Anti-Inflammatory Effect of Atorvastatin and Rosuvastatin on Monosodium Urate-Induced Inflammation through IL-37/Smad3-Complex Activation in an In Vitro Study Using THP-1 Macrophages
Abstract
:1. Introduction
2. Results
2.1. IL-37 Expression in MSU-Induced Inflammation
2.2. Effect of Statins on Caspase-1, IL-1β, and IL-37 Expression in the MSU-Induced Inflammatory Response
2.3. Effects of Statins on Interactions with Smad3 and IL-37 in MSU-Induced Inflammation
2.4. Effect of Statins on Proteolytic Activity in the Process of IL-37 Activation
3. Discussion
4. Materials and Methods
4.1. Study Population and Collecting Blood Samples
4.2. Cell Culture
4.3. Chemicals and Antibodies
4.4. Enzyme-Linked Immunosorbent Assay (ELISA)
4.5. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.6. Transfection of siRNA
4.7. Western Blotting
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, S.-K.; Choe, J.-Y.; Kim, J.-W.; Park, K.-Y.; Kim, B. Anti-Inflammatory Effect of Atorvastatin and Rosuvastatin on Monosodium Urate-Induced Inflammation through IL-37/Smad3-Complex Activation in an In Vitro Study Using THP-1 Macrophages. Pharmaceuticals 2024, 17, 883. https://doi.org/10.3390/ph17070883
Kim S-K, Choe J-Y, Kim J-W, Park K-Y, Kim B. Anti-Inflammatory Effect of Atorvastatin and Rosuvastatin on Monosodium Urate-Induced Inflammation through IL-37/Smad3-Complex Activation in an In Vitro Study Using THP-1 Macrophages. Pharmaceuticals. 2024; 17(7):883. https://doi.org/10.3390/ph17070883
Chicago/Turabian StyleKim, Seong-Kyu, Jung-Yoon Choe, Ji-Won Kim, Ki-Yeun Park, and Boyoung Kim. 2024. "Anti-Inflammatory Effect of Atorvastatin and Rosuvastatin on Monosodium Urate-Induced Inflammation through IL-37/Smad3-Complex Activation in an In Vitro Study Using THP-1 Macrophages" Pharmaceuticals 17, no. 7: 883. https://doi.org/10.3390/ph17070883