Search Results (100,060)

The yield and quality of cultivated food crops are frequently compromised by the prevalent threat from fungal pathogens that can cause widespread damage in both the pre-harvest and post-harvest stages. This paper investigates the challenges posed by fungal pathogens to the sustainability and yield of essential food crops, leading to significant economic and food security repercussions. The paper critiques the long-standing reliance on synthetic fungicides, emphasizing the environmental and health concerns arising from their widespread and occasionally inappropriate use. In response, the paper explores the potential of biological control agents, specifically endophytic fungi in advancing sustainable agricultural practices. Through their diverse symbiotic relationships with host plants, these fungi exhibit strong antagonistic capabilities against phytopathogenic fungi by producing various bioactive compounds and promoting plant growth. The review elaborates on the direct and indirect mechanisms of endophytic antagonism, such as antibiosis, mycoparasitism, induction of host resistance, and competition for resources, which collectively contribute to inhibiting pathogenic fungal growth. This paper consolidates the crucial role of endophytic fungi, i.e., Acremonium, Alternaria, Arthrinium, Aspergillus, Botryosphaeria, Chaetomium, Cladosporium, Cevidencealdinia, Epicoccum, Fusarium, Gliocladium, Muscodor, Nigrospora, Paecilomyces, Penicillium, Phomopsis, Pichia, Pochonia, Pythium, Ramichloridium, Rosellinia, Talaromyces, Trichoderma, Verticillium, Wickerhamomyces, and Xylaria, in biological control, supported by the evidence drawn from more than 200 research publications. The paper pays particular attention to Muscodor, Penicillium, and Trichoderma as prominent antagonists. It also emphasizes the need for future genetic-level research to enhance the application of endophytes in biocontrol strategies aiming to highlight the importance of endophytic fungi in facilitating the transition towards more sustainable and environmentally friendly agricultural systems. Full article

The Lucena 1 cell line, derived from the human chronic myeloid leukemia cell line K562 under selective pressure of vincristine supplementation, exhibits multidrug resistance (MDR). This study aims to explore and elucidate the underlying mechanisms driving MDR in the Lucena 1 cell line. A proteomic analysis comparing K562 and Lucena 1 revealed qualitative differences, with a focus on the ATP-dependent efflux pump, Translocase ABCB1, a key contributor to drug resistance. Tubulin analysis identified two unique isoforms, Tubulin beta 8B and alpha chain-like 3, exclusive to Lucena 1, potentially influencing resistance mechanisms. Additionally, the association of Rap1A and Krit1 in cytoskeletal regulation and the presence of STAT1, linked to the urea cycle and tumor development, offered insights into Lucena 1’s distinctive biology. The increased expression of carbonic anhydrase I suggested a role in pH regulation. The discovery of COP9, a tumor suppressor targeting p53, further highlighted the Lucena 1 complex molecular landscape. This study offers new insights into the MDR phenotype and its multifactorial consequences in cellular pathways. Thus, unraveling the mechanisms of MDR holds promise for innovating cancer models and antitumor targeted strategies, since inhibiting the P-glycoprotein (P-gp)/ABCB1 protein is not always an effective approach given the associated treatment toxicity. Full article

