatically accessed.
It is also possible to verify the positioning of each mechanical element in this screen. To do this, press the Checkbut-
ton (3). The selected element will be moved to the adjusted position.
Then the different positions that can be adjusted, depending on the arm, are displayed.
Sample arm
Adjustment position Description
Dispensation Adjustment of the dispensation point in the reagent rotor.
Predilution Adjustment of the dispensation point in the reagent rotor predilution well.
Z Ref. Adjustment of the reference position for lowering the tip in the reaction rotor. To
facilitate the adjustment, the reference is the rotor surface.
))
See Figure 6.12
Washing Adjusting the position of the tip in the wash station.
Ring 1 Adjusting the position of the arm in ring 1 of the sample rotor. The vertical angle
of the arm, arm depth and rotor angle are adjusted. The depth is adjusted for each
sample well.
sample well.
sample well.
Z tube Adjusting the depth of the tubes. This adjustment is used for the 3 rotor rings.
ISE position Adjusting of the dispensation tip when the sample enters the ISE module.
Parking Adjustment of the arm parking positioning.
Reagent 1 and 2 arms

Dispensation Adjustment of the dispensation point in the reagent rotor.
))
See Figure 6.12
Washing Adjusting the position of the tip in the wash station.
Ring 1 Adjusting the position of the arm in ring 1 of the reagent rotor. The vertical angle of
the arm, arm depth and rotor angle are adjusted.
Ring 2 Adjusting the position of the arm in ring 2 of the reagent rotor. The vertical angle of
the arm, arm depth and rotor angle are adjusted.
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Reagent 1 and 2 arms
Dispensation Adjustment of the stirring point in the reaction rotor.
))
See Figure 6.12
Washing Adjusting the position of the stirrer in the wash station.
Icon appearing when stirring arms 1 and 2 are selected. When it is pressed the stirring rotation is activated and
deactivated. In this way, it can be verified whether the stirrer is touching the rotor while in operation.
Figure 6.12 Z ref. adjustment point
6.5.2. Photometry
This menu is used to verify the led currents and for photometric verifications. The currents are adjusted every time
a baseline is performed.
6.5.2.1. Baseline and darkness current

This screen is used to adjust the currents for each led. See Figure 6.13.
To make the adjustment, a well must be filled with distilled water. The well can be filled automatically by the ana-
lyser or manually by the user.
1. To do this, select the desired option (1) and choose the number of the well to be filled.
2. Press the icon for raising or lowering the wash station in order to remove or insert a rotor.
3. Press the adjust button to start the current adjustment process for each led. This is an automatic process that
changes the led current, using both photodiodes (main and reference) to make the reading. The objective is to
ensure that the number of counts of the photodiode with the most light succeed is close to 900,000 without
the other photodiode becoming saturated.
4. After making the automatic adjustment, the results are displayed. The required currents for each wavelength
are shown in the position (2) on the screen. All values outside the pre-established ranges will be marked with
a warning symbol.
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5. A bar chart is shown in position (3) of the screen with the numerical values of the results for the number of
counts obtained for the main photodiode and the reference photodiode.
6. All values outside the pre-established ranges will be marked with a warning symbol.
7. The darkness current values for the main and reference photodiodes are shown in position (4) of the screen.
The darkness current is the current ready by the photodiode when there is no light.
Figure 6.13 Current adjustment screen
8. Press the edit button to access the screen for memorising the reference currents. See Figure 6.14.
9. The reference currents are current values memorised initially and they are used for comparison with the values
obtained in each baseline. This way, the changes in luminous intensity for each led can be determined. This
comparison process is automatic and the user is only warned if the compared values are widely diverging.
10. Due to the long life of the leds and the “Hard Coating” filters, a warning is given only in the event of a failure.
If a led or filter is damaged.
11. In the event of a failure, meaning that a filter or led must be replaced, the technician must memorise the refer-
ence current again.
12. Select the wavelength on which the operation was performed and press save.
Figure 6.14 Screen for memorising the reference currents.
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6.5.2.2. Metrology
This option is used to check the analyser photometry status. See Figure 6.15.
The following checks can be made:
• Repeatability of the readings.
• Stability of the readings.
• Absorbance reading.
Figure 6.15 Photometric verification screen.
To make a verification the rotor must be filled with distilled water. This can be performed by the analyser or the
user can do it manually.
1. Select the rotor filling mode and the well where the readings will be made.
2. Press the icon for raising or lowering the wash station in order to remove or insert a rotor.
3. If you want to measure the absorbance of a specific reagent or fluid, select the manual filling option. Dispense
the fluid to be measured into a well. In the screen, select the well where the fluid was dispensed.
4. Select the type of measurement to be made.
5. Press the adjustment button to start the measuring process.
Repeatability
This verification takes measurements in all the wavelengths for 3 minutes. It is used to verify the repeatability of
the photometric system readings.
After taking the measurements it shows the following parameters for each wavelength:
• Mean
• Standard deviation of all measurements
• Coefficient of variation (CV)
• Maximum absorbance
• Minimum absorbance
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))
See chapter AV ranges accepted for parameters.
Stability
This verification takes measurements in all the wavelengths for 30 minutes. It is used to verify the stability of the
photometric readings
After taking the measurements it shows the following parameters for each wavelength:
• Mean
• Standard deviation of all measurements
• Coefficient of variation
• Maximum absorbance
• Minimum absorbance
Absorbance reading
This allows you to measure the absorbance of a selected well for all wavelengths.
Select the wavelength in the selection chart to display the absorbance result.
If the result gives the value -1 this means that the absorbance measurement has exceeded the photometric range limit.
6.5.3. Scales, bottles and tanks
6.5.3.1. Scale adjustment for determining level.

The levels of the washing solution and high contamination waste bottles are determined by weighing. To adjust the
scales, proceed as follows:
Washing solution bottle adjustment

1. Put the full washing solution bottle in place.
2. Press the FULL adjustment button.
3. Put the empty washing solution bottle in place.
4. Press the EMPTY adjustment button.
5. Save the results.
High contamination waste bottle adjustment

1. Put the full waste bottle in position.
2. Press the FULL adjustment button.
3. Put the empty waste bottle in position.
4. Press the EMPTY adjustment button.
5. Save the results.
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Final verification of the washing solution and high contamination waste bottle levels
To verify whether the bottle level adjustment is correct, place a known level of fluid in the washing solution or waste
bottle and check that the value shown on the screen (see Figure 6.16) inside the bottles matches the real bottle level.
Figure 6.16 Scales adjustment screen
6.5.4. Verification of the dispensation pumps, pumps and valves.

This screen is used to verify the fluidic status of the analyser. The entire fluidic circuit is divided up into functional
parts. Each part can be checked with the same buttons.
Figure 6.17 Screen for checking the pumps and electro valves
1. Select one of the circuits to be checked (1):

• Internal dosing circuit
• External washing circuit
• Wash station aspiration
• Wash station dispensation
• Fluid input/output
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2. Select whether you want to perform one cycle or continuous cycles (2).
3. Press the electro valve you want to activate. It will change from grey to green, indicating it is active (3).
4. Press the pump you want to operate, it will change from grey to green (4).
5. Enter the number of steps you want the dispensation pumps to perform in the adjustment box.
6. Check in the analyser that the fluids enter and leave, depending on the electro valves and pumps that are activated.
7. On exiting the test, all the electro valves and pumps that are active will be deactivated.
8. In circuits in which a pump and an electro valve are next to each other, when the pump is activated the electro
valve will always be activated and when the electro valve is deactivated, the pump will be deactivated.
6.5.5. Adjusting the thermostatting systems

Screen for adjusting the thermostatting of the reaction rotor, the reagent tip assembly and for thermostatting the
wash station.
6.5.5.1. Wash station thermostatting adjustment

The distilled water or washing solution dispensed by the wash station are thermostatted beforehand, to prevent
interference with the rotor temperature. The thermostatting adjustment is made in this screen.
))
See Figure 6.18.
Follow the steps to execute the adjustment process:
1. Press the adjustment button of section 1 to execute a fluidic conditioning of the system.
2. Remove the rear cover and place the thermometer sensor in the heater measuring point.
))
See Figure 6.19.
3. Enter the value of the temperature measured with the thermometer in the box of section 2.
4. Press the adjustment button of section 3 to change the regulation system password if the value measured is out
of the ranges.
))
Consult chapter AV to see the ranges accepted in making the wash station heater adjustment.
5. Save the adjustment value.
Figure 6.18 Wash station thermostatting adjustment screen
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Figure 6.19 Point for measuring the wash station heater temperature
6.5.5.2. Adjusting the reaction rotor thermostatting

To perform the sample and reagent reactions correctly, the reaction rotor must be at a stable temperature. The reac-
tion rotor thermostatting adjustment is made in this screen.
))
See Figure 6.20.
1. Press the adjustment button of section 1 to execute a fluidic conditioning of the system. This conditioning
operation takes about 5 minutes. The rotor can be filled automatically or manually.
2. Place the thermometer sensor in each of the 4 reaction rotor measuring points.
))
Consult Figure 6.21 to see the measuring points.
3. Enter the vale of the temperature measured with the thermometer at each point in each of the boxes of section 2.
of the ranges.
))
Consult AV to see the ranges accepted in adjusting the reaction rotor.
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Figure 6.20 Rotor thermostatting adjustment screen
Figure 6.21 Reaction rotor measuring points
6.5.5.3. Adjusting the tip thermostatting

Reagent tips R1 and R2 suction the reagents from the fridge. Before dispensing the reagent in the rotor, it is ther-
mostatted to a temperature that is closer to that of the rotor. The thermostatting of each arm is adjusted in this screen.
))
See Figure 6.22.
1. Select the arm in which the adjustment is to be made.
2. Press the adjustment button of section 2 to execute a fluidic conditioning of the tip.
3. Place the temperature measuring tool and thermometer sensor in the tip wash station.
))
See Figure 6.23.
4. Press the adjustment button of section 3 for the analyser to dispense a volume of water in the wash station.
5. Enter the value of the temperature measured with the thermometer in the box of section 3. Perform several
dispensing cycles to check that the temperature remains stable.
of the ranges.
))
Consult chapter AV to see the acceptance ranges in adjusting the tip thermostatting.
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Figure 6.22 Screen for adjusting the tip thermostatting
Figure 6.23 Tool for adjusting the tip thermostatting
6.5.6. Barcode reader adjustment

This screen allows you to adjust the positioning of the barcode reader.
))
See Figure 6.24.
1. First select the barcode reader you want to adjust.
2. Remove the cover and place a tube or bottle with a barcode in position 1 of the rotor, to obtain the reference
of the beam to be adjusted.
3. Move the rotor step by step with the adjustment box until the reader beam is properly centred on the barcode.
4. Save the adjusted value.
5. Check in section 3 that the reader receives the readings correctly. The indicated value must be higher than 95%.
6. To perform the barcode reader test, place several tubes or bottles in the chosen reader rotor and press the test but-
ton of section 4. The table will show the barcodes read and the positions in which the tubes/bottles were placed.
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Figure 6.24 Barcode reader adjustment screen
6.5.7. ISE module

This menu is used to launch the different maintenance actions required for the ISE module.
The following functions can be executed:
• Calibrate
• Install a reagent kit
• Install the electrodes
• Deactivate the module for a long period of time
• Change the peristaltic pump tubes
• Activate the ISE preparations
For each function several actions must be executed. Select one of the functions and show the set of actions to be
executed step by step.
))
See the explanation of each step in detail in chapter 14.2.2 of the user manual.
Select an action and press the execute button. Information about the action will appear in the results zone. It will
say whether the action was successfully completed (the text is shown in black) or has errors (the text is shown in
red). The results are shown in the actions that return information, such as calibrations.
In addition each of the actions is positioned in a group under the name General; if the user only wants to perform
one of the actions, that user can launch it directly.
Action Description
Maintenance Empties the tubes. Only activates the waste pump. In
the repetitions parameter indicate how many times the
action must be executed.
Bleed A Performs a priming cycle with calibration standard A
using a volume of 100 μL. In the repetitions parameter
indicate how many times the action must be executed.
Bleed B Performs a priming cycle with calibration standard B
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Action Description
Priming A Performs a priming cycle with calibration standard A
Priming B Performs a priming cycle with calibration standard B
Wash Performs a wash cycle with the ISE washing solution.
In the sample rotor pos. parameter indicate the position
of the tube with the washing solution. In the volume
parameter indicate the volume to be dispensed for
washing.
Activate the reagent Execute this action to activate and memorise the reagent
kit kit in the programme. It is also used to memorise the
installation date and record the consumption of the
calibration standards. The program issues a warning
when the standards are no longer usable.
Activation of Execute this action to activate and memorise the
electrodes electrodes in the programme. It is used to record the
consumption of the electrodes and warn the user when
they are no longer usable.
Activation of ISE Use this action to tell the programme you have installed
preparations an ISE module.
6.5.8. Stress
The stress verification test simulates the normal operating cycle of the analyser. However, instead of pipetting serum
and reagent, the analyser moves the arms and pumps, but without pipetting any fluids.
The stress can be configured as global stress, i.e., performing a complete cycle or partial stress of functional parts
of the analyser.
Partial stress options:
• Arms (selected separately)
• Rotors (selected separately)
• Photometry
• Syringes
• Fluids
Steps for programming stress
1. Select the stress duration by number of cycles.
2. Select whether you want the global or partial stress option.
3. Press the adjustment button to start the stress test.
4. While the stress test is being executed, a status bar will appear indicating the stress duration.
5. Once the stress test has finished, the following information is displayed in the results section:
a) Type of stress.
b) Total number of cycles and total stress duration time.
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c) Number of cycles completed.

d) Number of restarts and cycle in which the restart was executed. This serves to verify whether a power cut
occurred in the stress with very long durations.
e) Number of errors and description of the error. After a certain number of cycles the analyser performs
an internal check to detect whether there are any problems in its operation. For instance, it counts the
number of steps in each motor to check that no step has been lost. If any problem is detected, this is
shown in the error box.
Figure 6.25 Stress screen
6.6. Utilities
6.6.1. Demo mode

Enables the analyser to be put in demo mode. This mode performs the basic pipetting and dispensation cycle, which
entails a movement of the arms and rotors but no processing of fluids or photometric readings.
Press the adjustment button to start the demo mode.
The analyser will continue performing the demo mode until the stop button is pressed.
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Figure 6.26 Demo mode screen
6.6.2. Analyser information

This screen allows you to input or change the analyser serial number.
Press the edit button to change the serial number.
It also performs the same verification process as the one performed by the analyser when being initialised.
Press the “show details” button to see the different steps executed in the verification process.
Figure 6.27 Analyser information screen
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6.7. Recording and maintenance
6.7.1. Historical reports

This screen is used to consult all the actions performed with the service programme.
All the actions performed are memorised in a database. To consult the actions, select the filter fields and press search.
Filter Description
Analyser serial Enter the serial number of the analyser for which the
number actions are to be consulted.
Date Enter the date range through which you want to filter
the actions.
Tasks Select one of the possible tasks: All, Adjustments, test
or utilities.
Actions A list of actions will appear, depending on the chosen
task. In the event that many actions have been
performed, it is used to view only the chosen action.
After selecting the filter fields, the programme displays a table with the filtered information.
The user can enter text in the comments column to make a remark about the selection action. Click the inside of
the comments box to enter the text. When you have finished, press the save or undo button.
IF you want to delete a historical action, select the action and press the delete button.
Figure 6.28 Historical information screen
6.7.2. TAS report

This screen allows you to write a report for the technical service.
Press the “save TAS report” button and a copy of the historical action database will be made automatically.
The file generated is located in the following folder:
c:\Program Files (x86)\BA400\BA400 Service\SATReports\
It also allows you to delete old TAS Reports.
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6.8. Exit
To exit the programme go to the exit menu and select one of the two options:
• Exit by switching off the analyser: This option closes the programme and tells the analyser to switch itself
off and complete the closing process.
• Exit without switching off the analyser: This option will only close the programme and leave the analyser
on and on standby.
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7. Maintenance and cleaning

