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17 pages, 3000 KiB  
Article
Characteristics of PM2.5 and CO2 Concentrations in Typical Functional Areas of a University Campus in Beijing Based on Low-Cost Sensor Monitoring
by Qingqing Wang, Ruoxi Ao, Hongwei Chen, Jialin Li, Lianfang Wei and Zifa Wang
Atmosphere 2024, 15(9), 1044; https://doi.org/10.3390/atmos15091044 (registering DOI) - 29 Aug 2024
Abstract
The air quality in educational campuses affects the health and work efficiency of teachers and students. Studies into this matter are of great significance for optimizing the management of campus living environments. Low-cost online sensors to monitor PM2.5 and CO2 levels [...] Read more.
The air quality in educational campuses affects the health and work efficiency of teachers and students. Studies into this matter are of great significance for optimizing the management of campus living environments. Low-cost online sensors to monitor PM2.5 and CO2 levels were used in typical functional areas of a university campus in Beijing, China, including offices, dormitories, leisure spaces, canteens, and laboratories. By comparing the findings with data from nearby national monitoring stations, the seasonal and spatial variations in PM2.5 and CO2 concentrations were analyzed. Findings indicate PM2.5 levels within the campus were notably lower compared to the surrounding urban environment. There was variation in PM2.5 and CO2 concentrations across different functional areas. Typically, indoor PM2.5 levels were lower than outdoor ones, while CO2 concentrations in enclosed indoor spaces with human activities progressively escalated. The main internal emission sources affecting the PM2.5 level on campus included traffic emissions, dust generated by human activities, and emissions from catering. In contrast, in areas with better green coverage or where a lake system participates in the atmospheric circulation, the PM2.5 level and CO2/PM2.5 were lower. This indicates that the cleansing impact of plants and aquatic systems is instrumental in lowering PM2.5 concentrations, offering healthier leisure spaces. Seasonal variations also impact PM2.5 levels. During the non-heating period, less pollution source emissions led to decreased outdoor PM2.5 concentrations. The campus monitoring sites experienced an approximate 5 µg/m3 and 29 µg/m3 reduction in the average PM2.5 levels as compared to the PM2.5 of the surrounding urban environment, respectively, during the non-heating and heating period. During indoor activities or sleep, CO2 levels can build up to as high as 2303 ppm due to breathing. It is advisable to stay indoors on days when pollution levels are high, whereas on days with clean air, it is healthier to be outdoors or to air out indoor areas by opening windows. Our research provides clearer scientific evidence for incorporating behavioral strategies for improving air quality into both daily work and life. Moreover, the findings are quite meaningful for the widespread adoption of low-cost sensor monitoring in various environments, with applications beyond just the campus settings. Full article
(This article belongs to the Section Air Quality)
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14 pages, 7080 KiB  
Article
The Impact of Changing Climate on an Endangered Epiphytic Orchid (Pleione formosana) in a Montane Cloud Forest and the Conservation Challenge Ahead
by Rebecca C.-C. Hsu, Yi-Chiann Chen and Chienyu Lin
Plants 2024, 13(17), 2414; https://doi.org/10.3390/plants13172414 - 29 Aug 2024
Abstract
Pleione formosana Hayata is an endemic orchid that was once widely distributed across the mid-elevations of Taiwan. However, populations of this orchid have steadily shrunk due to orchid poaching in most of its habitats. By correlating data from micrometeorological stations that we installed [...] Read more.
