Svoboda | Graniru | BBC Russia | Golosameriki | Facebook

Measles virus suppresses RIG-I-like receptor activation in dendritic cells via DC-SIGN-mediated inhibition of PP1 phosphatases

Cell Host Microbe. 2014 Jul 9;16(1):31-42. doi: 10.1016/j.chom.2014.06.008.

Abstract

Dendritic cells (DCs) are targets of measles virus (MV) and play central roles in viral dissemination. However, DCs express the RIG-I-like receptors (RLRs) RIG-I and Mda5 that sense MV and induce type I interferon (IFN) production. Given the potency of this antiviral response, RLRs are tightly regulated at various steps, including dephosphorylation by PP1 phosphatases, which induces their activation. We demonstrate that MV suppresses RIG-I and Mda5 by activating the C-type lectin DC-SIGN and inducing signaling that prevents RLR dephosphorylation. MV binding to DC-SIGN leads to activation of the kinase Raf-1, which induces the association of PP1 inhibitor I-1 with GADD34-PP1 holoenzymes, thereby inhibiting phosphatase activity. Consequently, GADD34-PP1 holoenzymes are unable to dephosphorylate RIG-I and Mda5, hence suppressing type I IFN responses and enhancing MV replication. Blocking DC-SIGN signaling allows RLR activation and suppresses MV infection of DCs. Thus, MV subverts DC-SIGN to control RLR activation and escape antiviral responses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion Molecules / metabolism*
  • Cell Line
  • DEAD Box Protein 58
  • DEAD-box RNA Helicases / metabolism*
  • Dendritic Cells / immunology*
  • Dendritic Cells / virology
  • Host-Pathogen Interactions*
  • Humans
  • Immune Evasion
  • Lectins, C-Type / metabolism*
  • Measles virus / immunology*
  • Measles virus / physiology
  • Protein Phosphatase 1 / antagonists & inhibitors*
  • Receptors, Cell Surface / metabolism*
  • Receptors, Immunologic

Substances

  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Lectins, C-Type
  • Receptors, Cell Surface
  • Receptors, Immunologic
  • Protein Phosphatase 1
  • RIGI protein, human
  • DEAD Box Protein 58
  • DEAD-box RNA Helicases