A Prominent Role for DC-SIGN+ Dendritic Cells in Initiation and Dissemination of Measles Virus Infection in Non-Human Primates
Figure 4
Macaque DC-SIGN binds mannose structures including MV and transmits MV to CD150+ target cells.
(A) CHO cells were transfected with macDC-SIGN. The mean expression levels of DC-SIGN and CD150 of the parental cell line and transfectants are depicted. Gray areas represent isotype controls. (B) MacDC-SIGN binds mannose and fucose structures on fluorescent beads. Binding was blocked by anti-DC-SIGN (20 ug/ml. (C) CHO transfectants were pre-incubated with anti-DC-SIGN antibodies or isotype control (20 ug/ml) before incubation with FITC-labeled UV-inactivated MV. Binding was measured by flow cytometry. (D) Parental and transfected CHO cells were infected with rMVKSEGFP (MOI 1) and 48 hours post infection EGFP levels were measured in FACS. (E) Cells were incubated with rMVKSEGFP (MOI 3). After 3 hours cells were washed and CD150+ Raji cells were added to the culture. Infection of Raji cells was determined by measuring EGFP in flow cytometry. Transmission was blocked by pre-incubating CHO macDC-SIGN cells with anti-DC-SIGN (20 ug/ml) or mannan (0.25 mg/ml). All data are representatives for at least 2 independent experiments. Error bars represent standard deviations of duplicates.