Search Results (4,338)

Secondary flowering is the phenomenon in which a tree blooms twice or more times a year. Along with the development of blueberry (Vaccinium corymbosum L.) fruits in spring, a large number of secondary flowers on the strong upright spring shoots were noticed in blueberries planted in the greenhouse. To reveal the cause and possible regulatory mechanism of the phenomenon, we clarified the phenological characteristics of flower bud differentiation and development on the spring shoots by combining phenological phenotype with anatomical observation. Furthermore, the changes in carbohydrates, trehalose-6-phosphate (Tre6P), and the relationship among the key enzyme regulatory genes for Tre6P metabolism and the key regulatory genes for flower formation during the differentiation process of apical buds and axillary buds were investigated. The results showed that the process of flower bud differentiation and flowering of apical and axillary buds was consistent, accompanied by a large amount of carbohydrate consumption. This process was positively correlated with the expression trends of VcTPS1/2, VcSnRK1, VcFT, VcLFY2, VcSPL43, VcAP1, and VcDAM in general, and negatively correlated with that of VcTPP. In addition, there is a certain difference in the differentiation progress of flower buds between the apical and axillary buds. Compared with axillary buds, apical buds had higher contents of sucrose, fructose, glucose, Tre6P, and higher expression levels of VcTPS2, VcFT, VcSPL43, and VcAP1. Moreover, VcTPS1 and VcTPS2 were more closely related to the physiological substances (sucrose and Tre6P) in axillary bud and apical bud differentiation, respectively. It was suggested that sucrose and trehalose-6-phosphate play a crucial role in promoting flower bud differentiation in strong upright spring shoots, and VcTPS1 and VcTPS2 might play a central role in these activities. Our study provided substantial sight for further study on the mechanism of multiple flowering of blueberries and laid a foundation for the regulation and utilization of the phenomenon of multiple flowering in a growing season of perennial woody plants. Full article

This experimental study focused on the measurement and analysis of the impact force caused by a tsunami bore on a coastal structure. The bore wave was produced by a dam break mechanism. The water depth in the reservoir and the location of the coastal structures were varied to simulate different impact scenarios. The time history of the force resulting from the impact of the bore wave on the coastal structure was measured. The propagation of the bore wave along the flume was recorded and the video recordings were converted into digital data using an image-processing technique in order to determine the flow depth variations with time. The hydrostatic forces and the corresponding depth and time-averaged hydrodynamic forces as well as the maximum hydrodynamic forces were acquired for each scenario. The ratio of hydrodynamic to hydrostatic forces were obtained, and it was observed that the calculated averaged ratio was within the recommended design ratio. The results indicate that an increase in the reservoir level caused an increase in the magnitude and intensity of the impact forces, however, the relationship was non-linear. Moreover, it was found that the location of the structure did not play a significant role on the intensity of the impact forces. Full article

The objective of this study is to analyze the temporal variability in water levels of Lake Mégantic (27.4 km²) during the period 1920–2020 in relation to anthropogenic and natural factors on the one hand, and its impact on the intensity and frequency of heavy flooding (recurring floods ≥ 10 years) of the Chaudière River of which it is the source, on the other hand. The application of four different Mann–Kendall tests showed a significant decrease in lake water levels during this period. The Lombard test revealed two breaks in the average daily maximum and average water levels, but only one break in the average daily minimum water levels. The first shift, which was smoothed, occurred between 1957 and 1963. It was caused by the demolition in 1956 of the first dam built in 1893 and the significant storage of water in the dams built upstream of the lake between 1956 and 1975. The second shift, which was rather abrupt, occurred between 1990 and 1993. It was caused by the voluntary and controlled lowering of the lake’s water levels in 1993 to increase the surface area of the beaches for recreational purposes. However, despite this influence of anthropogenic factors on this drop in water levels, they are negatively correlated with the global warming climate index. It is therefore a covariation, due to anthropogenic factors whose impacts are exerted at different spatial scales, without a physical causal link. However, the winter daily minimum water levels, whose temporal variability has not been influenced by anthropogenic activities, are positively correlated with the NAO and AO indices, but negatively with PDO. Finally, since the transformation of Lake Mégantic into a reservoir following the construction of the Mégantic dam in 1893 and 1973 to control heavy flooding in the Chaudière River, all recurrent floods ≥ 10 years have completely disappeared in the section of this river located downstream of Lake Mégantic. However, the disappearance of these floods and the drop in water levels of Lake Mégantic have not significantly impacted the stationarity in the flow series of the Chaudière River since 1920. Full article

