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17 pages, 3956 KiB  
Article
EEG–fNIRS-Based Emotion Recognition Using Graph Convolution and Capsule Attention Network
by Guijun Chen, Yue Liu and Xueying Zhang
Brain Sci. 2024, 14(8), 820; https://doi.org/10.3390/brainsci14080820 - 16 Aug 2024
Viewed by 257
Abstract
Electroencephalogram (EEG) and functional near-infrared spectroscopy (fNIRS) can objectively reflect a person’s emotional state and have been widely studied in emotion recognition. However, the effective feature fusion and discriminative feature learning from EEG–fNIRS data is challenging. In order to improve the accuracy of [...] Read more.
Electroencephalogram (EEG) and functional near-infrared spectroscopy (fNIRS) can objectively reflect a person’s emotional state and have been widely studied in emotion recognition. However, the effective feature fusion and discriminative feature learning from EEG–fNIRS data is challenging. In order to improve the accuracy of emotion recognition, a graph convolution and capsule attention network model (GCN-CA-CapsNet) is proposed. Firstly, EEG–fNIRS signals are collected from 50 subjects induced by emotional video clips. And then, the features of the EEG and fNIRS are extracted; the EEG–fNIRS features are fused to generate higher-quality primary capsules by graph convolution with the Pearson correlation adjacency matrix. Finally, the capsule attention module is introduced to assign different weights to the primary capsules, and higher-quality primary capsules are selected to generate better classification capsules in the dynamic routing mechanism. We validate the efficacy of the proposed method on our emotional EEG–fNIRS dataset with an ablation study. Extensive experiments demonstrate that the proposed GCN-CA-CapsNet method achieves a more satisfactory performance against the state-of-the-art methods, and the average accuracy can increase by 3–11%. Full article
(This article belongs to the Section Cognitive Social and Affective Neuroscience)
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17 pages, 16956 KiB  
Article
Motor Fault Diagnosis Using Attention-Based Multisensor Feature Fusion
by Zhuoyao Miao, Wenshan Feng, Zhuo Long, Gongping Wu, Le Deng, Xuan Zhou and Liwei Xie
Energies 2024, 17(16), 4053; https://doi.org/10.3390/en17164053 - 15 Aug 2024
Viewed by 266
Abstract
In order to reduce the influence of environmental noise and different operating conditions on the accuracy of motor fault diagnosis, this paper proposes a capsule network method combining multi-channel signals and the efficient channel attention (ECA) mechanism, sampling the data from multiple sensors [...] Read more.
In order to reduce the influence of environmental noise and different operating conditions on the accuracy of motor fault diagnosis, this paper proposes a capsule network method combining multi-channel signals and the efficient channel attention (ECA) mechanism, sampling the data from multiple sensors and visualizing the one-dimensional time-frequency domain as a two-dimensional symmetric dot pattern (SDP) image, then fusing the multi-channel image data and extracting the image using a capsule network combining the ECA attention mechanism features to match eight different fault types for fault classification. In order to guarantee the universality of the suggested model, data from Case Western Reserve University (CWRU) is used for validation. The suggested multi-channel signal fusion ECA attention capsule network (MSF-ECA-CapsNet) model fault identification accuracy may reach 99.21%, according to the experimental findings, which is higher than the traditional method. Meanwhile, the method of multi-sensor data fusion and the use of the ECA attention mechanism make the diagnosis accuracy much higher. Full article
(This article belongs to the Section F: Electrical Engineering)
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17 pages, 2393 KiB  
Article
A Modified Bio-Inspired Optimizer with Capsule Network for Diagnosis of Alzheimer Disease
by Praveena Ganesan, G. P. Ramesh, C. Puttamdappa and Yarlagadda Anuradha
Appl. Sci. 2024, 14(15), 6798; https://doi.org/10.3390/app14156798 - 4 Aug 2024
Viewed by 437
Abstract
Recently, Alzheimer’s disease (AD) is one of the common neurodegenerative disorders, which primarily occurs in old age. Structural magnetic resonance imaging (sMRI) is an effective imaging technique used in clinical practice for determining the period of AD patients. An efficient deep learning framework [...] Read more.