7.1. Maintenance activities and frequency
Operations to be performed by the instrument operator.
Activities at the start of the day

1 Replenish the washing solution bottle
2 Initialise the analyser. Perform the warm-up with the programme
3 Execute 2 conditioning cycles
4 Check the temperature of the reaction rotor and the reagent rotor
5 Check the reagent volume
6 Calibrate the ISE module peristaltic pumps
7 Calibrate the ISE electrodes
Activities at the end of the day

1 Wash the ISE module
2 Switch off the analyser by executing the Shut-down with the programme
3 Empty the high contamination bottle
4 Remove the calibration solutions, controls and samples from the sample rotor
Activities to be executed every week

1 Change the reaction rotor
2 Calibrate the ISE module air bubble detector
3 Clean the ISE module inlet cup with a cotton bud
4 Clean the working surface
5 Clean the interior of the reagent rotor vessel
6 Clean the interior of the sample rotor vessel
7 Check the capacity of the ISE module reagent pack and replace it, if used up
8 Rinse the ISE module waste tubes with distilled water
9 Clean the stirrer paddles with a cloth soaked in washing solution
Operations to be performed by the technical service.
Six-monthly Maintenance
1 Change the silicone tubes of the peristaltic pumps in the ISE module
2 Change the electrodes in the ISE module
3 Check or change the external 150 µm filter of the distilled water inlet
4 Check or change the 150 µm filter of the washing solution bottle
5 Dust the electronic board and fans
6 Check the internal fluidic connections and make sure none of them leaks
7 Clean the inner containers of distilled water and low concentration waste
8 Clean the exteriors of the tips of the arms, the stirrers and the wash station tips
9 Clean the barcode reader window
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Operations to be performed by the technical service.
Annual Maintenance
1 Change the Teflon tubes of the ISE module
2 Change the degasser membrane
3 Check the wash station piston pump (check for leaks and grease the bearings)
4 Check the 5 arms and the wash station (check the tension and wear of the belts)
5 Check the anti-collision system of the arms and the wash station
6 Check the friction of the stirrers in the reaction rotor
7 Check the wear and tension of the belts in the sample, reagent and reaction rotors
8 Check the position and sensor of the reagent, sample and reaction covers
9 Check the adjustment and positioning of the sample and reagent rotors
10 Check the rotation of the fans
11 Check that the analyser levelling is correct
12 Check that the general cover is correctly supported and cannot fall
7.2. Cleaning
Material and tools needed to clean the appliance:
• Dry air aerosol container or air blower
• T20 torx key with a minimum length of 40 mm
7.2.1. Cleaning the interior compartments

Every time an operation is performed with the analyser, it is advisable to clean the electronic and fluidic compartments.
1. Remove the side housings.
2. Remove the side panels.
3. Blow the dust from the electronic boards, components and fans with dry air.
7.2.2. Checking the fluidic connections

Check that all the fluidic connections and connections to the electro valves and pumps have no leaks.
2. Remove the side panels.
3. Touch each connection with your hand to verify that the joints are not leaking. Check that the fittings are
tightened at a torque of 33 CN.
4. Use the service programme to open and close the electro valves and activate the pumps. Ensure there are no
fluid leaks in each element when in both states.
7.2.3. Cleaning the water and low contamination waste containers

1. Remove the rear cover.
2. Remove the clamps securing the distilled water and low contamination waste bottles.
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3. Empty the fluid system using the service programme.

4. Unscrew each bottle.
5. Wash the distilled water and low contamination waste containers with neutral soap and water.
6. Check and replace the water inlet filters in each container.
7.2.4. Washing the outside of the tips

Wear gloves and protective clothing when handling the tips.
With the analyser off, lift the 3 tips, 2 stirrers and wash station manually.
Take care when lifting the tips.
1. Clean each tip, stirrer and the wash station tips with a cloth soaked in washing solution.
2. Repeat the process with 70 alcohol.
3. Initialise the analyser with the service programme and wash the tips using the washing utility.
4. Check that the wash station dryer is correctly secured and in good condition. If it is damaged, replace it with
a new one.
7.2.5. Cleaning the barcode reader window

Wash the inside and outside of the barcode reader window.
1. Remove the top panel of the analyser.
2. Use a damp cloth to clean the inside of the two barcode reader windows.
3. Remove the two rotors from their compartments.
4. Clean the outside of the two barcode reader windows with a damp cloth.
7.2.6. Wash the heating canal and the canal of the rotor containers
Use a damp cloth and neutral soap.
1. With the analyser switched off, lift the wash station manually.
2. Remove the covers from the 3 rotors.
3. Remove the PMMA rotor and the sample and reagent rotors.
4. Wipe the surface of the heating canal and the inside of the rotor vessel with a cloth.
5. Check that the drains are not blocked. To do this, run some water through the opening and check that the
water disappears down the drain.
7.3. Maintenance
All maintenance work will be performed every 2 years or when an operation has been performed by the technical
service.
Material and tools needed to perform maintenance on the appliance:
• T20 torx key with a minimum length of 40 mm
• Set of Allen keys
• SAE-40 or any equivalent lubricant
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7.3.1. Checking of the wash station piston pump
1. Remove the housing on the right side.
2. Remove the panel on the right side.
3. Touch the fluidic connections of the 5 PMMA chambers and ensure there are no leaks.
4. Apply 2 drops of SAE40 lubricant to the linear bearings of the pump.
7.3.2. Checking of the reagent and sample arms, stirrers and wash station
Check the tension of the belts:
5. Remove the top panel of the analyser.
6. Check the tension of the two belts of each arm (the 2 reagent arms, sample arm and the two stirrers). The belt
tension must not be too loose or too tight.
7. Also check that the belt is not worn and that there are no traces of material adhering to the cogs.
Check that the anti-collision system of the reagent and sample arms is operating correctly:
1. Remove the top housings from the 3 arms.
2. Initialise the analyser with the service programme.
3. Go to the anti-collision system checking option.
4. Lift each tip with your hand and check that they draw back smoothly and correctly.
5. Check that the lamp on the panel goes on every time the tip is in the raised position.
6. Check the indication that the tip is in the raised position in the service programme.
Check that the stirrers function correctly:

1. Check that the stirrer blades are properly tightened. Take care when tightening the blades, to ensure they con-
tinue to be aligned with respect to the motor shaft.
2. Use the service programme to rotate the stirrers. Check that they rotate correctly and are not eccentric.
Check the anti-collision system in the wash station

1. Remove the top housing from the wash station.
3. Go to the wash station anti-collision system checking option.
4. Lift the dryer tip with your hand and check that it draws back smoothly and correctly.
5. Check the indication that the dryer tip is in the raised position in the service programme.
6. Lift the wash station pedal system with your hand and check that it slides evenly and returns smoothly to its
original position.
7.3.3. Checking the sample and reagent rotors

Check the rotor covers:
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Service manual
2. Check that the cover sensor functions correctly.

3. Check that the covers fit into their housing correctly.
Check that the rotors fit into their compartments correctly:

1. Remove the two covers from the rotor assemblies.
2. Check that the anchoring of the sample and reagent rotor shaft functions correctly. If it is in position, it must
not come out unless the top button is pushed and when inserting it, it must glide in smoothly.
Check the fans:

1. Check that there is no fridge or reaction rotor fan operation alarm.
7.3.4. Inspect the cover and the structure

1. Check that the general fans are functioning correctly. Put your hand on the exterior to verify that they expel
air. The general fans have no automatic operation detection system.
2. Check that the wheels have no defects.
3. Check that the analyser is correctly positioned, and that the legs make contact with the floor. Tighten them
with a spanner.
4. Check that the analyser is perfectly level with a levelling instrument. Tighten or loosen the legs until the analyser
is completely level.
Checking the cover and legs:

1. Check that the doors open and close properly and smoothly. Check that they are correctly aligned with each other.
2. Check that the main cover is correctly supported when fully open and that when closing it, it does not fall
suddenly.
3. Apply 1 drop of SAE-40 to the rear hinges of the main cover. Open and close the cover several times to help
distribute the oil in the hinge.
4. Check the state of the “bumpers” (rubber stops for preventing the main cover from colliding), and if they are
worn or damaged, replace them.
7.3.5. ISE module inspection

Replace the peristaltic pump tubes every 6 months.
Clean the ISE module sample entry opening.
Check that the tubes are not blocked.
114
Installation registration
sheet
Laboratory name:
Analyser model:
Serial number:
Name of technical director:
Date of installation:
Steps Actions carried out by the technical service Done

1 Location/Anchoring of analyser/Levelling
2 Installing the high contamination waste and washing solution containers
3 Preparing the washing solution
4 Purified water connection
5 Low contamination waste connection
6 Installing the sample and reagent rotors
7 Installing the reaction rotor
8 Connection to the electricity grid
9 Connecting the communication cable to the computer
10 Installing the user programme in the computer
11 Configure programmes in second plane
12 Initialise the analyser
13 Check that each pump functions correctly. Internal washing of tips. External
washing of tips and stirrers. If necessary, check it by running the service programme.
14 ISE module installation
Actions carried out by the product specialist
14 Install reagents
15 Programme the calibration values and control margins
16 Initialise the ISE module electrodes
17 Make a worklist of blanks, standards and controls for the tests requested by the
client. Check that the results are within the margins. (Take note of the results on the
attached sheet)
Signature and date
115
Service manual
Installation registration
sheet
Control I Control II
Test Batch Result Tolerance Batch Result Tolerance
Signature and date
116
Preventive maintenance
record
Laboratory name:
Analyser model:
Serial number:
Name of technical director:
1st 2nd 3rd 4th

Steps Actions carried out by the technical service
Quarter Quarter Quarter Quarter
1 Change the silicone tubes of the peristaltic pumps
in the ISE module
2 Change the electrodes in the ISE module
3 Check or change the external 150 µm filter of the
distilled water inlet
4 Check or change the 150 µm filter of the washing
solution bottle
5 Dust the electronic board and fans
6 Check the internal fluidic connections
7 Clean the inner containers of distilled water and
low concentration waste
8 Clean the exteriors of the tips of the arms, the
stirrers and the wash station tips
9 Wash the barcode reader window (reagent and
sample rotor)
10 Change the Teflon tubes of the ISE module
11 Change the degasser membrane
12 Check the wash station piston pump
13 Check the 5 arms and the wash station (belts and
tension)
14 Check the anti-collision system of the arms and
the wash station
15 Check the friction of the stirrers in the reaction
rotor
16 Check the belt and tension in the sample, reagent
and reaction rotors
17 Check the positioning of the sensor in the
reagent, sample and reaction covers
18 Check the adjustment and positioning of the
sample and reagent rotors
19 Check the rotation of the fans
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Service manual
1st 2nd 3rd 4th

Steps Actions carried out by the technical service
Quarter Quarter Quarter Quarter
20 Check that the wheels have no faults and that the
analyser levelling is correct
21 Check that the general cover is correctly
supported and cannot fall.
118
8. Description of how to dismantle subassem-
blies and spares
8.1. Dismantling of housing
The main cover, side housings, doors and rear panel can be removed separately, without having to dismantle the others.
To dismantle all the housings and panels, only one tool is required:
T20 torx key with a minimum length of 40 mm.
To dismantle the housings and panels follow the dismantling process in reverse.
At the time of mounting the covers, if an automatic screw tightening is used, use the control torque
(2.5Nm). Never use the option drill.
NOTE
8.1.1. Removing the rear cover
To remove the rear cover, proceed as follows:
1. Remove the 14 torx screws and their washers (3).
2. Grasp the rear panel (2) using the 2 grips (1).
Figure 8.1 Removing the rear cover
8.1.2. Removing the top cover

To remove the top cover, proceed as follows:
1. Open the cover and remove the cover sensor tab (5) to prevent it from being damaged, and the 2 pistons that
support the cover. To take out the pistons, remove the pincer that holds the head in place through the top part (4).
2. Close the cover and turn the analyser.
3. Remove the 10 torx screws (6) and their washers from the hinges.
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Service manual
4. The cover can now be raised and changed.
Figure 8.2 Dismantling the piston
Figure 8.3 Dismantling the cover screws
8.1.3. Removing the side housings

To remove the housing on the right side, proceed as follows:
1. Remove the 3 screws from the side of the top tray (1).
2. Open the right door and remove the 2 internal screws (2).
3. Remove the 3 external screws (3).
120
4. Lift the housing slightly and remove the side from the support.
Proceed in the same way to remove the housing on the left side.
Figure 8.4 Dismantling the side
Figure 8.5 Dismantling the side
8.1.4. Dismantling and assembling the ISE cover

To remove the ISE cover, proceed as follows:
1. Open the ISE cover (1) to access the 2 screws that hold it in place (2).
2. Using an M4 Allen key, remove the 2 screws (2) together with their nuts, washers and the divider.
3. Remove the ISE cover.
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Service manual
Figure 8.6 Removing the ISE cover
Proceed as follows to assembling the ISE cover (see Figure 8.7):

1. Assemble the screw, washers, nuts and divider in the cover before reassembling it again on the analyser.
2. Mount both assemblies as shown in Figure 8.7, on each side of the ISE cover.
3. Do not tighten the nuts.
4. Position the ISE cover on the analyser and slightly tighten the screws (1) on each side.
5. Tighten one screw or the other until the ISE cover is correctly centred.
6. Secure the screw with the Allen key and tighten the nut with a Ford key (3).
7. Repeat the process with the nut (4).
1 2 2 3 4 5
Figure 8.7 Detail of installing the ISE cover
122
8.1.5. Remove the top front housing
The top front housing has two parts. Proceed as follows to remove one from the other.
1. Remove the ISE cover.
))
See how to remove the ISE cover in chapter 8.1.4
2. Remove the left side (to remove the left front part).
))
See how to remove the left side in chapter 8.1.3
3. Remove the 4 screws from the top tray (1).
4. Remove the 2 internal screws (2).
5. Remove the housing. Before removing the housing make sure you have first removed the LED indicator cable.
6. To reassemble the housing, follow the above steps in reverse order. First assembly the LED indicator cable.
Figure 8.8 Dismantling the front
8.1.6. Dismantling the top tray

1. Lift all the arms and the wash station manually.
2. Remove the plastic protective caps (1) from the base of the arms.
3. Remove the plastic cap from the base of the stirrers (4).
4. Remove all the screws (2) used to anchor the cover.
5. Lower the arms manually to the lowest level.
6. Remove the top cover(3). Take care in handling it, as it is very large.
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Service manual
1 2 3
Figure 8.9 Dismantling the top tray
8.2. Removing the sample and reagent arms and the stirrers
Figure 8.10 Removing the arm
1. Remove the top cover.

))
See chapter 8.1.6
2. Remove the rear cover, to access the interior cables.
))
See chapter 8.1.1
3. Remove the clamps used to secure the cables and tubes that emerge from the lower part of the arm that you
want to dismantle.
4. Remove the 3 screws (1) used to anchor the arm assembly. Use a screwdriver and lengthener to access the screws.
8.3. Dismantling the reagent rotor

))
See chapter 8.1.6
2. Unplug the 5 fans connected to the CIIM00052 board.
124
3. Unplug the two CAN cables connected to the CIIM00052 board.
4. Remove the reagent 2 arm, to leave sufficient space for taking out the rotor.
))
See chapter 8.2
5. Unscrew the 5 screws anchoring the rotor assembly to the base. See Figure 8.11.
6. Remove the rotor assembly and follow the steps set out in Figure 8.12 to take the rotor out of the analyser.
Figure 8.11 Rotor anchoring screws
Figure 8.12 Steps required to take the rotor out of the analyser
8.4. Dismantling the sample rotor

))
See chapter 8.1.6
2. Unplug the 2 fans connected to the CIIM00052 board.
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Service manual
3. Unplug the CAN cable.

