Evaluation of Anti-Infection and Anti-Diabetic Activities in Methanolic and n-Hexane Plant Extracts of Indigenously Cultivated Chenopodium album
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Microbial Strains
2.3. Collection and Identification of Plant
2.4. Preparation of Plant Extracts
2.5. Mineral Analysis of C. album
2.6. Alpha-Amylase Inhibitory Assay
2.7. Urease Inhibitory Assay
2.8. Antibacterial Activity
2.9. Statistical Analysis
3. Results and Discussion
3.1. Yield of Extract
3.2. Mineral Profile
3.3. Alpha-Amylase Inhibitory Activity
3.4. Urease Inhibitory Activity
3.5. Antibacterial Activity
3.5.1. E. coli Growth Inhibition Activity
3.5.2. S. aureus Growth Inhibition Activity
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Michel, J.; Abd Rani, N.Z.; Husain, K. A review on the potential use of medicinal plants from Asteraceae and Lamiaceae plant family in cardiovascular diseases. Front. Pharmacol. 2020, 11, 852. [Google Scholar] [CrossRef] [PubMed]
- Vaou, N.; Stavropoulou, E.; Voidarou, C.; Tsigalou, C.; Bezirtzoglou, E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms 2021, 10, 2041. [Google Scholar] [CrossRef] [PubMed]
- Tang, K.W.; Millar, B.C.; Moore, J.E. Antimicrobial resistance (AMR). Br. J. Biomed. Sci. 2023, 80, 11387. [Google Scholar] [CrossRef] [PubMed]
- Murray, C.J.; Ikuta, K.S.; Sharara, F.; Swetschinski, L.; Aguilar, G.R.; Gray, A.; Han, C.; Bisignano, C.; Rao, P.; Wool, E.; et al. Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis. Lancet 2022, 10325, 629–655. [Google Scholar] [CrossRef] [PubMed]
- Lobo, V.; Patil, A.; Phatak, A.; Chandra, N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn. Rev. 2010, 4, 118–126. [Google Scholar] [CrossRef] [PubMed]
- Martemucci, G.; Costagliola, C.; Mariano, M.; D’andrea, L.; Napolitano, P.; D’Alessandro, A.G. Free Radical Properties, Source and Targets, Antioxidant Consumption and Health. Oxygen 2022, 2, 48–78. [Google Scholar] [CrossRef]
- Naqvi, S.A.R.; Ali, S.; Sherazi, T.A.; Haq, A.U.; Saeed, M.; Sulman, M. Antioxidant, Antibacterial, and Anticancer Activities of Bitter Gourd Fruit Extracts at Three Different Cultivation Stages. J. Chem. 2020, 2020, 7394751. [Google Scholar] [CrossRef]
- Chaudhary, P.; Janmeda, P.; Docea, A.O.; Yeskaliyeva, B.; AbdullRazis, A.F.; Modu, B.; Calina, D.; Sharifi-Rad, J. Oxidative stress, free radicals and antioxidants: Potential crosstalk in the pathophysiology of human diseases. Front. Chem. 2023, 11, 1158198. [Google Scholar] [CrossRef] [PubMed]
- Giles, G.I.; Jacob, C. Reactive sulfur species: An emerging concept in oxidative stress. J. Biol. Chem. 2002, 383, 375–388. [Google Scholar] [CrossRef]
- Grusak, M.A.; Dellapenna, D. Improving the nutrient composition of plants to enhance human nutrition and health. Annu. Rev. Plant Biol. 1999, 50, 133–161. [Google Scholar] [CrossRef]