Recently, Alzheimer’s disease (AD) is one of the common neurodegenerative disorders, which primarily occurs in old age. Structural magnetic resonance imaging (sMRI) is an effective imaging technique used in clinical practice for determining the period of AD patients. An efficient deep learning framework is proposed in this paper for AD detection, which is inspired from clinical practice. The proposed deep learning framework significantly enhances the performance of AD classification by requiring less processing time. Initially, in the proposed framework, the sMRI images are acquired from a real-time dataset and two online datasets including Australian Imaging, Biomarker and Lifestyle flagship work of ageing (AIBL), and Alzheimer’s Disease Neuroimaging Initiative (ADNI). Next, a fuzzy-based superpixel-clustering algorithm is introduced to segment the region of interest (RoI) in sMRI images. Then, the informative deep features are extracted in segmented RoI images by integrating the probabilistic local ternary pattern (PLTP), ResNet-50, and Visual Geometry Group (VGG)-16. Furthermore, the dimensionality reduction is accomplished by through the modified gorilla troops optimizer (MGTO). This process not only enhances the classification performance but also diminishes the processing time of the capsule network (CapsNet), which is employed to classify the classes of AD. In the MGTO algorithm, a quasi-reflection-based learning (QRBL) process is introduced for generating silverback’s quasi-refraction position for further improving the optimal position’s quality. The proposed fuzzy based superpixel-clustering algorithm and MGTO-CapsNet model obtained a pixel accuracy of 0.96, 0.94, and 0.98 and a classification accuracy of 99.88%, 96.38%, and 99.94% on the ADNI, real-time, and AIBL datasets, respectively. Full article
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19 pages, 5134 KiB  
Article
Attribute Feature Perturbation-Based Augmentation of SAR Target Data
by Rubo Jin, Jianda Cheng, Wei Wang, Huiqiang Zhang and Jun Zhang
Sensors 2024, 24(15), 5006; https://doi.org/10.3390/s24155006 - 2 Aug 2024
Viewed by 312
Abstract
Large-scale, diverse, and high-quality data are the basis and key to achieving a good generalization of target detection and recognition algorithms based on deep learning. However, the existing methods for the intelligent augmentation of synthetic aperture radar (SAR) images are confronted with several [...] Read more.
Large-scale, diverse, and high-quality data are the basis and key to achieving a good generalization of target detection and recognition algorithms based on deep learning. However, the existing methods for the intelligent augmentation of synthetic aperture radar (SAR) images are confronted with several issues, including training instability, inferior image quality, lack of physical interpretability, etc. To solve the above problems, this paper proposes a feature-level SAR target-data augmentation method. First, an enhanced capsule neural network (CapsNet) is proposed and employed for feature extraction, decoupling the attribute information of input data. Moreover, an attention mechanism-based attribute decoupling framework is used, which is beneficial for achieving a more effective representation of features. After that, the decoupled attribute feature, including amplitude, elevation angle, azimuth angle, and shape, can be perturbed to increase the diversity of features. On this basis, the augmentation of SAR target images is realized by reconstructing the perturbed features. In contrast to the augmentation methods using random noise as input, the proposed method realizes the mapping from the input of known distribution to the change in unknown distribution. This mapping method reduces the correlation distance between the input signal and the augmented data, therefore diminishing the demand for training data. In addition, we combine pixel loss and perceptual loss in the reconstruction process, which improves the quality of the augmented SAR data. The evaluation of the real and augmented images is conducted using four assessment metrics. The images generated by this method achieve a peak signal-to-noise ratio (PSNR) of 21.6845, radiometric resolution (RL) of 3.7114, and dynamic range (DR) of 24.0654. The experimental results demonstrate the superior performance of the proposed method. Full article
(This article belongs to the Section Sensing and Imaging)
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15 pages, 1537 KiB  
Article
3Cs: Unleashing Capsule Networks for Robust COVID-19 Detection Using CT Images
by Rawan Alaufi, Felwa Abukhodair and Manal Kalkatawi
COVID 2024, 4(8), 1113-1127; https://doi.org/10.3390/covid4080077 - 24 Jul 2024
Viewed by 393
Abstract
The COVID-19 pandemic has spread worldwide for over two years. It was considered a significant threat to global health due to its transmissibility and high pathogenicity. The standard test for COVID-19, namely, reverse transcription polymerase chain reaction (RT–PCR), is somehow inaccurate and might [...] Read more.
