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13 pages, 4094 KiB  
Article
Analysis of the Spatial Distribution and Common Mode Error Correlation in a Small-Scale GNSS Network
by Aiguo Li, Yifan Wang and Min Guo
Sensors 2024, 24(17), 5731; https://doi.org/10.3390/s24175731 - 3 Sep 2024
Viewed by 247
Abstract
When analyzing GPS time series, common mode errors (CME) often obscure the actual crustal movement signals, leading to deviations in the velocity estimates of station coordinates. Therefore, mitigating the impact of CME on station positioning accuracy is crucial to ensuring the precision and [...] Read more.
When analyzing GPS time series, common mode errors (CME) often obscure the actual crustal movement signals, leading to deviations in the velocity estimates of station coordinates. Therefore, mitigating the impact of CME on station positioning accuracy is crucial to ensuring the precision and reliability of GNSS time series. The current approach to separating CME mainly uses signal filtering methods to decompose the residuals of the observation network into multiple signals, from which the signals corresponding to CME are identified and separated. However, this method overlooks the spatial correlation of the stations. In this paper, we improved the Independent Component Analysis (ICA) method by introducing correlation coefficients as weighting factors, allowing for more accurate emphasis or attenuation of the contributions of the GNSS network’s spatial distribution during the ICA process. The results show that the improved Weighted Independent Component Analysis (WICA) method can reduce the root mean square (RMS) of the coordinate time series by an average of 27.96%, 15.23%, and 28.33% in the E, N, and U components, respectively. Compared to the ICA method, considering the spatial distribution correlation of stations, the improved WICA method shows enhancements of 12.53%, 3.70%, and 8.97% in the E, N, and U directions, respectively. This demonstrates the effectiveness of the WICA method in separating CMEs and provides a new algorithmic approach for CME separation methods. Full article
(This article belongs to the Section Navigation and Positioning)
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24 pages, 2745 KiB  
Article
Optimizing Glaucoma Diagnosis with Deep Learning-Based Segmentation and Classification of Retinal Images
by Nora A. Alkhaldi and Ruqayyah E. Alabdulathim
Appl. Sci. 2024, 14(17), 7795; https://doi.org/10.3390/app14177795 - 3 Sep 2024
Viewed by 228
Abstract
Glaucoma, a leading cause of permanent blindness worldwide, necessitates early detection to prevent vision loss, a task that is challenging and time-consuming when performed manually. This study proposes an automatic glaucoma detection method on enhanced retinal images using deep learning. The system analyzes [...] Read more.
Glaucoma, a leading cause of permanent blindness worldwide, necessitates early detection to prevent vision loss, a task that is challenging and time-consuming when performed manually. This study proposes an automatic glaucoma detection method on enhanced retinal images using deep learning. The system analyzes retinal images, generating masks for the optic disc and optic cup, and providing a classification for glaucoma diagnosis. We employ a U-Net architecture with a pretrained residual neural network (ResNet34) for segmentation and an EfficientNetB0 for classification. The proposed framework is tested on publicly available datasets, including ORIGA, REFUGE, RIM-ONE DL, and HRF. Our work evaluated the U-Net model with five pretrained backbones (ResNet34, ResNet50, VGG19, DenseNet121, and EfficientNetB0) and examined preprocessing effects. We optimized model training with limited data using transfer learning and data augmentation techniques. The segmentation model achieves a mean intersection over union (mIoU) value of 0.98. The classification model shows remarkable performance with 99.9% training and 100% testing accuracy on ORIGA, 99.9% training and 99% testing accuracy on RIM-ONE DL, and 98% training and 100% testing accuracy on HRF. The proposed model outperforms related works and demonstrates potential for accurate glaucoma classification and detection tasks. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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20 pages, 4340 KiB  
Article
Residual Dense Optimization-Based Multi-Attention Transformer to Detect Network Intrusion against Cyber Attacks
by Majid H. Alsulami
Appl. Sci. 2024, 14(17), 7763; https://doi.org/10.3390/app14177763 - 3 Sep 2024
Viewed by 293
Abstract
Achieving cyber-security has grown increasingly tricky because of the rising concern for internet connectivity and the significant growth in software-related applications. It also needs a robust defense system to defend itself from multiple cyberattacks. Therefore, there is a need to generate a method [...] Read more.
