Search Results (70,434)

The Arabian Gulf, a semi-enclosed basin in the Middle East, connects to the Indian Ocean through the Strait of Hormuz and is surrounded by seven arid countries. This study examines the water cycle of the Gulf and its surrounding areas using data from the GRACE and GRACE Follow-On missions, along with ERA5 atmospheric reanalysis data, from 05/2002 to 05/2017 and from 07/2018 to 12/2023. Our findings reveal a persistent water deficit due to high evaporation rates, averaging 370 ± 3 km³/year, greatly surpassing precipitation, which accounts for only 15% of the evaporative loss. Continental runoff provides one-fifth of the needed water, while the remaining deficit, approximately 274 ± 10 km³/year, is balanced by net inflow of saltwater from the Indian Ocean. Seasonal variations show the lowest net inflow of 26 ± 49 km³/year in March and the highest of 586 ± 53 km³/year in November, driven by net evaporation, continental input, and changes in the Gulf’s water budget. This study highlights the complex hydrological dynamics influenced by climate patterns and provides a baseline for future research in the region, which will be needed to quantify the expected changes in the hydrological cycle due to climate change. Full article

This paper deals with the study of torsional energy (total squared torsion) at infinitesimal bending of curves and knots in three dimensional Euclidean space. During bending, the curve is subject to change, and its properties are changed. The effect that deformation has on the curve is measured by variations. Here, we observe the infinitesimal bending of the second order and variations of the first and the second order that occur in this occasion. The subjects of study are curves and knots, in particular torus knots. We analyze various examples both analytically and graphically, using our own calculation and visualization software tool. Full article

Background: In pigs, a hair whorl refers to hairs that form a ring of growth around the direction of the hair follicle at the dorsal hip. In China, a hair whorl is considered a negative trait that affects marketing, and no studies have been conducted to demonstrate whether hair whorl affects pig performance and provide an explanation for its genetic basis. Methods: Performance-measured traits and slaughter-measured traits of hair whorl and non-hair whorl pigs were differentially analyzed, followed by genome-wide association analysis (GWAS) and copy number variation (CNV) methods to investigate the genetic basis of hair whorl in pigs. Results: Differential analysis of 2625 pigs (171 hair whorl and 2454 non-hair whorl) for performance measures showed that hair whorl and non-hair whorl pigs differed significantly (p < 0.05) in traits such as live births, total litter size, and healthy litter size (p < 0.05), while differential analysis of carcass and meat quality traits showed a significant difference only in the 45 min pH (p = 0.0265). GWAS identified 4 SNP loci significantly associated with the hair whorl trait, 2 of which reached genome-significant levels, and 23 candidate genes were obtained by annotation with the Ensembl database. KEGG and GO enrichment analyses showed that these genes were mainly enriched in the ErbB signaling, endothelial apoptosis regulation, and cell proliferation pathways. In addition, CNV analysis identified 652 differential genes between hair whorl and non-hair whorl pigs, which were mainly involved in the signal transduction, transcription factor activity, and nuclear and cytoplasmic-related pathways. Conclusions: The candidate genes and copy number variation differences identified in this study provide a new theoretical basis for pig breeding efforts. Full article

Herbaceous plants influence soil hydraulic conductivity by changing soil macropore structure through their root systems, but the effects of different root types on macropore structure have yet to be clarified. In this study, soil column experiments were conducted to investigate temporal variations in soil macropores and saturated hydraulic conductivity, as influenced by herbaceous plants with fibrous roots (Cynodon dactylon) and taproots (Medicago sativa L.). Computed tomography (CT) scanning was used to quantify soil macropore indices, and the saturated hydraulic conductivity (K_s) was measured from April to November after 3, 5, and 7 months of sowing. The results showed that both soil macropore indices and K_s increased with grass growth. The taproots had macroporosity (MP), macropore diameter (MD), and global connectivity (Γ) values that were 1.94, 2.76, and 2.45 times higher than fibrous roots, which are more efficient at increasing the number of soil macropores (PN). This resulted in higher K_s values for the fibrous roots during the experimental period than for the taproot herbs. For both plants, the top 5 cm of soil had a higher macropore index and K_s values than the bottom layer. The K_s for fibrous roots was most closely related to PN. On the other hand, K_s for the taproot soil column was most closely related to MP and MD. Based on a combination of soil macropore indices, functions were established to predict K_s values for fibrous root and taproot herbaceous plants. Full article