Tunnel disease detection and maintenance are critical tasks in urban engineering, and are essential for the safety and stability of urban transportation systems. Water stain detection presents unique challenges due to its variable morphology and scale, which leads to insufficient multiscale contextual information extraction and boundary information loss in complex environments. To address these challenges, this paper proposes a method called Deep Aggregation Network with Edge Information Supplement (DAEiS-Net) for detecting tunnel water stains. The proposed method employs a classic encoder–decoder architecture. Specifically, in the encoder part, a Deep Aggregation Module (DAM) is introduced to enhance feature representation capabilities. Additionally, a Multiscale Cross-Attention Module (MCAM) is proposed to suppress noise in the shallow features and enhance the texture information of the high-level features. Moreover, an Edge Information Supplement Module (EISM) is designed to mitigate semantic gaps across different stages of feature extraction, improving the extraction of water stain edge information. Furthermore, a Sub-Pixel Module (SPM) is proposed to fuse features at various scales, enhancing edge feature representation. Finally, we introduce the Tunnel Water Stain Dataset (TWS), specifically designed for tunnel water stain segmentation. Experimental results on the TWS dataset demonstrate that DAEiS-Net achieves state-of-the-art performance in tunnel water stain segmentation. Full article

Sugarcane smut, caused by the fungus Sporisorium scitamineum (Sydow), significantly affects sugarcane crops worldwide. Infected plants develop whip-like structures known as sori. Significant variations in these whip lengths are commonly observed, but the physiological and molecular differences causing these morphological differences remain poorly documented. To address this, we employed conventional microbe isolation, metagenomic, and metabolomic techniques to investigate smut-infected sugarcane stems and whips of varying lengths. Metagenomics analysis revealed a diverse fungal community in the sugarcane whips, with Sporisorium and Fusarium genera notably present (>1%) in long whips. Isolation techniques confirmed these findings. Ultra-performance liquid chromatography analysis (UHPLC-MS/MS) showed high levels of gibberellin hormones (GA₃, GA₁, GA₄, GA₈, and GA₇) in long whips, with GA₄ and GA₇ found exclusively in long whips and stems. Among the prominent genera present within long whips, Fusarium was solely positively correlated with these gibberellin (GA) hormones, with the exception of GA₈, which was positively correlated with Sporisorium. KEGG enrichment analysis linked these hormones to pathways like diterpenoid biosynthesis and plant hormone signal transduction. These findings suggest that Fusarium may influence GA production leading to whip elongation. Our study reveals fungal dynamics and gibberellin responses in sugarcane smut whips. Future research will explore the related molecular gibberellin synthesis mechanisms. Full article

Semen cryopreservation results in the differential remodeling of the molecules presented in sperm, and these alterations related to reductions in sperm quality and its physiological function have not been fully understood. Given this, this study aimed to investigate the cryoinjury mechanism of goat sperm by analyzing changes of the metabolic characteristics in sperm during the cryopreservation process. The ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) technique was performed to explore metabolite profiles of fresh sperm (C group), equilibrated sperm (E group), and frozen–thawed sperm (F group). In total, 2570 metabolites in positive mode and 2306 metabolites in negative mode were identified, respectively. After comparative analyses among these three groups, 374 differentially abundant metabolites (DAMs) in C vs. E, 291 DAMs in C vs. F, and 189 DAMs in E vs. F were obtained in the positive mode; concurrently, 530 DAMs in C vs. E, 405 DAMs in C vs. F, and 193 DAMs in E vs. F were obtained in the negative mode, respectively. The DAMs were significantly enriched in various metabolic pathways, including 31 pathways in C vs. E, 25 pathways in C vs. F, and 28 pathways in E vs. F, respectively. Among them, 65 DAMs and 25 significantly enriched pathways across the three comparisons were discovered, which may be tightly associated with sperm characteristics and function. Particularly, the functional terms such as TCA cycle, biosynthesis of unsaturated fatty acids, sphingolipid metabolism, glycine, serine and threonine metabolism, alpha-linolenic acid metabolism, and pyruvate metabolism, as well as associated pivotal metabolites like ceramide, betaine, choline, fumaric acid, L-malic acid and L-lactic acid, were focused on. In conclusion, our research characterizes the composition of metabolites in goat sperm and their alterations induced by the cryopreservation process, offering a critical foundation for further exploring the molecular mechanisms of metabolism influencing the quality and freezing tolerance of goat sperm. Additionally, the impacts of equilibration at low temperature on sperm quality may need more attentions as compared to the freezing and thawing process. Full article