The COVID-19 pandemic has spread worldwide for over two years. It was considered a significant threat to global health due to its transmissibility and high pathogenicity. The standard test for COVID-19, namely, reverse transcription polymerase chain reaction (RT–PCR), is somehow inaccurate and might have a high false-negative rate (FNR). As a result, an infected person with a negative test result may unknowingly continue to spread the virus, especially if they are infected with an undiscovered COVID-19 strain. Thus, a more accurate diagnostic technique is required. In this study, we propose 3Cs, which is a capsule neural network (CapsNet) used to classify computed tomography (CT) images as novel coronavirus pneumonia (NCP), common pneumonia (CP), or normal lungs. Using 6123 CT images of healthy patients’ lungs and those of patients with CP and NCP, the 3Cs method achieved an accuracy of around 98% and an FNR of about 2%, demonstrating CapNet’s ability to extract features from CT images that distinguish between healthy and infected lungs. This research confirmed that using CapsNet to detect COVID-19 from CT images results in a lower FNR compared to RT–PCR. Thus, it can be used in conjunction with RT–PCR to diagnose COVID-19 regardless of the variant. Full article
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13 pages, 931 KiB  
Article
Outcome of Endoscopic Resection of Rectal Neuroendocrine Tumors ≤ 10 mm
by Roberta Elisa Rossi, Maria Terrin, Silvia Carrara, Roberta Maselli, Alexia Francesca Bertuzzi, Silvia Uccella, Andrea Gerardo Antonio Lania, Alessandro Zerbi, Cesare Hassan and Alessandro Repici
Diagnostics 2024, 14(14), 1484; https://doi.org/10.3390/diagnostics14141484 - 11 Jul 2024
Viewed by 460
Abstract
Background and aim: Guidelines suggest endoscopic resection for rectal neuroendocrine tumors (rNETs) < 10 mm, but the most appropriate resection technique is unclear. In real-life clinical practice, the endoscopic removal of unrecognized rNETs can take place with “simple” techniques and without preliminary staging. [...] Read more.
Background and aim: Guidelines suggest endoscopic resection for rectal neuroendocrine tumors (rNETs) < 10 mm, but the most appropriate resection technique is unclear. In real-life clinical practice, the endoscopic removal of unrecognized rNETs can take place with “simple” techniques and without preliminary staging. The aim of the current study is to report our own experience at a referral center for both neuroendocrine neoplasms and endoscopy. Methods: Retrospective analyses of polypectomies were performed at the Humanitas Research Hospital for rNETs (already diagnosed or previously unrecognized). Results: A total of 19 patients were included, with a median lesion size of 5 mm (range 3–10 mm). Only five lesions were suspected as NETs before removal and underwent endoscopic ultrasound (EUS) before resection, being removed with advanced endoscopic techniques. Unsuspected rNETs were removed by cold polypectomy in eleven cases, EMR in two, and biopsy forceps in one. When described, the margins were negative in four cases, positive in four (R1), and indeterminate in one. The median follow-up was 40 months. A 10 mm polypoid lesion removed with cold snare polypectomy (G2 R1) needed subsequent surgery. Eighteen patients underwent EUS after a median time of 6.5 months from resection. The EUS identified local recurrence after 14 months in a 7 mm polypoid lesion removed with cold snare polypectomy (G1 R1); the lesion was treated with cap-assisted EMR. For all the other lesions, the follow-up was negative. Conclusions: When rNETs are improperly removed without prior staging, caution must be exercised. The data from our cohort suggest that even if inappropriate resection had happened, patients may be safely managed with early EUS evaluation. Full article
(This article belongs to the Special Issue Diagnosis and Management of Neuroendocrine Tumors)
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27 pages, 4708 KiB  
Article
Using Segmentation to Boost Classification Performance and Explainability in CapsNets
by Dominik Vranay, Maroš Hliboký, László Kovács and Peter Sinčák
Mach. Learn. Knowl. Extr. 2024, 6(3), 1439-1465; https://doi.org/10.3390/make6030068 - 28 Jun 2024
Viewed by 565
Abstract
In this paper, we present Combined-CapsNet (C-CapsNet), a novel approach aimed at enhancing the performance and explainability of Capsule Neural Networks (CapsNets) in image classification tasks. Our method involves the integration of segmentation masks as reconstruction targets within the CapsNet architecture. This integration [...] Read more.