Pleione formosana Hayata is an endemic orchid that was once widely distributed across the mid-elevations of Taiwan. However, populations of this orchid have steadily shrunk due to orchid poaching in most of its habitats. By correlating data from micrometeorological stations that we installed in the cloud forest canopy at the study site, Yuanyang Lake (YYL) from 2017, we discovered the critical role of spring rainfall in triggering flowering and summer rainfall in promoting the growth of new bulbs. We found that rising temperatures and frequent drought events threaten orchid growth, potentially leading to pathogen infections. We climbed old-growth yellow cypresses to collect seed capsules of P. formosana for in vitro germination at YYL beginning in the autumn of 2018. Orchid plantlets were reintroduced to the study site in mid-August of 2022. However, the seedlings failed to survive the summer of 2023. This study is the first persistent monitoring of this rare orchid in the forest canopy of this old-growth cloud forest. Based on the result, we propose conservation strategies and directions for protecting this orchid on a regional scale. Our study highlights the mounting challenge to conservation efforts posed by global climate change. Full article
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20 pages, 11742 KiB  
Article
Distribution Characteristics and Hydrocarbon Significance of Deep-Water Fine-Grained Sedimentary Rocks in the Steep-Slope Zone of a Graben Lake Basin: A Case Study of Es3l sub-Member in the Jiyang Depression, Bohai Bay Basin, China
by Qi Zhong, Wangpeng Li, Hui Huang, Jianhui Jiang, Jianguo Zhang, Pinxie Li, Yali Liu, Jiabin Wu, Fenghua Wang, Bintian Tan and Ruo Jia
Minerals 2024, 14(9), 882; https://doi.org/10.3390/min14090882 - 29 Aug 2024
Abstract
The high exploration and development production capacity of the Jiyang Depression, Bohai Bay Basin, China in the early stage confirms the huge exploration and development potential of shale oil in the study area. Due to the complexity of the depositional mechanism in the [...] Read more.
The high exploration and development production capacity of the Jiyang Depression, Bohai Bay Basin, China in the early stage confirms the huge exploration and development potential of shale oil in the study area. Due to the complexity of the depositional mechanism in the study area, the distribution law of fine-grained sedimentary rocks is not well understood, which restricts further exploration breakthroughs. This paper comprehensively observes rock cores and thin sections, combines mineral components, Rock-Eval pyrolysis, rock-cutting logging and logging data to classify lithofacies, and clarifies the distribution law of various lithofacies. The research results show that, according to lithological characteristics, various lithofacies origins are classified into three categories: terrigenous, mixed, and endogenous sources, and six lithofacies types are distinguished: terrigenous low-organic-matter massive siltstone (LF1), terrigenous low-organic-matter massive mudstone (LF2), mixed-source medium-organic-matter massive mudstone (LF3), mixed-source medium-to-high-organic matter laminated-massive mudstone (LF4), mixed-source medium-to-high-organic-matter laminated mudstone (LF5), and endogenous-sourced medium-to-high-organic matter laminated limestone (LF6). The distribution of lithofacies in plane is symmetrical in the east–west direction and is characterized by a banded distribution; the distribution in profile shows a stable depositional process and a continuous depositional sequence. The various lithofacies depositional models have been summarized; the terrigenous input from the northern steep-slope zone has influenced the hydrodynamic conditions of the lake basin, significantly affecting the lithofacies depositional variations from the steep-slope zone to the deep-sag area. The geological evaluation of each lithofacies has been conducted; LF1 + LF4 + LF5 are classified as Class I—target reservoirs for shale oil development, while LF3 + LF6 are considered Class II—favorable reservoirs. The result of the study provide a reference for the classification of fine-grained sedimentary-rock facies and distribution characteristics, and the evaluation of shale-oil-reservoir sweet spots in graben lake basins. Full article
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12 pages, 4046 KiB  
Article
Harvest of Myriophyllum spicatum Facilitates the Growth of Vallisneria denseserrulata but Has No Significant Effects on Water Quality in a Mesocosm Experiment
by Zhenmei Lin, Jian Gao, Qianhong Wang, Wei Zhen, Yiming Gao, Xiaolong Huang, Hu He, Baohua Guan, Kuanyi Li, Zhengwen Liu and Jinlei Yu
Water 2024, 16(17), 2441; https://doi.org/10.3390/w16172441 (registering DOI) - 29 Aug 2024
Abstract
The recovery of submerged macrophytes is crucial for lake restoration. However, Myriophyllum spicatum usually shows an overgrowth and inhibits the growth of Vallisneria denseserrulata via light shading in many restored shallow lakes after the plant transplantation. So far, harvesting M. spicatum is the [...] Read more.