- Shah, A.A.; Gupta, A. Antioxidants in health and disease with their capability to defend pathogens that attack apple species of Kashmir. In Plant Antioxidants and Health; Springer: Berlin/Heidelberg, Germany, 2020; pp. 1–26. [Google Scholar]
- Yen, T.S.; Htet, M.K.; Lukito, W.; Bardosono, S.; Setiabudy, R.; Basuki, E.S. Increased vegetable intake improves glycaemic control in adults with type 2 diabetes mellitus: A clustered randomised clinical trial among Indonesian white-collar workers. J. Nutr. Sci. 2022, 11, e49. [Google Scholar] [CrossRef] [PubMed]
- Rasheed, M.U.; Naqvi, S.A.R.; Al-Asmari, F.; Rahim, M.A.; Ramadan, M.F. Phytochemicals, Health-Promoting Effects, and Enzyme Inhibition Traits of Phlomis stewartii Extracts. Molecules 2024, 29, 1049. [Google Scholar] [CrossRef] [PubMed]
- Poonia, A.; Upadhayay, A. Chenopodium album Linn: Review of nutritive value and biological properties. J. Food Sci. Technol. 2015, 52, 3977–3985. [Google Scholar] [CrossRef] [PubMed]
- Singh, S.; Singh, A.; Hallan, S.S.; Brangule, A.; Kumar, B.; Bhatia, R. A compiled update on nutrition, phytochemicals, processing effects, analytical testing and health effects of Chenopodium album: A non-conventional edible plant (NCEP). Molecules 2023, 28, 4902. [Google Scholar] [CrossRef] [PubMed]
- Vijay, N.; Padmaa, M. Hepatoprotective activity of Chenopodium album Linn. against paracetamol induced liver damage. Pharmacologyonline 2011, 3, 312–328. [Google Scholar]
- Rady, M.M.; Belal, H.E.; Gadallah, F.M.; Semida, W.M. Selenium application in two methods promotes drought tolerance in Solanumlycopersicum plant by inducing the antioxidant defense system. Sci. Hortic. 2020, 266, 109290. [Google Scholar] [CrossRef]
- Etsassala, N.G.; Badmus, J.A.; Marnewick, J.L.; Iwuoha, E.I.; Nchu, F.; Hussein, A.A. Alpha-glucosidase and alpha-amylase inhibitory activities, molecular docking, and antioxidant capacities of Salvia aurita constituents. Antioxidants 2020, 9, 1149. [Google Scholar] [CrossRef] [PubMed]
- Biglar, M.; Soltani, K.; Nabati, F.; Bazl, R.; Mojab, F.; Amanlou, M. A preliminary investigation of the jack-bean urease inhibition by randomly selected traditionally used herbal medicine. Iran. J. Pharm. Res. 2012, 11, 831. [Google Scholar] [PubMed]
- Weatherburn, M.W. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 1967, 39, 971–974. [Google Scholar] [CrossRef]
- Amin, M.; Anwar, F.; Naz, F.; Mehmood, T.; Saari, N. Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 2013, 2, 2135–2149. [Google Scholar] [CrossRef]
- Naqvi, S.A.R.; Waseem, R.; Mahmood, N.; Hussain, Z.; Khan, Z.A.; Shahzad, S.A.; Yar, M.; Amin, R.; Bukhari, S.A.; Zahoor, A.F.; et al. Phenolic acid content, antioxidant properties, and antibacterial potential of flowers and fruits from selected pakistani indigenous medicinal plants. Sci. Asia 2013, 9, 340–345. [Google Scholar] [CrossRef]
- Borkowski, A. An easy, convenient statistical method to analyze bark beetle populations. J. For. Res. 2023, 34, 2027–2040. [Google Scholar] [CrossRef]