Background/Objectives: Lung cancer remains a global health concern, with substantial variation in patient survival. Despite advances in detection and treatment, the genetic basis for the divergent outcomes is not understood. We investigated germline polymorphisms that modulate overall survival in 1464 surgically resected lung adenocarcinoma patients. Methods: A multivariable Cox proportional hazard model was used to assess the association of more than seven million polymorphisms with overall survival at the 60-month follow-up, considering age, sex, pathological stage, decade of surgery and principal components as covariates. Genes in which variants were identified were studied in silico to investigate functional roles. Results: Six germline variants passed the genome-wide significance threshold. These single nucleotide polymorphisms were mapped to non-coding (intronic) regions on chromosomes 2, 3, and 5. The minor alleles of rs13000315, rs151212827, and rs190923216 (chr. 2, 3 and 5, respectively) were found to be independent negative prognostic factors. All six variants have been reported to regulate the expression of nine genes, seven of which are protein-coding, in different tissues. Survival-associated variants on chromosomes 2 and 3 were already reported to regulate the expression of NT5DC2 and NAGK, with high expression associated with the minor alleles. High NT5DC2 and NAGK expression in lung adenocarcinoma tissue was already shown to correlate with poor overall survival. Conclusions: This study highlights a potential regulatory role of the identified polymorphisms in influencing outcome and suggests a mechanistic link between these variants, gene expression regulation, and lung adenocarcinoma prognosis. Validation and functional studies are warranted to elucidate the mechanisms underlying these associations. Full article

Plantago (plantains, Plantaginaceae) is a cosmopolitan genus including over 250 species used as functional foods, forage, and traditional medicine. Among them, Plantago lanceolata is commonly used as an ingredient of herbal products, but the close similarity to other Plantago species can cause misidentifications with potentially serious consequences for product safety/quality. To test the possibility of developing species-specific barcoding markers, we de novo assembled plastome sequences of individuals of Plantago argentea, Plantago atrata, P. lanceolata, and Plantago maritima. These genomes were characterized in comparison with both previously sequenced conspecific accessions and other publicly available plastomes, thus providing an assessment of both intraspecific and interspecific genetic variation in Plantago plastomes. Additionally, molecular evolutionary analyses indicated that eleven protein-coding genes involved in different plastid functions in Plantago plastomes underwent positive selection, suggesting they might have contributed to enhancing species’ adaptation during the evolutionary history of Plantago. While the most variable mutational hotspots in Plantago plastomes were not suitable for the development of species-specific molecular markers, species-specific polymorphisms could discriminate P. lanceolata from its closest relatives. Taken together, these results highlight the potential of plastome sequencing for the development of molecular markers to improve the identification of species with relevance in herbal products. Full article

Wool, a natural fibre derived from sheep, can present a challenge to wool processing and manufacturing industries because of the variation in fibre traits. Genetic improvement offers one solution to this challenge, and having a better understanding of the genes that affect wool fibre traits is therefore important. Here, we describe ovine KRTAP19-3, a new member of the KAP19 gene family. Phylogenetic analysis revealed its relationship to other known KRTAP19 gene sequences, and an analysis of the nucleotide sequence variation in KRTAP19-3 from 288 sheep of a variety of breeds revealed six unique variant sequences. Among these variants, eleven single nucleotide polymorphisms (SNPs) were detected, with six located in the coding region. Three of these coding region SNPs were non-synonymous and would result in amino acid changes. Associations were observed between the presence of specific sequence variants in Chinese Tan sheep and wool trait variation, particularly an increase in fibre diameter variability in the heterotypic hair fibres. These findings enhance our understanding of the genes that encode sheep wool proteins. Full article

To accurately quantify the variation in concrete carbonation depth, selecting an appropriate mathematical curve model is crucial. Currently prevalent models, such as the Fick model and exponential models, confront limitations in prediction accuracy and range of application. Given that a single curve model struggles to precisely describe the pattern of concrete carbonation, this work introduces a mixed-curve-based prediction model for carbonation depth, effectively integrating the Fick model with a hyperbolic model. Compared to the Fick model, the additional term in the mixed-curve model can be viewed as a reasonable correction to better adapt to the complex and varied conditions of concrete carbonation. This hybrid model transcends the limitations of individual models, enhancing fitting precision and broadening the scope of applicability. The new model boasts a concise structure with only two fitting parameters, facilitating ease of application. To validate its superiority, rigorous comparisons were conducted between the proposed model and existing ones, leveraging experimental data from 10 distinct concrete carbonation scenarios. By comparing the average error, standard deviation, and coefficient of determination across these cases, the new model demonstrates a clear advantage over the Fick model and the exponential model. In terms of fitting errors, the average error and standard deviation of the new model are notably lower than those of the other two models. In terms of the coefficient of determination, the values achieved by the new model in all examples are closer to 1 than those of both the Fick model and the exponential model, underscoring the new model’s superior fitting quality and remarkable stability. This research indicates that the combined model presented in this paper holds promising prospects for widespread application in predicting concrete carbonation depth. Full article

