Species-specific emergence of H7 highly pathogenic avian influenza virus is driven by intrahost selection differences between chickens and ducks
Fig 3
Detection of H7N7-HPHAtag and H7N7-LPFLAGtag in tissues of chickens and ducks at 3 dpi.
(A, B) Viral RNA quantification in tissues harvested at 3 dpi from chickens (A) and ducks (B) inoculated with ~104 EID50 of H7N7-HPHAtag or ~106 EID50 of H7N7-LPFLAGtag. Viral RNA amounts were determined by RT-qPCR targeting the influenza virus matrix segment and expressed as 40-cycle threshold (Ct) normalized/gram tissue. Bars represent the arithmetic mean. The dotted lines distinguish tissues from the respiratory, digestive, and miscellaneous systems. (C, D) Infectious virus titers in tissues from panels (A, B). Infectious titers were determined by endpoint titration in MDCK cells and expressed as log10 TCID50/gram tissue. Bars represent the arithmetic mean of log10 transformed values. Dotted lines similar to (A, B). The horizontal solid lines indicate the limit of detection of the endpoint titration assay per tissue.