4. Remove the sample arm.
))
See chapter 8.2
5. Unscrew the 4 screws anchoring the rotor assembly to the base. See Figure 8.13.
6. Unscrew the screw that secures the wash station housing.
7. Disconnect all the wash station tubes and take them from the housing.
8. Remove the rotor assembly and follow the steps set out in Figure 8.14 to take the rotor out of the analyser.
8.5. Dismantling the reaction rotor

))
See chapter 8.1.6
126
2. Unplug the CAN cable.
3. Remove the stirrer 2 arm.
))
See chapter 8.2
4. Remove the sample rotor.
))
See chapter 8.4
5. Unscrew the 5 screws of the Figure 8.15.
6. Take the rotor assembly out of the analyser .
Figure 8.15 Reaction rotor anchoring screws
8.6. ISE module installation

The ISE module is optional. To install the ISE module, proceed as follows:
The spares content is as follows:
Component Description
Power supply
Switch
Cabling
Cable gland
Connector and tubes
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Service manual
Component Description
ISE module

2. Remove the top cover. Remove from the cover the opening plug for dispensing the ISE module sample(8). See
Figure 8.18.
3. Dismantle the top front housings together with the ISE cover (7).
4. Dismantle the switch assembly (2). See Figure 8.16.
5. Remove the switch cover (1).
6. Insert the ISE switch.
7. Connect the power cable to the switch.
Close the switch assembly with the protective cover (3) and put it back in place. See Figure 8.17
1 2 3
Figure 8.16 Dismantling the switch assembly
8. Lower the cabling through the analyser column (4). See Figure 8.17.
9. Install the power supply (5) as shown in the previous figure.
10. Connect one of the cable ends to the power supply.
11. Connect the other end to the AC distribution board (6).
12. Connect the module power cables to the power supply output. These cables are already installed in the analyser.
128
13. Insert the module in its compartment. See Figure 8.19. Use the same screws that secure the cover to screw the
module in place.
14. Place the pincer (9) of the calibration standard kit in the compartment located next to the internal bottles. See
Figure 8.20.
15. Pass the tubes through the two openings (10) and (11) until they reach the module.
16. Consult the user manual chapter on installing the ISE module to see how to connect the different tubes and
install the electrodes and standard kit.
17. Replace all the covers and housings.
18. Install the cable gland in the sample dispensing opening in the top cover.
4 5 6
Figure 8.17 ISE power module installation
7 8
Figure 8.18 Location of the covers
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Service manual
Figure 8.19 ISE module assembly
9 10 11
Figure 8.20 Tube installation
130
8.7. Wash station pump spares
AC16588
AC16587 1
2
AC16585
AC16816 3
AC16817 4
AC16586 5
AC16587 (2)+(3)+(4)+(5)+(6)
AC16585 (3)+(5)
Code Description of the spare

AC16588 Piston pump assembly
AC16587 Upper chamber assembly
AC16816 Piston support and piston assembly
AC16817 Clamp
AC16586 Piston pump motor
AC16585 Stop and O-ring
8.7.1. AC16587 - Top chamber and AC16585 - Stop and O-ring
Tools: 2.5 mm and 3 mm Allen key

Dismantling:
1. Remove the 4 screws (7).
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Service manual
2. Dismantle the top chamber assembly of the pump (8).

4. Remove the stop support (2), the stop (3), the bubble suppressor (4) and the O-ring (5) from the top chamber (6).
Assembly:
1. Replace the faulty part (chamber or stop).
2. Clean the stop support with absorbent paper (2). Lubricate the stop support (2) and the stop (3) with washing
solution to facilitate the assembly operation.
3. Carefully put the stop (3) in the stop support (2) by pressing the stop evenly without deforming the internal
springs. Use a flat element to press the stop.
4. Check that the stop is properly levelled with the stop support.
5. Install each element in the order shown in Figure 8.21. Check that they are all correctly positioned in a con-
centric layout.
6. Put the 4 screws in place (1) to anchor the stop assembly firmly in the chamber.
7. Wet the piston head and interior of the stop with washing solution.
8. Carefully insert the chamber and stop in the piston.
9. Put the 4 screws in place (7) to anchor the chamber to the pump.
Figure 8.21 Installing the stop
8.7.2. AC16816 - Piston support assembly and pistons

Tools: 2.5 mm and 3 mm Allen key
132
Dismantling:
1. Remove the 5 chambers (9).
))
See AC16587 - Top chamber
2. Remove the clamping device by taking out the screws (10) and (11).
3. Loosen the screws (12) to release the motor from the rack.
4. Remove the screw (13) to take out the rack through the top.
5. Remove the piston support with the pistons.
10
11
12
13
Figure 8.22 Piston support assembly
Assembly:
1. Insert the new piston support.
2. Insert the rack and tighten the screw (13).
3. Tighten the motor screws (12). Check that the movement of the gear assembly with the rack is smooth.
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Service manual
4. Install the chambers one by one, and put the screws in place (7) without tightening them. The pistons must
be half way inside and halfway outside the chamber to ensure the correct alignment of the pistons with the
chamber. Once the screws of all the chambers are in position, insert the pistons inside the chambers and tighten
the screws crosswise.
5. Move the rack to the bottom position.
6. Insert the clamping device and put the screws in place (10) and (11) without tightening them.
7. Place the piston support at a distance of 15.4 mm from the part that anchors the motor (14).
8. Tighten the screws in this order, first (11) and then (10).
9. Check the up and down movement of the pistons.
8.7.3. AC16817 - Clamping device

Tools: 2.5 Allen Key
Dismantling:
1. Remove the clamping device by taking out the screws (10) and (11).
Assembly:
1. Insert the new clamping device.
8.7.4. AC16586 - Pump Motor

Tools: 3 mm Allen key and 5.5 Ford key
Dismantling:
1. Remove the 4 screws that anchor the motor (12).
Assembly:
1. Replace the motor.
2. Position the screws without tightening them to hold the motor in place.
7. Check the up and down movement of the pistons.
134
14
Figure 8.23 Installing the piston pump motor
8.8. Replacing the degasser
AC17220
Code Description of the codes
AC17094 Degasser
AC17092 Spare degasser membrane
AC17220 Degasser control board
AC17094
AC17092
8.8.1. AC17094 - Installing the degasser module

Assembly:
1. Put the degasser in place (4).
2. Screw it to the base. If there are no holes in the bottom board, use double-sided tape to secure the degasser
module (4).
3. Remove the tube (1) connecting the “Water In” to the premanifold.
4. Connect the tubes at the inlet (2) and outlet (3) of the degasser membrane.
5. Connect the cable that comes with the module to the CN5 connector of the module electronic board (5).
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Service manual
6. Pass the cable through the interior of the analyser to the power sources.
7. Connect the other end of the cable to the analyser power source as follows (6) and (7):
Cable colours Power supply

White Positive terminal
Brown Negative terminal
8. The pressure regulator is already programmed. It must not be touched.

9. Start up the analyser and the degasser vacuum pump should operate for a few seconds to create the vacuum in
the membrane.
3
1
Figure 8.24 Installing the degasser in a machine prior to the change in the ceramic pump design
Figure 8.25 Installing the degasser in a machine with the new ceramic pump design
136
8.8.2. Inserting and removing the tubes from the quick connectors
Inserting a tube into the quick connector:
1. Insert the tube (8) up to the end of the connector.
2. Pull the tube outwards (9) to lock the connection.
Removing the tube from the quick connector:

1. Press the connector flap inwards (10) to release the tube.
2. Pull the tube (11) keeping the flap pressed inwards.
8.8.3. AC17092 - Replacing the degasser membrane

Dismantling:
1. Remove the tubes from both ends of the membrane (12). To remove the tubes, press the outer ring of the con-
nector (10) as you pull the tube (11).
2. Remove the tubes to create the vacuum (13).
3. Remove the vacuum tube adjustment screws (14).
4. Remove the membrane.
Assembly:
1. Insert the new membrane.
2. Position the adjustment screws (14) and tighten them firmly.
3. Insert the tubes to create the vacuum (13).
4. Place the inlet and outlet tubes at the ends of the membrane (12). Check that the tubes are in the correct posi-
tion by pulling them slightly outwards after inserting them.
5. Start up the analyser and the vacuum pump should operate for a few seconds to create the vacuum.
8.8.4. Inserting and removing the tubes from the quick connectors
Inserting a tube into the quick connector:
1. Insert the tube (8) up to the end of the connector.
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Service manual
2. Pull the tube outwards (9) to lock the connection.

Removing the tube from the quick connector:
1. Press the connector flap inwards (10) to release the tube.
2. Pull the tube (11) keeping the flap pressed inwards.
8 9
10
12
11
13
14
138
8.8.5. AC17220 - Replacing the degasser control board
15
16
17
18
Dismantling:
1. Remove the 4 cables (15), (16), (17) and (18) from the degasser board.
2. Remove the board from its housing.
Assembly:
1. Put the new board in position.
2. Connect each cable as follows:
Connector Identification Cable Position

CN2 (15) 3-pin connector ASMB784 or Vacuum switch
ASMB1392
CN3 (17) 2-pin connector ASMB873 Membrane pump
CN4 (18) 2-pin connector Electro valve connection
CN5 (16) 2-pin connector Board power source
8.8.6. Description of how to programme the vacuum switch

1. Plug in the analyser.
)) See Figure 8.27.
2. Press the blue button for 3 s. Display shows: FO (step 1).
3. Press the button (UP). Display shows: FI (step 2).
4. Press blue button, display reads HYS or OVI. Select: HYS (step 3).
5. Press blue button and select: I_P (step 4).
6. Press blue button and adjust with control (UP, DOWN) to 63.0 (step 5).
7. Press blue button.
8. Select: H_I (step 6).
9. Adjust with control (UP, DOWN) to 13.0 (step 7).
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Service manual
Figure 8.26 Description of how the vacuum switch functions
Step 1 Step 2 Step 3 Step 4
Step 5 Step 6
Step 8
Step 7
Figure 8.27 Programming the vacuum switch
140
10. Select with the blue button.
11. Press the blue button for 3 s to exit.
12. Check the functioning of the vacuum system by blocking both membrane outlets and checking that the con-
nection is made at: 65.0 and the disconnection at a: 50.0 (step 8).
8.9. Replacing the arm
AC16538
AC16601
AC16558
AC16602
AC16603
AC16604 AC16599
AC16600
Code Description
AC16538 Arm cover
AC16557 AC16558 Tip board
AC16599 Polar belt
AC16598
AC16557 Arm board
AC16598 Vertical belt
AC16603 Sample tip
AC16604 Reagent tip
AC16600
AC16600 Arm motor
AC16601 Continuous current motor
AC16602 Stirrer paddle
8.9.1. AC16538 - Arm cover

Dismantling: Press both sides of the cover to release the flaps. Remove the cover.
Assembly: Place the housing in position and press downwards until the flaps are inserted in the base.
8.9.2. AC16558 - Tip board

Tools: 2.5 mm Allen key
Adjustment screwdriver (AC16644)
Dismantling:
1. Cut the tip cable holder clamp. Be careful not to damage the board when you cut it.
2. Remove the cables from the tip.
3. Remove the connector from the CAN bus.
4. Remove the 2 screws that fasten the board to the base of the arm.
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Service manual
Assembly:
1. Insert the new board in its correct position and put the 2 screws that hold the board in place, and their nuts.
2. Place the electronic board at a distance of 1 mm from the stop to ensure that the tip board does not cut the
collision sensor.
3. Connect the CAN bus cable.
4. Connect the tip cables as follows:
Connector name HP TP DN
Reagents
Reagents Reagents
Sample
Cable code PLMA0329 PLMA0330 PLMA0328
Cable colour Yellow White Black
5. Put a clamp in place to secure the cables. Check that the cables do not protrude from the top of the electronic
board.
6. Adjust the sensor power meter with the adjustment screwdriver.
7. See chapter 6.5.1 of the service manual.
8. Select the board switch, depending on the arm where it is installed:
Board Code Selector switch

Sample 1 0110
detection
DM1
Reagent detection 1110

DR1
Reagent 2 0001
detection
DR2
9. Check that the tip collision plate enters and emerges correctly from the photosensor. If the plate cuts the pho-
tosensor, raise the sensor plate slightly.
8.9.3. AC16603 - Sample tip / AC16604 - Reagent tip

There are two approved tip models. The difference between them is the diameter of the black encapsulated part at
the top. In one model the encapsulated part diameter is 10 mm and in the other, it is 13 mm.
The spare is supplied with the spring (4) and the fixing spring (2) that is appropriate for the tip.
Black encapsulated part
142
5
1
6
2
7
Figure 8.28 Replacing the tip
Tools: Two 5.5 Ford keys or one 5.5 Ford key and one 5.5. pipe wrench
Dismantling:
1. Cut the cable holder clamp.
2. Remove the cables from the tip.
3. Unscrew the tip tube, holding the tip fitting (5) with one hand and the tube fitting in the other hand.
4. Remove the 2 nuts (1). Grasp the nut with the Ford key (3) and loosen the nut with the other key (1).
5. Remove the 2 safety washers (6).
6. Remove the fixing spring (2).
7. Remove the 2 safety washers (7).
8. Remove the 2 nuts (3).
9. Remove the tip assembly and spring (4).
Assembly:
1. Use the spring (4) and the fixing spring (2) supplied with the spare.
2. Insert the spring into the tip assembly, taking care when passing the connectors through the centre of the spring.
3. Insert the fixing spring into the tip assembly.
4. Put the tip in position.
5. Place the nuts (3) at a height of 12 mm. Then the safety washer (7).
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Service manual
6. Insert the fixing spring (2).

7. Put the other safety washer (6) and nuts (1) in position to hold the fixing spring, blocking the nut movement
(3) with a Ford key.
8. Check that the tip anti-collision system functions correctly.
9. To check the tip anti-collision system: use your finger or a part with a flat surface to simulate a collision with
the tip in a perfectly vertical movement. The spring should rise and then recover on its own, and fall to the rest
position.
10. Connect the tip cables as follows:
Connector name HP TP DN
Cable code PLMA0329 PLMA0330 PLMA0328
Cable colours Yellow/Red White Black
11. Put a clamp in place to fasten the cables to the electronic board. Make sure the cables do not protrude from
the top of the electronic board.
12. Cut the clamp tail.
13. Connect the Teflon tube to the tip. To tighten the fitting, hold the point of the tip (5).
Make sure you tighten the fixing spring nuts firmly, grasping the fixed nut (3) and tightening the
nut (1) on the (3).
WARNING
14. Check that the tip anti-collision system functions correctly. As described in point 9.
15. Check that the LD2 led of the board lights up when it cuts the photosensor and that it goes off when the tip
is in the rest mode.
16. If the collision sensor is very close to the photosensor, raise the electronic board 1 mm from its position.
17. Replace the arm cover.
18. Adjust the tip Zref value with the service programme.
19. Adjust the temperature set point with the service programme, depending on the tip model:
Model R1 R2
Encapsulated part 13 45 °C 45 °C
mm
Encapsulated part 10 55 °C 50 °C
mm
20. Perform the Zref adjustment with the service programme.
))
See chapter 4 for the purpose of making this adjustment.
21. Adjust the temperature of the reagent tips.
144
8.9.4. AC16557 - Arm board
Tools: 2.5 mm Allen key

Dismantling:
1. Remove the cables from the motors and start-up photosensors.
2. Remove the cable from the CAN bus.
3. Remove the 4 screws used to secure the board.
Assembly:
1. Put the board in position.
2. Put the 4 screws used to secure the board.
3. Insert the CAN bus cable.
4. Put the cables from the motors and start-up photosensors.
5. Select the board switch, depending on the arm where the board is installed.