In this paper, we present Combined-CapsNet (C-CapsNet), a novel approach aimed at enhancing the performance and explainability of Capsule Neural Networks (CapsNets) in image classification tasks. Our method involves the integration of segmentation masks as reconstruction targets within the CapsNet architecture. This integration helps in better feature extraction by focusing on significant image parts while reducing the number of parameters required for accurate classification. C-CapsNet combines principles from Efficient-CapsNet and the original CapsNet, introducing several novel improvements such as the use of segmentation masks to reconstruct images and a number of tweaks to the routing algorithm, which enhance both classification accuracy and interoperability. We evaluated C-CapsNet using the Oxford-IIIT Pet and SIIM-ACR Pneumothorax datasets, achieving mean F1 scores of 93% and 67%, respectively. These results demonstrate a significant performance improvement over traditional CapsNet and CNN models. The method’s effectiveness is further highlighted by its ability to produce clear and interpretable segmentation masks, which can be used to validate the network’s focus during classification tasks. Our findings suggest that C-CapsNet not only improves the accuracy of CapsNets but also enhances their explainability, making them more suitable for real-world applications, particularly in medical imaging. Full article
(This article belongs to the Section Network)
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23 pages, 7915 KiB  
Article
Deep-Learning-Based Recovery of Missing Optical Marker Trajectories in 3D Motion Capture Systems
by Oleksandr Yuhai, Ahnryul Choi, Yubin Cho, Hyunggun Kim and Joung Hwan Mun
Bioengineering 2024, 11(6), 560; https://doi.org/10.3390/bioengineering11060560 - 1 Jun 2024
Viewed by 622
Abstract
Motion capture (MoCap) technology, essential for biomechanics and motion analysis, faces challenges from data loss due to occlusions and technical issues. Traditional recovery methods, based on inter-marker relationships or independent marker treatment, have limitations. This study introduces a novel U-net-inspired bi-directional long short-term [...] Read more.
Motion capture (MoCap) technology, essential for biomechanics and motion analysis, faces challenges from data loss due to occlusions and technical issues. Traditional recovery methods, based on inter-marker relationships or independent marker treatment, have limitations. This study introduces a novel U-net-inspired bi-directional long short-term memory (U-Bi-LSTM) autoencoder-based technique for recovering missing MoCap data across multi-camera setups. Leveraging multi-camera and triangulated 3D data, this method employs a sophisticated U-shaped deep learning structure with an adaptive Huber regression layer, enhancing outlier robustness and minimizing reconstruction errors, proving particularly beneficial for long-term data loss scenarios. Our approach surpasses traditional piecewise cubic spline and state-of-the-art sparse low rank methods, demonstrating statistically significant improvements in reconstruction error across various gap lengths and numbers. This research not only advances the technical capabilities of MoCap systems but also enriches the analytical tools available for biomechanical research, offering new possibilities for enhancing athletic performance, optimizing rehabilitation protocols, and developing personalized treatment plans based on precise biomechanical data. Full article
(This article belongs to the Special Issue Biomechanics and Motion Analysis)
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19 pages, 3496 KiB  
Article
Capsule Broad Learning System Network for Robust Synthetic Aperture Radar Automatic Target Recognition with Small Samples
by Cuilin Yu, Yikui Zhai, Haifeng Huang, Qingsong Wang and Wenlve Zhou
Remote Sens. 2024, 16(9), 1526; https://doi.org/10.3390/rs16091526 - 26 Apr 2024
Viewed by 710
Abstract
The utilization of deep learning in Synthetic Aperture Radar (SAR) Automatic Target Recognition (ATR) has witnessed a recent surge owing to its remarkable feature extraction capabilities. Nonetheless, deep learning methodologies are often encumbered by inadequacies in labeled data and the protracted nature of [...] Read more.