Both maternal obesity and postnatal consumption of obesogenic diets contribute to the development of metabolic dysfunction-associated steatotic liver disease (MASLD) and hepatocellular carcinoma (HCC). However, there is no consensus as to whether diets that are high in fat or carbohydrates/sugars differentially influence the development of HCC. Moreover, the long-term effects of prenatal HF exposure on HCC and whether this is influenced by postnatal diet has not yet been evaluated. C57BL/6 dams were fed either a low-fat, high-carbohydrate control (C) or low-carbohydrate, high-fat (HF) diet. At weaning, male and female offspring were fed the C or HF diet, generating four diet groups: C/C, C/HF, HF/C and HF/HF. Tissues were collected at 16 months of age and livers were assessed for MASLD and HCC. Glucose regulation and pancreatic morphology were also evaluated. Liver tissues were assessed for markers of glycolysis and fatty acid metabolism and validated using a human HCC bioinformatic database. Both C/HF and HF/HF mice developed obesity, hyperinsulinemia and a greater degree of MASLD than C/C and HF/C offspring. However, despite significant liver and pancreas pathology, C/HF mice had the lowest incidence of HCC while tumour burden was highest in HF/C male offspring. The molecular profile of HCC mouse samples suggested an upregulation of the pentose phosphate pathway and a downregulation of fatty acid synthesis and oxidation, which was largely validated in the human dataset. Both pre-weaning HF diet exposure and post-weaning consumption of a high-carbohydrate diet increased the risk of developing spontaneous HCC in aged mice. However, the influence of pre-weaning HF feeding on HCC development appeared to be stronger in the context of post-weaning obesity. As rates of maternal obesity continue to rise, this has implications for the future incidence of HCC and possible dietary manipulation of offspring carbohydrate intake to counteract this risk. Full article

Investigating fish diversity in canyon rivers through conventional fish surveys is challenging due to precipitous conditions, including steep slopes, rapid water flow, and complex habitats. Additionally, intensive construction of dams has further complicated the understanding of contemporary fish diversity in these rivers. In this study, we used the environmental DNA (eDNA) technique to assess fish diversity and examine the effects of dams on fish diversity in the Mabiehe River, a canyon river in the upper reaches of the Pearl River drainage. Water samples from 15 sampling sites were collected, yielding 9,356,148 valid sequences. Utilizing the NCBI public database, a total of 60 freshwater fish species were identified, with Carassius auratus, Cyprinus carpio, and Pelteobagrus fulvidraco being the most dominant species in the Mabiehe River. We also detected one nationally protected fish species, three provincially protected fish species, and six exotic species in this river. Furthermore, eDNA analyses demonstrated that the lotic river sections harbor more species and greater diversity than dammed sections, suggesting that dams might exert significant impacts on local fish diversity. Overall, this study supports the effectiveness of the eDNA technique as a complementary tool to traditional field surveys for monitoring fish biodiversity in canyon rivers. Full article

The research investigated how women employ various adaptation and coping mechanisms to alleviate food insecurity resulting from the impacts of climate change. The documentation of the debate on the role of women in adaptation and coping with climate change is relatively limited. Climate change’s effect on food security in semi-arid areas could potentially increase the population of individuals residing in severe poverty. Over the past three decades, Africa’s sub-tropics have experienced irregular rainfall and prolonged droughts, which have negatively affected agriculture and food production. This research utilized a combination of qualitative and quantitative approaches within a mixed-method design, guided by the pragmatic paradigm. Based on the results of the study, water harvesting/dam construction and income generating projects (IGPs) were identified as the most effective coping strategies for women. This study recommends implementing awareness campaigns to educate women farmers about the negative effects of climate change and the need for integrated and comprehensive capacity-building frameworks. By understanding the challenges women face in adapting to and coping with climate change, it is hoped that more effective and sustainable solutions can be developed. Full article