Sample 1 arm 0000
BM1
Reagent 1 arm 1000

BR1
Reagent 2 arm 0100

BR2
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Service manual

Stirrer 1 arm 0010
AG1
Stirrer 2 arm 1010

AG2
8.9.5. AC16599 - Polar belt / AC16600 - Polar motor
10
11
12
Figure 8.29 Installing the polar belt and polar motor
Tools: 2.5 mm and 3 mm Allen key and 5.5 Ford key

))
See Figure 8.29.
Dismantling the motor:
1. Disconnect the motor cable.
2. Remove the 4 screws (10) and (11) used to anchor the motor (12).
Dismantling the belt:
1. Remove the 2 screws (8) from the photosensor.
2. Remove the belt (9).
Assembling the belt:
1. Insert the new belt.
2. Insert the photosensor and its screws (8).
146
3. Adjust the belt tension following the motor assembly instructions.
Assembling the motor:
1. Insert the motor in its housing.
2. Put the 4 screws (10) and (11) and their washers and nuts in position, without tightening them.
3. Install the belt on the motor pulley.
4. Adjust the belt tension to 4 kg, pulling the motor outwards. Start to tighten the screws (11) and then the screws
(10) to secure the motor firmly in place.
5. Connect the motor cable.
6. Perform the arm adjustments with the service programme.
8.9.6. AC16599 - Vertical belt / AC16600 - Vertical motor
13
14
15
16
Figure 8.30 Installing the vertical belt and vertical motor
Tools: 2.5 mm and 3 mm Allen key and 5.5 Ford key

2. Remove the 4 screws (15) and (16) used to anchor the motor.
1. Loosen the screws (15) and (16) used to anchor the motor.
2. Remove the screw (14) and the part that holds the belt in place.
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Service manual
1. Cut 7 teeth from the belt. When making the cut, ensure that the beginning and end of the steel wire coincide
with the piece being discarded.
2. Check that the vanes and teeth of the belt coincide, to ensure that no teeth have been skipped.
3. Install the new belt and secure it with the part and screw (14).
1. Insert the motor in its housing.
4. Adjust the belt tension to 4 kg, pulling the motor downwards. Start to tighten the screws (15) and then the
screws (16). After tensing the motor, check the belt tension with a tension measuring device. The belt resonance
frequency must be between 130 and 150 Hz.
8.9.7. AC16601 - Stirrer motor/ AC16602 - Stirrer paddle
17
18
19
20
21
Figure 8.31 Assembling the stirrer motor and stirrer paddle
Tools: 1.5 mm and 2.5 mm Allen key

Dismantling the paddle:
1. Loosen the top clamping device (21), grasping the paddle firmly.
2. Carefully lower the whole paddle assembly. The assembly cannot be dismantled.
1. First remove the paddle.
2. Remove the 2 screws (20). Separate the motor from the arm.
3. Remove the 2 screws (19) to separate the motor stirrer adapter.
148
1. Fasten the motor (17) to the adapter (18) of the stirrer with the screws (19).
2. Fasten the assembly to the arm support with the screws (20). Make sure the adapter is placed in the support as
shown in Figure 8.31.
Assembling the paddle:
1. Place the paddle on the motor shaft as far as it will go.
2. Press the clamping device (21) of the paddle until it is firmly secured.
3. Check that the paddle movement is completely axial and uniform.
4. Check and/or adjust the paddle in the different stirrer arm positions. Follow the instructions in chapter 6.6.1.3
of the service manual.
8.10. Fluidic assembly replacement

AC16564 AC16577 AC16578 AC16563
Code Description
AC16563 Fluid board
AC16564 Syringe board
AC16577 Wash station heater
AC16581
AC16578 Wash station
electro valve
AC16579 Pre-dose manifold
AC16582
AC16581 Dispensation
AC16576 pump
AC16582 Suction pump
AC16576 Manifold
AC16580 Water inlet valve
AC16580
AC16579
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Service manual
15
1 4 2 3
Figure 8.32 Fluid and syringe boards
8.10.1. AC16563 - Fluid board / AC16564 - Syringe board

Dismantling:
1. Remove the screw (2) by hand and lower the plate with the electronic boards.
2. Disconnect all the cables of the board to be changed.
3. Remove the electronic board from the separators (4).
Assembly:
1. Change the electronic board.
2. Put it in position.
3. Connect each cable to the board.
4. Raise the board and insert the screw (2) by hand.
8.10.2. AC16577 - Wash station heater

Dismantling:
1. Remove the 2 screws and the clamping device that anchors the heater (5).
2. Disconnect the 2 cables from the heater (6).
3. Unplug all the heater tubes (7). Take care not to deform the tubes when removing them. When removing the tubes
from their housing, do not pull them by their middle parts but by the ends. If they are deformed, replace them.
Assembly:
1. Place the heater with the central hole facing outwards.
2. Connect each heater tube as shown in Figure 8.34.
3. Connect the heater tubes to the electronic board.
4. Insert the clamping device and also the screws (5) that hold the heater in place.
5. Check that it functions correctly with the service programme and whether the temperature must be adjusted.
150
6
Figure 8.33 Dismantling the heater
Figure 8.34 Connecting the heater tubes and electro valves
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Service manual
Figure 8.35 Connecting the electro valve tubes
Figure 8.36 Dismantling the electro valve
8.10.3. AC16578 - Wash station electro valve

Dismantling:
1. Remove the 2 screws anchoring the 5 electro valve assembly (8).
2. Remove all the tubes connecting the electro valves. Take care not to deform the tubes when removing them.
When removing the tubes from their housing, do not pull them by their middle parts but by the ends. If they
are deformed, replace them.
3. Turn the assembly over to access the rear screws.
4. Remove the screws that anchor the electro valve (9).
152
Assembly:
1. Change the electro valve.
2. Put the screws in place (9) to secure the electro valve.
3. Put the 2 screws (8) that anchor the 5 electro valve assembly.
4. Connect all the tubes in each electro valve, as shown in Figure 8.34 and Figure 8.35.
8.10.4. AC16579 - Pre-dose manifold

In addition to the modification of the dispensation pumps, the pre-dose manifold assembly has also been modified.
))
See chapter o see the serial numbers from which the above was implemented.
10
11
Figure 8.37 Changing the pre-dose manifold assembly
Tools: 2.5 Allen key

Dismantling:
1. Remove the clamp used to anchor the tubes and disconnect them.
3. Remove the clamps that anchor the pump tubes to the pre-dose manifold. Turn over the assembly and remove
the screws (11) that anchor each pump.
Assembly:
1. Change the pre-dose manifold.
2. Secure the pumps with the screws and connect the pump tubes to the manifold.
3. Fasten the assembly to the fluidic plate with the screw (10).
4. Connect the tubes, clamps and cables.
5. Check the operation of the manifold and pump electro valves with the service programme.
8.10.5. AC16581 - Dispensation membrane pump / AC16582 - Suction pump

Dismantling:
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Service manual
1. Select the pump to be replaced.

2. Remove the clamp (13) that anchors the tubes and disconnect them.
3. Remove the 2 screws (14) that anchor the pump.
Assembly:
1. Replace the pump.
2. Put the insulation (12) and the new pump in place. Secure it with the 2 screws (14).
3. Connect the tubes, clamps (13) and cables.
12
13
14
Figure 8.38 Change the suction and dispensation pump
8.10.6. AC16580 - Inlet electro valve

Dismantling:
1. Disconnect the tubes. If they are difficult to remove, cut them and replace them with new ones.
Assembly:
1. Replace the electro valve.
2. Screw the electro valve.
3. Connect the tubes and cables.
154
Figure 8.39 Changing the inlet electro valve
8.10.7. AC16576 - Collector
16
Figure 8.40 Changing the collector

Dismantling:
1. Disconnect the collector tubes. Do not remove the collector fittings, as they are sealed. Take care not to deform
the tubes when removing them. When removing the tubes from their housing, do not pull them by their middle
parts but by the ends. If they are deformed, replace them.
Assembly:
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Service manual
1. Replace the collector assembly.

2. Screw the collector in place.
3. Connect all the tubes.
8.11. Sample and reagent rotor replacement

AC16629
AC16632
AC16625 AC16628
AC16813 AC16631
AC16561 AC16623 AC16624 AC16630

AC16562 AC16657
Code Description Code Description

AC16561 Sample rotor board AC16626 Fridge fan
AC16562 Reagent rotor board AC16627 Fridge peltiers
AC16623 Rotor motor AC17229 Plastic separators for peltier
AC16624 Rotor belt AC16630 Reagent rotor cover sensor
AC16632 Reagent rotor centring device AC16657 Sample rotor cover sensor
AC16813 Sample rotor centring device AC16631 Temperature sensor
AC16625 Barcode reader
AC16628 Barcode reader window and heater
AC16629 Barcode reader window
8.11.1. AC16538 - Sample rotor board / AC16562 - Reagent rotor board

The boards of each rotor (sample and reagent) are different, but the installation and dismantling procedure is the
same for both.
Dismantling:
1. Disconnect all the cables connected to the board.
2. Open the 4 clamps to release the board.
Assembly:
156
1. Position and insert the new board inside the 4 clamps.
2. Connect the cables to the board, following the instructions in chapter 4.8.
8.11.2. AC16628 - Barcode reader window and heater
Figure 8.41 Dismantling the barcode reader window
Tools: 2 Ford key

Dismantling:
1. Remove the 4 nuts (1).
2. Disconnect the heater cable.
Assembly:
1. Place the new window in its position with the heater.
2. Secure the 4 nuts and their washers that hold the window in place.
3. Connect the heater cable.
8.11.3. AC16625 - Barcode reader

Dismantling:
1. Remove the screw (2). See Figure 8.41.
2. Cut the clamps holding the cable and disconnect the reader from the electronic board.
There are two validated barcode reader models. The fastening parts for installing both models have also been modified.
))
See chapter AII to see the serial numbers from which the modification of the fastening parts was implemented.
Assembly:
1. Insert the screw holding the reader to the support part.
2. Insert the cable and install the clamping devices to guide the cable through the support.
))
See chapter 3.3 to see how the 2 barcode reader models are installed and adjusted.
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8.11.4. AC16630 - Reagent rotor cover sensor/ AC16657 - Sample rotor cover sensor
Figure 8.42 Assembling the cover sensor
Tools: Loctite 454

Dismantling:
1. Press through the hole with a screwdriver (3) until the sensor (4) is detached.
2. Eliminate all traces of glue that remain in the hole.
3. Disconnect the cable from the electronic board.
Dismantling:
1. Put a little loctite 454 on the side of the sensor. Insert the sensor in the hole and press it until it reaches the end.
2. Connect the cable to the electronic board.
8.11.5. AC16631 - Temperature sensor
6
7
Figure 8.43 Dismantling the temperature sensor
158
Tools: screwdriver, 7 Ford key.
Dismantling:
1. Using a screwdriver, unscrew the temperature sensor through the internal part of the refrigerator (5).
2. Remove the thermal insulation (6). Pull it outwards with the help of some pliers.
3. Unscrew the temperature sensor (7) with the Ford key.
Assembly:
1. Change the sensor (7). Put some thermal silicone in the thread. Screw it into the sensor hole. Tighten with the
Ford key.
2. Insert the insulation (6) as far as the end.
3. Connect the sensor cable.
8.11.6. AC16626 - Refrigerator fan
9
10
Figure 8.44 Dismantling the refrigerator fan before and after the modification
Tools: 3 mm Allen Key

Dismantling:
))
See chapter AII to see the serial numbers from which the modification in the fan aerial connectors was implemented.
1. In analysers with serial numbers that are prior to the modification, disconnect the fan cable from the electronic
board and release the cable from the different clamps that hold it in place.
2. In analysers with serial numbers that are after the modification, disconnect the aerial connector (10) from the fan.
3. Remove the 4 screws (8) or (9) used to anchor the fan.
Assembly:
1. Insert the 4 screws (8) or (9) in the fan.
2. In analysers prior to the modification, insert the nuts and washers in the screws as far as they will go.
3. Install the fan as indicated by the arrows and tighten the screws. Bear in mind that the fan air flow must blow
towards the radiator.
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Service manual
4. Tighten the screws firmly.

5. Connect the fan to the aerial connector or to the electronic board.
8.11.7. AC16627 - Fridge Peltier
11
12
13
14
15
16
17
18
19
Figure 8.45 Dismantling the Peltier assembly
2 2
1 1
Figure 8.46 Positioning and inserting the peltiers
))
See chapter AII to see the serial numbers from which the modification in the peltier assembly was implemented.
Tools: 2.5 Allen Keys
Dismantling:
The peltiers are joined together two by two. When replacing one peltier, both units must be replaced.
1. Loosen the screws (12) to dismantle the fan (11).
2. Loosen the screws (14) to dismantle the radiator (13).
3. If the analyser serial number is prior to the peltier assembly modification, separate each element, peltier (17)
and metal block (19) and eliminate all traces of thermal silicone.
4. If the analyser serial number is after the peltier assembly modification, cut the polyurethane seal slightly (15)
to release the peltier cables and remove the peltier.
160
5. Check the state of the polyurethane seal (15) and the thermal graphite interface (18). If they are damaged, pull
them out. Eliminate all traces of glue from the seal and interface and if necessary, use fine sandpaper.
Assembly:
1. If the analyser serial number is prior to the peltier assembly modification, also change the peltier separators
(spare code AC17229).
2. Position the thermal graphite interface (18), peltier (17) and a second thermal interface (16), duly aligned, as
shown in Figure 8.45.
3. N.B.: Clean the metal block surface (19) with alcohol, to remove all traces of thermal silicone, and also clean
the peltier surfaces (17).
4. The thermal graphite interface (18) is self-adhesive. Remove the protective paper and carefully attach it to the
thermal block.
5. Place the peltier (17) on the thermal interface, making sure the faces are clean.
6. Attach the polyurethane seal (15), with the peltier in the centre and make sure the peltier is level with the seal.
7. Attach the second thermal interface to the peltier.
8. Put the radiator and fan assembly in position and secure the assembly with the screws (14).
9. Connect the fan and peltier to the electronic board.
10. Check that the fridge functions correctly with the service programme.
8.11.8. AC16632 - Reagent rotor centring device / AC16813 - Sample rotor centring device
Tools: 2.5 and 3 Allen Keys
Dismantling:
1. Dismantle the motor and belt, following the instructions in chapter 8.11.9.
2. Dismantle the motor start-up sensor.
3. Remove all the clamps used to anchor the cables to the base.
4. Disconnect all the cables connected to the electronic board.
5. Remove the 4 screws (20) that anchor the centring device to the fridge body or the sample rotor. See Figure 8.47.
6. Remove the 2 insulation parts (21) and remove the 2 screws (22) used to anchor the barcode reader square
support bracket.
Assembly:
1. Change the rotor centring assembly.
2. Put the barcode reader support in place with the 2 screws (22).
3. Put the 2 insulation parts (21) and the fridge body in place and secure with the 4 screws (20). Make sure the
optical window of the vessel is facing the barcode reader.
4. Connect all the cables to the electronic board. Chapter 4.8 explains how to connect the cables to the connectors.
5. Assemble the start-up sensor.
6. Assemble the belt and the motor following the instructions in chapter 8.11.9.
7. Check the assembly operation with the service programme (motor, fridge, cover sensor, temperature sensor,
barcode reader).
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Service manual
20
Figure 8.47 Dismantling the fridge body from the rotor centring device
22
21
Figure 8.48 Dismantling the insulation and reader square support bracket
8.11.9. AC16623 - Rotor motor / AC17239 - Rotor motor / AC16624 - Belt

