Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Experimental Design
2.3. Ultrasound Examinations and Breeding Management
2.4. Embryo Flushing
2.5. Embryo Transfer
2.6. Pregnancy Diagnosis
2.7. Bacteriology
2.8. Blood Samples and Progesterone Assay
2.9. Statistical Analyses
3. Results
3.1. Experiment 1
3.2. Experiment 2
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Betteridge, K.J.; Renard, A.; Goff, A.K. Uterine prostaglandin release relative to embryo collection, transfer procedures and maintenance of the corpus luteum. Equine Vet. J. 1985, 17, 25–33. [Google Scholar] [CrossRef]
- Sirois, J.; Betteridge, K.J.; Goff, A.K. PGF-2 alpha release, progesterone secretion and conceptus growth associated with successful and unsuccessful transcervical embryo transfer and reinsertion in the mare. J. Reprod. Fertil. Suppl. 1987, 35, 419–427. [Google Scholar] [PubMed]
- Choi, Y.H.; Gustafson-Seabury, A.; Velez, I.C.; Hartman, D.L.; Bliss, S.; Riera, F.L.; Roldán, J.E.; Chowdhary, B.; Hinrichs, K. Viability of equine embryos after puncture of the capsule and biopsy for preimplantation genetic diagnosis. Reproduction 2010, 140, 893–902. [Google Scholar] [CrossRef] [PubMed]
- Herrera, C. Clinical Applications of Preimplantation Genetic Testing in Equine, Bovine, and Human Embryos. J. Equine Vet. Sci. 2016, 41, 29–34. [Google Scholar] [CrossRef]
- Riera, F.L.; Roldán, J.E.; Espinosa, J.M.; Fernandez, J.E.; Ortiz, I.; Hinrichs, K. Application of embryo biopsy and sex determination via polymerase chain reaction in a commercial equine embryo transfer program in Argentina. Reprod. Fertil. Dev. 2019, 31, 1917–1925. [Google Scholar] [CrossRef] [PubMed]
- Stout, T.A. Embryo-maternal communication during the first 4 weeks of equine pregnancy. Theriogenology 2016, 86, 349–354. [Google Scholar] [CrossRef] [PubMed]
- Stout, T.A. Equine embryo transfer: Review of developing potential. Equine Vet. J. 2006, 38, 467–478. [Google Scholar] [CrossRef] [PubMed]
- Hinrichs, K. Assisted reproductive techniques in mares. Reprod. Domest. Anim. 2018, 53 (Suppl. S2), 4–13. [Google Scholar] [CrossRef] [PubMed]
- McKinnon, A.O.; Squires, E.L. Equine embryo transfer. Vet. Clin. N. Am. Equine Pract. 1988, 4, 305–333. [Google Scholar] [CrossRef]
- Hartman, D.L. Embryo transfer: Donor mare management after the flush. In Equine Reproduction, 2nd ed.; McKinnon, A.O., Squiers, E.L., Vaala, W.E., Varner, D.D., Eds.; Wiley-Blackwell: Oxford, UK, 2011; pp. 2878–2879. [Google Scholar]
- Dordas-Perpinya, M.; Bruyas, J.F. Practical aspects of equine embryo transfer. Transl. Res. Vet. Sci. 2019, 2, 23–39. [Google Scholar] [CrossRef]
- Martínez-Boví, R.; Sala-Ayala, L.; Querol-Paajanen, A.; Plaza-Dávila, M.; Cuervo-Arango, J. Effects of repeated embryo flushing without PGF2α administration on luteal function, percentage of unwanted pregnancy and subsequent fertility in mares. Equine Vet. J. 2024. online ahead of print. [Google Scholar] [CrossRef]
- Handler, J.; Hoffmann, D.; Weber, F.; Schams, D.; Aurich, C. Oxytocin does not contribute to the effects of cervical dilation on progesterone secretion and embryonic development in mares. Theriogenology 2006, 66, 1397–1404. [Google Scholar] [CrossRef]
- Rubio, M.S.; Allen, W.R.; Wilsher, S.; Mouguelar, H. Effects of embryo flushing on serum progesterone levels and the inter-ovulatory period in Arabian donor mares. J. Equine Vet. Sci. 2023, 125, 103. [Google Scholar] [CrossRef]
- Squires, E.L.; Imel, K.J.; Iuliano, M.F.; Shideler, R.K. Factors affecting reproductive efficiency in an equine embryo transfer programme. J. Reprod. Fert. 1982, 32, 409–414. [Google Scholar]
- Petit, D.L.; Tainturier, D.; Palmer, E. Luteolysis and bacterial contamination associated with unsuccessful cervical embryo transfer in the mare. Theriogenology 1988, 29, 285. [Google Scholar] [CrossRef]
- Ginther, O.J. Ultrasonic Imaging and Animal Reproduction: Horses; Equiservices Publishing: Cross Plains, WI, USA, 1995. [Google Scholar]
- Samper, J.C. Uterine edema in the mare. In Equine Breeding Management and Artificial Insemination, 2nd ed.; Elsevier: Amsterdam, The Netherlands, 2009; pp. 133–138. [Google Scholar] [CrossRef]
- Samper, J.C. Ultrasonographic appearance and the pattern of uterine edema to time ovulation in mares. Proc. Annu. Conv. Am. Equine 1997, 43, 189–191. [Google Scholar]