Achieving cyber-security has grown increasingly tricky because of the rising concern for internet connectivity and the significant growth in software-related applications. It also needs a robust defense system to defend itself from multiple cyberattacks. Therefore, there is a need to generate a method for detecting and classifying cyber-attacks. The developed model can be integrated into three phases: pre-processing, feature selection, and classification. Initially, the min-max normalization of original data was performed to eliminate the impact of maximum or minimum values on the overall characteristics. After that, synthetic minority oversampling techniques (SMOTEs) were developed to reduce the number of minority attacks. The significant features were selected using a Hybrid Genetic Fire Hawk Optimizer (HGFHO). An optimized residual dense-assisted multi-attention transformer (Op-ReDMAT) model was introduced to classify selected features accurately. The proposed model’s performance was evaluated using the UNSW-NB15 and CICIDS2017 datasets. A performance analysis was carried out to demonstrate the effectiveness of the proposed model. The experimental results showed that the UNSW-NB15 dataset attained a higher precision, accuracy, F1-score, error rate, and recall of 97.2%, 98.82%, 97.8%, 2.58, and 98.5%, respectively. On the other hand, the CICIDS 2017 achieved a higher precision, accuracy, F1-score, and recall of 98.6%, 99.12%, 98.8%, and 98.2%, respectively. Full article
(This article belongs to the Special Issue New Technology Trends in Smart Sensing)
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25 pages, 6948 KiB  
Article
Short-Term Load Forecasting for Regional Smart Energy Systems Based on Two-Stage Feature Extraction and Hybrid Inverted Transformer
by Zhewei Huang and Yawen Yi
Sustainability 2024, 16(17), 7613; https://doi.org/10.3390/su16177613 - 2 Sep 2024
Viewed by 453
Abstract
Accurate short-term load forecasting is critical for enhancing the reliability and stability of regional smart energy systems. However, the inherent challenges posed by the substantial fluctuations and volatility in electricity load patterns necessitate the development of advanced forecasting techniques. In this study, a [...] Read more.
Accurate short-term load forecasting is critical for enhancing the reliability and stability of regional smart energy systems. However, the inherent challenges posed by the substantial fluctuations and volatility in electricity load patterns necessitate the development of advanced forecasting techniques. In this study, a novel short-term load forecasting approach based on a two-stage feature extraction process and a hybrid inverted Transformer model is proposed. Initially, the Prophet method is employed to extract essential features such as trends, seasonality and holiday patterns from the original load dataset. Subsequently, variational mode decomposition (VMD) optimized by the IVY algorithm is utilized to extract significant periodic features from the residual component obtained by Prophet. The extracted features from both stages are then integrated to construct a comprehensive data matrix. This matrix is then inputted into a hybrid deep learning model that combines an inverted Transformer (iTransformer), temporal convolutional networks (TCNs) and a multilayer perceptron (MLP) for accurate short-term load forecasting. A thorough evaluation of the proposed method is conducted through four sets of comparative experiments using data collected from the Elia grid in Belgium. Experimental results illustrate the superior performance of the proposed approach, demonstrating high forecasting accuracy and robustness, highlighting its potential in ensuring the stable operation of regional smart energy systems. Full article
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20 pages, 8579 KiB  
Article
Recognizing Digital Ink Chinese Characters Written by International Students Using a Residual Network with 1-Dimensional Dilated Convolution
by Huafen Xu and Xiwen Zhang
Information 2024, 15(9), 531; https://doi.org/10.3390/info15090531 - 2 Sep 2024
Viewed by 271
Abstract
Due to the complex nature of Chinese characters, junior international students often encounter writing problems related to strokes, components, and their combinations when writing Chinese characters. Digital ink Chinese characters (DICCs) are obtained by sampling the writing trajectory of Chinese characters with a [...] Read more.