Neonicotinoids, a neuro-effective class of insecticides, are heavily applied in agricultural activities worldwide. Poultry can be exposed to neonicotinoids by several routes, but the knowledge of neonicotinoid’s metabolism in poultry and its associated interspecies differences is highly limited. Hence, this study aims to investigate the species differences in metabolite formations, as well as cytochrome P450 (CYP)-dependent metabolism of four major neonicotinoid compounds, acetamiprid, imidacloprid, clothianidin, and thiamethoxam, in poultry. In vitro biotransformation assays using hepatic microsomes of chicken, ducks, geese, quails, and rats were conducted. Metabolites of neonicotinoids were then screened by LC/Q-TOF and quantified by LC/MS/MS. The results revealed an existence of interspecies differences in the formations of N-[(6-chloro-3-pyridyl) methyl] -N-methyl acetamidine (IM-1-5) of acetamiprid and dm-clothianidin of clothianidin between chicken and other species. In addition, the greatest CYP activities in the metabolism of most neonicotinoid substrates, such as acetamiprid to dm-acetamiprid, imidacloprid to hydroxylated-imidacloprid and imidacloprid-olefin, clothianidin to dm-clothianidin, and thiamethoxam to clothianidin, were found in chicken. These results suggested that the CYPs in chicken may have a greater capacity for metabolism of neonicotinoids compared to other poultry. This study further revealed that the maximum intrinsic clearance of dn-imidacloprid and dn-clothianidin in ducks may be superintended by CYP-mediated nitro-reductions of imidacloprid and clothianidin. Further studies employing CYP recombinant enzymes may be required to elucidate the specific CYP isoforms that may be involved in neonicotinoid metabolism in avian species. Full article

Many dammed reservoirs in dry climate conditions witness high sediment inflow rates due to higher soil erodibility, yet there are limited actual sediment influx measurements. Therefore, this study first applies the Soil and Water Assessment Tool (SWAT) hydrologic model to simulate reservoir sedimentation inflow to the Haditha Reservoir. Next, utilizing sediment inflows estimated by the SWAT model, the Trap Efficiency Function (TEF) is employed to estimate its remaining storage capacity and its useful life at multiple reservoir water levels. Calibration (1986–1997) and validation (1998–2007) of the SWAT model were conducted at three streamflow gaging stations and one sediment station located upstream of the reservoir. Results show that the SWAT model performed better during calibration than during the validation period for all streamflow and sediment gaging stations. In addition, modeled streamflow and sediment predictions were relatively more accurate on a monthly scale than on a daily scale. Simulated daily sediment inflow to the reservoir demonstrates slightly lower accuracy than daily streamflow, where the Coefficient of Determination (R²) and Nash-Sutcliffe Efficiency values are 0.34 and 0.32 in the case of sediment load, compared to 0.39 and 0.33 for streamflow, respectively. Reservoir storage capacity for the period (1986–2005) shows a continuous decrease with time at all reservoir water levels, which indicates an increase in sediment accumulation. According to measurements taken between 1986 and 2005, sediment accumulation has reduced the reservoir’s capacity by approximately 15% at a water level of 112 m (the lowest water level in the reservoir). During the same period, the storage capacity loss at 147 m (the design working water level in the reservoir) was calculated to be 35%. Over 19 years of operation (1986–2005) at the 147-m water level, the total sediment buildup in the reservoir is estimated at 3.2 million tons. Notably, about one-third of this sediment was deposited in the five-year span from 2000 to 2005. Full article

The microbial biomass in soil is an active and living constituent of organic matter. It is both a storage pool and a source of plant nutrients that can be used as required. In addition, each microbial indicator evaluates soil quality and health from different perspectives, which are not necessarily very different. This study was conducted to compare some physical, chemical, and biochemical characteristics of the soils of forest (S_F) and deforested (S_DE) areas located on the slopes of the Kirazlıköprü area, which was previously deforested due to dam construction in Bartın province in northwestern Turkey. Soil samples were taken from the topsoil surface (0–5 cm) to determine the microbial soil characteristics of the S_F and S_DE sites. The soil microbial biomass N (N_mic) was determined by chloroform fumigation extraction, and the C_mic/N_mic ratio and N_mic/N_total percentage were calculated using the original values. Total N, N_mic and C_mic/N_mic values are higher in the forest area. The lowest and highest total N (N_total) contents in the S_F and S_DE soils varied between 1.50 and 3.47 g kg⁻¹ and 0.91 and 1.46 g kg⁻¹, respectively. Similarly, the N_mic contents of the S_F and S_DE soils varied between 75.56 and 143.42 μg g⁻¹ and 10.40 and 75.96 μg g⁻¹, respectively. A statistical analysis revealed that the mean N_total and mean N_mic values differed (p < 0.05) in the S_F and S_DE soils. The mean C_mic/N_mic values in the S_F and S_DE soils were 8.79 (±1.65) and 5.64 (±1.09), respectively, and a statistical difference was found between the fields (p < 0.05). Our findings indicate that the soil microbial community structure varies according to the site. As a result, it can be concluded that deforestation and erosion due to dam construction in the area led to the removal of plant nutrients from the soil; deterioration in the amount and activity of microbial biomass; and, consequently, soil losses and degradation of soil quality. Full article

