Search Results (62)

We aimed to (1) compare external training loads obtained in futsal players during pre-season and in-season training weeks to explore how these loads are periodized by the coaching staff; and (2) compare the external training loads using the match day minus approach during the pre-season and in-season periods to determine how the within-microcycle training load varies between these periods. A total of 432 recordings were obtained of twelve national-level male amateur players using IPS devices. These data were collected over four consecutive microcycles from the beginning to the end of the pre-season, as well as during four additional consecutive microcycles at the start of the in-season. The external load variables evaluated were: total distance covered (TD; m), relative distance covered (Drel; m/min), high-speed running (HSR; m > 18.8 km/h), sprint distance (SPR; m > 25.2 km/h), number of intense accelerations (ACC; >2.5 m/s²) and decelerations (DEC; >−2.5 m/s²). Results indicated significant week-by-week variation (p < 0.05) in all external load variables during the pre-season. During the in-season, significant variations (p < 0.05) were observed only in Drel, HSR, and ACC. In both phases, all variables were higher (p < 0.05) two days before the match. In conclusion, it is important to consistently quantify the external loads of futsal players to ensure appropriate variation in loading and tapering within the microcycle, as well as during the pre-season and in-season periods. Full article

Rugby players must develop excellent levels of conditioning during adolescence. However, this pivotal period of life is also characterized by a surge in biological growth, which further increases the energy and nutritional requirements of this population. This study examined within-individual differences in energy intake (EI) and energy balance (EB) of 46 young rugby players during a pre-season micro-cycle. Two clusters were identified with significantly different characteristics and EB states, suggesting that young rugby players adjust their EI to match their body composition goals. The first cluster is characterized by players with a low body fat% (12.87 ± 2.53). They had a positive EB (330 ± 517 kcal), suggesting a goal of increasing muscle mass. Conversely, the second cluster is characterized by a higher body fat% (23.1 ± 1.6, p < 0.005) and reported a negative, lower EB (−683 ± 425 kcal, p < 0.005), suggesting a goal focused on reducing fat mass. Although our study provides more optimistic results than previous ones regarding the high risk of inadequate EI in young rugby players, we emphasize the importance of rigorous nutritional support, especially for players aiming to lose weight, to avoid severe caloric restriction, as well as the downstream effects of such practices on their nutritional status, given the higher risk of macro- (e.g., CHO < 6 g/kg/d) and micronutrient (e.g., iron < 11 mg/d, calcium < 1300 mg/d, vitamin D < 5 mg/d) deficiencies. Full article

The purpose of this study was to assess the external load demands in futsal, considering both home and away matches and their outcomes, in order to plan microcycles throughout the season based on the external load of each match. The external load of 10 players from a First Division team in the Spanish Futsal League was recorded throughout 15 official matches in the first half of the league championship. The players’ external load was monitored using OLIVER devices. To analyse the influence of the match outcome and location on the external load, a univariate general linear model (GLM) analysis was conducted with Bonferroni post hoc. There are no differences between the variables neither comparing results nor location factors, except for accelerations of 2 to 3 m/s² (m) per minute and the number of accelerations of 2 to 3 m/s² per minute, reporting higher value winnings at home than away (p < 0.05). The location and results are not factors that influence on external load in futsal matches, except the number and distance performed in accelerations and distance covered at a low to medium speed. These findings are important for planning microcycles and providing the appropriate dosage to each player to achieve optimal performance in matches. Full article