Due to the complex nature of Chinese characters, junior international students often encounter writing problems related to strokes, components, and their combinations when writing Chinese characters. Digital ink Chinese characters (DICCs) are obtained by sampling the writing trajectory of Chinese characters with a pen input device. DICCs contain rich information, such as the time and space of strokes and sampling points. Recognizing DICCs is crucial for evaluating and correcting writing errors and enhancing the quality of Chinese character teaching for international students. Here, the paper first employs a one-dimensional dilated convolution to digital ink Chinese character recognition (DICCR) and proposes a novel residual network with one-dimensional dilated convolution (1-D ResNetDC). The 1-D ResNetDC not only utilizes multi-scale convolution kernels, but also employs different dilation rates on a single-scale convolution kernel to obtain information from various ranges. Additionally, residual connections facilitate the training of deep one-dimensional convolutional neural networks. Moreover, the paper proposes a more expressive ten-dimensional feature representation that includes spatial, temporal, and writing direction information for each sampling point, thereby improving classification accuracy. Because the DICC dataset of international students is small and unbalanced, the 1-D ResNetDC is pre-trained on the published available dataset. The experiments demonstrate that our approach is effective and superior. This model features a compact architecture, a reduced number of parameters, and excellent scalability. Full article
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18 pages, 3753 KiB  
Article
New Fault Diagnosis Method for Rolling Bearings Based on Improved Residual Shrinkage Network Combined with Transfer Learning
by Tieyang Sun and Jianxiong Gao
Sensors 2024, 24(17), 5700; https://doi.org/10.3390/s24175700 - 1 Sep 2024
Viewed by 499
Abstract
The fault diagnosis of rolling bearings is faced with the problem of a lack of fault data. Currently, fault diagnosis based on traditional convolutional neural networks decreases the diagnosis rate. In this paper, the developed adaptive residual shrinkage network model is combined with [...] Read more.
The fault diagnosis of rolling bearings is faced with the problem of a lack of fault data. Currently, fault diagnosis based on traditional convolutional neural networks decreases the diagnosis rate. In this paper, the developed adaptive residual shrinkage network model is combined with transfer learning to solve the above problems. The model is trained on the Case Western Reserve dataset, and then the trained model is migrated to a small-sample dataset with a scaled-down sample size and the Jiangnan University bearing dataset to conduct the experiments. The experimental results show that the proposed method can efficiently learn from small-sample datasets, improving the accuracy of the fault diagnosis of bearings under variable loads and variable speeds. The adaptive parameter-rectified linear unit is utilized to adapt the nonlinear transformation. When rolling bearings are in operation, noise production is inevitable. In this paper, soft thresholding and an attention mechanism are added to the model, which can effectively process vibration signals with strong noise. In this paper, the real noise is simulated by adding Gaussian white noise in migration task experiments on small-sample datasets. The experimental results show that the algorithm has noise resistance. Full article
(This article belongs to the Section Fault Diagnosis & Sensors)
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13 pages, 11313 KiB  
Article
An Effective Res-Progressive Growing Generative Adversarial Network-Based Cross-Platform Super-Resolution Reconstruction Method for Drone and Satellite Images
by Hao Han, Wen Du, Ziyi Feng, Zhonghui Guo and Tongyu Xu
Drones 2024, 8(9), 452; https://doi.org/10.3390/drones8090452 - 1 Sep 2024
Viewed by 492
Abstract
In recent years, accurate field monitoring has been a research hotspot in the domains of aerial remote sensing and satellite remote sensing. In view of this, this study proposes an innovative cross-platform super-resolution reconstruction method for remote sensing images for the first time, [...] Read more.