A modification has been made to the rotor motor transmission system. This modification affects the reagent rotor
and the sample rotor.
Use spare code AC16623 for analysers with serial numbers before the one indicated in chapter AII and use code
AC17239 for analysers with more recent serial numbers.
))
See chapter AII to see the serial numbers from which the modification in the transmission assembly was implemented.
Tools: 3 Allen key and Ford key.
Dismantling the AC16623 motor:
1. Disconnect the motor cable. See Figure 8.49.
2. Remove the 4 screws (24) and (25) used to anchor the motor.
162
3. Remove the motor (26).
1. Remove the screws (24) and (25) fastening it to the motor.
1. Insert a new belt into the pulley.
Assembling the AC16623 motor:
1. Insert the motor (26) in its housing.
4. Adjust the pulley tension by pulling the motor outwards. The pulley tension is adjusted to 5.5 kg. Tighten the
screws starting with screw (25) and then tighten screw (24).
23
24
25
26
Figure 8.49 Dismantling the AC16623 motor and/or belt
Dismantling the AC17239 motor:

1. Disconnect the motor cable. See Figure 8.50 and Figure 8.51.
2. Unscrew the 4 nuts (31) anchoring the board (28) to the motor (27).
3. Remove the 4 screws (30) anchoring the motor to the board.
1. Loosen the nuts (31) to slacken the board (28) that secures the motor.
1. Insert a new belt into the pulley.
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Service manual
Assembling the AC16623 motor:

1. Assemble the motor (27) with the 4 screws (30) on the board (28).
2. Insert the board (28) installed with the motor on the 4 screws (29). Position the nuts (31) and washers without
tightening them.
3. Install the belt (23) on the motor pulley.
4. Adjust the pulley tension by pulling the motor outwards. The pulley tension is adjusted to 5.5 kg. Tighten the
nuts (31) crosswise.
27
28
23
Figure 8.50 Identification of new transmission design elements
29
30
31
Figure 8.51 Dismantling the AC17239 motor
164
8.12. Ceramic pump spares
AC16566 AC16566
AC16594
AC17255 AC17254
AC16593
AC16591
AC16590
AC16565 AC16592
AC16592
AC16565

AC17255 Reagent pump manifold
AC17254 Sample pump manifold
AC16590 Reagent piston stop
AC16591 Sample piston stop
AC16592 Pump motor + spindle
AC16593 Sample ceramic piston
AC16594 Reagent ceramic piston
AC16565 Start-up sensor board
AC16566 Led manifold board
During the pump spares handling process, make sure you do not touch the manifold window with your fingers.
8.12.1. AC16565 - Start-up sensor board / AC16566 - Manifold led board

Dismantling the LED board:
1. Disconnect the two cables from the LED board.
2. Remove the 3 clips (1) and cover (2) of the pump assembly.
3. Remove the LED board (3).
Assembling the LED board:
1. Replace the LED board (3). Put the board in its correct position and insert it in the slot.
2. Put the cover (2) and the 3 clips (1) that secure the cover on the pump.
3. Check that the cover is correctly positioned and adjusted with respect to the manifold window (4).
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Service manual
4. Connect the 2 cables of the LED board. Turn on the analyser to check the LED operation.
Dismantling the start-up sensor board:
1. Disconnect the cable from the start-up sensor board.
2. Remove the 2 screws (5) used to anchor the board.
3. Remove the start-up sensor board (6).
Assembling the start-up sensor board:
1. Replace the start-up sensor board (6).
2. Insert the 2 screws (5) that anchor the board. Align the start-up sensor board when you tighten the screws.
3. Connect the start-up sensor cable. Check the operation with the service programme.
4 3
5
6
Figure 8.52 Dismantling the LED and start-up sensor board
8.12.2. AC17255 - Reagent manifold / AC17254 - Sample manifold

The assembly and dismantling process is the same for the reagent manifold and for the sample manifold.
Dismantling:
1. Remove the cover and the led board. See chapter 8.12.1.
2. Remove the 4 screws (7) and detach the manifold (8) from the rest of the pump. Carefully pull the manifold
to remove the piston from the stop supplied with the manifold.
3. Turn the manifold over and remove the 4 screws (13) to separate the chamber support parts (9), O-ring (10),
bubble suppressor (11) and stop adapter and stop (12).
166
9
7
10
11
12
13
Figure 8.53 Dismantling the manifold
Assembly:
1. Replace the manifold.
2. Correctly position each part (9) as shown in Figure 8.54.
3. Turn the manifold, holding the parts (14).
4. Check that the O-ring (14) is clean. Put it in its housing. Check that it has been correctly inserted and that it
does not leave the guides (15). See Figure 8.55.
5. Check that the bubble suppressor (16) has no burrs or dirt. Place it on top of the O-ring. Once assembled,
make sure the O-ring is not visible. See Figure 8.55.
6. Correctly position the stop adapter and stop (17). The flat side of the adapter must coincide with the manifold
window (18). See Figure 8.56.
7. Tighten the 4 screws (19) with a torque of 3.0 Nm, crosswise. See Figure 8.56.
8. Lubricate the piston and stop with WS (Washing Solution).
9. Correctly position the manifold with the pump. The manifold window (21) must coincide with the pump
guide screw (22). See Figure 8.57.
10. Insert the piston in the stop.
11. Press the manifold against the pump, put the 4 screws in places (20) and tighten crosswise with a torque of
6.0 Nm.
12. Assemble the pump on the instrument. Connect the motor cable and start-up sensor cable. Connect the tubes.
13. Check the operation of the pump with the service programme.
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Service manual
Figure 8.54 Assembling the chamber anchoring parts
14
16
15
Figure 8.55 Assembling the manifold
168
17
19
18
Figure 8.56 Assembling the manifold
20
21
22
Figure 8.57 Assembling the manifold on the pump
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Service manual
8.12.3. AC16590 - Reagent piston stop / AC16591 - Sample piston stop

Dismantling:
1. Follow the steps for dismantling set out in chapter 8.12.2.
2. Separate the stop from the stop adapter.
Assembly:
1. Follow the steps for assembling set out in chapter 8.12.2.
2. Before executing point 7, replace the stop (23) with a new one. Wet the stop adapter and the stop with WS
(Washing Solution) to make it easier to insert the stop in its housing. Use a flat element to press the stop.
3. Check that the stop is properly levelled with its support.
4. Follow the steps for assembling set out in chapter 8.12.2.
23
Figure 8.58 Installing the stop
8.12.4. AC16593 - Ceramic sample piston /AC16594 - Ceramic reagent piston /AC16592 -
Pump motor + spindle
Tools: 2 Allen key and 2.5 Ford key, ELESA NT1 grease
Dismantling:
1. Follow the steps for dismantling set out in chapter 8.12.2.
2. Dismantle the start-up sensor.
3. Remove the 4 screws and nuts anchoring the pump body (24) to the motor.
4. Remove the pump body (24).
5. Unscrew the piston with the support by turning it.
170
Assembly:
1. Replace the faulty part (motor + spindle) or ceramic piston.
2. Grease the piston support guide. Apply sufficient grease to the support hole through which the spindle enters.
3. Screw on the piston support with the spindle. Remove all excess grease with a cloth.
4. Insert the pump chamber. Align the groove of the support (26) with the body anti-rotation system (25).
5. Put the screws and nuts to secure the body to the motor.
6. Put the start-up sensor.
7. Follow the steps for assembling the manifold set out in chapter 8.12.2.
24
25
26
27
Figure 8.59 Greasing the spindle and assembling the pump body
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Service manual
8.13. Reaction rotor spares
AC16617 AC17240
AC16597
AC17216
AC16620
AC16595
AC16596
AC16575
AC16615
AC16565 AC16565
AC16621
AC16619
AC16616
AC16622
AC16618
AC16614
AC16560
AC16559
172
AC16617 Rotor centring device
AC17216 Reagent rotor cover sensor
AC16620 Reaction rotor temperature sensor
AC17240 Wash station photosensor
AC16597 Wash station tip spring
AC16595 Wash station suction tip
AC16596 Wash station suction tip + dryer
AC16565 Photobarrier board
AC16619 Wash station vertical belt
AC16618 Wash station elevation motor
AC16575 Reaction rotor support column
AC16615 Reaction rotor belt
AC16621 Rotor thermostatting fan assembly
AC16616 Reaction rotor motor
AC16622 Reaction rotor peltier assembly
AC16614 Optical bench assembly without filters
AC16560 Photometry board
AC16559 Photometry control board
FI15128 340 Filter
FI15129 405 Filter
FI15130 505 Filter
FI15131 535 Filter
FI15132 560 Filter
FI15133 600 Filter
FI15134 635 Filter
FI15135 670 Filter
AC17226 Reaction rotor assembly
8.13.1. AC16617 - Rotor centring device

Tools: 1.5 Allen Key and loctite 243
Dismantling:
1. Unscrew the rod of the (1) rotor centring device. See Figure 8.60.
2. Pull the rotor centring device upwards to dismantle it.
Assembly:
1. Replace the rod (1) with a new one. Do not reuse it.
2. Put a little Loctite 243 (screw fixers) on the rod thread (1).
3. Place the rod in its position in the rotor centring device (2).
4. Place the rotor centring device (2) facing the rod side with the shaft groove (3).
5. Tighten the rod (1).
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Service manual
Figure 8.60 Assembling and dismantling the rotor centring device
8.13.2. AC17216 - Cover sensor

Tools: Loctite 454
Dismantling:
1. Press through the hole with a screwdriver (4) until the sensor (5) is detached.
2. Eliminate all traces of glue that remain in the hole.
Assembly:
1. Put a little loctite 454 on the side of the sensor. Insert the sensor in the hole and press it until it reaches the end.
2. Connect the cable to the electronic board.
3. Check the operation with the service programme.
Figure 8.61 Replacing the cover sensor
174
8.13.3. AC16620 - Temperature sensor
Tools: 2.5 Allen key, thermal silicone
Dismantling:
1. Remove the screw (9).
2. Remove the sensor (7) and its support (8) plate.
Assembly:
1. Replace the sensor (7) with a new one.
2. Clean all remains of silicone from the sensor (6) hole.
3. Apply a little thermal silicone to the hole (6).
4. Install the temperature (7) sensor and plate (8) and place the screw (9) in its housing.
5. Check and adjust the rotor temperature with the service programme.
Figure 8.62 Changing the temperature sensor
8.13.4. AC17240 - Wash station collision sensor

Dismantling:
1. Remove the screws (10) used to anchor the collision photosensor.
2. Disconnect the aerial connector (12) from the other end of the sensor.
3. Remove the pins (13) from the connector.
4. Pull the cabling out of the guide tube (11).
Assembly:
1. Change the collision photosensor.
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Service manual
2. Remove the pins from the connector (13).

3. Insert the sensor cabling pins through the top part of the guide tube (11) of the wash station.
4. When the cabling emerges through the lower part, pull the cable, leaving a clearance of 3 cm at the top.
5. Insert the screws (10) to anchor the collision photosensor.
6. Place the pins in the connector in the order shown in Figure 8.63.
7. Connect the aerial connector (12).
10
11
12
13
Figure 8.63 Changing the wash station collision sensor
8.13.5. AC16597 - Wash station tip spring

Dismantling the dryer spring:
2. Remove the safety washer and the flat washer.
176
3. Remove the faulty spring (15).
Assembling the dryer spring:
1. Replace the spring with a new one (15).
2. Insert the flat washer, safety washer and screw (14).
3. Check that the dryer rises and lowers gently, with no jolting.
Dismantling the clamp foot spring:
2. Raise the guide screw (16) high enough to be able to remove the faulty spring (17).
Assembling the clamp foot spring:
1. Replace the spring (17) with a new one.
2. Install the guide screw (16) as far as the clamp foot (18).
3. Tighten the screw (19).
4. Check that the clamp foot rises and lowers gently, with no jolting.
14
16
15
17
18
19
Figure 8.64 Dismantling the wash station springs
8.13.6. AC16595 - Wash station suction tip / AC16596 - Suction tip + wash station dryer
Tools: 1.5 Allen key, loctite 243, loctite 495
Dismantling the suction and dispensation tube:
1. Disconnect the 2 silicone tubes of the tube that is to be replaced.
2. Unscrew the rod (21).
3. Remove the suction (20) and dispensation tube.
Assembling the tube:
1. Replace the tube with a new one (20).
2. Put a drop of loctite 243 on the rod thread.
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Service manual
3. Ensure the tube (20) is facing the flat side and pressing the rod (21).
4. Level the tube at the same depth (22) as the adjacent ones.
Dismantling the dryer:
1. Pull the dryer part (25) until it becomes detached.
2. If you also have to change the tubes, unscrew the rods (24) to release the tubes (23).
Assembling the dryer:
1. Replace the tube (23) with a new one.
2. Level both tubes (23) at the same depth.
3. Put a drop of loctite 243 on the rods (24).
4. Tighten the rods (24).
5. Put some loctite 495 around the tube (23) and dryer (25) to seal the joint.
6. Adjust the positioning of the wash station using the service programme.
23
20
21 24
25
22
Figure 8.65 Changing the dispensation tube and dryer
8.13.7. AC16565 - Photobarrier board

Dismantling:
1. Disconnect the cable from the photobarrier board.
Assembly:
1. Replace the board with a new one (26).
2. Align the board so that the plate does not collide with the photosensor.
178
3. Tighten the 2 screws (27) and connect the cable to the photobarrier.
26
27
Figure 8.66 Changing the wash station photobarrier
8.13.8. AC16618 - Wash station elevation motor / AC16619 - Wash station vertical belt
31
28
29
30

Figure 8.67 Changing the wash station motor
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Service manual

2. Unscrew the 4 screws (28) and (30) and their safety washers.
1. Loosen the screws (28) and (30) securing the motor.
3. Remove the belt.
1. Replace the motor (29).
2. Put the screws (28) and (30), washers and nuts in position, without tightening them.
3. Insert the belt through the pulley.
4. Tauten the belt to 4 kg. Tighten the screws starting with screws (28) and then the (30).
5. Check the belt tension with a frequency meter. The frequency must be between 150 Hz and 170 Hz.
1. Replace the belt.
2. Check the belt teeth and match the vanes with the teeth to make sure no teeth are skipped.
3. Insert the belt through the pulley and counter pulley and join both ends of the belt to the closing device. Insert
and tighten the screw (31).
4. Tauten the belt and the motor as indicated in points 4 and 5 of the motor assembly section.
8.13.9. AC16575 - Reaction rotor support column
32
33
34
Figure 8.68 Changing the support column
180
Dismantling:
2. Remove the column (33).
Assembly:
1. Replace the column.
2. Check the column direction. It has a positioning device (34).
3. Insert the washer and screw (32) and tighten it.
8.13.10. AC16615 - Reaction rotor belt / AC16616 - Reaction rotor motor
35
36
37
38
Figure 8.69 Changing the rotor motor and belt

2. Unscrew the 4 screws (36) and (38) and their safety washers.
1. Loosen the screws (36) and (38) securing the motor.
1. Replace the motor (37).
2. Put the screws (36) and (38), washers and nuts in position, without tightening them.
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Service manual
3. Insert the belt through the pulley.