The recovery of submerged macrophytes is crucial for lake restoration. However, Myriophyllum spicatum usually shows an overgrowth and inhibits the growth of Vallisneria denseserrulata via light shading in many restored shallow lakes after the plant transplantation. So far, harvesting M. spicatum is the primary method to alleviate these shading effects in post-restoration lakes. Nevertheless, the effects of harvesting on the growth of V. denseserrulata and water quality are poorly elaborated. In this study, we conducted a mesocosm experiment, including both monoculture and polyculture groups, to investigate the response of V. denseserrulata growth, light climate, and nutrient concentrations in the water with M. spicatum harvesting. Moreover, the growth and morphology of M. spicatum were also examined. We hypothesized that M. spicatum harvesting would enhance the growth of V. denseserrulata and improve both the light climate and water quality. Our results showed that harvesting M. spicatum in the polyculture mesocosms substantially enhanced the relative growth rate (RGR) of V. denseserrulata compared to the non-harvesting controls. Moreover, harvesting M. spicatum reduced the light attenuation coefficient at 30 cm depth; however, the concentrations of chlorophyll-a, total nitrogen, and total phosphorus did not change significantly. As for M. spicatum, harvesting inhibited the growth of main stem and root but did not significantly affect the cumulative weight and RGR of M. spicatum. In contrast, the presence of V. denseserrulata decreased cumulative weight and RGR while promoting the root parameters of M. spicatum. Our findings imply that harvesting overgrowth nuisance submerged macrophyte species (e.g., M. spicatum) can improve the light climate and reduce its root growth, thereby enhancing the growth of target macrophyte species like V. denseserrulata without changes in the water quality which provides valuable insights for post-restoration lake management. Full article
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11 pages, 723 KiB  
Article
Sulfated Aeruginosins from Lake Kinneret: Microcystis Bloom, Isolation, Structure Elucidation, and Biological Activity
by Shira Weisthal Algor, Assaf Sukenik and Shmuel Carmeli
Mar. Drugs 2024, 22(9), 389; https://doi.org/10.3390/md22090389 - 28 Aug 2024
Viewed by 184
Abstract
Aeruginosins are common metabolites of cyanobacteria. In the course of re-isolation of the known aeruginosins KT608A and KT608B for bioassay studies, we isolated three new sulfated aeruginosins, named aeruginosins KT688 (1), KT718 (2), and KT575 (3), from [...] Read more.
Aeruginosins are common metabolites of cyanobacteria. In the course of re-isolation of the known aeruginosins KT608A and KT608B for bioassay studies, we isolated three new sulfated aeruginosins, named aeruginosins KT688 (1), KT718 (2), and KT575 (3), from the extract of a Microcystis cell mass collected during the 2016 spring bloom event in Lake Kinneret, Israel. The structures of the new compounds were established on the basis of analyses of the 1D and 2D NMR, as well as HRESIMS data. Marfey’s method, coupled with HR ESI LCMS and chiral HPLC, was used to establish the absolute configuration of the amino acid and hydroxyphenyl lactic acid residues, respectively. Compounds 13 were tested for inhibition of the serine protease trypsin, and compounds 1 and 2 were found to exhibit IC50 values of 2.38 and 1.43 µM, respectively. Full article
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19 pages, 6475 KiB  
Article
Diversity, Stability, and the Forecast Challenge in Forest Lepidopteran Predictive Ecology: Are Multi-Scale Plant–Insect Interactions the Key to Increased Forecast Precision?
by Barry J. Cooke
Forests 2024, 15(9), 1501; https://doi.org/10.3390/f15091501 - 28 Aug 2024
Viewed by 221
Abstract
I report on long-term patterns of outbreak cycling in four study systems across Canada and illustrate how forecasting in these systems is highly imprecise because of complexity in the cycling and a lack of spatial synchrony amongst sample locations. I describe how a [...] Read more.