Insect pathogenic fungi have shown great potential in agricultural pest control. Conidiation is crucial for the survival of filamentous fungi, and dispersal occurs through two methods: normal conidiation, where conidia differentiate from mycelium, and microcycle conidiation, which involves conidial budding. The conidiation process is related to cell separation. The forkhead box gene Sep1 in Schizosaccharomyces pombe plays a crucial role in cell separation. Nevertheless, the function of Sep1 has not been clarified in filamentous fungi. Here, MaSep1, the homolog of Sep1 in Metarhizium acridum, was identified and subjected to functional analysis. The findings revealed that conidial germination of the MaSep1-deletion strain (ΔMaSep1) was accelerated and the time for 50% germination rate of conidial was shortened by 1 h, while the conidial production of ΔMaSep1 was considerably reduced. The resistances to heat shock and UV-B irradiation of ΔMaSep1 were enhanced, and the expression of some genes involved in DNA damage repair and heat shock response was significantly increased in ΔMaSep1. The disruption of MaSep1 had no effect on the virulence of M. acridum. Interestingly, ΔMaSep1 conducted the normal conidiation on the microcycle conidiation medium, SYA. Furthermore, 127 DEGs were identified by RNA-Seq between the wild-type and ΔMaSep1 strains during microcycle conidiation, proving that MaSep1 mediated the conidiation pattern shift by governing some genes associated with conidiation, cell division, and cell wall formation. Full article

Perceived exertion (RPE, RPE_dur) and wellness scores (Hooper) are common methods to assess the training load and readiness in football. However, in professional football, there is a lack of data concerning the application of these tools in microcycles of different durations. Therefore, the purpose of this study was to explore the correlations between (a) RPE, RPE_dur, and weekly external load (EXLD) and (b) Hooper and EXLD within microcycles of different durations. Independent of the microcycle’s length, RPE and RPE_dur correlated with the EXLD. In MIC5, RPE and RPE_dur correlated with the EXLD, except for accelerations > 3 m/s² (ACh) and distance > 25.5 km/h (SPvh). In MIC6, RPE and RPE_dur correlated with the EXLD, except Ach (with RPE). In MIC7 and MIC9, RPE and RPE_dur correlated with the EXLD, except RPE with ACh. For Hooper variables, independent of the microcycle duration, SRN and FTG had more correlations in comparison to SLP; the same finding was observed in MIC7 and MIC9, but SRN and SLP in MIC5 and MIC6, respectively, had no correlations. Multiple regression analysis showed that for RPE, the main predictors are total distance (TD), metabolic power distance > 50 w/kg (PWvh), and ACh, which can explain 31% of the variance, but for RPE_dur, 58% can be explained by DUR, TD, and PWvh. For SLP, the predictors FTG and SRN can explain a very small percentile of the variance (1%, 4%, and 6%, respectively). The main findings were the multiple correlations between RPE, RPE_dur, Hooper, and microcycles of different durations; most of them were found for long durations. This tendency is similar to that described in the literature for microcycles of the same duration. (a) EXLD has a stronger correlation with RPE_dur than with RPE, and (b) FTG and SRN are the best predictors of the EXLD during the upcoming training session. It is recommended that fitness coaches use these tools to assess and forecast the training load; thus, they will be able to prepare more effective sessions during microcycles of different durations. Full article

Utilizing techniques for reducing multivariate data is essential for comprehensively understanding the variations and relationships within both biomechanical and physiological datasets in the context of youth football training. Therefore, the objective of this study was to identify the primary factors influencing training sessions within a standard microcycle among young sub-elite football players. A total of 60 male Portuguese youth sub-elite footballers (15.19 ± 1.75 years) were continuous monitored across six weeks during the 2019–2020 in-season, comprising the training days from match day minus (MD-) 3, MD-2, and MD-1. The weekly training load was collected by an 18 Hz global positioning system (GPS), 1 Hz heart rate (HR) monitors, the perceived exertion (RPE) and the total quality recovery (TQR). A principal component approach (PCA) coupled with a Monte Carlo parallel analysis was applied to the training datasets. The training datasets were condensed into three to five principal components, explaining between 37.0% and 83.5% of the explained variance (proportion and cumulative) according to the training day (p < 0.001). Notably, the eigenvalue for this study ranged from 1.20% to 5.21% within the overall training data. The PCA analysis of the standard microcycle in youth sub-elite football identified that, across MD-3, MD-2, and MD-1, the first was dominated by the covered distances and sprinting variables, while the second component focused on HR measures and training impulse (TRIMP). For the weekly microcycle, the first component continued to emphasize distance and intensity variables, with the ACC and DEC being particularly influential, whereas the second and subsequent components included HR measures and perceived exertion. On the three training days analyzed, the first component primarily consisted of variables related to the distance covered, running speed, high metabolic load, sprinting, dynamic stress load, accelerations, and decelerations. The high intensity demands have a high relative weight throughout the standard microcycle, which means that the training load needs to be carefully monitored and managed. Full article