- Cuervo-Arango, J.; Newcombe, J.R. Repeatability of preovulatory follicular diameter and uterine edema pattern in two consecutive cycles in the mare and how they are influenced by ovulation inductors. Theriogenology 2008, 69, 681–687. [Google Scholar] [CrossRef]
- Pierson, R.A.; Ginther, O.J. Ultrasonic evaluation of the corpus luteum of the mare. Theriogenology 1985, 23, 795–806. [Google Scholar] [CrossRef]
- McKinnon, A.O.; Squires, E.L. Morphology assessment of the equine embryo. J. Am. Vet. Med. Assoc. 1988, 192, 401–406. [Google Scholar]
- Cuervo-Arango, J.; Martín-Peláez, M.S.; Claes, A.N. A practical guide to estimate the age of the early CL by ultrasonography in mares examined for the first time to be used as recipients in a commercial embryo transfer program. Theriogenology 2020, 153, 48–53. [Google Scholar] [CrossRef]
- Cuervo-Arango, J.; Claes, A.N.; Stout, T.A. Effect of embryo transfer technique on the likelihood of pregnancy in the mare: A comparison of conventional and Wilsher’s forceps-assisted transfer. Vet. Rec. 2018, 183, 323. [Google Scholar] [CrossRef] [PubMed]
- Ginther, O.J. Prolonged luteal activity in mares—A semantic quagmire. Equine Vet. J. 1990, 22, 152–156. [Google Scholar] [CrossRef] [PubMed]
- Allen, W.R. Luteal deficiency and embryo mortality in the mare. Reprod. Domest. Anim. 2001, 36, 121–131. [Google Scholar] [CrossRef] [PubMed]
- Brogan, P.T.; Henning, H.; Stout, T.A.; de Ruijter-Villani, M. Relationship between colour flow Doppler sonographic assessment of corpus luteum activity and progesterone concentrations in mares after embryo transfer. Anim. Reprod. Sci. 2016, 166, 22–27. [Google Scholar] [CrossRef] [PubMed]
- Hayes, M.A.; Quinn, B.A.; Keirstead, N.D.; Katavolos, P.; Waelchli, R.O.; Betteridge, K.J. Proteins associated with the early intrauterine equine conceptus. Reprod. Domest. Anim. 2008, 43 (Suppl. S2), 232–237. [Google Scholar] [CrossRef] [PubMed]
- Ellenberger, C.; Wilsher, S.; Allen, W.R.; Hoffmann, C.; Kölling, M.; Bazer, F.W.; Klug, J.; Schoon, D.; Schoon, H.A. Immunolocalisation of the uterine secretory proteins uterocalin, uteroferrin and uteroglobin in the mare’s uterus and placenta throughout pregnancy. Theriogenology 2008, 70, 746–757. [Google Scholar] [CrossRef] [PubMed]
- Ferris, R.A. Endometritis: Diagnostic Tools for Infectious Endometritis. Vet. Clin. N. Am. Equine Pract. 2016, 32, 481–498. [Google Scholar] [CrossRef]
- Oquendo, P.S.; Silva, E.S.M.; Oquendo, F.M.G.; Cuervo-Arango, J.; Beletti, M.E. The effect of priming and duration of oestradiol benzoate treatment before progesterone administration on embryo development and survival in anestrous recipient mares. Reprod. Domest. Anim. 2022, 57, 1474–1477. [Google Scholar] [CrossRef]
Group | n | P4 0 h (ng/mL) | P4 72 h (ng/mL) | P4 Difference (ng/mL) | Free IUF by 72 h (%) | Pregnancy after ET (%) | IOI (Min–Max) |
---|---|---|---|---|---|---|---|
EF | 8 | 6.7 ± 2.3 | 5.9 ± 1.2 a,b | −0.8 ± 1.3 | 12.5 | - | 22.5 ± 1.3 (20–24) |
EF-ET | 8 | 6.0 ± 1.2 | 3.9 ± 2.3 a | −2.1 ± 2.7 * | 50.0 | 0.0 a | 21.7 ± 4.1 (17–29) |
ET | 8 | 6.5 ± 1.4 | 6.1 ± 1.3 b | −0.4 ± 1.5 | 0.0 | 75.0 b | - |
Group | n | Embryo Size at ET | Positive Recoveries | Fold Increase in Size by 24 h | Grade 1–2 Embryos | Grade 3–4 Embryos | Recovery of Capsule Fragments |
---|---|---|---|---|---|---|---|
EF-ET | 11 | 767 ± 456 | 7/11 63.6 | 1.6 ± 0.8 a | 3/7 42.9 a | 4/7 57.1 a | 3/11 27.3 |
ET | 6 | 630 ± 579 | 5/6 83.3 | 2.1 ± 0.4 b | 5/5 100.0 b | 0/5 0.0 b | 1/6 16.7 |
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Martínez-Boví, R.; Gaber, J.T.H.; Sala-Ayala, L.; Plaza-Dávila, M.; Cuervo-Arango, J. Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares. Animals 2024, 14, 1605. https://doi.org/10.3390/ani14111605
Martínez-Boví R, Gaber JTH, Sala-Ayala L, Plaza-Dávila M, Cuervo-Arango J. Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares. Animals. 2024; 14(11):1605. https://doi.org/10.3390/ani14111605
Chicago/Turabian StyleMartínez-Boví, Rebeca, Jana T. H. Gaber, Laura Sala-Ayala, María Plaza-Dávila, and Juan Cuervo-Arango. 2024. "Pregnancy and Luteal Responses to Embryo Reinsertion following Embryo Flushing in Donor Mares" Animals 14, no. 11: 1605. https://doi.org/10.3390/ani14111605