Klebsiella pneumoniae is an opportunistic Gram-negative bacterium in the Enterobacteriaceae family associated with a wide range of diseases, such as pneumonia, bloodstream infections, meningitis and urinary tract infections. Infections caused by drug-resistant strains of Klebsiella pneumoniae pose a significant threat to the effectiveness of conventional antibiotics. Hence, this has led to the need to explore alternative antimicrobial therapies, especially natural products derived from plant sources. This study assessed the phytochemical composition and antibacterial properties and performed a molecular docking analysis of Henna leaves (Lawsonia inermis L.) extracts on strains of Klebsiella pneumoniae. Crude ethanol and methanol extracts of L. inermis L. were prepared at different concentrations (25, 50, 75 and 100 mg/mL) and tested on extended spectrum beta-lactamases (ESBLs)-producing strains of Klebsiella pneumoniae. Phytocompounds were identified using gas chromatography–mass spectrometry (GC-MS) and further subjected to virtual ligands screening with DataWarrior (v05.02.01) and a molecular docking analysis using AutoDock4.2 (v4.2.6). The active compounds of L. inermis L. were determined by the docking analysis, including phytochemical, physicochemical, pharmacokinetics and docking score. The GC-MS analysis identified 27 phytoconstituents, including ethyl acetate, sclareol, 2-[1,2-dihydroxyethyl]-9-[β-d-ribofuranosyl] hypoxanthine, α-bisabolol and 2-Isopropyl-5-methylcyclohexyl 3-(1-(4-chlorophenyl)-3-oxobutyl)-coumarin-4-yl carbonate. The 27 compounds were then screened for their physicochemical and pharmacokinetic properties. The results revealed that the methanol extracts at 100 mg/mL showed significantly higher (p < 0.05) zones of inhibition (13.7 ± 1.2 mm), while the ethanol extracts at 50 mg/mL were significantly lower (6.3 ± 0.6 mm) compared to all the other treatments. The docking analysis revealed that out of the 27 compounds identified, only twelve (12) compounds have a drug-likeness activity. The 12 compounds were further subjected to docking analysis to determine the binding energies with the CTX-M protein of Klebsiella pneumoniae. Only one compound [CID_440869; (2-[1,2-dihydroxyethyl]-9-[β-d-ribofuranosyl] hypoxanthine)] had the best binding energy of −9.76 kcal/mol; hence, it can be considered a potentially suitable treatment for infections caused by ESBLs-producing strains of Klebsiella pneumoniae. This study has demonstrated that L. inermis L. extracts have antibacterial effects. Further research could explore the potential antimicrobial applications of L. inermis L. extracts to many bacterial strains. Full article

Methicillin-resistant Staphylococcus aureus (MRSA) has been included by the World Health Organization in its list of “priority pathogens” because of its widespread prevalence and the severity of the infections it causes. The role of food in infections caused by MRSA is unknown, although strains of this microorganism have been detected in various items for human consumption. In order to gain an overview of any possible role of food in MRSA infections, a review was undertaken of studies published between January 2001 and February 2024 relating to MRSA. These comprised research that focused on fish and shellfish, eggs and egg products, foods of vegetable origin, other foodstuffs (e.g., honey or edible insects), and drinking water. In most of these investigations, no prior enrichment was carried out when isolating strains. Three principal methods were used to confirm the presence of MRSA, namely amplification of the mecA gene by PCR, amplification of the mecA and the mecC genes by PCR, and disc diffusion techniques testing susceptibility to cefoxitin (30 μg) and oxacillin (1 μg). The great diversity of methods used for the determination of MRSA in foods and water makes comparison between these research works difficult. The prevalence of MRSA varied according to the food type considered, ranging between 0.0% and 100% (average 11.7 ± 20.3%) for fish and shellfish samples, between 0.0% and 11.0% (average 1.2 ± 3.5%) for egg and egg products, between 0.0% and 20.8% (average 2.5 ± 6.8%) for foods of vegetable origin, between 0.6% and 29.5% (average 28.2 ± 30.3%) for other foodstuffs, and between 0.0% and 36.7% (average 17.0 ± 14.0%) for drinking water. Full article

Drought is one of the main abiotic stresses affecting plant growth and development. Reduced plant yield and quality are primarily caused by the reductions in photosynthesis, mineral uptake, metabolic disorders, damages from the increased production of reactive oxygen species, and many other disruptions. Plants utilize drought resistance mechanisms as a defense strategy, and the systems’ activation is dependent upon several factors, including plant genotype, onthogenesis phase, drought intensity and duration, and the season in which the drought occurs. Impatiens walleriana is a worldwide popular flowering plant recognized for its vibrant flower colors, and is an indispensable plant in pots, gardens and other public areas. It prefers well-draining, moisturized soil, and does not perform well in overly dry or waterlogged conditions. Consequently, inadequate water supply is a common problem for this plant during production, transportation, and market placement, which has a substantial impact on plant performance overall. This review article outlines certain features of morphological, physiological, and molecular alterations induced by drought in ornamental, drought-sensitive plant species I. walleriana, as well as research carried out to date with the aim to improve the drought tolerance. Stress proteins aquaporins and dehydrins, whose molecular structure was described for the first time in this plant species, are highlighted specifically for their role in drought stress. Furthermore, the effective improvement of drought tolerance in I. walleriana by exogenous application of Plant Growth Regulators and Plant Growth-Promoting Bacteria is discussed in detail. Finally, this review can provide valuable insights for improving plant resilience and productivity in the face of water scarcity, which is critical for sustainable agriculture and horticulture. Full article