The purpose of this study was to (a) correlate the weekly external training load with the game running performance in season microcycles and (b) specify the optimal training/game ratio of the weekly external load in elite youth soccer players. The total distance (TD), the high-speed running distance (HSRD) (19.8–25.2 km/h), the ZONE6 distance (>25.2 km/h), the acceleration (ACC) (≥+2 m/s²), and the deceleration (DEC) (≥−2 m/s²) were monitored with global positioning system (GPS) technology throughout 18 microcycles and official games. TD had a very high positive correlation average (r = 0.820, p = 0.001), the HSRD had a high positive correlation average (r = 0.658, p = 0.001), the ZONE6 distance and DEC had a moderate positive correlation average ((r = 0.473, p = 0.001) and (r = 0.478, p = 0.001), respectively), and the ACC had a low positive correlation average (r = 0.364, p = 0.001) between microcycles and games. Regarding the training/game ratio, the HSRD showed statistically significant differences between ratios 1.43 and 2.60 (p = 0.012, p ≤ 0.05), the ACC between ratios 2.42 and 4.45 (p = 0.050, p ≤ 0.05) and ratios 3.29 and 4.45 (p = 0.046, p ≤ 0.05), and the DEC between ratios 2.28 and 3.94 (p = 0.034, p ≤ 0.05). Considering the correlation between weekly training and game external load, high weekly training TD values correspond to higher game values, whereas HSRD, ZONE6 distance, ACC, and DEC, which determine training intensity, should be trained in a specific volume. Training/game ratios of 1.43, 2.42 to 3.29, and 2.28 to 3.11 seem to be optimal for HSRD, ACC, and DEC weekly training, respectively. Full article

Entomopathogenic fungi are valuable sources of biological pesticides, with conidial yield and quality being pivotal factors determining their broad applications. AzaR, a fungus-specific zinc-cluster transcription factor, is known to regulate the biosynthesis of polyketone secondary metabolites in Aspergillus niger; however, its role in pathogenic fungi remains unclear. This study investigated the role of MaAzaR in the growth, development, and environmental tolerance of Metarhizium acridum. MaAzaR deletion slowed down conidial germination rate, caused reduction in conidial yield, lowered fungal tolerance to UV radiation, did not affect fungal heat-shock tolerance, and increased fungal sensitivity to the cell-wall-destructive agent calcofluor white. Furthermore, MaAzaR deletion transformed microcycle conidiation to normal conidiation on the microcycle conidiation medium. Transcription profile analysis demonstrated that MaAzaR could regulate transformation of the conidiation pattern by controlling the expression of genes related to cell division, mycelium growth and development, and cell wall integrity. Thus, this study identified a new gene related to fungal conidiation and environmental tolerance, enriching our understanding of the molecular mechanism of microcycle conidiation and providing theoretical support and genetic resources for the development of high-yielding strains. Full article