In recent years, accurate field monitoring has been a research hotspot in the domains of aerial remote sensing and satellite remote sensing. In view of this, this study proposes an innovative cross-platform super-resolution reconstruction method for remote sensing images for the first time, aiming to make medium-resolution satellites capable of field-level detection through a super-resolution reconstruction technique. The progressive growing generative adversarial network (PGGAN) model, which has excellent high-resolution generation and style transfer capabilities, is combined with a deep residual network, forming the Res-PGGAN model for cross-platform super-resolution reconstruction. The Res-PGGAN architecture is similar to that of the PGGAN, but includes a deep residual module. The proposed Res-PGGAN model has two main benefits. First, the residual module facilitates the training of deep networks, as well as the extraction of deep features. Second, the PGGAN structure performs well in cross-platform sensor style transfer, allowing for cross-platform high-magnification super-resolution tasks to be performed well. A large pre-training dataset and real data are used to train the Res-PGGAN to improve the resolution of Sentinel-2’s 10 m resolution satellite images to 0.625 m. Three evaluation metrics, including the structural similarity index metric (SSIM), the peak signal-to-noise ratio (PSNR), and the universal quality index (UQI), are used to evaluate the high-magnification images obtained by the proposed method. The images generated by the proposed method are also compared with those obtained by the traditional bicubic method and two deep learning super-resolution reconstruction methods: the enhanced super-resolution generative adversarial network (ESRGAN) and the PGGAN. The results indicate that the proposed method outperforms all the comparison methods and demonstrates an acceptable performance regarding all three metrics (SSIM/PSNR/UQI: 0.9726/44.7971/0.0417), proving the feasibility of cross-platform super-resolution image recovery. Full article
(This article belongs to the Section Drones in Agriculture and Forestry)
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18 pages, 5236 KiB  
Article
A Multi-Branch Feature Extraction Residual Network for Lightweight Image Super-Resolution
by Chunying Liu, Xujie Wan and Guangwei Gao
Mathematics 2024, 12(17), 2736; https://doi.org/10.3390/math12172736 - 1 Sep 2024
Viewed by 361
Abstract
Single-image super-resolution (SISR) seeks to elucidate the mapping relationships between low-resolution and high-resolution images. However, high-performance network models often entail a significant number of parameters and computations, presenting limitations in practical applications. Therefore, prioritizing a light weight and efficiency becomes crucial when applying [...] Read more.
Single-image super-resolution (SISR) seeks to elucidate the mapping relationships between low-resolution and high-resolution images. However, high-performance network models often entail a significant number of parameters and computations, presenting limitations in practical applications. Therefore, prioritizing a light weight and efficiency becomes crucial when applying image super-resolution (SR) to real-world scenarios. We propose a straightforward and efficient method, the Multi-Branch Feature Extraction Residual Network (MFERN), to tackle lightweight image SR through the fusion of multi-information self-calibration and multi-attention information. Specifically, we have devised a Multi-Branch Residual Feature Fusion Module (MRFFM) that leverages a multi-branch residual structure to succinctly and effectively fuse multiple pieces of information. Within the MRFFM, we have designed the Multi-Scale Attention Feature Fusion Block (MAFFB) to adeptly extract features via convolution and self-calibration attention operations. Furthermore, we introduce a Dual Feature Calibration Block (DFCB) to dynamically fuse feature information using dynamic weight values derived from the upper and lower branches. Additionally, to overcome the limitation of convolution in solely extracting local information, we incorporate a Transformer module to effectively integrate global information. The experimental results demonstrate that our MFERN exhibits outstanding performance in terms of model parameters and overall performance. Full article
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17 pages, 4615 KiB  
Article
An Optimized Object Detection Algorithm for Marine Remote Sensing Images
by Yougui Ren, Jialu Li, Yubin Bao, Zhibin Zhao and Ge Yu
Mathematics 2024, 12(17), 2722; https://doi.org/10.3390/math12172722 - 31 Aug 2024
Viewed by 315
Abstract
In order to address the challenge of the small-scale, small-target, and complex scenes often encountered in offshore remote sensing image datasets, this paper employs an interpolation method to achieve super-resolution-assisted target detection. This approach aligns with the logic of popular GANs and generative [...] Read more.
In order to address the challenge of the small-scale, small-target, and complex scenes often encountered in offshore remote sensing image datasets, this paper employs an interpolation method to achieve super-resolution-assisted target detection. This approach aligns with the logic of popular GANs and generative diffusion networks in terms of super-resolution but is more lightweight. Additionally, the image count is expanded fivefold by supplementing the dataset with DOTA and data augmentation techniques. Framework-wise, based on the Faster R-CNN model, the combination of a residual backbone network and pyramid balancing structure enables our model to adapt to the characteristics of small-target scenarios. Moreover, the attention mechanism, random anchor re-selection strategy, and the strategy of replacing quantization operations with bilinear interpolation further enhance the model’s detection capability at a low cost. Ablation experiments and comparative experiments show that, with a simple backbone, the algorithm in this paper achieves a mAP of 71.2% on the dataset, an improvement in accuracy of about 10% compared to the Faster R-CNN algorithm. Full article
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21 pages, 6847 KiB  
Article
Hyperspectral Anomaly Detection Based on Spectral Similarity Variability Feature
by Xueyuan Li and Wenjing Shang
Sensors 2024, 24(17), 5664; https://doi.org/10.3390/s24175664 - 30 Aug 2024
Viewed by 216
Abstract
In the traditional method for hyperspectral anomaly detection, spectral feature mapping is used to map hyperspectral data to a high-level feature space to make features more easily distinguishable between different features. However, the uncertainty in the mapping direction makes the mapped features ineffective [...] Read more.