Given the dominant failure mode of steel bridge deck pavement layers, which is flexural–tensile damage, the dynamic modulus parameters conventionally determined through uniaxial compression testing are found to be inadequate for the design or performance analysis of these layers. In order to simulate the actual stress of a pavement structure under wheel load, the four-point bending fatigue test method and uniaxial compression test method are used to measure the dynamic modulus of an epoxy asphalt mixture, and the differences between the two test methods are analyzed. Furthermore, the four-point bending fatigue test is employed to investigate the dynamic modulus and phase angle properties across varying temperatures and frequencies, facilitating the creation of master curves for these properties and utilizing Sigmoidal models to correlate dynamic modulus data at diverse temperature conditions. This study delves into the influence of epoxy resin content, mixture composition, and aging on the dynamic modulus. The experimental results show that the dynamic modulus measured by uniaxial compression exceeds that obtained from bending fatigue tests, with the difference initially increasing and then decreasing as temperature rises. This discrepancy significantly impacts the mechanical calculations of pavement layers, underscoring the importance of selecting the appropriate testing method. Temperature, frequency, and epoxy resin content have pronounced effects on the viscoelastic properties of the mixtures. Specifically, as temperature increases, the dynamic modulus undergoes a decrease, whereas the phase angle exhibits an increase. Additionally, the dynamic modulus augments with an increase in loading frequency, while the phase angle exhibits varied trends with frequency shifts across different temperatures. Both the WLF and Sigmoidal models are effective in constructing master curve representations for the dynamic flexural modulus and phase angle. The incorporation of epoxy resin transforms asphalt from a primarily viscous to a more elastic material, significantly enhancing the viscoelastic properties of the mixture. Notably, mixtures with 50% and 60% epoxy resin content exhibit comparable dynamic moduli and phase angles, while displaying notably superior performance compared to those with 40% epoxy resin content. For large-scale steel bridge deck pavement, 50% epoxy resin content is recommended. Moreover, epoxy asphalt mixtures demonstrate robust aging resistance, with minimal variations in the dynamic modulus and phase angle before and after aging. The research results can enable the acquisition of dynamic modulus and phase angle data in the whole temperature domain and the whole frequency domain, and provide reliable mixed performance parameters for the study of different application environmental performance of steel bridge deck pavement. Full article

The Alaotran gentle lemur (Hapalemur alaotrensis) is one of the world’s most endangered primates and shows low success rates in captive breeding programmes. This study tested biologically relevant scent enrichment using two synthesised mixtures likely to convey information about female fertility on the behaviour of three unsuccessful breeding pairs in captivity. Specifically, we compared the baseline and enrichment periods by combining behavioural observations (n = 240 h) with faecal endocrinology (n = 80 samples), focussing on cortisol and testosterone measurements via enzyme immunoassay techniques. Then, we tested two different mixtures to assess potential behavioural differences and evaluate the effectiveness of olfactory enrichment using scented and unscented cotton strips. Olfactory behaviours differed by sex and enrichment conditions, with both sexes exhibiting increased behaviours during enrichment compared to the baseline. Sexual behaviours increased during the enrichment period, with variations in frequency between males and females depending on the condition. No significant changes were observed in faecal cortisol levels. However, one male showed a significant increase in testosterone during the second enrichment mixture. Nevertheless, overall differences between baseline and enrichment were not significant. Our findings suggest that while the scent enrichment showed limited effectiveness, biologically meaningful scents may trigger species-specific behaviours. Full article

A covalent adaptable network (CAN) of semicrystalline polyolefin blends with triple-shape memory effects was fabricated by the reactive melt blending of maleated polypropylene (mPP) and maleated polyolefin elastomer (mPOE) (50 wt/50 wt) in the presence of a small amount of a tetrafunctional thiol (PETMP) and 1,5,7-triazabicyclo [4,4,0]dec-5-ene (TBD). The polymer blend formed a chemically crosslinked network via the reaction between the thiol group of PETMP and maleic anhydride of both polymers in the blend, which was confirmed by FTIR, the variation of torque during the melt mixing process, a solubility test, and DMA. DSC analysis revealed that the crosslinked polyolefin blends show two distinct crystalline melting transitions corresponding to each component polymer. Improved tensile strength as well as elongation at break were observed in the crosslinked blend as compared to the simple blend, and the mechanical properties were maintained after repeated melt processing. These results suggest that thermoplastic polyolefin blends can be transformed into a high-performance and value-added material with good recyclability and reprocessability. Full article