The utilization of deep learning in Synthetic Aperture Radar (SAR) Automatic Target Recognition (ATR) has witnessed a recent surge owing to its remarkable feature extraction capabilities. Nonetheless, deep learning methodologies are often encumbered by inadequacies in labeled data and the protracted nature of training processes. To address these challenges and offer an alternative avenue for accurately extracting image features, this paper puts forth a novel and distinctive network dubbed the Capsule Broad Learning System Network for robust SAR ATR (CBLS-SARNET). This novel strategy is specifically tailored to cater to small-sample SAR ATR scenarios. On the one hand, we introduce a United Division Co-training (UDC) Framework as a feature filter, adeptly amalgamating CapsNet and the Broad Learning System (BLS) to enhance network efficiency and efficacy. On the other hand, we devise a Parameters Sharing (PS) network to facilitate secondary learning by sharing the weight and bias of BLS node layers, thereby augmenting the recognition capability of CBLS-SARNET. Experimental results unequivocally demonstrate that our proposed CBLS-SARNET outperforms other deep learning methods in terms of recognition accuracy and training time. Furthermore, experiments validate the generalization and robustness of our novel method under various conditions, including the addition of blur, Gaussian noise, noisy labels, and different depression angles. These findings underscore the superior generalization capabilities of CBLS-SARNET across diverse SAR ATR scenarios. Full article
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21 pages, 4421 KiB  
Article
Research on a Capsule Network Text Classification Method with a Self-Attention Mechanism
by Xiaodong Yu, Shun-Nain Luo, Yujia Wu, Zhufei Cai, Ta-Wen Kuan and Shih-Pang Tseng
Symmetry 2024, 16(5), 517; https://doi.org/10.3390/sym16050517 - 24 Apr 2024
Viewed by 734
Abstract
Convolutional neural networks (CNNs) need to replicate feature detectors when modeling spatial information, which reduces their efficiency. The number of replicated feature detectors or labeled training data required for such methods grows exponentially with the dimensionality of the data being used. On the [...] Read more.
Convolutional neural networks (CNNs) need to replicate feature detectors when modeling spatial information, which reduces their efficiency. The number of replicated feature detectors or labeled training data required for such methods grows exponentially with the dimensionality of the data being used. On the other hand, space-insensitive methods are difficult to encode and express effectively due to the limitation of their rich text structures. In response to the above problems, this paper proposes a capsule network (self-attention capsule network, or SA-CapsNet) with a self-attention mechanism for text classification tasks, wherein the capsule network itself, given the feature with the symmetry hint on two ends, acts as both encoder and decoder. In order to learn long-distance dependent features in sentences and encode text information more efficiently, SA-CapsNet maps the self-attention module to the feature extraction layer of the capsule network, thereby increasing its feature extraction ability and overcoming the limitations of convolutional neural networks. In addition, in this study, in order to improve the accuracy of the model, the capsule was improved by reducing its dimension and an intermediate layer was added, enabling the model to obtain more expressive instantiation features in a given sentence. Finally, experiments were carried out on three general datasets of different sizes, namely the IMDB, MPQA, and MR datasets. The accuracy of the model on these three datasets was 84.72%, 80.31%, and 75.38%, respectively. Furthermore, compared with the benchmark algorithm, the model’s performance on these datasets was promising, with an increase in accuracy of 1.08%, 0.39%, and 1.43%, respectively. This study focused on reducing the parameters of the model for various applications, such as edge and mobile applications. The experimental results show that the accuracy is still not apparently decreased by the reduced parameters. The experimental results therefore verify the effective performance of the proposed SA-CapsNet model. Full article
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19 pages, 6233 KiB  
Article
Fault Diagnosis for Power Batteries Based on a Stacked Sparse Autoencoder and a Convolutional Block Attention Capsule Network
by Juan Zhou, Shun Zhang and Peng Wang
Processes 2024, 12(4), 816; https://doi.org/10.3390/pr12040816 - 18 Apr 2024
Viewed by 856
Abstract
The power battery constitutes the fundamental component of new energy vehicles. Rapid and accurate fault diagnosis of power batteries can effectively improve the safety and power performance of the vehicle. In response to the issues of limited generalization ability and suboptimal diagnostic accuracy [...] Read more.