I report on long-term patterns of outbreak cycling in four study systems across Canada and illustrate how forecasting in these systems is highly imprecise because of complexity in the cycling and a lack of spatial synchrony amongst sample locations. I describe how a range of bottom-up effects could be generating complexity in these otherwise periodic systems. (1) The spruce budworm in Québec exhibits aperiodic and asynchronous behavior at fast time-scales, and a slow modulation of cycle peak intensity that varies regionally. (2) The forest tent caterpillar across Canada exhibits eruptive spiking behavior that is aperiodic locally, and asynchronous amongst regions, yet aggregates to produce a pattern of periodic outbreaks. In Québec, forest tent caterpillar cycles differ in the aspen-dominated northwest versus the maple-dominated southeast, with opposing patterns of cycle intensity between the two regions. (3) In Alberta, forest tent caterpillar outbreak cycles resist synchronization across a forest landscape gradient, even at very fine spatial scales, resulting in a complex pattern of cycling that defies simple forecasting techniques. (4) In the Border Lakes region of Ontario and Minnesota, where the two insect species coexist in a mixedwood landscape of hardwood and conifers, outbreak cycle intensity in each species varies spatially and temporally in response to host forest landscape structure. Much attention has been given to the effect of top-down agents in driving synchronizable population cycles. However, foliage loss, tree death, and forest succession at stem, stand, and landscape scales affect larval and adult dispersal success, and may serve to override regulatory processes that cause otherwise top-down-driven periodic, synchronized, and predictable population oscillations to become aperiodic, asynchronous, and unpredictable. Incorporating bottom-up effects at multiple spatial and temporal scales may be the key to making significant improvements in forest insect outbreak forecasting. Full article
(This article belongs to the Special Issue Plant-Insect Interactions in Forests)
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26 pages, 6325 KiB  
Article
Acquisition of Bathymetry for Inland Shallow and Ultra-Shallow Water Bodies Using PlanetScope Satellite Imagery
by Aleksander Kulbacki, Jacek Lubczonek and Grzegorz Zaniewicz
Remote Sens. 2024, 16(17), 3165; https://doi.org/10.3390/rs16173165 - 27 Aug 2024
Viewed by 295
Abstract
This study is structured to address the problem of mapping the bottom of shallow and ultra-shallow inland water bodies using high-resolution satellite imagery. These environments, with their diverse distribution of optically relevant components, pose a challenge to traditional mapping methods. The study was [...] Read more.
This study is structured to address the problem of mapping the bottom of shallow and ultra-shallow inland water bodies using high-resolution satellite imagery. These environments, with their diverse distribution of optically relevant components, pose a challenge to traditional mapping methods. The study was conducted on several research issues, each focusing on a specific aspect of the SDB, related to the selection of spectral bands and regression models, regression models creation, evaluation of the influence of the number and spatial distribution of reference soundings, and assessment of the quality of the bathymetric surface, with a focus on microtopography. The study utilized basic empirical techniques, incorporating high-precision reference data acquired via an unmanned surface vessel (USV) integrated with a single-beam echosounder (SBES), and Global Navigation Satellite System (GNSS) receiver measurements. The performed investigation allowed the optimization of a methodology for bathymetry acquisition of such areas by identifying the impact of individual processing components. The first results indicated the usefulness of the proposed approach, which can be confirmed by the values of the obtained RMS errors of elaborated bathymetric surfaces in the range of up to several centimeters in some study cases. The work also points to the problematic nature of this type of study, which can contribute to further research into the application of remote sensing techniques for bathymetry, especially during acquisition in optically complex waters. Full article
(This article belongs to the Section Environmental Remote Sensing)
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20 pages, 2143 KiB  
Article
Maintenance of High Phytoplankton Diversity in the Danubian Floodplain Lake over the Past Half-Century
by Melita Mihaljević, Dubravka Špoljarić Maronić, Filip Stević, Tanja Žuna Pfeiffer and Vanda Zahirović
Plants 2024, 13(17), 2393; https://doi.org/10.3390/plants13172393 - 27 Aug 2024
Viewed by 213
Abstract
Riverine floodplains are recognized as centers of biodiversity, but due to intense anthropogenic pressures, many active floodplains have disappeared during the last century. This research focuses on the long-term changes in phytoplankton diversity in the floodplain lake situated in the Kopački Rit (Croatia), [...] Read more.