- El Mannoubi, I. Impact of different solvents on extraction yield, phenolic composition, in vitro antioxidant and antibacterial activities of deseeded opuntiastricta fruit. J. Umm Al-Qura Univ. Appl. Sci. 2023, 9, 176–184. [Google Scholar] [CrossRef]
- Lapornik, B.; Prošek, M.; Wondra, A.G. Comparison of extracts prepared from plant by-products using different solvents and extraction time. J. Food Eng. 2005, 71, 214–222. [Google Scholar] [CrossRef]
- Hassan, L.G.; Umar, K.J. Nutritional value of Balsam Apple (Momordica balsamina L.) leaves. Pak. J. Nutr. 2006, 5, 522–529. [Google Scholar] [CrossRef]
- Celep, E.; Seven, M.; Akyüz, S.; İnan, Y.; Yesilada, E. Influence of extraction method on enzyme inhibition, phenolic profile and antioxidant capacity of Sideritis trojana Bornm. S. Afr. J. Bot. 2019, 121, 360–365. [Google Scholar] [CrossRef]
- Khair-ul-Bariyah, S.; Ahmed, D.; Aujla, M.I. Comparative analysis of Ocimum basilicum and Ocimum sanctum: Extraction techniques and urease and alpha-amylase inhibition. Pak. J. Chem. 2012, 2, 134–141. [Google Scholar] [CrossRef]
- Onoda, Y.; Iwasaki, H.; Magaribuchi, T.; Tamaki, H. Effects of the new anti-ulcer agent 12-sulfodehydroabietic acid monosodium salt on healing of acetic acid-induced gastric ulcers in rats. Arzneimittel-Forschung 1991, 41, 546–548. [Google Scholar]
- Sikri, N.; Dhanda, S.; Dalal, S. Kinetics of urease inhibition by different fractions of Cassia fistula. S. Afr. J. Bot. 2019, 120, 274–279. [Google Scholar] [CrossRef]
- Naqvi, S.A.R.; Khan, Z.A.; Hussain, Z.; Shahzad, S.A.; Yar, M.; Ghaffar, A.; Mahmood, N.; Kousar, S. Antioxidant, antibacterial and antiproliferative activities of areal parts of swertia chirata (bush ham) plant extracts using in vitro models. Asian J. Chem. 2013, 25, 5448–5452. [Google Scholar] [CrossRef]
- Pandey, S.; Gupta, R.K. Screening of nutritional, phytochemical, antioxidant and antibacterial activity of Chenopodium album (Bathua). J. Pharmacogn. Phytochem. 2014, 3, 1–9. [Google Scholar]
- Sarmiento, W.C.; Maramba, C.C.; Gonzales, M.L.M. An in-vitro study on the antibacterial effect of neem (Azadirachta indica) leaf extract on methicillin-sensitive and Methicillin-resistant S. aureus. PIDSP J. 2011, 12, 40–45. [Google Scholar]
Run | Factors | Yield (g/100 g of Dry wt.) | ||||
---|---|---|---|---|---|---|
Methanolic Extracts | n-Hexane Extracts | Solvent Concentration (mL) | Speed (rpm) | Time (Hours) | Methanolic Extracts | n-Hexane Extracts |
M1 | H1 | 100 | 200 | 2 | 10.12 ± 0.34 * | 1.14 ± 0.12 * |
M2 | H2 | 100 | 200 | 5 | 12.41 ± 0.41 * | 1.50 ± 0.21 * |
M3 | H3 | 100 | 200 | 8 | 12.47 ± 0.37 * | 1.53 ± 0.09 * |
M4 | H4 | 200 | 200 | 2 | 10.42 ± 0.04 ** | 1.26 ± 0.15 * |
M5 | H5 | 200 | 200 | 5 | 12.55 ± 0.44 * | 1.60 ± 0.59 ns |
M6 | H6 | 200 | 200 | 8 | 12.72 ± 0.36 * | 2.09 ± 0.29 * |
M7 | H7 | 100 | 100 | 2 | 10.11 ± 0.39 * | 1.15 ± 0.03 * |
M8 | H8 | 100 | 100 | 5 | 12.35 ± 0.53 ns | 1.52 ± 0.24 * |