4. Tauten the belt to 5 kg. Tighten the screws starting with screws (36) and then the (38).
1. Replace the belt.
2. Check the belt teeth and match the vanes with the teeth to make sure no teeth are skipped.
3. Insert the belt through the pulley and the counter pulley.
4. Tauten the belt and the motor as indicated in points 4 and 5 of the motor assembly section.
8.13.11. AC16621 - Rotor thermostatting fan assembly

Dismantling:
1. Unscrew the 4 screws (39) and their safety washers.
2. Disconnect the fan from the electronic board.
3. Remove the fan (40).
Assembly:
1. Replace the faulty fan.
2. Insert the 4 screws (39) and their safety washers in the support at the base.
3. Position the fan in the correct direction. The air flow (41) must go towards the radiator.
4. Tighten the 4 screws crosswise.
5. Connect the fan to the electronic board and check it is operating correctly.
39
40
41
Figure 8.70 Changing the fans
8.13.12. AC16622 - Reaction rotor peltier assembly

182
Dismantling:
1. The peltiers are joined together two by two. Peltiers (42) and (43) have the same cables. Peltiers (44) and (45)
have different cables. When replacing the peltiers, you will have to replace the two-peltier assembly.
2. Remove both fans from the peltier assembly to be replaced, as indicated in chapter 8.13.11.
3. Remove both the radiators. Remove the 4 screws (48). Also remove the safety washers and plastic washers to
isolate the screws.
4. Remove the two-peltier assembly.
Eliminate all remains of silicone from the base and the radiator. Apply a little alcohol to remove the traces of sili-
cone. Assembly:
1. Replace the two-peltier assembly.
2. Position the 2 peltiers correctly. Check the positons of the red (47) and black (46) cables.
3. Apply a thin layer of silicone to both sides of the peltier. Do not apply too much silicone to ensure it does not
emerge from the sides and cause a thermal bridge.
4. Position the radiator (49) with the 4 screws (48) and tighten them crosswise.
5. Position the fans as shown in chapter 8.13.11.
6. Check and adjust the temperature with the service programme.
42
48
49
43
44
46
47
45
Figure 8.71 Changing the peltiers
8.13.13. AC16559 - Photometric control board - CIIM0050

Dismantling:
1. Disconnect all the cables connected to the photometric control board.
2. Remove the board (50) from the supporting parts.
Assembly:
1. Replace the board (50).
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Service manual
2. Make sure the board is facing the right direction and put it in position.
3. Connect each cable in its correct position. Chapter 4.6 explains how to connect each of the cables.
50
Figure 8.72 Changing the photometric control board
8.13.14. AC16560 - Photometric board - CIIM0051

Dismantling:
1. Disconnect all the cables connected to the photometric control board - CIIM00050. Release the cables from
the parts that
2. Remove the 4 screws (51) attaching the CIIM0051 photometric board to the rotor. One of the screws is con-
nected to the ground wire (52).
3. Separate the assembly formed by the 2 boards (photometric and photometric control) from the rest of the rotor.
4. Disconnect the flat strip connecting the photometric board to the photometric control board.
5. Remove the 2 screws (54) and their washers and safety washers attaching the photometric board to the support
part.
6. Remove the 4 screws (55) attaching the optical bench to the board.
7. Remove the clamp (57).
8. Take care with the photodiode protectors (56) when separating the optical bench from the electronic board.
Assembly:
1. Replace the board (53).
2. Place the protectors on each photodiode correctly (56).
184
51
52
53
Figure 8.73
54
Figure 8.74
185
Service manual
55
56
57
Figure 8.75
3. Insert the screws (55) that attach the optical bench to the electronic board. Tighten them with a torque of 3.8 Nm.
4. Insert the clamp (57).
5. Insert the 2 screws (54) attaching the photometric board to the support. Do not tighten them.
6. Connect the flat strip connecting the photometric board to the photometric control board.
7. Tighten the 4 screws (51) to joint the electronic board assembly to the rotor. Then connect the ground wire
(52) of the reaction rotor assembly.
8. Connect all the cables to the photometric control board. Chapter 4.6 explains how to connect each of the cables.
Assembling the reaction rotor assembly to the instrument:
9. Place the rotor assembly in its position on the instrument.
10. Loosen the screws (61) and (62) that anchor the photometric board.
11. Insert and firmly tighten the screws in the following order: first screw (60), then screws (64) and (65) and finally
screws (58) and (59).
12. Then tighten screws (61) and (62) using a torque of 3.8 Nm.
13. Put screw (63) in position and tighten it to connect the ground wire to the instrument base.
186
58
59
60
Figure 8.76
61
62
Figure 8.77
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Service manual
64
65
63
Figure 8.78
8.13.15. AC16614 - Optical bench assembly without filters / Changing the filters
Dismantling the optical bench:
1. Follow the steps for dismantling set out in chapter 8.13.14 to separate the optical bench.
2. Remove each filter from the optical bench.
Assembling the optical bench:
1. Replace the optical bench.
2. Place each filter in the position shown in Figure 8.79.
3. Follow the assembly process described in chapter 8.13.14.
Dismantling the filters:
1. Follow the steps for dismantling set out in chapter 8.13.14 to separate the optical bench.
2. Remove the damaged filter. Locate the position as shown in Figure 8.79.
Assembling the optical bench:
1. Replace the filter with a new one.
2. Follow the assembly process described in chapter 8.13.14.
188
405 635 505 535 600 670 560 340
Figure 8.79 Filter positioning order
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Service manual
9. Troubleshooting
9.1. Software and firmware installation process
As a precautionary measure to prevent accidents, it is a good idea to make a backup copy of the programme and
the database.
9.1.1. Backup copy before executing the version update

1. Open the BA400 programme.
2. In the menu, select: Functions/Create a restoration point with the current data
3. Click on save.
4. Close the programme.
5. In My PC enter the folder: c:\Program Files (x86)\BA400\
6. Select the User Sw folder and Copy (CRTL-C)
7. Paste (CRTL-V) in the same folder. A new folder called User Sw – copy will be created
9.1.2. Updating the version

• If the installation programme downloads it from the ftp.
1. Create a folder in the computer called: C/Temp
2. Copy the .zip file downloaded from the ftp into the c:/temp folder. Do not copy it onto the desktop. It is im-
portant to copy it in the c:/temp folder because the installer contains subfolder routes that are very long and
may be copied incorrectly.
3. Make sure that the .zip file is intact by calculating the MD5 checksum.
4. You can perform the MD5 with the HJSplit programme. It is located in the website: http://www.hjsplit.org/
5. Decompress the .zip file in the C:/Temp folder
6. Carry on from step 8.
• If the installation programme is executed from a DVD
7. Open the DVD reading unit and insert the DVD with the new version.
8. Execute the setup.exe programme as the administrator (Select the file, right-click with the mouse and select the
option: execute as administrator).
9. Follow the steps in the installation process.
10. Execute the BA400.exe programme. First of all the programme will execute the database update process. Once
the process had ended a message will appear saying that the analyser firmware version is different and must be
updated.
11. The programme automatically makes a copy of the database of the previous version and stores it in the folder:
c:\Program Files (x86)\BA400\User Sw\RestorePoints\
• Firmware update process
1. Close the user programme.
2. Close and open the analyser switch.
190
3. Open the service programme.
4. The firmware update screen will open automatically.
5. Select the firmware file that corresponds with the software version. Route c:\Program Files (x86)\BA400\Firmware\
6. Press the firmware update option.
7. That process takes a few minutes.
8. Once completed, close the application.
9.1.3. Patch update

Not all software versions have a match that must be installed. When the documentation indicates that there is a
patch, follow the steps for installing the patch:
1. Install the version or update.
2. Install the patch. Execute the vx.x.x_Patch1.exe programme. The x values indicate the version to be installed.
9.1.4. Revert to a previous version

If you have to return to the previous software version for any reason, perform the following steps:
1. Uninstall the BA400 version. Open the Windows uninstaller programme. Route: My PC/Control Panel/Pro-
grammes and characteristics
2. Select BA400
3. Uninstall.
4. Install the previous software version following the steps given at the beginning of this guide.
5. Execute the BA400.exe application
6. When the application is initiated, it retrieves the previous version of the database. A window will automatically
pop up for you to select the previous database file. Select that file and press accept.
9.1.5. Solving problems related to installation

The SQL Server database engine is not correctly installed:
1. Check that there is only one version of the SQL Server database engine and that the version is the correct one.
2. Check that the SQL Server programmes installed are like the ones shown in Figure 9.1.
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Service manual
Figure 9.1 SQL Server programmes installed
3. If the application has been installed from a .zip file, check that it has been decompressed in the c:/Temp folder
and that there was no error in decompressing the file.
9.1.6. Solution
1. Manually uninstall each of the SQL Server database engine entries.
2. Ensure that the file is not corrupted. Confirm the size of the version installation file with your technical service.
3. Follow the steps from section 9.1 of this chapter.
9.1.7. Solving of problems related to the computer configuration

When a pop-up window appears with the error:
“Operation with Visual Style, came to error because now no active styles representation.”
Proceed as follows:
Activate the Windows visual styles:
1. Open the following route: Initial menu/Control panel/system/advanced system configuration
192
Figure 9.2
2. Select Advanced options and configuration. See Figure 9.3

3. Activate the “Use visual styles on windows and buttons” option. See Figure 9.4
4. Accept the changes
Figure 9.3
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Service manual
Figure 9.4
9.1.8. Problems in installing the SQL Server

When an error window appears while installing the SQL server database like the one in Figure 9.5, check the fol-
lowing user privileges for the Windows operating system.
Figure 9.5 Error message in installing the SQL database
194
1. Select the programme: Control panel/Administrative tools/Local security directive
2. Select the option: Account directive/Password directive
3. Check that the following option is deactivated: Password must meet the complexity requirements. See Figure 9.6
Figure 9.6
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Service manual
9.2. ISE module maintenance

This chapter explains the processes for maintaining the ISE module and for solving problems related to it.
9.2.1. Changing the electrodes

When changing the electrodes, follow the instructions given in the manual. Remember to execute a maintenance
cycle before removing the electrodes. This cycle empties the fluid into the fluid channel to ensure that no traces of
the calibration solution remain when the electrodes are removed.
Once removed, dry the electrodes immediately to ensure that no traces of the calibration solution and/or salts are
left on the contacts.
When calibrating the installation process, make sure there is no significant difference (>2 units) between them. If
this should occur, the electrode membranes need more time to reach their optimum operating level. It is not advis-
able to take measurements until this difference disappears. The process can be accelerated by circulating control
serums (make ISE determinations).
9.2.2. Check that the fluid system is operating correctly.

• Check that the reagent pack is not exhausted.
• Ensure the correct positioning of the connector to the reagent pack. Check that the connector is not slightly
raised, thus preventing contact between the connector and the pack.
• Check that the contact zone between the connector and the pack is clean.
• Press the side of the reagent pack with your hand to prevent the obstruction of CAL A or CAL B due to the
waste bag being full.
• Check that the fluid front in the Teflon tubes of CAL A and CAL B is not broken up. If the fluid front is broken,
this means that the pack is almost exhausted or that air is entering the fluid circuit.
• Check to see whether the liquid moves through the tubes after the pumps have stopped. This movement may
indicate that air is entering at some point in the fluid system or through its seal.
• Check that the electrodes are properly positioned and that there are no fluid leaks.
• Check that the O-rings are properly positioned between the electrodes.
• Check that there are O-rings in each of the openings in the reagent pack.
• Check that the lower elbow of the electrodes does not protrude and that it is properly positioned.
• Check that the peristaltic pumps are running correctly.
• Perform the following calibrations: Peristaltic pump, air bubble detector and electrode calibration. Check that
the results are within the margins.
9.2.3. Pass a nylon cord through the opening

If the electrode probe is blocked, clean it with a cloth dampened with distilled water and if it cannot be cleaned,
pass a cord through to unblock the electrode probe.
If the nylon cord is not stiff enough to unblock the probe, use the fine-tipped syringe supplied in the accessory box.
Take care not to damage the electrode membrane walls with the cord. Do not force the cord too much.
If you cannot eliminate the blockage caused by the saline solution in the reference electrode, apply lukewarm water
for about fifteen minutes to dissolve the salts and try again with the cord.
If the blockage is due to simple remnants, perform the cleaning operation once the blockage has been removed.
196
If the blockage is recurrent, use the cleaning solution more often.
After a blockage, it is important to check that the calibration results remain stable. Sample remnants could be left
in the membrane that could affect the result.
9.2.4. Clean the probes and metal contacts of the electrodes and the ISE module with a cotton
bud
The electrodes have saline solutions with high concentrations inside them. Sometimes, the remains of the calibra-
tion solution dry and leave invisible salt traces on the contacts or the probe, which may cause short-circuiting or
interfere with the readings.
Turn off the analyser and remove the electrodes, clean the metal contacts and membrane probe with a cloth damp-
ened with distilled water in each electrode. Ensure that the area is completely dry before reinstalling the electrodes.
9.2.5. Wipe the cup to remove all sample traces

Once a week, clean the cup with a cotton bud soaked in distilled water, to prevent the blocking of the cup input
opening in the ISE module.
9.2.6. Calibration acceptance margins

The acceptance margins for calibrating the electrodes and for calibrating the peristaltic pump are set out below:
Electrode Calibration margin

Li+ 47-64 mV/dec
Na+ 52-64 mV/dec
K+ 52-64 mV/dec
Cl- 40-55 mV/dec
The calibration results may be gradually reduced over time, as the electrodes age. A sudden change is not in keeping
with the expected behaviour. If this should occur, check the status of the module (tubes, electrodes, reagent pack)
before continuing the measurements.
Peristaltic pump Calibration margin

Waste pump
Cal A pump 1500 - 3000
Cal B pump 1500 - 3000
Air bubble detector Calibration margin

BBC A Number of steps to move 75 uL of CAL A
BBC L Number of steps to detect the air
BBC M Delta between the value of A and L. It must be
over 90
9.2.7. Noise and drift errors

Low gradients are generally the result of a loss of sensitivity in an electrode over time, but it could also be due to
other causes. Noise errors indicate instability in the values of a solution given during an analysis. Drift indicates that
the analyser is not observing stable values between the measurements of the calibration solutions.
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Service manual
To solve the problem, first flush the machine with the daily solution a couple of times to remove all remnants of
accumulated proteins in the fluidic channel. If the problem persists, check that the routine maintenance has been
performed, such as replacing the reference electrode, the O-ring, etc. If it still does not work, replace the faulty
electrode and confirm that the problem has been solved. If not, the cause could be contamination by salts.
In all ion-specific electrodes, there is a possibility of the dried salt or damp providing an electrical current leak
route which could lead to errors, including “drift”, “noise” or incorrect gradient values. The electric signal coming
from the ISE electrodes is very small and any interference with these signals could give rise to errors. While using
the analyser, tiny quantities of the calibration solutions may filter through, leaving trails of damp or traces of salt
that may not be visible at first sight. Damp or traces of salt conduct electricity and may lead to short circuits in the
electrodes that interfere with the measuring.
To eliminate these short circuits that cause errors in the signal, remove the trails of damp or traces of salt. The best
way to eliminate them is to turn off the analyser ISE module, take all the electrodes out of the analyser and clean
each contact with a damp paper towel and then let them dry. The next step is to remove all traces of damp or salt
from the area where the electrodes make contact with the ISE module with a damp paper towel. Dry the contacts
with a dry paper towel. When you are sure that all elements are dry, reinstall the electrodes and try again.
Also check the electrode contacts. Make sure the contacts are clean. If they are dirty or corroded, clean them gently
with a pencil eraser (be careful not to remove the thin gold layer). The contacts in the module are tightened with a
spring. Check that the springs are functioning correctly and that the contacts enter and emerge correctly.
Another potential source of "noise" errors is related to the fluidic channel. When the peristaltic pump stops, the
fluid also stops. The “noise” is generated when the solution continues to move while the sample or calibrator is
being measured. The error “noise in Cal A” is generated when the ISE modules reads, while calibrator A is present.
What really occurs is that the ISE module takes six readings in rapid succession. Then the ISE module calculates
the mean of all six readings. If one of the readings has a difference of 0.7mV above or below the mean, then it will
indicate a “noise” error.
This may occur due to a small problem in the flow and Calibrator A moves when the reading is taken. Check that
all the electrodes are correctly placed and that the O-rings are present. A quick test to check this is to dispense Cal
A into the Sample cup and observe whether the solution remains inside the cup. If it empties slowly, there is a small
leak. Check that the pump tubes have been replaced as part of the routine maintenance programme.
The noise may also be generated if the reference electrode has an age of more than six months. Make sure that
maintenance is performed when necessary.
Obviously, if an electrode is constantly generating noise errors, it must be replaced.
“Drift in Cal A” is generated after a sample analysis. After each sample analysis, it is placed in front of the calibrator
A electrodes and a reading is taken. Then the result obtained is compared against the previous reading of calibra-
tor A. If the difference exceeds the limit established by the manufacturer, a “drift” error is obtained. To solve the
problem, follow the steps in the procedure indicated above for "noise" errors.
But in both cases, try to execute a cleaning and recalibration cycle as the first step.
If the problems persist, they are likely due to external factors, such as a faulty or intermittent ground connection,
magnetic field perturbations or the effects of other instruments such as the electric motors of the refrigerators or
centrifuges.
198
AI. List of accessories and spares
In the event of any of the analyser components being damaged or if any fungible goods are required, always use
original BioSystems material.
The following table shows a list of the components that might be needed. To purchase them contact your habitual
distributor and ask for each element with its respective code.
Accessories list
Code Representation Description
AC16359 DVD with User Program
AC11485 Reaction Rotor (10)
AC10770 Sample wells (1 000)
AC16434 500 mL bottle of concentrated washing solution
AC17201 Bottle of acid washing solution
AC16360 Open adapter for primary tubes (90)
AC16361 Closed adapter for sample wells (45)
199
Service manual
Accessories list
AC16362 60 mL reagent bottles (20)
AC16363 20 mL reagent bottles (20)
AC16364 60 mL brown reagent bottles (20)
AC16365 20 mL brown reagent bottles (20)
AC16366 Thick and thin connection tube for distilled water bottle (3
m)
AC16367 Connection tube for waste (3 m)
AC16368 Washing solution bottle with cap
200
Accessories list
AC16369 High contamination bottle with cap
AC16748 Washing solution cap
AC16749 High concentration waste cap
AC16370 Reagent rotor
AC16371 Sample rotor
AC11486 Reaction rotor set screw
CA10455 European mains cable
CA10456 American mains cable
FI14226 USB cable for connection to PC
AC16747 10 A fuse (2)
201
Service manual
Accessories list
AC17097 Entry filter set for purified water (150 µm)
AC16791 Entry filter water set (5 µm)
AC16792 Spare water filter (5 µm)
List of ISE module accessories (optional)