There is a high demand for plasma-resistant coatings that prevent the corrosion of the internal ceramic components of plasma etching equipment, thereby reducing particle contamination and process drift. Yttrium oxyfluoride (YOF) coatings were prepared using atmospheric plasma spraying (APS) with commercially available YOF/YF₃ powder mixtures; namely YOF 3%, YOF 6%, and YOF 9%. The etching behaviour of YOF and yttrium oxide (Y₂O₃) coatings was investigated using an inductively coupled plasma consisting of NF₃/He. X-ray photoelectron spectroscopy (XPS) showed that the YOF 6% coating had the thickest fluorinated layer. The scanning electron microscope (SEM) examination revealed that the YOF 6% coating showed exceptional resistance to erosion and generated a reduced quantity of contaminated particles in comparison to Y₂O₃. Consequently, it is more suitable as a protective material for the inner wall of reactors. The YOF coatings exhibit excellent stability and high resistance to erosion, indicating their appropriateness for use in the semiconductor industry. Full article

To investigate the seismic behavior of composite columns with high-strength concrete-filled steel tube flanges and honeycomb steel webs (STHHC) after being subjected to freeze-thaw cycles, 36 full-scale STHHCs were designed with the following main parameters: the shear span ratio (λ_s), the axial compression ratio (n₀), the number of freeze-thaw cycles (N_c), the concrete cubic compression strength (f_cu), and the steel ratio of the section (α_s). Compared with existing experimental data, the validity of the finite element modeling method was verified. Parameter analysis was conducted on 36 full-scale STHHCs to obtain the hysteresis curve of the composite columns and to clarify the impact of the different parameters on the skeleton curve, the energy dissipation capacity, the stiffness degradation, and the ductility of the composite columns. The results showed that the hysteresis curves of all specimens after freeze-thaw cycles exhibited an ideal shuttle shape, reflecting that this kind of composite column has good energy dissipation ability and freeze-thaw resistance. The specimens’ maximum bulging deformation and maximum stress both occurred at the column base. Finally, the restoring force model of this kind of composite column is therefore established, and design recommendations based on these results are proposed. Full article

Salt stress is one of the environmental stresses that significantly reduces crop productivity and quality worldwide. Methods to overcome salt stress include developing salt-resistant crops by inserting various resistance genes or to diagnosing and responding to the effects of salt stress at an early stage. In this study, we investigate the effects of salinity stress on growth, photosynthetic efficiency, and metabolic changes in Brussels sprouts (Brassica oleracea var. gemmifera). Fresh weight and leaf area decreased significantly with increasing NaCl concentration, indicating that salinity stress has a detrimental effect on plant growth. However, chlorophyll fluorescence parameters did not show significant changes, suggesting that photosynthetic efficiency was not significantly affected over 10 days. Fourier transform infrared (FTIR) spectroscopy revealed notable metabolic adjustments, especially in lipids, plastids, proteins, and carbohydrates, indicating biosynthesis of protective compounds such as anthocyanins and proline in response to salinity stress. Pearson correlation analysis confirmed a strong relationship between NaCl concentration and the observed physiological and metabolic changes. The findings highlight the potential of FTIR spectroscopy as a non-destructive tool for early detection of salinity stress and timely intervention to improve crop resilience and yield. This study highlights the widespread application of FTIR spectroscopy in agricultural research to manage abiotic stresses in crops. Full article

Cold-formed steel channel (CFSC) sections have gained widespread adoption in building construction due to their advantageous properties, including superior energy efficiency, expedited construction timelines, environmental sustainability, material efficiency, and ease of transportation. This study presents a numerical investigation into the axial compressive behavior of CFSC section columns. A rigorously developed finite element model for CFSC sections was validated against existing experimental data from the literature. Upon validation, the model was employed for an extensive parametric analysis encompassing a dataset of 208 CFSC members. Furthermore, the efficacy of the design methodologies outlined in the AISI Specification and AS/NZS Standard were evaluated by comparing the axial load capacities obtained from the numerically generated data with the results of four previously conducted experimental tests. The findings reveal that the codified design equations, based on nominal compressive resistances determined using the current direct strength method, exhibit a conservative bias. On average, these equations underestimate the actual load capacities of CFSC section columns by approximately 11.5%. Additionally, this investigation explores the influence of eccentricity, cross-sectional dimensions, and the point-of-load application on the axial load capacity of CFSC columns. The results demonstrate that a decrease in section thickness, an increase in column length, and a higher degree of eccentricity significantly reduce the axial capacity of CFSC columns. Full article