In the traditional method for hyperspectral anomaly detection, spectral feature mapping is used to map hyperspectral data to a high-level feature space to make features more easily distinguishable between different features. However, the uncertainty in the mapping direction makes the mapped features ineffective in distinguishing anomalous targets from the background. To address this problem, a hyperspectral anomaly detection algorithm based on the spectral similarity variability feature (SSVF) is proposed. First, the high-dimensional similar neighborhoods are fused into similar features using AE networks, and then the SSVF are obtained using residual autoencoder. Finally, the final detection of SSVF was obtained using Reed and Xiaoli (RX) detectors. Compared with other comparison algorithms with the highest accuracy, the overall detection accuracy (AUCODP) of the SSVFRX algorithm is increased by 0.2106. The experimental results show that SSVF has great advantages in both highlighting anomalous targets and improving separability between different ground objects. Full article
(This article belongs to the Special Issue Advanced Optical Sensors Based on Machine Learning)
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45 pages, 3449 KiB  
Review
Non-Muscle Myosin II A: Friend or Foe in Cancer?
by Wasim Feroz, Briley SoYoung Park, Meghna Siripurapu, Nicole Ntim, Mary Kate Kilroy, Arwah Mohammad Ali Sheikh, Rosalin Mishra and Joan T. Garrett
Int. J. Mol. Sci. 2024, 25(17), 9435; https://doi.org/10.3390/ijms25179435 - 30 Aug 2024
Viewed by 259
Abstract
Non-muscle myosin IIA (NM IIA) is a motor protein that belongs to the myosin II family. The myosin heavy chain 9 (MYH9) gene encodes the heavy chain of NM IIA. NM IIA is a hexamer and contains three pairs of peptides, [...] Read more.
Non-muscle myosin IIA (NM IIA) is a motor protein that belongs to the myosin II family. The myosin heavy chain 9 (MYH9) gene encodes the heavy chain of NM IIA. NM IIA is a hexamer and contains three pairs of peptides, which include the dimer of heavy chains, essential light chains, and regulatory light chains. NM IIA is a part of the actomyosin complex that generates mechanical force and tension to carry out essential cellular functions, including adhesion, cytokinesis, migration, and the maintenance of cell shape and polarity. These functions are regulated via light and heavy chain phosphorylation at different amino acid residues. Apart from physiological functions, NM IIA is also linked to the development of cancer and genetic and neurological disorders. MYH9 gene mutations result in the development of several autosomal dominant disorders, such as May-Hegglin anomaly (MHA) and Epstein syndrome (EPS). Multiple studies have reported NM IIA as a tumor suppressor in melanoma and head and neck squamous cell carcinoma; however, studies also indicate that NM IIA is a critical player in promoting tumorigenesis, chemoradiotherapy resistance, and stemness. The ROCK-NM IIA pathway regulates cellular movement and shape via the control of cytoskeletal dynamics. In addition, the ROCK-NM IIA pathway is dysregulated in various solid tumors and leukemia. Currently, there are very few compounds targeting NM IIA, and most of these compounds are still being studied in preclinical models. This review provides comprehensive evidence highlighting the dual role of NM IIA in multiple cancer types and summarizes the signaling networks involved in tumorigenesis. Furthermore, we also discuss the role of NM IIA as a potential therapeutic target with a focus on the ROCK-NM IIA pathway. Full article
(This article belongs to the Section Molecular Oncology)
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14 pages, 3665 KiB  
Article
An Irregular Pupil Localization Network Driven by ResNet Architecture
by Genjian Yang, Wenbai Chen, Peiliang Wu, Jianping Gou and Xintong Meng
Mathematics 2024, 12(17), 2703; https://doi.org/10.3390/math12172703 - 30 Aug 2024
Viewed by 256
Abstract
The precise and robust localization of pupils is crucial for advancing medical diagnostics and enhancing user experience. Currently, the predominant method for determining the center of the pupil relies on the principles of multi-view geometry, necessitating the simultaneous operation of multiple sensors at [...] Read more.
