UV-B Radiation Enhances Epimedium brevicornu Maxim. Quality by Improving the Leaf Structure and Increasing the Icaritin Content
Abstract
:1. Introduction
2. Results
2.1. Effects of UV-B Radiation on the Tissue Structure and Secondary Metabolites of E. brevicornu Leaves
2.2. Analysis of Metabolites during Radiation Exposure
2.3. Analysis of Differential Metabolites
2.4. Metabolites Related to E. brevicornu Quality Traits in the Three Treatment Groups
3. Discussion
3.1. Effect of UV-B Radiation on the Leaf Structure
3.2. Effect of UV-B Radiation on Secondary Metabolites of E. brevicornu
4. Materials and Methods
4.1. Plant Materials
4.2. Measurement of the Leaf Length, Width and Area
4.3. Observation of the Leaf Microstructure
4.4. Observation of the Chloroplast Ultrastructure
4.5. Sample Extraction and HPLC Conditions
4.6. Widely Targeted Metabolomics
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Appendix A
Related Factors | Regression Equation | Multiple Correlation Coefficient | Mean Absolute Deviation |
---|---|---|---|
X1, X2 | Y = 17.962X1 + 27.679X2 − 653.772 | 0.986 | |
X1, X3 | Y = 3.921X1 + 0.66X3 − 66.676 | 0.997 | 18.942 |
X1, X4 | Y = −9.716X1 + 27.679X4 − 653.772 | 0.986 | |
X2, X3 | Y = −4.681X2 + 0.795X3 + 58.728 | 0.997 | 19.094 |
X2, X4 | Y = 9.716X2 + 17.962X4 − 653.772 | 0.986 | |
X3, X4 | Y = 0.672X3 + 1.723X4 − 56.964 | 0.997 | 16.415 |
X1, X2, X3 | Y = 3.745X1 − 4.256X2 + 0.725X3 − 4.648 | 0.997 | 21.586 |
X2, X3, X4 | Y = −8X2 + 0.725X3 + 3.745X4 − 4.648 | 0.997 | 21.586 |
Flavonols | Regression Equation | R−2 | Linear Range (μg/mL) |
---|---|---|---|
Epimedin A | Y = 15.902X − 33.942 | 0.9992 | 10–200 |
Epimedin B | Y = 13.412X − 25.350 | 0.9995 | 20–400 |
Epimedin C | Y = 8.107X + 14.032 | 0.9999 | 20–600 |
Icariin | Y = 12.640X + 38.156 | 0.9991 | 10–200 |
Icariside I | Y = 3.039X − 0.889 | 0.9998 | 5–40 |
Icariside II | Y = 12.270X + 4.448 | 0.9998 | 1–16 |
Type | Number | Percentage (%) |
---|---|---|
Flavonols | 117 | 38.49 |
Flavones | 104 | 34.21 |
Flavanones | 21 | 6.91 |
Isoflavones | 17 | 5.59 |
Flavonoid carbonoside | 12 | 3.95 |
Flavanols | 12 | 3.95 |
Flavanonols | 11 | 3.62 |
Other Flavonoids | 5 | 1.64 |
Chalcones | 4 | 1.32 |
Dihydroisoflavones | 1 | 0.33 |
Compounds | Classification | UV-B 0 | UV-B 10 | UV-B 20 | Log2FC (UV-B 0 vs. UV-B 10) | Log2FC (UV-B 0 vs. UV-B 20) | Log2FC (UV-B 10 vs. UV-B 20) | K-Means |
---|---|---|---|---|---|---|---|---|
Baohuoside II | Flavonoids | − | − | 4 | ||||
Baohuoside III | Flavonoids | − | − | 4 | ||||
Ikarisoside F | Flavonoids | − | − | 4 | ||||
7-O-Methylnaringenin | Flavonoids | 4 | ||||||
3,4,5-Trimethoxyphenol | Phenolic acids | 1 | ||||||
Dehydrodiconiferyl alcohol | Phenolic acids | 1 | ||||||
Epipinoresinol | Lignans | 1 | ||||||
3,4-dihydroxy-allylbenzene-3 | Others | 1 | ||||||
Mannitol | Others | 1 | ||||||
2-O-Di-gallic acyl-glucoside | Tannins | 1 | ||||||