5420 Standard pack
5625 Tube assembly
5201 Na+ electrode
5202 K+ electrode
5207 Cl- electrode
5205 Li+ electrode
5206 Spacer electrode
202
List of ISE module accessories (optional)
5204 Reference electrode
5421 ISE module washing solution KIT
5412 ISE 125 mL urine dilution module
AC16752 ISE cleaning kit
AC17096 Cotton buds
203
Service manual
List of spares
AC16533 DVD Service programme and Service Manual
AC16534 Reaction rotor screw
AC16535 Reaction rotor cover
AC16536 Sample rotor cover
AC16537 Reagent rotor cover
AC17095 General cover
AC16538 Arm cover
AC16539 Top cover of wash station
AC16540 Lower cover of wash station
204
List of spares
AC16541 Lower cover of arm
AC16542 Lower cover of stirrers
AC16543 ISE module cover
AC16544 Door assembly
AC16545 Lateral cover assembly
AC16546 Rear cover handles
AC16549 Hinges of general cover
AC16550 Front led frame
AC16551 Legs
205
Service manual
List of spares
AC16552 Wheels
AC16553 Filter and network connector
AC16554 Switch assembly
AC16555 CPU board
AC16556 Distribution board
AC16557 Arm board
AC16558 Tip board
AC16559 Photometry Control Board
AC16560 Photometry board
AC16561 Sample rotor board
206
List of spares
AC16562 Reagent rotor board
AC16563 Fluid board
AC16564 Syringe board
AC16565 Photobarrier board
AC16634 AC input board
AC16566 Led Manifold Board
AC16567 320 W power supply
AC16569 35 W power supply
AC16570 Load cell
207
Service manual
List of spares
AC16571 General fan (2 wires)
AC16572 Distilled water bottle with cap and buoys
AC16573 Low contamination waste bottle with cap and

buoys
AC16574 Tip wash station
AC16575 Reaction rotor support column
AC16576 Manifold assembly
AC16577 Wash station heater
208
List of spares
AC16578 3-way electro valve
AC16579 Pre-dose Manifold
AC16580 Water inlet valve
AC16581 Dispensing pumps
AC16582 Suction pumps
AC16583 External water inlet adapter
AC16584 Water tank inlet adapter
AC16585 Wash station pump stop + O-ring
AC16586 Wash station pump motor
AC16587 Top chamber
209
Service manual
List of spares
AC16588 Wash station pump motor assembly
AC16817 Wash station clamping device
AC16816 Pistons and support assembly
AC16589 Manifold
AC16590 Reagent piston stop
AC16591 Sample piston stop
AC16592 Pump motor+spindle
AC16593 Sample ceramic piston
AC16594 Reagent ceramic piston
AC16595 Wash station suction tip
210
List of spares
AC16596 Wash station suction tip+dryer
AC16597 Wash station tip spring (4)
AC16598 Vertical movement belt

AC16599 Polar movement belt
AC16600 Arm motor
AC16601 Continuous current stirrer motor
AC16602 Stirrer blade
AC16805 Stirrer motor cable (2)
AC16603 Sample tip
AC16604 Reagent tip
AC16605 Tip spring
FI15128 340 Filter
FI15129 405 Filter
FI15130 505 Filter
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Service manual
List of spares
FI15131 535 Filter
FI15132 560 Filter
FI15133 600 Filter
FI15134 635 Filter
FI15135 670 Filter
AC16614 Optical bench without filters
AC16615 Reaction rotor cogged belt
AC16616 Reaction rotor motor
AC16617 Rotor centring device
AC16618 Wash station elevation motor
AC16619 Wash station vertical movement belt
AC16620 Reaction rotor temperature sensor
212
List of spares
AC16621 Reaction rotor thermostatting fan assembly (3
wires)
AC16622 Reaction rotor peltier assembly
AC16623 Reaction/sample rotor motor
AC17239 Reaction/sample rotor motor (New model,

see AII to find out the serial number after its
implementation)
AC16624 Reagent rotor cogged belt
AC16625 Barcode reader
AC16626 Refrigerator fan assembly (3 wires)
213
Service manual
List of spares
AC16627 Refrigerator peltier assembly
AC16628 Barcode window heater
AC16629 Barcode reader window
AC16631 Refrigerator temperature sensor
AC16813 Sample rotor centring device
AC16632 Reagent rotor centring device
214
List of spares
AC16391 ISE module
AC17218 Set of ISE module Teflon tubes
AC16825 4 Channel O-Ring Replacement Kit

AC16826 4 Channel O-Ring Electrodes Kit
AC16828 ISE module, flat connection cable
AC16827 ISE module, right angle
AC16829 ISE module, inlet cup
AC16830 ISE module, compression board
AC16831 ISE module board, main board

AC16832 ISE module board, preamplifier
AC16833 Air bubble detector
AC16834 Peristaltic pump assembly
AC16835 Spare clamping device for peristaltic pump (3)
215
Service manual
List of spares
AC16836 Spare clamping device for peristaltic pump (1)
AC17219 Waste tube connector
AC17217 Reagent pack connector
AC16657 Sample rotor cover sensor
AC16791 Inlet filter assembly (5 µm)
AC16792 Spare inlet filter assembly (5 µm)
AC17098 Anionic nitrate filter
AC16815 Full reagent rotor assembly
216
List of spares
AC17097 Water inlet filter
AC17094 Degasser assembly
AC17092 Spare degasser membrane
AC17220 Degasser board
AC17221 Weight with filter (5)
AC17222 Teflon tube with gripper (3 assemblies)
AC17223 Cables for arms (3)
AC17224 Tip arm assembly
AC17225 Stirrer arm assembly
217
Service manual
List of spares
AC17226 Reaction rotor assembly
AC17227 Sample rotor assembly
AC17229 Refrigerator peltier separators (16)
AC17231 Blue, red and green tubes (5 m)

AC17232 Wash station tubes
AC17233 Fluidic connections (20)
AC17234 Fan securing elements (100)
AC17236 Plastic securing elements (24)
AC17254 Ceramic sample pump manifold. (New model,

implementation)
AC17255 Ceramic reagent pump manifold. (New model,

implementation)
218
List of spares
AC17240 Wash station photosensor
AC17259 Manifold assembly. (New model, see AII to find

out the serial number after its implementation)
Old design manifold assembly
AC16641 Accessories box

AC17241 Packing case
219
Service manual
Instruments and tools

AC16643 Adjustment instrument
AC16644 Adjustment screwdriver for level detection
AC16645 Allen key lengthener assembly

AC16824 Set of nylon threads for unblocking tips.
Diameter 0.7 mm and 0.3 mm
AC17212 Gripper tool
AC17213 Frequency measuring device for belt adjustment

AC17242 Air bag
5622 ISE Troubleshooting kit
220
AII. Hardware and software modifications
List of changes in analyser hardware
Serial number Changes

834000271 Added 150 µm filters at distilled water tube inlets.
834000290 In-line degasser assembly incorporated.
834000290 B10 membrane pump model changed. High contamination waste suction pump.
834000390 Change in design of internal distilled water bottle filter body part.
834000341 Y connection of waste tubes replaced by one with a fast connection.
834000409 Change in retainer adapter and elimination of bubble suppressor in washing pump
pistons.
834000434 Change in tension of sample and reagent rotor transmission belt to 55N (5.5 kg).
834000417 Change in plastic separator model supporting the peltiers in the reagent rotor.
834000480, 834000481, Change in ceramic pump subassembly. Each pump has been separated with its
834000482, 834000483, manifold.
834000484, 834000485,
834000486, 834000487,
834000488, 834000489,
from 834000519.
834000480 Incorporation of degasser outlet into the analyser drain.
834000480 Incorporation of the ISE module waste outlet into the high contamination waste
bottle.
834000451 Change in the barcode reader supporting system. Validation of a second barcode
reader source.
834000530 Improvement in assembly of the refrigerator peltier. Incorporation of aerial
connectors into the peltier fans.
834000530 Improvement the transmission assembly in the reagent and sample rotor.
List of changes in software and firmware versions
Date Software Firmware Changes

version version
15/06/2012 1.0 1.0 First version.
20/06/2013 2.0.0 1.20 New ASTM communication protocol for LIS.
15/10/2013 2.1.1 1.26 New HL7 communication protocol for LIS.
Turkish language added.
11/03/2014 2.1.2 1.30 Less bug fixing.
08/05/2014 3.0 2.06 New session pause functionality.
20/06/2014 3.0.1 2.08 Less bug fixing.
10/07/2014 3.0.2 2.10 Less bug fixing and report correction.
02/12/2014 3.1 2.14 New ISE test programming parameters.
Manual resending of results to LIS.
Reordering of tests on selection screen.
Inclusion of new reports on the blanks and standards historical
screen.
221
Service manual
Date Software Firmware Changes

version version
10/2015 3.2 2.20 Possibility of creating tests calculated with ISE and external
techniques. Example of anion gap and creatinine clearance.
Possibility of creating tests with decreasing standard. Example
UIBC.
Compatibility with windows 8.1 and windows 10.
Change in priority and/or type of tube in samples sent by LIS.
Increase in internal quality control memorisation capacity.
Improvement in entering patient data.
Simultaneous reading of sample and reagent rotor barcode.
Automatic recovery of arms after a collision.
Partial deletion of positioned samples that have been finalised.
222
AIII. List of firmware errors
CPU BR1,BR2,BM1,AG1,AG2
0 Without Errors 100 BR1 Vertical Home Error
1 “CPU Firmware Mismatch. 101 BR1 Horizontal Home Error
Update from repository must be done” 102 BR1 Vertical step loss detected
2 “Distributed Firmware Mismatch. 103 BR1 HOME Error in recovery state
Update from repository must be done”
108 BR1 Board doesn't answer
3 FW Repository Error
109 BR1 Board Reset
4 A400 FW Error (Checksums)
110 BR2 Vertical Home Error
5 A400 FW Execution Error
111 BR2 Horizontal Home Error
6 “Maneuvers/Adjustments Mismatch.
112 BR2 Vertical step loss detected
Update from repository must be done”
113 BR2 HOME Error in recovery state
10 CAN Bus Error
118 BR2 Board doesn't answer
20 “Instruction Rejected by a previous Error
(Permanent or Self-recover like covers) (Stand 119 BR2 Board Reset
by-Running)” 120 BM1 Vertical Home Error
21 Instruction Aborted by an Error (Stand by- 121 BM1 Horizontal Home Error
Running) 122 BM1 Vertical step loss detected
22 Recover Failed 123 BM1 HOME Error in recovery state
28 “Impossible to perform TEST/PTEST/ 128 BM1 Board doesn't answer
WRUN;
129 BM1 Board Reset
Wait to next Cycle”
130 AG1 Vertical Home Error
34 “Impossible to perform TEST/PTEST/
WRUN; 131 AG1 Horizontal Home Error
Already received one in the same cycle” 132 AG1 Vertical step loss detected
35 Error in Scheduler Table 133 AG1 HOME Error in recovery state
40 Instruction unknown or with wrong 138 AG1 Board doesn't answer
parameter 139 AG1 Board Reset
41 Instruction Not Allowed on This State 140 AG2 Vertical Home Error
42 Instruction Not Allowed on This Instruction 141 AG2 Horizontal Home Error
43 COMMAND script wrong 142 AG2 Vertical step loss detected
45 LOADADJ Wrong 143 AG2 HOME Error in recovery state
61 ISE Time Out 148 AG2 Board doesn't answer
70 Main Cover opened while instrument is 149 AG2 Board Reset
working
99 Multiple Errors
223
Service manual
DR1,DR2,DM1 GLF
200 DR1 Freq Detection system Error (Frequency 500 Rotor Home Error
Wrong) 501 Rotor Encoder Error
201 DR1 Probe Thermistor Error 502 Rotor Safety Stop: Washing Station Down
202 DR1 Probe Heater Error 503 Photometrics Cover Opened
203 DR1 Collision Detected 504 Washing Station Collision
208 DR1 Board doesn’t answer 510 Photometrics Temperature Alarm
209 DR1 Board Reset 511 Photometrics Peltiers Error
210 DR2 Freq Detection system Error (Frequency 512 Photometrics Fans Error
Wrong)
520 Washing Station Home Error
211 DR2 Probe Thermistor Error
530 Photometric Board not detected
212 DR2 Probe Heater Error
550 Metacrilate Rotor Missing
213 DR2 Collision Detected
598 GLF Board doesn’t answer
218 DR2 Board doesn’t answer
599 GLF Board Reset
219 DR2 Board Reset
220 DM1 Freq Detection system Error
(Frequency Wrong) SFX
223 DM1 Collision Detected Code Description
228 DM1 Board doesn’t answer 600 Washing Station Pump Home Error
229 DM1 Board Reset 610 Washing Station Heater Temperature Alarm
611 Washing Station Heater Error
620 Waste Tank Sensor Impossible Value
RR1, RM1
621 Too much time to empty Waste Tank
Code Description
630 Water Tank Sensor Impossible Value
300 RR1 Home Error
631 Too much time to fill Water Tank
301 RR1 Fridge Temperature Alarm
640 High Contamination Waste Gauge electronics
302 RR1 Fridge Peltiers Error
error
303 RR1 Fridge Fans Error
650 Washing Solution Gauge electronics error
305 RR1 Cover Opened
660 Error detected in B1
310 RR1 Code Bar Reader Error
311 RR1 Code Bar Reader Time Out Error
348 RR1 Board doesn't answer
349 RR1 Board Reset
350 RR1 Home Error
355 RM1 Cover Opened
360 RM1 Code Bar Reader Error
361 RM1 Code Bar Reader Time Out Error
398 RM1 Board doesn't answer
399 RM1 Board Reset
670 Error detected in GE1
671 Error detected in EV1
224
SFX
Code Description
698 SF1 Board doesn't answer
699 SF1 Board Reset
JEX
Code Description
700 Sample Pump Home Error
701 Reagent 1 Pump Home Error
702 Reagent 2 Pump Home Error
710 Clot Detector Hardware Error
711 Sample Fluidics Obstructed
798 JE1 Board doesn’t answer
799 JE1 Board Reset
225
Service manual
AIV. Technical characteristics