M9 | H9 | 100 | 100 | 8 | 12.44 ± 0.34 * | 1.54 ± 0.22 * |
M10 | H10 | 150 | 200 | 5 | 12.42 ± 0.28 * | 1.51 ± 0.17 * |
M11 | H11 | 200 | 150 | 5 | 12.57 ± 0.27 * | 1.59 ± 0.21 * |
M12 | H12 | 150 | 150 | 5 | 12.36 ± 0.01 ** | 1.50 ± 0.16 * |
Sr. No | Mineral Profile with Peak Intensity | Values in ppm (µg/g) |
---|---|---|
1 | Zinc (Zn213.8) | 0.12 ± 0.014 |
2 | Cadmium (Cd226.5) | 20.29 ± 1.9 |
3 | Lead (Pb283.3) | 0.75 ± 0.057 |
4 | Chromium (Cr267.7) | 0.20 ± 0.023 |
5 | Magnesium (Mg279.5) | 3546.23 ± 14.01 |
6 | Nickel (Ni361.9) | 0.62 ± 0.047 |
7 | Manganese (Mn257.6) | 0.13 ± 0.015 |
8 | Silicon (Si251.6) | 3.59 ± 0.43 |
9 | Iron (Fe259.9) | 3413.36 ± 12.49 |
Methanolic Extract | Zone of Inhibition (mm) | n-Hexane Extract | Zone of Inhibition (mm) | ||
---|---|---|---|---|---|
S. aureus | E. coli | S. aureus | E. coli | ||
M1 | 12.33 ± 0.04 ** | 14.12 ± 0.38 * | H1 | 12.87 ± 0.55 ns | 13.55 ± 0.57 ns |
M2 | 14.13 ± 0.55 ns | 14.34 ± 0.64 ns | H2 | 13.35 ± 0.55 ns | 13.50 ± 0.25 * |
M3 | 15.37 ± 0.27 * | 15.19 ± 0.01 ** | H3 | 14.26 ± 0.27 * | 14.33 ± 0.53 ns |
M4 | 12.34 ± 0.57 ns | 13.21 ± 0.55 ns | H4 | 13.05 ± 0.59 ns | 13.48 ± 0.54 ns |
M5 | 15.36 ± 0.42 * | 16.10 ± 0.56 ns | H5 | 14.80 ± 0.32 * | 15.46 ± 0.02 ** |
M6 | 15.55 ± 0.55 ns | 16.55 ± 0.57 ns | H6 | 15.14 ± 0.57 ns | 15.70 ± 0.56 ns |
M7 | 10.33 ± 0.60 ns | 11.98 ± 0.60 ns | H7 | 12.92 ± 0.61 ns | 13.26 ± 0.53 ns |
M8 | 13.33 ± 0.61 ns | 13.21 ± 0.57 ns | H8 | 15.05 ± 0.01 ** | 14.04 ± 0.34 * |
M9 | 15.32 ± 0.45 * | 14.54 ± 0.02 ** | H9 | 14.48 ± 0.52 ns | 14.94 ± 0.44 * |
M10 | 14.22 ± 0.52 ns | 14.33 ± 0.52 ns | H10 | 13.91 ± 0.57 ns | 13.73 ± 0.56 ns |
M11 | 15.47 ± 0.55 ns | 16.25 ± 0.25 * | H11 | 14.59 ± 0.03 ** | 15.27 ± 0.35 * |
M12 | 13.29 ± 0.58 ns | 13.85 ± 0.60 ns | H12 | 13.14 ± 0.63 ns | 14.17 ± 0.54 ns |
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Hussain, I.; Janjua, M.R.S.A.; Haq, A.U.; Hassan, S.U.; Albaqami, F.M.K.; Alsuwat, M.A.; Alrashdi, B.M.; Alzwain, S.; Naqvi, S.A.R. Evaluation of Anti-Infection and Anti-Diabetic Activities in Methanolic and n-Hexane Plant Extracts of Indigenously Cultivated Chenopodium album. Agronomy 2024, 14, 1340. https://doi.org/10.3390/agronomy14071340
Hussain I, Janjua MRSA, Haq AU, Hassan SU, Albaqami FMK, Alsuwat MA, Alrashdi BM, Alzwain S, Naqvi SAR. Evaluation of Anti-Infection and Anti-Diabetic Activities in Methanolic and n-Hexane Plant Extracts of Indigenously Cultivated Chenopodium album. Agronomy. 2024; 14(7):1340. https://doi.org/10.3390/agronomy14071340
Chicago/Turabian StyleHussain, Ijaz, Muhammad Ramzan Saeed Ashraf Janjua, Atta Ul Haq, Sadaf Ul Hassan, Faisal Miqad K. Albaqami, Meshari A. Alsuwat, Barakat M. Alrashdi, Sarah Alzwain, and Syed Ali Raza Naqvi. 2024. "Evaluation of Anti-Infection and Anti-Diabetic Activities in Methanolic and n-Hexane Plant Extracts of Indigenously Cultivated Chenopodium album" Agronomy 14, no. 7: 1340. https://doi.org/10.3390/agronomy14071340