Riverine floodplains are recognized as centers of biodiversity, but due to intense anthropogenic pressures, many active floodplains have disappeared during the last century. This research focuses on the long-term changes in phytoplankton diversity in the floodplain lake situated in the Kopački Rit (Croatia), one of the largest conserved floodplains in the Middle Danube. The recent dataset from 2003 to 2016 and historical data from the 1970s and 1980s indicate high phytoplankton diversity, summarising 680 taxa for nearly half a century. The variability of species richness is driven by specific in-lake variables, particularly water temperature, water depth, total nitrogen, pH, and transparency, determined by a redundancy analysis of the current data. The high phytoplankton diversity levels are sustained regardless of intense pressures on the lake environment, including exposure to strong anthropogenic pollution in the past and extreme hydrological events, both droughts and floods, which have increasingly affected this part of the Danube in the last decades. The conserved hydrological connection between various biotopes along the river–floodplain gradient seems crucial in maintaining high phytoplankton diversity. Accordingly, conserving natural flooding is mandatory to maintain high biodiversity in complex and dynamic river–floodplain systems. Full article
(This article belongs to the Special Issue Phytoplankton Community Structure and Succession)
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24 pages, 13634 KiB  
Article
Exploring Factors Affecting the Performance of Neural Network Algorithm for Detecting Clouds, Snow, and Lakes in Sentinel-2 Images
by Kaihong Huang, Zhangli Sun, Yi Xiong, Lin Tu, Chenxi Yang and Hangtong Wang
Remote Sens. 2024, 16(17), 3162; https://doi.org/10.3390/rs16173162 - 27 Aug 2024
Viewed by 324
Abstract
Detecting clouds, snow, and lakes in remote sensing images is vital due to their propensity to obscure underlying surface information and hinder data extraction. In this study, we utilize Sentinel-2 images to implement a two-stage random forest (RF) algorithm for image labeling and [...] Read more.
Detecting clouds, snow, and lakes in remote sensing images is vital due to their propensity to obscure underlying surface information and hinder data extraction. In this study, we utilize Sentinel-2 images to implement a two-stage random forest (RF) algorithm for image labeling and delve into the factors influencing neural network performance across six aspects: model architecture, encoder, learning rate adjustment strategy, loss function, input image size, and different band combinations. Our findings indicate the Feature Pyramid Network (FPN) achieved the highest MIoU of 87.14%. The multi-head self-attention mechanism was less effective compared to convolutional methods for feature extraction with small datasets. Incorporating residual connections into convolutional blocks notably enhanced performance. Additionally, employing false-color images (bands 12-3-2) yielded a 4.86% improvement in MIoU compared to true-color images (bands 4-3-2). Notably, variations in model architecture, encoder structure, and input band combination had a substantial impact on performance, with parameter variations resulting in MIoU differences exceeding 5%. These results provide a reference for high-precision segmentation of clouds, snow, and lakes and offer valuable insights for applying deep learning techniques to the high-precision extraction of information from remote sensing images, thereby advancing research in deep neural networks for semantic segmentation. Full article
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11 pages, 2538 KiB  
Article
Myxobolus dabryi n. sp. (Myxozoa: Myxobolidae) Infecting the Gills of Chanodichthys dabryi, Bleeker, 1871 (Cypriniformes: Cyprinidae) in Hunan Province, China
by Xiaojing Zhao, Qi Yin, Jia Cai, Qiang Wei, Deliang Li, Jianbo Yu, Jianguo Xiang, Jinyong Zhang and Xinhua Liu
Animals 2024, 14(17), 2487; https://doi.org/10.3390/ani14172487 - 27 Aug 2024
Viewed by 205
Abstract
Culters are a popular and economically important carnivorous freshwater fish, widely distributed in rivers, lakes, and reservoirs in China. An investigation of Myxozoa was conducted to enhance the understanding of Myxozoan diversity in Culters in China, as only 15 Myxosporean species have been [...] Read more.