The precise and robust localization of pupils is crucial for advancing medical diagnostics and enhancing user experience. Currently, the predominant method for determining the center of the pupil relies on the principles of multi-view geometry, necessitating the simultaneous operation of multiple sensors at different angles. This study introduces a single-stage pupil localization network named ResDenseDilateNet, which is aimed at utilizing a single sensor for pupil localization and ensuring accuracy and stability across various application environments. Our network utilizes near-infrared (NIR) imaging to ensure high-quality image output, meeting the demands of most current applications. A unique technical highlight is the seamless integration of the efficient characteristics of the Deep Residual Network (ResNet) with the Dense Dilated Convolutions Merging Module (DDCM), which substantially enhances the network’s performance in precisely capturing pupil features, providing a deep and accurate understanding and extraction of pupil details. This innovative combination strategy greatly improves the system’s ability to handle the complexity and subtleties of pupil detection, as well as its adaptability to dynamic pupil changes and environmental factors. Furthermore, we have proposed an innovative loss function, the Contour Centering Loss, which is specifically designed for irregular or partially occluded pupil scenarios. This method innovatively calculates the pupil center point, significantly enhancing the accuracy of pupil localization and robustness of the model in dealing with varied pupil morphologies and partial occlusions. The technology presented in this study not only significantly improves the precision of pupil localization but also exhibits exceptional adaptability and robustness in dealing with complex scenarios, diverse pupil shapes, and occlusions, laying a solid foundation for the future development and application of pupil localization technology. Full article
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18 pages, 4521 KiB  
Article
Feature Fusion Image Dehazing Network Based on Hybrid Parallel Attention
by Hong Chen, Mingju Chen, Hongyang Li, Hongming Peng and Qin Su
Electronics 2024, 13(17), 3438; https://doi.org/10.3390/electronics13173438 - 30 Aug 2024
Viewed by 261
Abstract
Most of the existing dehazing methods ignore some global and local detail information when processing images and fail to fully combine feature information at different levels, which leads to contrast imbalance and residual haze in the dehazed images. To this end, this article [...] Read more.
Most of the existing dehazing methods ignore some global and local detail information when processing images and fail to fully combine feature information at different levels, which leads to contrast imbalance and residual haze in the dehazed images. To this end, this article proposes a image dehazing network based on hybrid parallel attention feature fusion, called the HPA-HFF network. This network is an optimization of the basic network, FFA-Net. First, the hybrid parallel attention (HPA) module is introduced, which uses parallel connections to mix different types of attention mechanisms, which can not only enhance the extraction and fusion capabilities of global spatial context information but also enhance the expression capabilities of features and have better dehazing effects on uneven distribution of haze. Second, the hierarchical feature fusion (HFF) module is introduced, which dynamically fuses feature maps from different paths to adaptively increase their receptive field and refine and enhance image features. Experimental results demonstrate that the HPA-HFF network proposed in this article is contrasted with eight mainstream dehazing networks on the public dataset RESIDE. The HPA-HFF network achieves the highest PSNR (39.41) and SSIM (0.9967) and obtains a good dehazing effect in subjective vision. Full article
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14 pages, 3462 KiB  
Article
Molecular Characterizations of FAM13A and Its Functional Role in Inhibiting the Differentiation of Goat Intramuscular Adipocytes through RIG-I Receptor Signaling Pathway
by Xuening Li, Li Ran, Yanyan Li, Yong Wang, Yan Xiong, Youli Wang, Jiani Xing and Yaqiu Lin
Genes 2024, 15(9), 1143; https://doi.org/10.3390/genes15091143 - 30 Aug 2024
Viewed by 284
Abstract
The aim of this study was to elucidate the effect of FAM13A on the differentiation of goat intramuscular precursor adipocytes and its mechanism of action. Here, we cloned the CDS region 2094 bp of the goat FAM13A gene, encoding a total of 697 [...] Read more.