The study presents the results of the research into the effect of the dynamic properties of inverter and diode power sources of welding arc power supply on the stability of melting and transfer of electrode metal into the weld pool. The principal energy parameters of the power source include the rates of rise and fall of short-circuit current, the ratio of arc burning current to short-circuit current, and other related factors. It has been demonstrated that an increase in the rate of change of these parameters within one welding mode microcycle alters the properties of heat and mass transfer, increases the frequency of electrode metal droplet transfer, reduces the size of transferred droplets in the weld pool and the duration of their stay on the electrode end under the influence of the high temperature of the welding arc, and the duration of short circuits. The increase in the mass fraction of alloying elements at their transition from the coated electrode to the weld metal is demonstrated to depend on the rate of change of the main energy parameters of one welding mode microcycle of the inverter power source in comparison with the diode rectifier. An enhancement in the structural integrity and properties of permanent joints during welding has been observed when using an inverter power source for the welding arc with high dynamic properties. Full article

This study analyzed workload variations (internal, external, and mental) in training sessions based on soccer players’ match participation. Thirty-six professional Brazilian players from a single team were categorized into three groups: starter (G1), non-starter (G2) and non-participant (G3) players. Monitoring during sessions involved assessing internal load via perceived exertion, mental load through affective, emotional, and motivational factors, and external load using Global Positioning System devices. On MD+2, starter players exhibited significantly lower values in all categories compared to non-starters (p < 0.001) and non-participants (p < 0.001), while on MD, they displayed the highest values. MD-4 and MD-3 showed no cognitive or emotional load differences; however, variations were observed in RPE, motivation, mental fatigue, and physical metrics. Notably, starters’ lowest values occurred on MD-2 and MD-1. Findings emphasize the need to manage starter players’ load, implementing recovery strategies for optimal performance. Additionally, tailored tasks should be provided for non-starter and non-participant players to compensate for non-performed efforts during match downtime. This study underscores the significance of an individualized training approach based on players’ match participation, contributing valuable insights for optimizing performance and recovery strategies. Full article

The aim of this study was twofold: (a) to examine the differences on physical indexes between players of different playing positions and (b) to reveal the correlations between their physical parameters and the running profile within the weekly microcycles. The subjects (n = 44) were separated into five positions: central defenders (n = 7), wide defenders (n = 9), midfielders (n = 13), wingers (n = 8) and strikers (n = 7), and all of them belonged to a professional 1st division European team during the 2020–21 and 2021–22 seasons. An incremental treadmill protocol was used to assess the VO₂max, vVO₂max and the vRER in the last week before the regular season. Then, during the first semesters, external load data were collected for two consecutive seasons to analyze the running profiles of the different positions. There were no differences on physical parameters between the players of various positions, but interestingly there were different correlations with the external load during the microcycles. Central defenders did not show any correlation. For the other positions, correlations were found between respiratory exchange ratio (vRER) and metabolic power efforts (MPWEF) (p = 0.028, r = 0.721) for wide defenders, between vRER and medium intensity accelerations (ACC2) (p = 0.004, r = 0.741) for midfielders, medium intensity decelerations (DEC2) (p = 0.01, r = 0.685), metabolic power zone 5 (MPW5) (p = 0.016, r = 0.652), MPWEF (p = 0.005, r = 0.725), and also between maximal rate of oxygen consumption (VO₂max) and high intensity decelerations (DEC3) (p = 0.045, r = 0.564), metabolic power zone 4 (MPW4) (p = 0.026, r = 0.614), MPW5 (p = 0.017, r = 0.646), MPWEF (p = 0.01, r = 0.685). A correlation between velocity on VO₂max (vVO₂max) and ACC2 (p = 0.038, r = 0.733), MPW5 (p = 0.005, r = 0.873), and MPWEF (p = 0.009, r = 0.84) was found for the wingers. Finally, there was a correlation between vRER and MPWEF (p = 0.026, r = 0.866), and between vVO₂max and high intensity accelerations (ACC3) (p = 0.03, r = 0.801) for the strikers. Our study supports that the external load of players in different positions during the training sessions is correlated with their physical indexes. These correlations are determined mostly by their tactical role, but not from their physical level, as there were no differences. Full article