Culters are a popular and economically important carnivorous freshwater fish, widely distributed in rivers, lakes, and reservoirs in China. An investigation of Myxozoa was conducted to enhance the understanding of Myxozoan diversity in Culters in China, as only 15 Myxosporean species have been previously reported in 6 Culters species. A new species with typical Myxobolus characteristics was discovered exclusively in the gills of Chanodichthys dabryi, Bleeker, 1871, and no other species were found in other Culters fish or organs. The new species elicited whitish plasmodia in the serosa layer of the gill arch, with no distinct inflammatory reaction observed. This species is morphologically different from all reported Myxobolus spp. from Culters, differing in plasmodium and spore size, as well as the coils of polar filaments. Molecular analysis further supports that it does not match any sequences available in GenBank. Therefore, we identified it as a new species and named it Myxobolus dabryi n. sp. Full article
(This article belongs to the Section Aquatic Animals)
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18 pages, 759 KiB  
Article
Adaptive Channel Estimation Based on Multidirectional Structure in Delay-Doppler Domain for Underwater Acoustic OTFS System
by Wentao Shi, Mingqi Jin, Lianyou Jing, Nan Tu and Chengbing He
Remote Sens. 2024, 16(17), 3157; https://doi.org/10.3390/rs16173157 - 27 Aug 2024
Viewed by 194
Abstract
Time-varying underwater acoustic (UWA) channels are the key challenge of underwater acoustic communication (UAC). Although UAC exhibits time-variance characteristics significantly in time domains, its delay-Doppler (DD) domain representation tends to be time-invariant. Orthogonal time–frequency space (OTFS) modulation has recently been proposed and has [...] Read more.
Time-varying underwater acoustic (UWA) channels are the key challenge of underwater acoustic communication (UAC). Although UAC exhibits time-variance characteristics significantly in time domains, its delay-Doppler (DD) domain representation tends to be time-invariant. Orthogonal time–frequency space (OTFS) modulation has recently been proposed and has acquired widespread interest due to its excellent performance over time-varying channels. In the UWA OTFS system, the novel DD domain channel estimation algorithm that employs a multidirectional adaptive moving average scheme is proposed. Specifically, the proposed scheme is cascaded by a channel estimator and moving average filter. The channel estimator can be employed to estimate the time-invariant channel of the DD domain multidirectionally, improving proportionate normalized least mean squares (IPNLMS). Meanwhile, the moving average filter is used to reduce the output noise of the IPNLMS. The performance of the proposed method is verified by simulation experiments and real-world lake experiments. The results demonstrate that the proposed channel estimation method can outperform those of benchmark algorithms. Full article
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13 pages, 14098 KiB  
Article
Pit Lakes in Abandoned Slate Quarries in Northwestern Spain: Characteristics and Potential Uses
by José María Redondo-Vega, Javier Santos-González, Adrián Melón-Nava, Amelia Gómez-Villar, Sergio A. Peña-Pérez and Rosa Blanca González-Gutiérrez
Water 2024, 16(17), 2403; https://doi.org/10.3390/w16172403 - 27 Aug 2024
Viewed by 314
Abstract
Human activities play a key role in landscape evolution. Mining is one of the most important of these since it changes large parts of the Earth’s surface. In abandoned mines and quarries, pit lakes are quite common due to water table blocking. These [...] Read more.