The aim of this study was to elucidate the effect of FAM13A on the differentiation of goat intramuscular precursor adipocytes and its mechanism of action. Here, we cloned the CDS region 2094 bp of the goat FAM13A gene, encoding a total of 697 amino acid residues. Functionally, overexpression of FAM13A inhibited the differentiation of goat intramuscular adipocytes with a concomitant reduction in lipid droplets, whereas interference with FAM13A expression promoted the differentiation of goat intramuscular adipocytes. To further investigate the mechanism of FAM13A inhibiting adipocyte differentiation, 104 differentially expressed genes were screened by RNA-seq, including 95 up-regulated genes and 9 down-regulated genes. KEGG analysis found that the RIG-I receptor signaling pathway, NOD receptor signaling pathway and toll-like receptor signaling pathway may affect adipogenesis. We selected the RIG-I receptor signaling pathway enriched with more differential genes as a potential adipocyte differentiation signaling pathway for verification. Convincingly, the RIG-I like receptor signaling pathway inhibitor (HY-P1934A) blocked this pathway to save the phenotype observed in intramuscular adipocyte with FAM13A overexpression. Finally, the upstream miRNA of FAM13A was predicted, and the targeted inhibition of miR-21-5p on the expression of FAM13A gene was confirmed. In this study, it was found that FAM13A inhibited the differentiation of goat intramuscular adipocytes through the RIG-I receptor signaling pathway, and the upstream miRNA of FAM13A (miR-21-5p) promoted the differentiation of goat intramuscular adipocytes. This work extends the genetic regulatory network of IMF deposits and provides theoretical support for improving human health and meat quality from the perspective of IMF deposits. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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27 pages, 11225 KiB  
Article
Identification of Groundwater Contamination Sources Based on a Deep Belief Neural Network
by Borui Wang, Zhifang Tan, Wanbao Sheng, Zihao Liu, Xiaoqi Wu, Lu Ma and Zhijun Li
Water 2024, 16(17), 2449; https://doi.org/10.3390/w16172449 (registering DOI) - 29 Aug 2024
Viewed by 383
Abstract
Groundwater Contamination Source Identification (GCSI) is a crucial prerequisite for conducting comprehensive pollution risk assessments, formulating effective groundwater contamination control strategies, and devising remediation plans. In previous GCSI studies, various boundary conditions were typically assumed to be known variables. However, in many practical [...] Read more.
Groundwater Contamination Source Identification (GCSI) is a crucial prerequisite for conducting comprehensive pollution risk assessments, formulating effective groundwater contamination control strategies, and devising remediation plans. In previous GCSI studies, various boundary conditions were typically assumed to be known variables. However, in many practical scenarios, these boundary conditions are exceedingly complex and difficult to accurately pre-determine. This practice of presuming boundary conditions as known may significantly deviate from reality, leading to errors in identification results. Moreover, the outcomes of GCSI may be influenced by multiple factors or conditions, including the fundamental information about the contamination source boundary conditions of the polluted area. This study primarily focuses on contamination source information and unknown boundary conditions. Innovatively, three deep learning surrogate models, the Deep Belief Neural Network (DBNN), Bidirectional Long Short-Term Memory Networks (BiLSTM), and Deep Residual Neural Network (DRNN), are employed for identification and validation and to simulate the highly no-linear simulation model and directly establish a mapping relationship between the outputs and inputs of the simulation model. This approach enables the direct acquisition of the inverse identification results of the variables based on actual monitoring data, thereby facilitating rapid inverse identification. Furthermore, to account for the uncertainty of noise in monitoring data, the inversion accuracy of these three deep learning methods is compared, and the method with higher accuracy is selected for uncertainty analysis. Multiple experiments were conducted, such as accuracy identification tests, robustness tests, and cross-comparative ablation studies. The results demonstrate that all three deep learning models effectively complete the research tasks, with DBNN showing the most exceptional performance in the experiments. DBNN achieved an R2 value of 0.982, an RMSE of 3.77, and an MAE of 7.56%. Subsequent uncertainty analysis, model robustness, and ablation study further affirm DBNN adaptability to GCSI research tasks. Full article
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