Rubisco small subunit (RbcS), a core component with crucial effects on the structure and kinetic properties of the Rubisco enzyme, plays an important role in response to plant growth, development, and various stresses. Although Rbcs genes have been characterized in many plants, their muti-functions in soybeans remain elusive. In this study, a total of 11 GmRbcS genes were identified and subsequently divided into three subgroups based on a phylogenetic relationship. The evolutionary analysis revealed that whole-genome duplication has a profound effect on GmRbcSs. The cis-acting elements responsive to plant hormones, development, and stress-related were widely found in the promoter region. Expression patterns based on the RT-qPCR assay exhibited that GmRbcS genes are expressed in multiple tissues, and notably Glyma.19G046600 (GmRbcS8) exhibited the highest expression level compared to other members, especially in leaves. Moreover, differential expressions of GmRbcS genes were found to be significantly regulated by exogenous plant hormones, demonstrating their potential functions in diverse biology processes. Finally, the function of GmRbcS8 in enhancing soybean resistance to soybean mosaic virus (SMV) was further determined through the virus-induced gene silencing (VIGS) assay. All these findings establish a strong basis for further elucidating the biological functions of RbcS genes in soybeans. Full article

Asian corn borer (ACB), Ostrinia furnacalis, is an important agricultural pest affecting maize production in southwestern China, but knowledge of the toxic effect of Bt maize on the pest has been insufficient until now. In this study, we determined the susceptibility of ACB to Cry1Ab, Vip3Aa, and their complex proteins and evaluated the efficacy of Chinese domestic Bt-(Cry1Ab+Vip3Aa) maize (“DBN3601T” event) against the pest in Yunnan Province of southwestern China. The susceptible bioassay indicated that the LC₅₀ values of the Cry1Ab and Cry1Ab+Vip3Aa proteins expressed by the Bt maize varieties against ACB larvae were 51.42 and 46.85 ng/g, respectively; however, the ACB larva was insensitive to the Vip3Aa protein. The Cry1Ab+Vip3Aa protein contents in V6–V8 leaves, VT tassels, R1 silks, R2 kernels, R3 stalks and R3 cobs of the Bt-(Cry1Ab+Vip3Aa) maize were 114.20, 30.69, 3.77, 8.92, 11.09 and 10.99 μg/g, respectively. The larval feeding test indicated that the Bt-(Cry1Ab+Vip3Aa) maize was more toxic to the early instar larvae, and the survival time of larvae fed on the leaves was the shortest, while it survived the longest on stalks. The identification of maize resistance levels in the field showed that both larval density and plant damage score of Bt-(Cry1Ab+Vip3Aa) maize were significantly lower than those in conventional maize. It is concluded that the Bt-(Cry1Ab+Vip3Aa) maize can be used for control of the ACB in southwestern China. Full article

Additive Manufacturing (AM) is advancing technologically towards the production of components for high-demand mechanical applications with stringent dimensional accuracy, leveraging metallic and ceramic raw materials. The AM process for ceramic components, known as Ultraviolet Laser Stereolithography (SLA), enables the fabrication of unique parts or small batches without substantial investments in molds and dies, and avoids the problems associated with traditional manufacturing, which involves multiple stages and final machining for precision. This study addresses the need to produce reference elements or targets for metrological applications, including verification, adjustment, or calibration of 3D scanners and mid- to high-range optical sensors. Precision spheres are a primary geometry in this context due to their straightforward mathematical definition, facilitating rapid and accurate error detection in equipment. Our objective is to exploit this novel SLA process along with the advantageous optical properties of technical ceramics (such as being white, matte, lightweight, and corrosion-resistant) to materialize these reference objects. Specifically, this work involves the fabrication of alumina hemispheres using SLA. The manufacturing process incorporates four design variables (wall thickness, support shape, fill type, and orientation) and one manufacturing variable (the arrangement of spheres on the printing tray). To evaluate the impact of the design variables, dimensional and geometric parameters (GD&T), including diameters, form errors, and their distribution on the surface of the sphere, have been characterized. These measurements are conducted with high accuracy using a Coordinate Measuring Machine (CMM). The study also examines the influence of these variables in the dimensional and geometric accuracy of the spheres. Correlations between various parameters were identified, specifically highlighting critical factors affecting process precision, such as the position of the piece on the print tray and the wall thickness value. The smallest diameter errors were recorded at the outermost positions of the tray (rear and front), while the smallest shape errors were found at the central position, in both cases with errors in the range of tens of micrometers. In any case, the smallest deformations were observed with the highest wall thickness (2 mm). Full article

