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Horticulturae
Special Issues
Tissue Culture and Micropropagation Techniques of Horticultural Crops
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Tissue Culture and Micropropagation Techniques of Horticultural Crops

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Propagation and Seeds".

Deadline for manuscript submissions: 15 October 2024 | Viewed by 1961

Special Issue Editors

Dr. Tolga Izgü

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Guest Editor
National Research Council of Italy (CNR) IBE/Institute of BioEconomy Polo Scientifico, via Madonna del Piano n. 10, Sesto Fiorentino, 50019 Firenze, Italy
Interests: plant tissue culture; micropropagation; somatic embryogenesis; temporary immersion system; cryopreservation
Dr. Özhan Şimşek

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Guest Editor
Horticulture Department, Agriculture Faculty, Erciyes University, Kayseri 38030, Türkiye
Interests: plant tissue culture; micropropagation; temporary immersion system; molecular markers; gene expression
Prof. Dr. Maria Angeles Pedreño

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Guest Editor
Department of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, 30100 Murcia, Spain
Interests: plant biotechnology; antioxidant secondary metabolites; plant bioactive molecules
Special Issues, Collections and Topics in MDPI journals
Dr. Haiying Liang

E-Mail Website
Guest Editor
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Interests: improving economically important woody plant species using genomics and biotechnology plant; plant hormones

Special Issue Information

Dear Colleagues,

As the demands for enhanced efficiency, productivity, and sustainability in horticultural crop production continue to grow, we are delighted to announce our Special Issue focused on "Tissue Culture and Micropropagation Techniques of Horticultural Crops".

This dedicated issue aims to showcase cutting-edge research and advancements in various areas such as plant tissue culture, micropropagation, organogenesis, gynogenesis, androgenesis, double haploid production, somatic embryogenesis, meristem culture, embryo culture, callus culture, protoplast culture, the application of temporary immersion system (TIS), etc., within the realm of horticultural crops.

We invite contributions from researchers and experts in the field, encouraging the submission of innovative studies, methodologies, and breakthroughs that explore the transformative potential of these techniques in horticultural crop production.

Additionally, we are interested in practical and applied research, spanning from new insights into the in vitro propagation process to the conservation of horticultural crops through methods such as Slow Growth Storage (SGS) and Cryopreservation.

By bringing together a diverse range of perspectives, this Special Issue aims to offer a comprehensive overview of the latest developments in tissue culture and micropropagation. We anticipate that this collaborative effort will facilitate knowledge exchange and foster a sense of community within the horticultural research community.

We eagerly await your valuable contributions to propel the frontiers of horticultural science.

Dr. Tolga Izgü
Dr. Özhan Şimşek
Prof. Dr. Maria Angeles Pedreño
Dr. Haiying Liang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • in vitro regeneration
  • shoot culture
  • plant tissue culture
  • micropropagation
  • cryopreservation

Published Papers (2 papers)

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Research

11 pages, 807 KiB  
Article
In Vitro Micropropagation of the Vulnerable Chilean Endemic Alstroemeria pelegrina L.
by Francesca Guerra, Ricardo Cautín and Mónica Castro
Horticulturae 2024, 10(7), 674; https://doi.org/10.3390/horticulturae10070674 - 25 Jun 2024
Viewed by 383
Abstract
The Chilean rhizomatous geophyte Alstroemeria pelegrina (A. pelegrina), a species endemic to the Coquimbo (31°45′ S) and Valparaíso (33°12′ S) regions, is currently classified as vulnerable and experiencing population decline due to loss of habitat. This heightened threat underscores the need [...] Read more.
The Chilean rhizomatous geophyte Alstroemeria pelegrina (A. pelegrina), a species endemic to the Coquimbo (31°45′ S) and Valparaíso (33°12′ S) regions, is currently classified as vulnerable and experiencing population decline due to loss of habitat. This heightened threat underscores the need to develop effective mass propagation techniques to support its conservation efforts. Consequently, the present study aimed to establish an optimized in vitro propagation protocol tailored specifically for A. pelegrina to facilitate its large-scale propagation and promote the preservation of this vulnerable geophyte. In our experiment, explants obtained from in vitro germination were used. These explants were then cultured on a Murashige and Skoog (MS) medium solidified with agar (6 g L−1) and supplemented with 2.22 μM 6-Benzylaminopurine (BAP) and 30 g L−1 sucrose. The results indicated an average of 4.6 new shoots produced per initial explant. The implementation of light-emitting diode (LED) illumination with a red:blue ratio of 3:1 and 2.22 μM BAP resulted in an average shoot length of 6.2 cm. For rhizome induction, the addition of either 5.37 μM 1-naphthaleneacetic acid (NAA) or 5.37 μM indole-3-butyric acid (IBA) resulted in average rhizome lengths of 1.9 cm and 1.7 cm, respectively, with fresh weights of the vitroplants ranging from 2.9 g to 3.1 g, and rooting percentages between 100% and 96%, respectively. Notably, the survival rate of rooted plants obtained through in vitro propagation was 90% after a 4-week acclimatization period in a cold greenhouse, indicating the efficacy of the developed protocol for mass propagation and conservation efforts of this vulnerable geophyte species. Full article
(This article belongs to the Special Issue Tissue Culture and Micropropagation Techniques of Horticultural Crops)
16 pages, 4472 KiB  
Article
Assessing Cadmium Stress Resilience in Myrtle Genotypes Using Machine Learning Predictive Models: A Comparative In Vitro Analysis
by Mehmet Tütüncü, Musab A. Isak, Tolga İzgü, Dicle Dönmez, Yıldız Aka Kaçar and Özhan Şimşek
Horticulturae 2024, 10(6), 542; https://doi.org/10.3390/horticulturae10060542 - 22 May 2024
Viewed by 660
Abstract
This study investigated the effects of cadmium (Cd) stress on the micropropagation and rooting dynamics of two myrtle (Myrtus communis L.) genotypes with different fruit colors under controlled in vitro conditions. We evaluated the response of these genotypes to varying concentrations of [...] Read more.
This study investigated the effects of cadmium (Cd) stress on the micropropagation and rooting dynamics of two myrtle (Myrtus communis L.) genotypes with different fruit colors under controlled in vitro conditions. We evaluated the response of these genotypes to varying concentrations of Cd (0, 100, 200, 300, 400, and 500 µM) to determine dose-dependent effects on plantlet multiplication and root formation. Our results demonstrate that the white-fruited (WF) genotype exhibits greater resilience than the black-fruited (BF) genotype across all concentrations, maintaining higher multiplication rates and shoot heights. For instance, the multiplication rate at 100 µM Cd was highest for WF at 6.73, whereas BF showed the lowest rate of 1.94 at 500 µM. Similarly, increasing Cd levels significantly impaired root length and the number of roots for both genotypes, illustrating the detrimental impact of Cd on root system development. Additionally, this study incorporated machine learning (ML) models to predict growth outcomes. The multilayer perceptron (MLP) model, including random forest (RF) and XGBoost, was used to analyze the data. The MLP model performed notably well, demonstrating the potential of advanced computational tools in accurately predicting plant responses to environmental stress. For example, the MLP model accurately predicted shoot height with an R2 value of 0.87 and root length with an R2 of 0.99, indicating high predictive accuracy. Overall, our findings provide significant insights into the genotypic differences in Cd tolerance and the utility of ML models in plant science. These results underscore the importance of developing targeted strategies to enhance plant resilience in contaminated environments. Full article
(This article belongs to the Special Issue Tissue Culture and Micropropagation Techniques of Horticultural Crops)
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