The period of freezing is an important phenological characteristic of lakes in the Northern Hemisphere, exhibiting higher sensitivity to regional climate changes and aiding in the detection of Earth’s response to climate change. This review systematically examines 1141 articles on seasonal frozen lakes from 1991 to 2021, aiming to understand the seasonal variations and control conditions of ice-covered lakes. For the former, we discussed the physical structure and growth characteristics of seasonal ice cover, changes in water environmental conditions and primary production, accumulation and transformation of CO₂ beneath the ice, and the role of winter lakes as carbon sources or sinks. We also proposed a concept of structural stratification based on the differences in physical properties of ice and solute content. The latter provided an overview of the ice-covered period (−1.2 d decade⁻¹), lake evaporation (+16% by the end of the 21st century), the response of planktonic organisms (earlier spring blooming: 2.17 d year⁻¹) to global climate change, the impact of greenhouse gas emissions on ice-free events, and the influence of individual characteristics such as depth, latitude, and elevation on the seasonal frozen lakes. Finally, future research directions for seasonally ice-covered lakes are discussed. Considering the limited and less systematic research conducted so far, this study aims to use bibliometric methods to synthesize and describe the trends and main research points of seasonal ice-covered lakes so as to lay an important foundation for scholars in this field to better understand the existing research progress and explore future research directions. Full article

Research on White Etching Cracks (WEC) in multiple bearing applications has identified various drivers that cause them. Lubricants and electricity combined with contact mechanics have been proven to catalyze WEC significantly. However, none of these factors solely cause WEC on its own; instead, combinations of factors discretize whether WEC appears or not. Hence, the WEC phenomenon appears to be multidimensional, making WEC still unpredictable. The current paper is about a systematic study using a Deep Grove Ball Bearing test rig to investigate how lubricant chemicals, combined with electricity and variations in oil flow and pressure, lead to WEC formation. It becomes evident that even under critical conditions for WEC formation, increasing oil flow and decreasing contact pressure can prevent WEC. Full article

The present study explored the extraction of cellulose from forest residues of four timber species, namely Cedrela montana Moritz ex Turcz, Buddleja incana Ruiz & Pav, Vallea stipularis L. f. and Myrsine andina (Mez) Pipoly, in the high montane forest of Chimborazo province, Ecuador, for the sustainable utilization of leaves, branches, and flowers. An alkaline extraction method was used on the residues without the need for prior degreasing. An ANOVA analysis was applied to evaluate significant differences in cellulose extraction yields among the species’ residues. The characterization techniques used were Fourier transform infrared spectroscopy (FTIR) and polarized light optical microscopy, which confirmed the successful extraction of cellulose with characteristics comparable to standard cotton cellulose and other traditional species. The results showed significant variations in cellulose yield among the species, with Vallea stipularis L. f achieving the highest yield of 80.83%. The crystallinity of the samples was clearly evidenced by the polarity of the light in the samples during microscopy, demonstrating that the residues can be a viable and sustainable source of cellulose, contributing to a circular economy and reducing the environmental impact of forest waste. Full article

The KALRN gene (encoding kalirin) has been implicated in several neuropsychiatric and neurodegenerative disorders. However, genetic evidence supporting this implication is limited and targeted epigenetic analyses are lacking. Here, we tested associations between epigenetic variation in KALRN and interindividual variation in depressive symptoms (PHQ9) and cognitive (MoCA) performance, in an Italian population cohort (N = 2409; mean (SD) age: 67 (9) years; 55% women). First, we analyzed the candidate region chr3:124584826–124584886 (hg38), within the KALRN promoter, through pyrosequencing of 1385 samples. Then, we widened the investigated region by analyzing 137 CpGs annotated to the whole gene, rescued from epigenome-wide (Illumina EPIC) data from 1024 independent samples from the same cohort. These were tested through stepwise regression models adjusted for age, sex, circulating leukocytes fractions, education, prevalent health conditions and lifestyles. We observed no statistically significant associations with methylation levels in the three CpGs tested through pyrosequencing, or in the gene-wide association analysis with MoCA score. However, we observed a statistically significant association between PHQ9 and cg13549966 (chr3:124106738; β (Standard Error) = 0.28 (0.08), Bonferroni-corrected p = 0.025), located close to the transcription start site of the gene. This association was driven by a polychoric factor tagging somatic depressive symptoms (β (SE) = 0.127 (0.064), p = 0.048). This evidence underscores the importance of studying epigenetic variation within the KALRN gene and the role that it may play in brain diseases, particularly in atypical depression, which is often characterized by somatic symptoms. Full article