The power battery constitutes the fundamental component of new energy vehicles. Rapid and accurate fault diagnosis of power batteries can effectively improve the safety and power performance of the vehicle. In response to the issues of limited generalization ability and suboptimal diagnostic accuracy observed in traditional power battery fault diagnosis models, this study proposes a fault diagnosis method utilizing a Convolutional Block Attention Capsule Network (CBAM-CapsNet) based on a stacked sparse autoencoder (SSAE). The reconstructed dataset is initially input into the SSAE model. Layer-by-layer greedy learning using unsupervised learning is employed, combining unsupervised learning methods with parameter updating and local fine-tuning to enhance visualization capabilities. The CBAM is then integrated into the CapsNet, which not only mitigates the effect of noise on the SSAE but also improves the model’s ability to characterize power cell features, completing the fault diagnosis process. The experimental comparison results show that the proposed method can diagnose power battery failure modes with an accuracy of 96.86%, and various evaluation indexes are superior to CNN, CapsNet, CBAM-CapsNet, and other neural networks at accurately identifying fault types with higher diagnostic accuracy and robustness. Full article
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20 pages, 27165 KiB  
Article
MES-CTNet: A Novel Capsule Transformer Network Base on a Multi-Domain Feature Map for Electroencephalogram-Based Emotion Recognition
by Yuxiao Du, Han Ding, Min Wu, Feng Chen and Ziman Cai
Brain Sci. 2024, 14(4), 344; https://doi.org/10.3390/brainsci14040344 - 30 Mar 2024
Cited by 1 | Viewed by 1028
Abstract
Emotion recognition using the electroencephalogram (EEG) has garnered significant attention within the realm of human–computer interaction due to the wealth of genuine emotional data stored in EEG signals. However, traditional emotion recognition methods are deficient in mining the connection between multi-domain features and [...] Read more.
Emotion recognition using the electroencephalogram (EEG) has garnered significant attention within the realm of human–computer interaction due to the wealth of genuine emotional data stored in EEG signals. However, traditional emotion recognition methods are deficient in mining the connection between multi-domain features and fitting their advantages. In this paper, we propose a novel capsule Transformer network based on a multi-domain feature for EEG-based emotion recognition, referred to as MES-CTNet. The model’s core consists of a multichannel capsule neural network(CapsNet) embedded with ECA (Efficient Channel Attention) and SE (Squeeze and Excitation) blocks and a Transformer-based temporal coding layer. Firstly, a multi-domain feature map is constructed by combining the space–frequency–time characteristics of the multi-domain features as inputs to the model. Then, the local emotion features are extracted from the multi-domain feature maps by the improved CapsNet. Finally, the Transformer-based temporal coding layer is utilized to globally perceive the emotion feature information of the continuous time slices to obtain a final emotion state. The paper fully experimented on two standard datasets with different emotion labels, the DEAP and SEED datasets. On the DEAP dataset, MES-CTNet achieved an average accuracy of 98.31% in the valence dimension and 98.28% in the arousal dimension; it achieved 94.91% for the cross-session task on the SEED dataset, demonstrating superior performance compared to traditional EEG emotion recognition methods. The MES-CTNet method, utilizing a multi-domain feature map as proposed herein, offers a broader observation perspective for EEG-based emotion recognition. It significantly enhances the classification recognition rate, thereby holding considerable theoretical and practical value in the EEG emotion recognition domain. Full article
(This article belongs to the Section Computational Neuroscience and Neuroinformatics)
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22 pages, 3228 KiB  
Article
Production Decision Model for the Cement Industry in Pursuit of Carbon Neutrality: Analysis of the Impact of Carbon Tax and Carbon Credit Costs
by Wen-Hsien Tsai and Wei-Hong Lin
Sustainability 2024, 16(6), 2251; https://doi.org/10.3390/su16062251 - 7 Mar 2024
Viewed by 1034
Abstract
One of the solutions to achieve the goal of net-zero emissions by 2050 is to try to reduce the carbon emission by using the carbon tax or carbon credit (carbon right). This paper examines the impact of carbon taxes and carbon credit costs [...] Read more.
One of the solutions to achieve the goal of net-zero emissions by 2050 is to try to reduce the carbon emission by using the carbon tax or carbon credit (carbon right). This paper examines the impact of carbon taxes and carbon credit costs on the cement industry, focusing on ESG indicators and corporate profits. Utilizing Activity-Based Costing and the Theory of Constraints, a production decision model is developed and analyzed using mathematical programming. The paper categorizes carbon tax models into continuous and discontinuous progressive tax rates, taking into account potential government policies like emission tax exemptions and carbon trading. It finds that reducing emission caps is more effective than increasing carbon tax rates in curbing emissions. These insights can assist governments in policy formulation and provide a reference framework for establishing carbon tax systems. Full article
(This article belongs to the Topic Multiple Roads to Achieve Net-Zero Emissions by 2050)
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21 pages, 7892 KiB  
Article
A Study of Hyaluronic Acid’s Theoretical Reactivity and of Magnetic Nanoparticles Capped with Hyaluronic Acid
by Mihaela Răcuciu, Simona Oancea, Lucian Barbu-Tudoran, Olga Drăghici, Anda Agavriloaei and Dorina Creangă
Materials 2024, 17(6), 1229; https://doi.org/10.3390/ma17061229 - 7 Mar 2024
Viewed by 1271
Abstract
Hyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed by [...] Read more.