Numerous studies indicate that intrauterine growth restriction (IUGR) can predispose individuals to metabolic syndrome (MetS) in adulthood. Several reports have demonstrated that pharmacological concentrations of biotin have therapeutic effects on MetS. The present study investigated the beneficial effects of prenatal biotin supplementation in a rat model of intrauterine caloric restriction to prevent cardiometabolic risk in adult female offspring fed fructose after weaning. Female rats were exposed to a control (C) diet or global caloric restriction (20%) (GCR), with biotin (GCRB) supplementation (2 mg/kg) during pregnancy. Female offspring were exposed to 20% fructose (F) in drinking water for 16 weeks after weaning (C, C/F, GCR/F, and GCRB/F). The study assessed various metabolic parameters including Lee’s index, body weight, feed conversion ratio, caloric intake, glucose tolerance, insulin resistance, lipid profile, hepatic triglycerides, blood pressure, and arterial vasoconstriction. Results showed that GCR and GCRB dams had reduced weights compared to C dams. Offspring of GCRB/F and GCR/F dams had lower body weight and Lee’s index than C/F offspring. Maternal biotin supplementation in the GCRB/F group significantly mitigated the adverse effects of fructose intake, including hypertriglyceridemia, hypercholesterolemia, hepatic steatosis, glucose and insulin resistance, hypertension, and arterial hyperresponsiveness. This study concludes that prenatal biotin supplementation can protect against cardiometabolic risk in adult female offspring exposed to postnatal fructose, highlighting its potential therapeutic benefits. Full article

Fishways act as ecological corridors, enabling migratory fish species to surmount barriers such as weirs or dams, which are crucial for the restoration of river ecosystems. The island-type fishway is a novel design that utilizes a combination of island structures and valvular configurations to dissipate the kinetic energy of water flow, decelerate the water velocity, and thus reduce the challenge faced by fish attempting to ascend the watercourse. The impact of valvular configurations on the hydrodynamic characteristics within an island-type fishway was explored. The results showed that the main high-velocity flow exhibits a nearly “S”-shaped characteristic, while a low-velocity region develops downstream of the valvular. The valvular configuration has a significant effect on the internal flow dynamics of the island-type fishway. Specifically, the smaller the valvular arc angle, the broader the high-velocity main flow becomes, and the smaller the area of the low-velocity region. When the valvular arc angle is set at 180°, the area dominated by low flow velocities maintains a coverage of over 60%. As the valvular arc angle decreases, turbulent kinetic energy rises, leading to an approximate 70% increase in the maximum turbulent kinetic energy across different water layers relative to the model with the initial angle setting. Within the range of valvular arc angles studied, an island-type fishway with a 180° valvular arc angle is most conducive to supporting the upstream migration of fish. This study can provide a reference for the further development of island-type fishways. Full article

Governments around the world promote the purchase of electric vehicles (EVs) as part of their climate change strategy, with many committing to EV-only sales of new passenger vehicles by 2035 and complete use of EVs by 2045 (California) or 2050 (e.g., Canada, EU). Rebates (purchase subsidies) are offered to consumers to promote uptake of EVs, and growth in their uptake has been quite strong, although EVs remain a small proportion of registered vehicles. In this study, we first analyze the economics of EV subsidies and then use Canadian electrical generation capacity, EV efficiency data, and distances driven, along with Monte Carlo simulation, to project the increased demands that greater numbers of EVs will place on an electrical grid. We find that the current grid’s capacity will not be adequate to power the anticipated growth in EVs, and major new power plants or hydroelectric dams will need to be constructed. The analysis suggests that Canada might need to build 17 new hydroelectric facilities or 14 additional gas plants, as there is likely to be much resistance to new hydroelectric projects. Full article