Human activities play a key role in landscape evolution. Mining is one of the most important of these since it changes large parts of the Earth’s surface. In abandoned mines and quarries, pit lakes are quite common due to water table blocking. These lakes have remarkable ecological characteristics, but they are still poorly understood. Northwestern Spain has a long tradition in the use of slate for roofs. This exploitation, mostly open-pit, has caused a great transformation in the landscape including the generation of pit lakes. In this work, 27 pit lakes in abandoned slate quarries areas have been analysed using orthophotos of different years, UAV (unmanned aerial vehicle) images and field work. In each lake, the topographic context, geomorphological dynamics, morphometric parameters (including depth), pH, and ecological variables were measured. Other uses for these quarry pit lakes and their significance as a new geo-ecological environment are also discussed. The results show different types of pit lakes and, consequently, different potential uses in each case. Only six offer good conditions for recreational uses, whereas the rest (21) show slope instability or poor water quality. Full article
(This article belongs to the Section Water Resources Management, Policy and Governance)
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25 pages, 3325 KiB  
Article
Effects of Paddy Rain-Flood Storage on Rice Growth Physiological Indices and Nitrogen Leaching under Organic Planting in Erhai Lake Basin
by Qingsheng Liu, Qiling Lu, Liudong Zhang, Shufang Wang, Aiqing Zou, Yong Su, Jun Sha, Ying Wang and Lihong Chen
Plants 2024, 13(17), 2381; https://doi.org/10.3390/plants13172381 - 26 Aug 2024
Viewed by 333
Abstract
In order to address the increasingly prominent issues of water resource protection and agricultural non-point source pollution in the Erhai Lake Basin, this study conducted a two-year field experiment in Gusheng Village, located in the Erhai Lake Basin. In 2022, two irrigation treatments [...] Read more.
In order to address the increasingly prominent issues of water resource protection and agricultural non-point source pollution in the Erhai Lake Basin, this study conducted a two-year field experiment in Gusheng Village, located in the Erhai Lake Basin. In 2022, two irrigation treatments were set up: conventional flooding irrigation (CK) and controlled irrigation (C), with three replicates for each treatment. In 2023, aiming to enhance the utilization rate of rainwater resources and reduce the direct discharge of dry-farming tailwater from upstream into Erhai Lake. The paddy field was used as an ecological storage basin, and the water storage depth of the paddy field was increased compared to the depth of 2022. Combined with the deep storage of rainwater, the dry-farming tailwater was recharged into the paddy field to reduce the drainage. In 2023, two water treatments, flooding irrigation with deep storage and controlled drainage (CKCD) and water-saving irrigation with deep storage and controlled drainage (CCD) were set up, and each treatment was set up with three replicates. The growth and physiological index of rice at various stages were observed. Nitrogen leaching of paddy field in surface water, soil water, and groundwater under different water treatments after tillering fertilizer were observed. The research results show that the combined application of organic and inorganic fertilizers under organic planting can provide more reasonable nutrient supply for rice, promote dry matter accumulation and other indices, and also reduce the concentration of NH4+-N in surface water. Compared with CK, the yield, 1000-grain weight, root-to-shoot ratio, and leaf area index of C are increased by 4.8%, 4.1%, 20.9%, and 9.7%, respectively. Compared with CKCD, the yield, 1000-grain weight, root-to-shoot ratio, and leaf area index of CCD are increased by 6.5%, 3.8%, 19.6%, and 21.9%, respectively. The yield in 2023 is 19% higher than that in 2022. Treatment C can increase the growth indicators and reduce the net photosynthetic rate to a certain extent, while CCD rain-flood storage can alleviate the inhibition of low irrigation lower limit on the net photosynthetic rate of rice. Both C and CCD can reduce nitrogen loss and irrigation amount in paddy fields. CCD can reduce the tailwater in the Gusheng area of the Erhai Lake Basin to Erhai Lake, and also can make full use of N, P, and other nutrients in the tailwater to promote the formation and development of rice. In conclusion, the paddy field rain-flood storage methodology in the Erhai Lake Basin can promote various growth and physiological indicators of rice, improve water resource utilization efficiency, reduce direct discharge of tailwater into Erhai Lake, and decrease the risk of agricultural non-point source pollution. Full article
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17 pages, 5605 KiB  
Article
Green and Low Carbon Development Performance in Farmland Use Regulation: A Case Study of Liyang City, China
by Yaoben Lin, Xuewen Wang, Guangyu Li and Wei Shen
Land 2024, 13(9), 1365; https://doi.org/10.3390/land13091365 - 26 Aug 2024
Viewed by 241
Abstract
Farmland use regulation strictly regulates the conversion of agricultural land for other agricultural purposes and the construction of agricultural facilities, thereby optimizing the land use pattern in rural areas. However, different measures and intensities of farmland use regulation can affect the overall performance [...] Read more.