Zirconium alloys possess excellent wear resistance, which ensures the durability and longevity of the components, making them widely used in medical and other fields. To enhance the functionality of these materials, it is often necessary to fabricate functional microstructures on their surfaces. Electrochemical machining (ECM) techniques demonstrate excellent machining performance for these metals, particularly in the processing of microstructures on complex curved surfaces. However, ECM often faces challenges due to the fluid nature of the electrolyte, resulting in low machining accuracy and localization. This paper proposes a novel method for fabricating complex patterned microstructures using a maskless electrochemical direct writing technique with a polyacrylamide (PAM) polymer electrolyte. By leveraging the non-Newtonian properties of PAM, this method effectively confines the electrolyte to specific areas, thus addressing the issue of poor localization in traditional ECM and reducing stray corrosion. To elucidate the electrochemical removal mechanism of Zr702 in the presence of PAM, polarization curves, viscosity characteristics, and current efficiency parameters were analyzed. Additionally, an experimental study was conducted using a custom-designed nozzle structure. The results showed that the PAM electrolyte could effectively reduce the $EF$, positively impacting machining accuracy and localization. By controlling the nozzle’s motion trajectory, complex microstructures were successfully fabricated through direct writing, demonstrating promising application prospects. Full article

This study evaluates the crack performance of stone mastic asphalt (SMA) mixtures according to the performance of a modified asphalt binder, evaluated based on the asphalt performance grade (PG) and the elastic recovery of multiple stress creep and recovery (MSCR) according to AASHTO M 320 and T 350. The cracking performance of the mixture was evaluated using the asphalt mixture performance tester (AMPT) according to AASHTO T 378 and T 400 through dynamic modulus and direct tension cyclic fatigue tests. Furthermore, the recently developed viscoelastic continuum damage (VECD) theory was utilized to evaluate the cyclic fatigue index parameter (apparent damage capacity, Sapp) and the permissible heavy vehicle class. For performance evaluation, six modified asphalt mixtures were prepared and tested using SMA aggregate gradation with a nominal maximum aggregate size (NMAS) of 10 mm. The MSCR test results revealed that, of the six asphalt mixtures, the rubber-based PG76-28 exhibited the least initial strain and the highest elastic recovery. The dynamic modulus test results demonstrated that using a rubber-based modifier increased the elastic modulus at high temperatures and decreased it at low temperatures, thereby enhancing resistance to plastic deformation in the summer and reducing low-temperature cracking in the winter. Finally, the correlation between the Sapp performance index and the elastic recovery of modified asphalt and the number of direct tension cyclic loads until failure of the mixture was evaluated as 0.87 and 0.76, respectively. Full article

Helicobacter pylori (H. pylori) infection is a significant global health concern, affecting approximately 50% of the world’s population and leading to gastric ulcers, gastritis, and gastric cancer. The increase in antibiotic resistance has compromised the efficacy of existing therapeutic regimens, necessitating novel approaches for effective eradication. This study aimed to develop a targeted liposomal drug delivery system incorporating furazolidone and N-acetylcysteine (NAC) to enhance mucopenetration and improve Helicobacter pylori eradication. Liposomes were formulated with furazolidone, NAC, and Pluronic F-127 using a modified reverse-phase evaporation technique. The formulations were categorized based on charge as neutral, negative, and positive and tested for mucopenetration using a modified silicon tube method with coumarin-6 as a fluorescent marker. The encapsulation efficiency and particle size were analyzed using HPLC and an Izon q-nano particle size analyzer. The results indicated that charged liposomes showed a higher encapsulation efficiency than neutral liposomes with Pluronic F-127. Notably, combining furazolidone with 1% NAC achieved complete eradication of H. pylori in 2.5 h, compared to six hours without NAC. The findings of this study suggest that incorporating NAC and Pluronic F-127 into liposomal formulations significantly enhances mucopenetration and antimicrobial efficacy. Full article