Hamstring strain injuries (HSIs) are one of the most common injuries in sprint-based sports. In soccer, the ability to sprint is key, not only because of its relation to performance but also due to its possible protective effect against HSIs. Although many authors have focused on the “how”, “when”, and “what” training load should be implemented, there is a lack of practical proposals for sprint training in a high-level professional environment. The objective of this narrative review is, after a deep review of the scientific literature, to present a practical approach for sprint training, trying to answer some of the questions that most strength and conditioning coaches ask themselves when including it in soccer. Once the literature published on this topic was reviewed and combined with the practical experience of the authors, it was concluded that sprint training in soccer, although it presents an obvious need, is not something about which there is methodological unanimity. However, following the practical recommendations from this narrative review, strength and conditioning coaches can have a reference model that serves as a starting point for optimal management of the internal and external training load when they wish to introduce sprint training in the competitive microcycle in professional soccer players, with the aim of reducing HSIs. Full article

The aim of the study was to determine the effect of step load in hypoxia on the effectiveness of preoperative rehabilitation (PR) and hormone levels based on a case study. Introduction: We assessed the impact of variables such as rate of movement and time under tension (TUT) in normobaric hypoxia on the levels of growth hormone (GH), insulin-like growth factor 1 (IGF-1), and erythropoietin (EPO). Additionally, the impact of step load on the hypertrophy and strength of knee extensors and flexors was assessed. Methods: The work uses a case study, the research subject of which was a 23-year-old female professional handball player. The tests included an isokinetic assessment of the peak torque of knee extensors and flexors as well as body composition analysis. Results: The results showed a more than (10.81-fold) increase in GH after the microcycle with time under tension (TUT). The deficit between the lower limbs was also reduced. Conclusions: Using a hypoxic environment based on an appropriate altitude, combined with changes such as a short rest break between sets and a controlled tempo of movement with an eccentric phase, TUT may offer an alternative to the PR process, especially among athletes who care about fast RTS. Full article

The pre-season plays a crucial role in the preparation of professional football players, as it allows for an extensive focus on training sessions compared to the more congested schedules during the in-season period, especially in professional football leagues. This study aimed to describe the workload during a 6-week pre-season in Belgian professional football players and to analyse and compare the workloads for players in each microcycle according to several variables of external workload (e.g., distance covered at some velocities). Seventeen male Belgian professional football players competing in the second division of the Belgian league system participated in the study. Throughout the 6 weeks, the players were closely monitored during both training sessions and friendly matches using Global Positioning System (GPS) devices. Several parameters, including total distance covered and distance at different velocities, were recorded. Accelerating and decelerating distances, as well as the number of sprints, were also captured. Statistical analysis was based on a repeated measures ANOVA, percentage dynamics, and effect size calculations. The results obtained showed a progressive increase in the distance travelled at different intensities from week 1 (i.e., lower values) to week 3 (i.e., higher values), with reductions in these values in week 6, prior to the start of the official competition. Similarly, the peak of accelerations and decelerations were observed in week 2 and week 3, with decrements at the end of the pre-season period. This comprehensive investigation attempts to shed light on the effects and dynamic changes in external workload during the crucial pre-season, contributing valuable insights for coaches and practitioners in football conditioning and training programs, especially concerning optimal preparation for the beginning of the league’s season. Full article

Hamstring injuries in soccer continue to be a challenge for professionals who work with soccer players daily. Although its origin is multifactorial, the proper management of neuromuscular fatigue during the training microcycle is a very important factor to consider. There are no clear guidelines regarding the weekly distribution of certain exercises that demand the hamstrings. The main objective of this study was to describe the usual training practices of professional European soccer teams. An international observational survey design was applied to some of the strength and conditioning coaches of professional soccer teams. The survey included different neuromuscular demanding exercises for the hamstrings. For each exercise, the strength and conditioning coaches had to respond in relation to their frequency of use and timepoint depending on the day of the weekly microcycle. Although there is no strong consensus in this regard, there does seem to be a trend when applying certain exercises, especially on the days matchday-4 and matchday-3. Full article