N-(4-O-(Glucosyl)-E-feruloyl) | Alkaloids | 1 | ||||||
(-)-Jasmonoyl-L-Isoleucine | Organic acids | 1 | ||||||
Argininosuccinic acid | Organic acids | − | − | − | 4 | |||
LysoPC 18:1(2n isomer) | Lipids | − | − | 4 | ||||
2--Linolenoyl-glycerol-1,3-di | Lipids | − | − | 4 | ||||
Dihydroxyoctadeca-dienoic acid | Lipids | − | − | 1 |
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Days | Upper Epidermis | Palisade Cell | Spongy Mesophyll | Lower Epidermis | Palisade/Spongy |
---|---|---|---|---|---|
UV-B 0 | 13.26 ± 7.58 | 35.00 ± 8.85 | 61.89 ± 2.97 | 9.15 ± 1.16 | 595.60 ± 97.67 |
UV-B 5 | 10.83 ± 0.75 | 38.62 ± 4.26 | 70.86 ± 13.34 | 8.83 ± 0.41 | 590.21 ± 127.77 |
UV-B 10 | 8.78 ± 1.02 | 32.36 ± 10.34 | 69.06 ± 5.68 | 14.03 ± 5.06 | 490.99 ± 178.91 |
UV-B 15 | 8.44 ± 0.64 | 26.33 ± 1.16 | 74.64 ± 10.15 | 8.38 ± 1.19 | 381.08 ± 55.59 * |
UV-B 20 | 7.83 ± 1.38 * | 22.83 ± 3.05 * | 70.42 ± 8.13 | 10.33 ± 6.91 | 335.46 ± 76.10 * |
Compounds | Peak Area (×104) | Log2FC | Log2FC | ||
---|---|---|---|---|---|
UV-B 0 | UV-B 10 | UV-B 20 | (UV-B 0 vs. UV-B 10) | (UV-B 0 vs. UV-B 20) | |
Kumatakenin | 1.93 ± 0.28 | 6.16 ± 2.69 | 9.40 ± 1.92 | 1.67 | 2.28 |
Icaritin | 863.34 ± 16.30 | 1871.60 ± 314.28 | 2127.57 ± 588.18 | 1.12 | 1.30 |
Wushanicaritin | 6.69 ± 0.34 | 22.36 ± 7.26 | 32.08 ± 19.82 | 1.74 | 2.26 |
Icariside I | 1347.30 ± 9.85 | 4693.27 ± 787.74 | 4084.00 ± 995.63 | 1.80 | 1.60 |
Kaempferol-3-O-glucuronide | 102.46 ± 9.53 | 222.24 ± 35.19 | 400.10 ± 136.05 | 1.12 | 1.97 |
Compounds | Peak Area (×104) | Log2FC | Log2FC | ||
---|---|---|---|---|---|
UV-B 0 | UV-B 10 | UV-B 20 | (UV-B 0 vs. UV-B 10) | (UV-B 0 vs. UV-B 20) | |
Baohuoside II | 1845.13 ± 24.92 | 293.34 ± 77.11 | 686.60 ± 227.09 | −2.65 | −1.43 |
Baohuoside III | 226.19 ± 29.43 | 24.93 ± 4.17 | 62.69 ± 19.95 | −3.18 | −1.85 |
5-O-Methylquercetin | 16.79 ± 2.82 | 5.50 ± 3.06 | 5.71 ± 1.94 | −1.61 | −1.55 |
Ikarisoside F | 556.51 ± 35.70 | 104.07 ± 28.47 | 228.32 ± 78.01 | −2.42 | −1.29 |
Isorhamnetin-3-O-rhamnoside | 147.40 ± 11.18 | 67.56 ± 25.52 | 70.18 ± 39.71 | −1.13 | −1.07 |
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Li, P.; Xiang, Q.; Wang, Y.; Dong, X. UV-B Radiation Enhances Epimedium brevicornu Maxim. Quality by Improving the Leaf Structure and Increasing the Icaritin Content. Plants 2024, 13, 1720. https://doi.org/10.3390/plants13131720
Li P, Xiang Q, Wang Y, Dong X. UV-B Radiation Enhances Epimedium brevicornu Maxim. Quality by Improving the Leaf Structure and Increasing the Icaritin Content. Plants. 2024; 13(13):1720. https://doi.org/10.3390/plants13131720
Chicago/Turabian StyleLi, Pengshu, Qiuyan Xiang, Yue Wang, and Xuehui Dong. 2024. "UV-B Radiation Enhances Epimedium brevicornu Maxim. Quality by Improving the Leaf Structure and Increasing the Icaritin Content" Plants 13, no. 13: 1720. https://doi.org/10.3390/plants13131720