Hyaluronic acid (HA) has attracted much attention in tumor-targeted drug delivery due to its ability to specifically bind to the CD44 cellular receptor, which is widely expressed on cancer cells. We present HA-capped magnetic nanoparticles (HA-MNPs) obtained via the co-precipitation method, followed by the electrostatic adsorption of HA onto the nanoparticles’ surfaces. A theoretical study carried out with the PM3 method evidenced a dipole moment of 3.34 D and negatively charged atom groups able to participate in interactions with nanoparticle surface cations and surrounding water molecules. The ATR-FTIR spectrum evidenced the hyaluronic acid binding to the surface of the ferrophase, ensuring colloidal stability in the water dispersion. To verify the success of the synthesis and stabilization, HA-MNPs were also characterized using other investigation techniques: TEM, EDS, XRD, DSC, TG, NTA, and VSM. The results showed that the HA-MNPs had a mean physical size of 9.05 nm (TEM investigation), a crystallite dimension of about 8.35 nm (XRD investigation), and a magnetic core diameter of about 8.31 nm (VSM investigation). The HA-MNPs exhibited superparamagnetic behavior, with the magnetization curve showing saturation at a high magnetic field and a very small coercive field, corresponding to the net dominance of single-domain magnetic nanoparticles that were not aggregated with reversible magnetizability. These features satisfy the requirement for magnetic nanoparticles with a small size and good dispersibility for long-term stability. We performed some preliminary tests regarding the nanotoxicity in the environment, and some chromosomal aberrations were found to be induced in corn root meristems, especially in the anaphase and metaphase of mitotic cells. Due to their properties, HA-MNPs also seem to be suitable for use in the biomedical field. Full article
(This article belongs to the Section Advanced Nanomaterials and Nanotechnology)
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28 pages, 684 KiB  
Review
Neutrophil Extracellular DNA Traps in Response to Infection or Inflammation, and the Roles of Platelet Interactions
by William A. Chen and Danilo S. Boskovic
Int. J. Mol. Sci. 2024, 25(5), 3025; https://doi.org/10.3390/ijms25053025 - 5 Mar 2024
Cited by 2 | Viewed by 1551
Abstract
Neutrophils present the host’s first line of defense against bacterial infections. These immune effector cells are mobilized rapidly to destroy invading pathogens by (a) reactive oxygen species (ROS)-mediated oxidative bursts and (b) via phagocytosis. In addition, their antimicrobial service is capped via a [...] Read more.
Neutrophils present the host’s first line of defense against bacterial infections. These immune effector cells are mobilized rapidly to destroy invading pathogens by (a) reactive oxygen species (ROS)-mediated oxidative bursts and (b) via phagocytosis. In addition, their antimicrobial service is capped via a distinct cell death mechanism, by the release of their own decondensed nuclear DNA, supplemented with a variety of embedded proteins and enzymes. The extracellular DNA meshwork ensnares the pathogenic bacteria and neutralizes them. Such neutrophil extracellular DNA traps (NETs) have the potential to trigger a hemostatic response to pathogenic infections. The web-like chromatin serves as a prothrombotic scaffold for platelet adhesion and activation. What is less obvious is that platelets can also be involved during the initial release of NETs, forming heterotypic interactions with neutrophils and facilitating their responses to pathogens. Together, the platelet and neutrophil responses can effectively localize an infection until it is cleared. However, not all microbial infections are easily cleared. Certain pathogenic organisms may trigger dysregulated platelet–neutrophil interactions, with a potential to subsequently propagate thromboinflammatory processes. These may also include the release of some NETs. Therefore, in order to make rational intervention easier, further elucidation of platelet, neutrophil, and pathogen interactions is still needed. Full article
(This article belongs to the Special Issue Neutrophil in Cell Biology and Diseases 2.0)
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