Farmland use regulation strictly regulates the conversion of agricultural land for other agricultural purposes and the construction of agricultural facilities, thereby optimizing the land use pattern in rural areas. However, different measures and intensities of farmland use regulation can affect the overall performance of green and low-carbon development in rural areas. This study utilizes system dynamics modeling and simulation to conduct a case study based on current land use data from 10 towns in Liyang City, China. The empirical results indicate the following: (1) Based on comprehensive measurements of green and low carbon development performance, Liyang City exhibits a pattern of higher indices in the south and lower indices in the north. Towns such as Tianmu Lake, Daibu, and Shezhu show relatively high average comprehensive indices of 0.31, 0.30, and 0.28, significantly higher than other towns. (2) Simulation of farmland use regulation’s impact on green and low carbon development performance reveals that Scenario One, involving additional construction land occupying farmland, achieves a comprehensive index of only 0.23, significantly lower than the other scenarios. (3) Based on calculations and field surveys, Liyang City’s villages are categorized into four types, with the largest number being industry-integrated villages (94 villages). Accordingly, policies for farmland use regulation are designed for different village types. Therefore, future farmland use regulation should be tailored with differentiated institutional designs according to the development needs of different villages. This study’s findings provide insights into green and low-carbon development in rural areas. Full article
(This article belongs to the Special Issue Advances in Land Consolidation and Land Ecology)
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20 pages, 4621 KiB  
Article
Global Distribution and Diversity of Haloarchaeal pL6-Family Plasmids
by Mike Dyall-Smith and Friedhelm Pfeiffer
Genes 2024, 15(9), 1123; https://doi.org/10.3390/genes15091123 - 26 Aug 2024
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Abstract
Australian isolates of Haloquadratum walsbyi, a square-shaped haloarchaeon, often harbor small cryptic plasmids of the pL6-family, approximately 6 kb in size, and five examples have been previously described. These plasmids exhibit a highly conserved gene arrangement and encode replicases similar to those [...] Read more.
Australian isolates of Haloquadratum walsbyi, a square-shaped haloarchaeon, often harbor small cryptic plasmids of the pL6-family, approximately 6 kb in size, and five examples have been previously described. These plasmids exhibit a highly conserved gene arrangement and encode replicases similar to those of betapleolipoviruses. To assess their global distribution and recover more examples for analysis, fifteen additional plasmids were reconstructed from the metagenomes of seven hypersaline sites across four countries: Argentina, Australia, Puerto Rico, and Spain. Including the five previously described plasmids, the average plasmid size is 6002 bp, with an average G+C content of 52.5%. The tetramers GGCC and CTAG are either absent or significantly under-represented, except in the two plasmids with the highest %G+C. All plasmids share a similar arrangement of genes organized as outwardly facing replication and ATPase modules, but variations were observed in some core genes, such as F2, and some plasmids had acquired accessory genes. Two plasmids, pCOLO-c1 and pISLA-c6, shared 92.7% nt identity despite originating from Argentina and Spain, respectively. Numerous metagenomic CRISPR spacers matched sequences in the fifteen reconstructed plasmids, indicating frequent invasion of haloarchaea. Spacers could be assigned to haloarchaeal genera by mapping their associated direct repeats (DR), with half of these matching Haloquadratum. Finally, strand-specific metatranscriptome (RNA-seq) data could be used to demonstrate the active transcription of two pL6-family plasmids, including antisense transcripts. Full article
(This article belongs to the Section Microbial Genetics and Genomics)
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