The manufacturer declines all liability for damages caused by using the
appliance incorrectly.
WARNING
GENERAL CHARACTERISTICS
Speed 400 prep/h (without electrolytes)
ISE module speed 320 prep/h
Analysis principles Colorimetry, turbidimetry.
ISE module: Potentiometry (selective electrode
method): Na+, K+, Cl- (Li+ is optional)
SAMPLE CONTROL
Sample rotor capacity 135
Barcode detector Yes
Number of samples with barcodes 90
Size of primary tubes Diameter 12 mm to 16 mm (max. height 100 mm)
Sample well Sample well diameter 13.5 mm
Type of sample pump syringe Low-maintenance ceramic piston
Piston diameter 3 mm
Pipetting volume 2 μL to 40 μL
Pipetting resolution 0.1 μL
Maximum ratio between sample and reagent volume 1:2 to 1:200
Level detection Yes
Washing of tip Interior and exterior
Clot sensor Yes
Vertical collision detector Yes
REAGENT CONTROL
Reagent bottle volume 20 mL, 60 mL
Reagent rotor capacity 88 (44 bottles of 20 mL or
60 mL + 44 bottles of 20 mL)
Refrigerated reagents Yes
Maximum refrigerator temperature 5° C to 8° C (at room temperature of 25° C)
Barcode detector Yes
Reagent arms 2 (R1, R2)
R1 reagent volume 90 mL to 450 mL
R2 reagent volume 10 mL to 300 mL
Type of reagent pump syringe Low-maintenance ceramic piston
Piston diameter 8 mm
Pipetting resolution 1 mL
226
REAGENT CONTROL
Level detection Yes
Washing of tip Interior and exterior
Vertical collision detector Yes
Thermostatted tip Yes
REACTION ROTOR
Minimum reaction volume 180 μL
Maximum reaction volume 600 μL
Number of cuvettes 120
Cuvette material UV methacrylate
Type of incubation Dry
Fixed dispensing time for 2nd reagent 5 min (fixed)
Reaction cuvette temperature 37 ℃
Temperature accuracy ±0.2 °C
Temperature stability ±0.1 °C
Stirrers 2
CUVETTE WASHING SYSTEM

Number of washing system tips 7
Number of tips with washing solution 2
Rinsing with water 3
Drying 2
Washing volume 711 μL
Washing solution consumption 1.42 mL/cycle
OPTICAL SYSTEM
Light source LED+Hard Coating Filter
No. of wavelengths 8
Wavelengths 340 – 405 – 505 – 535 – 560 – 600 – 635 – 670
nm
Filter band width 10 nm ± 2 nm
Wavelength accuracy ± 2 nm
Photometric range -0.2 A to 3.5 A
Internal resolution 0.0001
Detector Principal photodiode + reference photodiode
Measurement precision (for 340 nm, 405 nm and CV < 1 % at 0.1 A
505 nm) CV < 0.1 % at 2 A
ISE MODULE (optional)

Sample type Serum, Plasma or Urine
Type of electrode Na+, K+, Cl-. Li+ (optional)
227
Service manual
ISE MODULE (optional)

Sample volume Serum: 100 mL
Urine: 200 mL
ENVIRONMENTAL REQUIREMENTS
Room temperature 10° C to 35° C
10° C to 30° C (with ISE module)
Relative humidity < 85 % with no condensation
Maximum height < 2 500 m
Contamination grade 2
Transportation and storage temperature 0° C to 40° C
Transportation and storage humidity < 85 % with no condensation
DIMENSIONS AND WEIGHT

Dimensions (Width, depth and height) 1200 mm x 720 mm x 1258 mm
Weight 210 kg
ELECTRICITY REQUIREMENTS
Mains voltage 115 V to 230 V
Network frequency 50 Hz or 60 Hz
Electric power 500 VA
FLUID REQUIREMENTS
Water inlet Through external tank or through direct
connection
Type of water Purification type II (NCCLS)
Water consumption < 14 L/h
High contamination waste tank Internal, 5 L
Washing solution tank Internal, 5 L
MINIMUM COMPUTER REQUIREMENTS

Operating system Windows® 7 64 bit (x64), windows 8.1, windows
10
CPU Equivalent to Intel Core i3 @3.10 GHz or higher
RAM memory 4 Gbytes
Hard disk 40 Gbytes or more
DVD player Yes
VGA monitor Minimum resolution 1 024 x 768
Serial channel connector USB
COMPLIANCE WITH DIRECTIVES AND STAND-

ARDS
EC-IVD Directive 98/79/EC
228
AV. Adjustment margin tables
Adjustment margins:
Parameter Minimum Maximum

Reading reference position - Well 1 7 860 7 905
Washing solution tare 0 -5 5

Washing solution tare 50 40 60
Waste tare 0 -5 5
Waste tare 50 40 60
Waste tare 100 95 105
Waste tare 150 100 150
Thermal adjustment of reaction rotor 37.0 39.0

Thermal adjustment of wash station 44.0 50.0
Thermal adjustment of reagent tip 1 45.0 55.0
Thermal adjustment of reagent tip 2 45.0 55.0
Thermal test on reaction rotor 36.8 37.2

Thermal test on wash station 45.0 50.0
Thermal test on reagent tip 1 30.0 35.0
Thermal test on reagent tip 2 30.0 35.0
Led 340 intensity reference value 6 000 25 000

Photometry margins:
Parameter Minimum Maximum

Main photodiode dark current 3 300 4 500
Reference photodiode dark current 3 300 4 500
Number of counts of main photodiode Minimum Maximum

Wavelength 340 600 000 950 000
Wavelength 405 850 000 950 000
229
Service manual
Number of counts of main photodiode Minimum Maximum

Wavelength 505 850 000 950 000
Wavelength 535 850 000 950 000
Wavelength 560 420 000 950 000
Wavelength 600 750 000 950 000
Wavelength 635 850 000 950 000
Wavelength 670 450 000 950 000
Number of counts of reference Minimum Maximum

photodiode
Wavelength 340 100 000 400 000
Wavelength 405 400 000 950 000
Wavelength 505 400 000 950 000
Wavelength 535 400 000 950 000
Wavelength 560 400 000 950 000
Wavelength 600 300 000 950 000
Wavelength 635 400 000 950 000
Wavelength 670 200 000 950 000
Measurements taken at the

Parameter Value
505 nm wavelength
Repeatability measured at 0 Standard
< 0.0002 Abs
Abs deviation
Stability at 0 Abs Range < 0.0015 Abs

230
AVI. Repackaging instructions
This chapter gives the instructions for repackaging and control of the marketed instruments for rental or free lease,
or transferred as second hand.
General terms and conditions

• Useful life: There is no limit on the useful life, provided the manufacturer's instructions regarding use and
maintenance set out in the User Manual are complied with and original spares are used. BioSystems will maintain
the spares service up to 5 years after the manufacture of the same model of the last analyser.
• Number of times the appliance can be repackaged: There is no limit regarding the number of times it can
be repackaged, provided the repackaging instructions described in this section are complied with and original
spares are used.
• Accessories used in repackaged appliances: All repackaged analysers will use the accessories supplied by the
manufacturer. Any remains of surplus fungibles used previously such as rotors, spare reagent or washing solu-
tion bottles, etc. will be disposed of.
• Personnel: The repackaging operation will be carried out by personnel authorised by Biosystems. This person-
nel will normally be the dealer's technical service staff or persons who have received the appropriate training.
Instructions
Cleaning and disinfecting
Users carrying out disinfection processes must wear gloves and protective apparel. All consumable materials (reaction
rotor, reagent bottles, sample tubes) will be treated as potentially infectious waste.
Check whether there are any reaction rotors in their compartments or reagent bottles in the refrigerator. If there
are, dispose of them.
Empty the high contamination waste tank and washing solution tank.
Wash the inside of the equipment using a damp cloth and neutral soap. If there are splashes, wash them with alcohol.
Follow the applicable national guidelines for disposing of waste that is considered potentially infectious.
Components to be replaced
Replace the following components:
• Wash station suction tubes
• Wash station dryer
• Distilled water and washing solution bottle filters
• ISE module peristaltic pump tubes
Update the firmware and software
Adjustment process.
Take note of the values obtained from the adjustment in the record document at the end of the service manual.
Make the following adjustments. To make the adjustments, consult chapter 6 of the service manual.
• Verification of the LED currents
• Thermostatting adjustment in the reagent tips
• Thermostatting adjustment in the reaction rotor
231
Service manual
• Photometry test
formul
a18
fo r mu la 1 9
Execute with the user programme.
To make the functional check, use the following BioSystems reagents and reference materials:
• Glucose reagent (21503)
• AST reagent (21531)
• Multi-standard serum (18011)
• Level I and II control serum (18009 and 18010)
Change the following parameters in the glucose and AST test programmes:
Number of replicates: 20
Multistandard concentration: Enter the value.
Programme the following list:
Two patients called: Level I and Level II
Programme the tests for each patient: Glucose and AST
Execute the list. The analyser will perform the blank, standard and 20 replicates at each control serum level. Once
it has finished it will display the results.
Calculate the CV for each result with the 20 replicates.
The CV is calculated as:
√
n
SD
∑ ( xi − Average)2
i=1
CV = ⋅100 SD=
Average n−1

Check that the results are within the margins:
Biochemical precision CV (%)

Control serum Level I glucose <2
Control serum Level II glucose <2
Control serum level I AST <6
Control serum level II AST <2
Check that the mean of the results for each control serum is within the margins indicated in the value entered in
each control serum.
If the results are not within the above margins, the analyser is damaged. Solve the problem. Once repaired, perform
the functional check again.
Finalisation
Put an indelible label on the repackaged equipment. The label should bear the following information (check the
national legislation in each country):
• The company name and address and the names of the persons responsible for the execution and repackaging
• The date of the last inspection
• The number of repackaging operations carried out
SD = sqrt {sum from i=1 to n (x_i - Average)^2 over {n-1}}

CV = SD over Average cdot 100 232
• An indication that it is a renovated appliance
Useful life: no limit, provided the manufacturer's instructions regarding use and maintenance are followed.
Add a new box of accessories.
Use the original packaging. First check its state and if it is clearly damaged, replace with a new one.
Send the completed record to the Biosystems technical service (in pdf format).
233
REPACKAGING RECORD
Company name: 4.3. Number of counts of reference photodiode

Serial number: 340 nm 100 000 400 000
Repackaging date: 405 nm 400 000 800 000
505 nm 400 000 800 000
PART REPLACEMENT PROCESS 535 nm 400 000 800 000
560 nm 400 000 950 000
1. Parts to be replaced 600 nm 300 000 800 000
Replacement of parts Ok / Not Ok 635 nm 400 000 800 000
Update the firmware 670 nm 200 000 950 000
Ok / Not Ok
and software
4.4. Dark current counts
Value 3 300 4 500
ADJUSTMENT PROCESS
4.5. Repeatability Tolerances CV (%)
For all the filters < 10%
Points to be readjusted Tolerances Value
4.6. Stability
1. Thermostatting of reagent 1 tip
For all the filters < 10%
Temperature set point 50.0 55.0
Temperature test 30.0 35.0
FINAL FUNCTIONAL CHECK
2. Thermostatting of reagent 2 tip
Temperature set point 50.0 55.0
5. Biochemical precision Tolerances CV(%) Value
Temperature test 30.0 35.0
Control serum Level I
3. Thermostatting of reaction rotor <2
Glucose
Temperature set point 37.0 39.0 Control serum Level II
<2
Temperature test 36.8 37.2 Glucose
4. Photometry Tests Control serum level I
<6
4.1. Luminous intensity AST
340 nm Control serum level II
6 000 25 000 <2
AST
405 nm 4 000 30 000
505 nm 5 000 30 000
FINALISATION
535 nm 1 000 30 000
560 nm 2 000 15 000
6. Completed
600 nm 4 000 25 000 Label the renovated Ok / Not Ok
635 nm 4 000 20 000 equipment
670 nm 4 000 15 000 Add a full box of acces- Ok / Not Ok
4.2. Number of counts of main photodiode sories
340 nm 600 000 950 000 Check the packaging Ok / Not Ok
405 nm 850 000 950 000
505 nm 850 000 950 000 7. Acceptance of repackaging
535 nm 850 000 950 000 Repackaging OK
Ok / Not Ok
560 nm 420 000 950 000 Available for sale
600 nm 750 000 950 000
635 nm 850 000 950 000 Signed:
670 nm 450 000 950 000
Prepared by:
Page 1/1 Rev 2.0 Operative date: 30/09/2015

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ENGLISH

Manual code TESE000012-03-ESP

Manufacturer's address BIOSYSTEMS

The BA400 is compliant with EU directive 98/79/EC

4.10. Syringe control board - CIIM00054................................................................................ 75

8.10.3. AC16578 - Wash station electro valve.................................................................... 152

Persons for whom this manual is intended

Notices and warnings

The symbol warns of a potential risk due to laser radiation emission.

Medical device for In Vitro Diagnostics.

Please consult the directions for use.

Abbreviations and units shown in the manual

2.1. Sample rotor

2.2. Reagent rotor

Both 60 mL and 20 mL bottles can be placed in the inner ring.

2.3. Reaction rotor

2.4. Optical system

2.5. Wash station

2.6. Waste, distilled water and washing solution containers

2.7. Stirring arm

2.8. Dispensing arm

• Reagent arm R1: from 90 μL to 450 μL

2.9. ISE module (optional)

2.10. Operating mode

3.1. Sample rotor

Figure 3.1 Sample rotor assembly

Figure 3.2 Detail of sample rotor assembly

Figure 3.3 Barcode reader adjustment

3.2. Reagent rotor

Figure 3.4 Sample rotor assembly

References of figures 3.4 and 3.5:

Figure 3.5 Reagent rotor cut-off

3.3. New barcode reader support design

Part (figures 3.4 and

Figure 3.6 Exploded view of Microscan reader support

Figure 3.7 Exploded view of Opticon reader support

9 Microscan Reagents and samples

Figure 3.8 Microscan reader assembly. Reagents and samples rotor

Figure 3.9 Opticon reader assembly

13 Microscan Reagents and samples. Opticon Reagents

Figure 3.10 Support plate assembly

3.3.1. Reagent rotor reader adjustment

Figure 3.11 Barcode reader distortion adjustment

Figure 3.12 Barcode reader angular and inclination adjustment

Figure 3.13 Checking the barcode reader adjustment

Figure 3.14 Checking the laser beam positioning

Figure 3.15 Checking the laser beam positioning

3.3.2. Sample rotor reader adjustment

3.4. Reaction rotor

Figure 3.20 Reaction rotor assembly

Figure 3.21 Reaction rotor cut-off

Figure 3.22 Reaction rotor transmission assembly

References of Figure 3.20, Figure 3.21, and Figure 3.22:

Figure 3.23 Wash station tip assembly

Figure 3.24 Wash station elevation system

References of Figure 3.23, and Figure 3.24:

Figure 3.25 Optical bench view

Figure 3.26 Optical bench view

3.5. Pipetting arms

Figure 3.27 Pipetting arm assembly

Figure 3.28 Reagent tip

References of Figure 3.27 and Figure 3.28:

Reagent tip Sample tip

3.6. Stirring arm